scholarly journals Cronotanatognose, utilização da polpa dentária como ferramenta para determinar o tempo de morte: uma revisão

2021 ◽  
Vol 10 (10) ◽  
pp. e353101018862
Author(s):  
Georgiana Ferreira Ramos ◽  
Eskálath Morganna Silva Ferreira ◽  
Juliana Fonseca Moreira da Silva ◽  
Raphael Sanzio Pimenta
Keyword(s):  
Dental Pulp ◽  
Experimental Studies ◽  
High Strength ◽  
The Body ◽  
Post Mortem ◽  
Criminal Cases ◽  
Post Mortem Interval ◽  
Complex Process ◽  
Para Determinar ◽  
The Moment

The post-mortem interval is the period of time that has passed since the death occurred until the moment when the body and /or human remnants are studied. The estimation of this interval is a matter of great relevance in the forensic sphere due to its important role in the resolution of criminal cases. Teeth are fundamental structures in a forensic context due to their high strength and specificity. Therefore, this article aims to present how the dental pulp is used to determine the time of death of the corpse. A systematic review of the scientific literature was carried out with a descriptive and qualitative character, allowing the inclusion of experimental and non-experimental studies for a complete coverage of the analyzed phenomenon. For data collection, online searches were carried out on the bases: SciElo, LILACS and Elsevier. All journal papers that used the selected terms were included in this review. The degradation of dental pulp after death consists of a complex process that has not been fully studied, but with the techniques and changes shown in the text, reliable post-mortem interval results are possible.

scholarly journals Dysregulation of miR-381-3p and miR-23b-3p in skeletal muscle could be a possible estimator of early post-mortem interval in rats

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11102
Author(s):  
Vanessa Martínez-Rivera ◽  
Christian A. Cárdenas-Monroy ◽  
Oliver Millan-Catalan ◽  
Jessica González-Corona ◽  
N. Sofia Huerta-Pacheco ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
In Silico Analysis ◽  
Forensic Analysis ◽  
The Body ◽  
Control Group ◽  
Post Mortem ◽  
Rt Pcr ◽  
Post Mortem Interval ◽  
Silico Analysis

Background The post-mortem interval (PMI) is the time elapsed since the dead of an individual until the body is found, which is relevant for forensic purposes. The miRNAs regulate the expression of some genes; and due to their small size, they can better support degradation, which makes them suitable for forensic analysis. In the present work, we evaluated the gene expression of miR-381-3p, miR-23b-3p, and miR-144-3p in skeletal muscle in a murine model at the early PMI. Methods We designed a rat model to evaluate the early PMI under controlled conditions. This model consisted in 25 rats divided into five groups of rats, that correspond to the 0, 3, 6, 12 and 24 hours of PMI. The 0 h-PMI was considered as the control group. Muscle samples were taken from each rat to analyze the expression of miR-381-3p, miR-23b-3p, and miR-144-3p by quantitative RT-PCR. The gene expression of each miRNA was expressed as Fold Change (FC) and compared among groups. To find the targets of these miRNAs and the pathways where they participate, we performed an in-silico analysis. From the gene targets of miR-381-3p identified in the silico analysis, the EPC1 gene was selected for gene expression analysis by quantitative RT-PCR in these samples. Also, to evaluate if miR-381-3p could predict the early PMI, a mixed effects model was calculated using its gene expression. Results An upregulation of miR-381-3p was found at 24 h-PMI compared with the control group of 0 h-PMI and (FC = 1.02 vs. FC = 1.96; p = 0.0079). This was the opposite for miR-23b-3p, which had a down-regulation at 24 h-PMI compared to 0 h-PMI (FC = 1.22 vs. FC = 0.13; p = 0.0079). Moreover, the gene expression of miR-381-3p increased throughout the first 24 h of PMI, contrary to miR-23b-3p. The targets of these two miRNAs, participate in biological pathways related to hypoxia, apoptosis, and RNA metabolism. The gene expression of EPC1 was found downregulated at 3 and 12 h of PMI, whereas it remained unchanged at 6 h and 24 h of PMI. Using a multivariate analysis, it was possible to predict the FC of miR-381-3p of all but 6 h-PMI analyzed PMIs. Discussion The present results suggest that miR-23b-3p and miR-381-3p participate at the early PMI, probably regulating the expression of some genes related to the autolysis process as EPC1 gene. Although the miR-381-3p gene expression is a potential estimator of PMI, further studies will be required to obtain better estimates.

Use of entomotoxicology in estimating post-mortem interval and determining cause of death

2017 ◽  
Vol 295 ◽  
pp. 56-63
Author(s):  
Joanna Stojak ◽  
Keyword(s):  
Cause Of Death ◽  
Current Knowledge ◽  
The Body ◽  
Toxic Substances ◽  
Post Mortem ◽  
Toxicological Analysis ◽  
Post Mortem Interval ◽  
The Impact

Entomotoxicology allows the estimation of the post-mortem interval and the determination of the cause of death in cases in which the corpse has decomposed and the tissues necessary for toxicological analysis are no longer available. Obtaining information about toxic substances potentially present in the body is possible by isolation of larvae and pupae of true flies (Diptera) and/or adult forms of, e.g., beetles (Coleoptera) present on or near the corpse. This article was intended to summarize the current knowledge in the field of entomotoxicology, including examples from the literature, and to present the impact of selected toxic substances and medicines on the development of necrophagous larvae of insects.

Histological transformations of the dental pulp as possible indicator of post mortem interval: a pilot study

2017 ◽  
Vol 279 ◽  
pp. 251-257 ◽  
Author(s):  
Patricio A. Carrasco ◽  
Claudia I. Brizuela ◽  
Ismael A. Rodriguez ◽  
Samuel Muñoz ◽  
Marianela E. Godoy ◽  
...  
Keyword(s):  
Pilot Study ◽  
Dental Pulp ◽  
Post Mortem ◽  
Post Mortem Interval

Simultaneous analysis of potassium and ammonium ions in the vitreous humour by capillary electrophoresis and their integrated use to infer the post mortem interval (PMI)

2020 ◽  
pp. 002580242093423 ◽  
Author(s):  
Covadonga Palacio ◽  
Rossella Gottardo ◽  
Vito Cirielli ◽  
Giacomo Musile ◽  
Yvane Agard ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Clinical Chemistry ◽  
The Body ◽  
Simultaneous Analysis ◽  
Vitreous Humour ◽  
Ammonium Concentration ◽  
Time Of Death ◽  
Post Mortem ◽  
Ammonium Ions ◽  
Post Mortem Interval

Post-mortem changes of ions in the body fluids have been proposed as an objective tool for inferring the time of death. In particular, the post-mortem increase of potassium concentrations in the vitreous humour has gained great attention in the literature. On the other hand, ammonium, another ion released in post-mortem processes, has received much less attention, potentially due to unresolved analytical issues using current clinical chemistry methods. This paper presents an application of a new analytical approach based on capillary electrophoresis providing the simultaneous analysis of potassium and ammonium ions in the vitreous humour. In addition, to assess the consistency of the post-mortem increase of ammonium concentrations in the vitreous humour, the determination of this ion in the vitreous humour of the two eyes of the same body at the same post-mortem interval has been verified. Vitreous humour was collected from 33 medico-legal cases where the time of death was known exactly. Prior to analysis, all samples were diluted 1:20 with a 40 μg/mL solution of BaCl2 (internal standard). In the study of the variability of the ammonium concentration between the two eyes, no statistically significant differences were found, supporting the hypothesis of an even post-mortem increase of the ion concentrations in this particular biological fluid. Significant correlations of potassium and ammonium ions with the post-mortem interval were found, with r2 of 0.75 and 0.70, respectively.

Estimation of Post-Mortem Interval from Rectal Temperature by Use of Computer

1980 ◽  
Vol 20 (2) ◽  
pp. 115-125 ◽  
Author(s):  
Kouichi Hiraiwa ◽  
Youkichi Ohno ◽  
Fusakuni Kuroda ◽  
Ismail M. Sebetan ◽  
Shigemi Oshida
Keyword(s):  
Ambient Temperature ◽  
Rectal Temperature ◽  
Computer Tomography ◽  
Temperature Curve ◽  
The Body ◽  
Infinite Cylinder ◽  
Post Mortem ◽  
Post Mortem Interval ◽  
Posterior Quarter ◽  
Approximate Equations

The simulation model of the approximate equations derived from the infinite cylinder was used to investigate the applicability of the theoretical curve to the actual one of rectal temperature. The rectum was found by the computer tomography to be near to the junction of the anterior three-quarters and the posterior quarter inside the body, and this result was employed in the simulation. The method of estimating the postmortem interval, in which two recordings of rectal temperature were used, was considered to be useful enough as it can be applied to any case in practice. As reported by Akaishi et al., fluctuation of less than 2 °C in the ambient temperature was shown by the simulation to have little effect on the rectal temperature curve.

scholarly journals Post-mortem Interval and Its Connection with the RNA Degradation from the Dental Pulp in Submerged Teeth

2021 ◽  
Vol 3 (1) ◽  
pp. 68-76
Author(s):  
Bruna Saud Borges ◽  
Thiago J. Dionísio ◽  
Carlos F. Santos ◽  
Ricardo H. Alves da Silva
Keyword(s):  
Fresh Water ◽  
Aquatic Environment ◽  
Dental Pulp ◽  
Rna Degradation ◽  
Post Mortem ◽  
Third Molars ◽  
Time Intervals ◽  
Human Teeth ◽  
Post Mortem Interval ◽  
Quantification Method

Objective: Evaluate the applicability of using the method of quantifying the RNA degradation extracted from dental pulps to estimate the post mortem interval, by simulating drowning conditions with teeth submerged in fresh water and exposed to different time intervals. Material and methods: The sample consisted of 80 human teeth (third molars), which were divided into eight groups and submitted to the aquatic environment, for pre-established periods of three days, and 1, 2, 3, 4, 8, 12 and 16 weeks. After the stipulated time and the recovery of the teeth, the removal of the dental pulp, extraction of the RNA molecule and analysis of the degradation of the molecule were carried out. Results: After the analysis, the highest number of RNA molecule (RIN) found was 6,50 and the results showed very degraded molecules, highlighting the fact that the samples were submitted to the environment, simulating real day-to-day conditions, which may have been a primary factor to justify the results found in this work. Conclusion: RNA degradation quantification method is not applicable, since it was not possible to establish a connection between the degradation of the RNA molecule and the estimation of the post mortem interval.

Impact of Sugar on Vision

2021 ◽  
Author(s):  
Grace Ogbonna ◽  
Rosemary Ehigbo ◽  
Ogbonna Hannah
Keyword(s):  
Oxidative Stress ◽  
Human Body ◽  
Aqueous Humour ◽  
Low Vision ◽  
The Body ◽  
Vision Impairment ◽  
Post Mortem ◽  
Normal Concentration ◽  
Post Mortem Interval ◽  
Patients With Diabetes

Sugar forms an integral part of the human body, and contributes to normal body function. However, sugar in high quantities can be detrimental to the body especially to the eye. In the normal concentration, sugar in the form of glucose is found in the aqueous humour, and tears, and serves to provide nourishment to the avascular cornea, and lens respectively. Sugar at this stage may also be used to determine the post mortem interval of a cadaver. However, when in excess as may be seen in patients with diabetes, sugar can cause oxidative stress to the cornea, lens, and retina resulting in cornea oedema, cataract, retinal aneurysm which can contribute significantly to the prevalence of low vision, and vision impairment.

Work on the pull-out of anchors based on carbon fibers when arranging external reinforcement systems

2019 ◽  
pp. 29-34
Keyword(s):  
Carbon Fibers ◽  
Experimental Studies ◽  
Breaking Load ◽  
The Body ◽  
Pull Out ◽  
External Reinforcement ◽  
Concrete Body ◽  
The Moment ◽  
Two Stages ◽  
The Impact

Reliable anchoring of carbon reinforcement elements into the body of the structure is one of the most important aspects when designing the strengthening of reinforced concrete structures with the system of external reinforcement based on composite materials. The use of carbon fiber anchoring elements in this case is confirmed by numerous tests, but there are no methodological provisions for the calculation and design of these anchors at the moment. Special experimental studies of the parameters of anchors and their effect on the bearing capacity of the anchorage have been conducted for this purpose. The tests were carried out in two stages, during the experiments the breaking load, the size of the concrete pull-out cone, as well as the nature of the destruction of the anchor were recorded. In addition, the length of the anchorage in the concrete body, the pull-out forces, the maximum deformations of the anchor and the length of the anchorage when introducing the anchor fibers in the external reinforcement system have been defined. According to the results of the experiments, the analysis of the results, in particular, various schemes of destruction of anchors and the impact of the depth of the anchor on them, its diameter, as well as the type of binders was carried out.

The rate of RNA degradation in human dental pulp reveals post-mortem interval

2015 ◽  
Vol 130 (3) ◽  
pp. 615-619 ◽  
Author(s):  
Viktor S. Poór ◽  
Dénes Lukács ◽  
Tamás Nagy ◽  
Evelin Rácz ◽  
Katalin Sipos
Keyword(s):  
Dental Pulp ◽  
Rna Degradation ◽  
Post Mortem ◽  
Post Mortem Interval ◽  
Human Dental Pulp

A Good Sentencing Precedent is Hard to Find

2020 ◽  
Vol 32 (3) ◽  
pp. 138-144
Author(s):  
Brian A. Jacobs
Keyword(s):  
United States ◽  
Significant Role ◽  
College Admissions ◽  
The Body ◽  
Federal Law ◽  
Criminal Cases ◽  
Federal Sentencing

In federal criminal cases, federal law requires that judges consider the sentences other courts have imposed in factually similar matters. Courts and parties, however, face significant challenges in finding applicable sentencing precedents because judges do not typically issue written sentencing opinions, and transcripts of sentencings are not readily available in advanced searchable databases. At the same time, particularly since the Supreme Court’s 2005 decision in United States v. Booker, sentencing precedent has come to play a significant role in federal sentencing proceedings. By way of example, this article discusses recent cases involving defendants with gambling addictions, and recent cases involving college admissions or testing fraud. The article explores the ways the parties in those cases have used sentencing precedent in their advocacy, as well as the ways the courts involved have used sentencing precedent to justify their decisions. Given the important role of sentencing precedent in federal criminal cases, the article finally looks at ways in which the body of sentencing law could be made more readily available to parties and courts alike.

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