The rate of RNA degradation in human dental pulp reveals post-mortem interval

2015 ◽  
Vol 130 (3) ◽  
pp. 615-619 ◽  
Author(s):  
Viktor S. Poór ◽  
Dénes Lukács ◽  
Tamás Nagy ◽  
Evelin Rácz ◽  
Katalin Sipos
Keyword(s):  
Dental Pulp ◽  
Rna Degradation ◽  
Post Mortem ◽  
Post Mortem Interval ◽  
Human Dental Pulp

scholarly journals Post-mortem Interval and Its Connection with the RNA Degradation from the Dental Pulp in Submerged Teeth

2021 ◽  
Vol 3 (1) ◽  
pp. 68-76
Author(s):  
Bruna Saud Borges ◽  
Thiago J. Dionísio ◽  
Carlos F. Santos ◽  
Ricardo H. Alves da Silva
Keyword(s):  
Fresh Water ◽  
Aquatic Environment ◽  
Dental Pulp ◽  
Rna Degradation ◽  
Post Mortem ◽  
Third Molars ◽  
Time Intervals ◽  
Human Teeth ◽  
Post Mortem Interval ◽  
Quantification Method

Objective: Evaluate the applicability of using the method of quantifying the RNA degradation extracted from dental pulps to estimate the post mortem interval, by simulating drowning conditions with teeth submerged in fresh water and exposed to different time intervals. Material and methods: The sample consisted of 80 human teeth (third molars), which were divided into eight groups and submitted to the aquatic environment, for pre-established periods of three days, and 1, 2, 3, 4, 8, 12 and 16 weeks. After the stipulated time and the recovery of the teeth, the removal of the dental pulp, extraction of the RNA molecule and analysis of the degradation of the molecule were carried out. Results: After the analysis, the highest number of RNA molecule (RIN) found was 6,50 and the results showed very degraded molecules, highlighting the fact that the samples were submitted to the environment, simulating real day-to-day conditions, which may have been a primary factor to justify the results found in this work. Conclusion: RNA degradation quantification method is not applicable, since it was not possible to establish a connection between the degradation of the RNA molecule and the estimation of the post mortem interval.

Histological transformations of the dental pulp as possible indicator of post mortem interval: a pilot study

2017 ◽  
Vol 279 ◽  
pp. 251-257 ◽  
Author(s):  
Patricio A. Carrasco ◽  
Claudia I. Brizuela ◽  
Ismael A. Rodriguez ◽  
Samuel Muñoz ◽  
Marianela E. Godoy ◽  
...  
Keyword(s):  
Pilot Study ◽  
Dental Pulp ◽  
Post Mortem ◽  
Post Mortem Interval

scholarly journals Cronotanatognose, utilização da polpa dentária como ferramenta para determinar o tempo de morte: uma revisão

2021 ◽  
Vol 10 (10) ◽  
pp. e353101018862
Author(s):  
Georgiana Ferreira Ramos ◽  
Eskálath Morganna Silva Ferreira ◽  
Juliana Fonseca Moreira da Silva ◽  
Raphael Sanzio Pimenta
Keyword(s):  
Dental Pulp ◽  
Experimental Studies ◽  
High Strength ◽  
The Body ◽  
Post Mortem ◽  
Criminal Cases ◽  
Post Mortem Interval ◽  
Complex Process ◽  
Para Determinar ◽  
The Moment

The post-mortem interval is the period of time that has passed since the death occurred until the moment when the body and /or human remnants are studied. The estimation of this interval is a matter of great relevance in the forensic sphere due to its important role in the resolution of criminal cases. Teeth are fundamental structures in a forensic context due to their high strength and specificity. Therefore, this article aims to present how the dental pulp is used to determine the time of death of the corpse. A systematic review of the scientific literature was carried out with a descriptive and qualitative character, allowing the inclusion of experimental and non-experimental studies for a complete coverage of the analyzed phenomenon. For data collection, online searches were carried out on the bases: SciElo, LILACS and Elsevier. All journal papers that used the selected terms were included in this review. The degradation of dental pulp after death consists of a complex process that has not been fully studied, but with the techniques and changes shown in the text, reliable post-mortem interval results are possible.

Characteristics of the number of odontoblasts in human dental pulp post-mortem

2009 ◽  
Vol 193 (1-3) ◽  
pp. 122-126 ◽  
Author(s):  
Marko Vavpotič ◽  
Tomaž Turk ◽  
Draga Štiblar Martinčič ◽  
Jože Balažic
Keyword(s):  
Dental Pulp ◽  
Post Mortem ◽  
Human Dental Pulp

scholarly journals Evaluating the utility of time-lapse imaging in the estimation of post-mortem interval: An Australian case study

2019 ◽  
Vol 1 ◽  
pp. 204-210 ◽  
Author(s):  
Alyson Wilson ◽  
Stanley Serafin ◽  
Dilan Seckiner ◽  
Rachel Berry ◽  
Xanthé Mallett
Keyword(s):  
Time Lapse ◽  
Post Mortem ◽  
Post Mortem Interval ◽  
Australian Case ◽  
Time Lapse Imaging

scholarly journals The Preparation and Characterization of Chitosan-Based Hydrogels Cross-Linked by Glyoxal

Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2449
Author(s):  
Beata Kaczmarek-Szczepańska ◽  
Olha Mazur ◽  
Marta Michalska-Sionkowska ◽  
Krzysztof Łukowicz ◽  
Anna Maria Osyczka
Keyword(s):  
Thermal Stability ◽  
Tannic Acid ◽  
Dental Pulp ◽  
Blood Compatibility ◽  
Dental Pulp Cells ◽  
Preliminary Assessment ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells

In this study, hydrogels based on chitosan cross-linked by glyoxal have been investigated for potential medical applications. Hydrogels were loaded with tannic acid at different concentrations. The thermal stability and the polyphenol-releasing rate were determined. For a preliminary assessment of the clinical usefulness of the hydrogels, they were examined for blood compatibility and in the culture of human dental pulp cells (hDPC). The results showed that after immersion in a polyphenol solution, chitosan/glyoxal hydrogels remain nonhemolytic for erythrocytes, and we also did not observe the cytotoxic effect of hydrogels immersed in tannic acid (TA) solutions with different concentration. Tannic acid was successfully released from hydrogels, and its addition improved material thermal stability. Thus, the current findings open the possibility to consider such hydrogels in clinics.

scholarly journals Upregulation of ETV2 Expression Promotes Endothelial Differentiation of Human Dental Pulp Stem Cells

2021 ◽  
Vol 30 ◽  
pp. 096368972097873
Author(s):  
Jing Li ◽  
Youming Zhu ◽  
Na Li ◽  
Tao Wu ◽  
Xianyu Zheng ◽  
...  
Keyword(s):  
Protein Expression ◽  
Dental Pulp ◽  
Tube Formation ◽  
Endothelial Differentiation ◽  
Cell Source ◽  
Lentiviral Infection ◽  
Mrna And Protein Expression ◽  
Human Dental Pulp

The lack of vasculogenesis often hampers the survivability and integration of newly engineered tissue grafts within the host. Autologous endothelial cells (ECs) are an ideal cell source for neovascularization, but they are limited by their scarcity, lack of proliferative capacity, and donor site morbidity upon isolation. The objective of this study was to determine whether differentiation of human dental pulp stem cells (DPSCs) into the endothelial lineage can be enhanced by recombinant ETV2 overexpression. DPSCs were extracted from fresh dental pulp tissues. ETV2 overexpression in DPSCs was achieved by lentiviral infection and cellular morphological changes were evaluated. The mRNA and protein expression levels of endothelial-specific markers were assessed through quantitative real-time polymerase chain reaction, western blot, immunofluorescence staining, and flow cytometry. The tube formation assay and Matrigel plug assay were also performed to evaluate the angiogenic potential of the ETV2-transduced cells in vitro and in vivo, respectively. Additionally, proteomic analysis was performed to analyze global changes in protein expression following ETV2 overexpression. After lentiviral infection, ETV2-overexpressing DPSCs showed endothelial-like morphology. Compared with control DPSCs, significantly higher mRNA and protein expression levels of endothelial-specific genes, including CD31, VE-Cadherin, VEGFR1, and VEGFR2, were detected in ETV2-overexpressing DPSCs. Moreover, ETV2 overexpression enhanced capillary-like tube formation on Matrigel in vitro, as well as neovascularization in vivo. In addition, comparative proteomic profiling showed that ETV2 overexpression upregulated the expression of vascular endothelial growth factor (VEGF) receptors, which was indicative of increased VEGF signaling. Taken together, our results indicate that ETV2 overexpression significantly enhanced the endothelial differentiation of DPSCs. Thus, this study shows that DPSCs can be a promising candidate cell source for tissue engineering applications.

Chrysin induces osteogenic differentiation of human dental pulp stem cells

2021 ◽  
Vol 400 (2) ◽  
pp. 112466
Author(s):  
J.F. Huo ◽  
M.L. Zhang ◽  
X.X. Wang ◽  
D.H. Zou
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

scholarly journals The Selective Histone Deacetylase Inhibitor MI192 Enhances the Osteogenic Differentiation Efficacy of Human Dental Pulp Stromal Cells

2021 ◽  
Vol 22 (10) ◽  
pp. 5224
Author(s):  
Kenny Man ◽  
Liam Lawlor ◽  
Lin-Hua Jiang ◽  
Xuebin B. Yang
Keyword(s):  
Alkaline Phosphatase ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Dental Pulp ◽  
Selective Inhibitor ◽  
Epigenetic Reprogramming ◽  
Type I ◽  
Human Dental Pulp ◽  
Pre Treatment ◽  
Dose Dependent

The use of human dental pulp stromal cells (hDPSCs) has gained increasing attention as an alternative stem cell source for bone tissue engineering. The modification of the cells’ epigenetics has been found to play an important role in regulating differentiation, with the inhibition of histone deacetylases 3 (HDAC3) being linked to increased osteogenic differentiation. This study aimed to induce epigenetic reprogramming using the HDAC2 and 3 selective inhibitor, MI192 to promote hDPSCs osteogenic capacity for bone regeneration. MI192 treatment caused a time–dose-dependent change in hDPSC morphology and reduction in viability. Additionally, MI192 successfully augmented hDPSC epigenetic functionality, which resulted in increased histone acetylation and cell cycle arrest at the G2/M phase. MI192 pre-treatment exhibited a dose-dependent effect on hDPSCs alkaline phosphatase activity. Quantitative PCR and In-Cell Western further demonstrated that MI192 pre-treatment significantly upregulated hDPSCs osteoblast-related gene and protein expression (alkaline phosphatase, bone morphogenic protein 2, type I collagen and osteocalcin) during osteogenic differentiation. Importantly, MI192 pre-treatment significantly increased hDPSCs extracellular matrix collagen production and mineralisation. As such, for the first time, our findings show that epigenetic reprogramming with the HDAC2 and 3 selective inhibitor MI192 accelerates the osteogenic differentiation of hDPSCs, demonstrating the considerable utility of this MSCs engineering approach for bone augmentation strategies.

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