scholarly journals α-amylase production obtained from Aspergillus niger ATCC 1004 by solid state fermentation using Croton linearifolius residues as substrate

2021 ◽  
Vol 10 (11) ◽  
pp. e510101119891
Author(s):  
Juliana Antunes da Luz ◽  
Dhiéssica dos Santos Ribeiro ◽  
Jabson Menezes Teixeira ◽  
Thamilly Moreira Silva ◽  
Danilo Junqueira Leão ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Cobalt Chloride ◽  
Enzyme Inhibitors ◽  
Incubation Temperature ◽  
Iron Chloride ◽  
Enzymatic Characterization ◽  
Fermentation Time ◽  
Salt Addition

The objective of this work was to optimize the production and characterize α-amylase produced by Aspergillus niger through solid state fermentation, using leaf residues of C. linearifolius as substrate. For optimization, the incubation temperature, initial humidity and fermentation time were combined based on Doehlert experimental design. The highest productivity of the enzyme was 122.88 Ug-1, at 33oC, 70% humidity and 14 days of time. In the enzymatic characterization, the enzyme extract presented pH 5.0 and temperature 50oC, and α-amylase was thermostable up to 60oC, maintaining more than 90% of the activity. In evaluation of the effect of salt addition, sodium carbonate, calcium chloride, iron chloride, and cobalt chloride increased the enzymatic activity of α-amylase, while potassium and sodium from their chlorides served as enzyme inhibitors. The Km and Vmax values ​​found were 0.04 mg/mL and 46.95 µmol/min/mL, respectively, indicating that the substrate has affinity for α-amylase. Therefore, the results demonstrate that the residues of C. linearifolius can be used as a substrate for A. niger in the production of enzymatic extracts, such as α-amylase.

scholarly journals Lovastatin Production byAspergillus terreusUsing Agro-Biomass as Substrate in Solid State Fermentation

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Mohammad Faseleh Jahromi ◽  
Juan Boo Liang ◽  
Yin Wan Ho ◽  
Rosfarizan Mohamad ◽  
Yong Meng Goh ◽  
...  
Keyword(s):  
Particle Size ◽  
Solid State ◽  
Moisture Content ◽  
Nitrogen Source ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Initial Moisture Content ◽  
Fermentation Time ◽  
Maximum Production ◽  
Additional Nitrogen

Ability of two strains ofAspergillus terreus(ATCC 74135 and ATCC 20542) for production of lovastatin in solid state fermentation (SSF) using rice straw (RS) and oil palm frond (OPF) was investigated. Results showed that RS is a better substrate for production of lovastatin in SSF. Maximum production of lovastatin has been obtained usingA. terreusATCC 74135 and RS as substrate without additional nitrogen source (157.07 mg/kg dry matter (DM)). Although additional nitrogen source has no benefit effect on enhancing the lovastatin production using RS substrate, it improved the lovastatin production using OPF with maximum production of 70.17 and 63.76 mg/kg DM forA. terreusATCC 20542 andA. terreusATCC 74135, respectively (soybean meal as nitrogen source). Incubation temperature, moisture content, and particle size had shown significant effect on lovastatin production (P<0.01) and inoculums size and pH had no significant effect on lovastatin production (P>0.05). Results also have shown that pH 6, 25°C incubation temperature, 1.4 to 2 mm particle size, 50% initial moisture content, and 8 days fermentation time are the best conditions for lovastatin production in SSF. Maximum production of lovastatin using optimized condition was 175.85 and 260.85 mg/kg DM forA. terreusATCC 20542 and ATCC 74135, respectively, using RS as substrate.

scholarly journals PRODUCTION AND CHARACTERIZATION OF CELLULOLYTIC ENZYMES BY ASPERGILLUS NIGER AND RHIZOPUS SP. BY SOLID STATE FERMENTATION OF PRICKLY PEAR

2016 ◽  
Vol 29 (1) ◽  
pp. 222-233 ◽  
Author(s):  
TAMIRES CARVALHO DOS SANTOS ◽  
GEORGE ABREU FILHO ◽  
AILA RIANY DE BRITO ◽  
AURELIANO JOSÉ VIEIRA PIRES ◽  
RENATA CRISTINA FERREIRA BONOMO ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Water Activity ◽  
Solid State Fermentation ◽  
Cellulase Production ◽  
Cellulolytic Enzymes ◽  
Optimum Conditions ◽  
Thermostable Enzymes ◽  
Fermentation Time ◽  
Rhizopus Sp

ABSTRACT: Prickly palm cactus husk was used as a solid-state fermentation support substrate for the production of cellulolytic enzymes using Aspergillus niger and Rhizopus sp. A Box-Behnken design was used to evaluate the effects of water activity, fermentation time and temperature on endoglucanase and total cellulase production. Response Surface Methodology showed that optimum conditions for endoglucanase production were achieved at after 70.35 h of fermentation at 29.56°C and a water activity of 0.875 for Aspergillus niger and after 68.12 h at 30.41°C for Rhizopus sp. Optimum conditions for total cellulase production were achieved after 74.27 h of fermentation at 31.22°C for Aspergillus niger and after 72.48 h and 27.86°C for Rhizopus sp. Water activity had a significant effect on Aspergillus niger endoglucanase production only. In industrial applications, enzymatic characterization is important for optimizing variables such as temperature and pH. In this study we showed that endoglucanase and total cellulase had a high level of thermostability and pH stability in all the enzymatic extracts. Enzymatic deactivation kinetic experiments indicated that the enzymes remained active after the freezing of the crude extract. Based on the results, bioconversion of cactus is an excellent alternative for the production of thermostable enzymes.

scholarly journals Optimization of productions of cellulolytic enzymes by Aspergillus niger using residue of mango a substrate

2011 ◽  
Vol 41 (12) ◽  
pp. 2210-2216 ◽  
Author(s):  
Tamires Carvalho dos Santos ◽  
Ingrid Souza Cavalcanti ◽  
Renata Cristina Ferreira Bonomo ◽  
Nivio Batista Santana ◽  
Marcelo Franco
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Water Activity ◽  
Solid State Fermentation ◽  
Cellulolytic Enzymes ◽  
Fermentation Time ◽  
Kinetic Activity ◽  
Activity Of Enzymes ◽  
Activity Variable ◽  
Statistical Results

The present paper analyses the effects of water activity (0.88, 0.94 and 0.97) and of fermentation time (24, 48, 72, 96 and 120 hours) on the kinetic activity of enzymes cellulolytic, produced during the solid state fermentation of waste from the improvement of mango, with the aid of fungus species Aspergillus niger. Solid state fermentation was carried out at 35°C inside a bacteriological incubator. The statistical results indicated that the best activity for enzyme CMCase was 7.26U g-1 after 74.51 hours of fermentation, whereas for enzyme FPase was 2.55U g-1 after 98.52 hours, both presenting best results in approximately 0.928 of water activity. Pareto charts have showed that fermentation time has greater effect over the activity of enzyme CMCase, while the water activity variable has greater effect over enzyme FPase activity. During fermentation the fungus synthesized the enzymes without the need of inductors other than mango residue and water.

scholarly journals EFFECT OF FERMENTATION TIME, MOISTURE CONTENT, AND TEMPERATURE ON SORBITOL PRODUCTION VIA SOLID STATE FERMENTATION PROCESS

2017 ◽  
Vol 1 (1) ◽  
pp. 64-71
Author(s):  
Zuriana Sidi Ahmad ◽  
Mimi Sakinah Abdul Munaim
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Product Yield ◽  
High Yield ◽  
Time Range ◽  
Fermentation Time ◽  
Wood Sawdust ◽  
Ssf Process

Malaysia is the largest country that has produced many types of waste. One of it is Meranti wood sawdust. These wastes result in a significant environmental problem if not dispose it in the proper manner. The main objective of this article is to produce the high yield of sorbitol by solid state fermentation (SSF) process from pretreated Meranti wood sawdust using bacterium Lactobacillus plantarum (BAA 793; NCIMB 8826). One factorat a time (OFAT) was studied for further process using solid state fermentation (SSF) process and investigated the effect of relevant parameters (fermentation time, range: 2 hours to 14 hours, moisture content, range: 40% to 90%, temperature, range: 25 oC to 45 oC) to the solid-state fermentation (SSF) process in producing high yield of sorbitol. The highest product yield was obtained at 50% moisture content, at 10 hours of fermentation time and 35 oC of incubation temperature where the concentration of sorbitol was 25.68 g/L respectively. This study also showed that the solid state fermentation (SSF) process will produce the high yield of sorbitol production compared to the submerged fermentation and could serve as a-low cost substrate for bioproducts production especially sorbitol

scholarly journals Produksi β-Glukosidase Aspergillus niger BIO 2173 dengan Fermentasi Padat Menggunakan Substrat Dedak

2018 ◽  
Vol 8 (01) ◽  
pp. 33
Author(s):  
Sri Sugiwati ◽  
Maggy Thenawidjaja Suhartono ◽  
Muhammad Hanafi ◽  
Hanifah Nuryani Lioe
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Water Content ◽  
Incubation Period ◽  
Rice Bran ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Mineral Salts ◽  
Fermentation Conditions ◽  
Fermentation Substrate

Production of β-Glucosidase Aspergillus niger BIO 2173 on Solid State Fermentation Using Rice Bran as SubstrateAbstractβ-Glucosidase (EC 3.2.1.21) is a part of the cellulase enzyme complex which acts synergistically with exoglucanase and endoglucanase to hydrolyze cellulose into glucose. The purpose of this study was to obtain the maximum fermentation conditions for production of b-glucosidase Aspergillus niger BIO 2173 with solid state fermentation using rice bran as fermentation substrate. The factors that affect the production of b-glucosidase which consist of initial pH of the fermentation medium, incubation period, ratio of water content to fermentation substrate, incubation temperature and addition of the Mandel’s mineral salts solution were examined in the study. The results showed that maximum fermentation conditions for β-glucosidase production were at initial of fermentation pH of 2,0, incubation period of 7 days, ratio of water content to substrate of 1:1, and incubation temperature of 32oC. Addition of Mandel’s mineral salts solution to the fermentation substrate at maximum fermentation conditions increased the activity and specific activity of β-glucosidase crude extract up to 5,24 ± 0,57 U/mL and 2,46 ± 0,04 U/mg, respectively.Abstrakβ-Glukosidase (EC 3.2.1.21) merupakan bagian dari enzim multi kompleks selulase, yang bekerja secara sinergis dengan eksoglukanase dan endoglukanase menghidrolisis selulosa menjadi glukosa. Tujuan dari penelitian ini adalah mendapatkan kondisi fermentasi maksimum untuk produksi β-glukosidaseAspergillus niger BIO 2173 dengan fermentasi media padat menggunakan substrat dedak. Pengujian dilakukan terhadap faktor-faktor yang mempengaruhi produksi b-glukosidase, yaitu pH awal medium fermentasi, waktu inkubasi, perbandingan kandungan air terhadap substrat medium fermentasi, suhu inkubasi dan penambahan larutan garam mineral Mandels. Hasil penelitian menunjukkan bahwa kondisi fermentasi maksimum untuk produksi b-glukosidase adalah pada pH awal medium fermentasi 2,0; waktu inkubasi 7 hari, perbandingan kandungan air terhadap substrat medium fermentasi 1:1, dan suhu inkubasi 32oC. Penambahan larutan garam mineral Mandels ke dalam substrat fermentasi pada kondisi fermentasi maksimum menyebabkan peningkatan aktivitas dan aktivitas spesifk ekstrak kasar b-glukosidase masing-masing sebesar 5,24 ± 0,57 U/mL dan 2,46 ± 0,04 U/mg protein. Kata kunci: β-glukosidase, Aspergillus niger, dedak padi, fermentasi padat, ekstrak kasar

scholarly journals Cocoa shell as a substrate for obtaining endoglucanase and xylanase from Aspergillus oryzae ATCC 10124

2020 ◽  
Vol 42 ◽  
pp. e48211
Author(s):  
Nadabe dos Santos Reis ◽  
Ozana Almeida Lessa ◽  
Clissiane Soares Viana Pacheco ◽  
Norma Eliane Pereira ◽  
Glêydison Amarante Soares ◽  
...  
Keyword(s):  
Solid State ◽  
Water Activity ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Distilled Water ◽  
Alternative Substrate ◽  
Fermentation Time ◽  
Box Behnken Design ◽  
Cocoa Shell ◽  
Temperature Water

The objective of this study was to evaluate the use of cocoa shell as an alternative substrate for the production of endoglucanase and xylanase by Aspergillus orizae ATCC 10124 in solid-state fermentation. The fermentation assays were performed using cocoa shells humidified with distilled water. The parameters incubation temperature, water activity and fermentation time were optimized by using a Box-Behnken design. The maximum activities achieved for endoglucanases and xylanases were 0.846 and 0.945 U g-1 at 35°C, with a water activity equal to 0.9 and an incubation time of 96 hours.

scholarly journals Optimization of Protease from Aspergillus Niger under Solid-State Fermentation Utilizing Shrimp Shell Substrate

2021 ◽  
Vol 11 (6) ◽  
pp. 14809-14824
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Proteolytic Enzymes ◽  
Initial Moisture Content ◽  
Inoculum Size ◽  
Protease Production ◽  
Shrimp Shell

Proteases, also known as proteinases or proteolytic enzymes, belong to a group of hydrolases. It can be applied in numerous fields and industries. Solid-state fermentation (SSF) is recognized as an effective method to produce protease. The ultimate aim of this study is to optimize the production of protease from Aspergillus niger under solid-state fermentation (SSF) by utilizing shrimp shell powder as a solid substrate. It was found that the produced protease from SSF was slightly alkaline. The correlation between factors operating parameters (incubation temperature, inoculum size, moisture content) for enzyme production is analyzed using statistical software, Minitab 16. A 23 full factorial experimental design was employed, and the enzyme produced was optimized by the method of desirability function. The optimal conditions for protease production of 3.7 U/mg were 35 °C of incubation temperature, 60% of initial moisture content, and 1.0 inoculum size. It is concluded that SSF protease was successfully produced from Aspergillus niger by utilizing shrimp waste as substrate. Through optimization study, moisture content, the interaction between incubation temperature and moisture content, interaction between moisture content and inoculum size significantly impact protease production.

Studies of Soybean Peptides by Solid-State Fermentation with Multi-Strains

2013 ◽  
Vol 781-784 ◽  
pp. 836-839
Author(s):  
Xiu Li Qin ◽  
Li Hui Zhao
Keyword(s):  
Bacillus Subtilis ◽  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Culture Medium ◽  
Degree Of Hydrolysis ◽  
Fermentation Condition ◽  
Fermentation Time ◽  
Initial Ph ◽  
Hydrolysis Of

In this paper, the condition of aspergillus niger and the bacillus subtilis mixing fermentation to produce soybean peptides was studied. The results indicated that the best fermentation condition of the aspergillus niger and the bacillus subtilis mixing fermentation to produce soybean peptides is that: the initial pH of the culture medium is 8.0, the proportion of mixture strains (aspergillus niger vs bacillus subtilis) is 2 to 1,the fermentation temperature is 30°C and the fermentation time is 80 hours. In this condition the degree of hydrolysis of the fermentation bean pulp is 36.5%.

scholarly journals Optimisation of solid-state fermentation of Aspergillus niger JL-15 for xylanase production and xylooligosaccharides preparation

2011 ◽  
Vol 29 (No. 5) ◽  
pp. 557-567 ◽  
Author(s):  
X.-J. Dai ◽  
M.-Q. Liu ◽  
H.-X. Jin ◽  
M.-Y. Jing
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Xylanase Activity ◽  
Orange Peel ◽  
Basic Medium ◽  
Relative Molecular Mass ◽  
Xylanase Production ◽  
Fermentation Time

The production of xylanase (XylA) by Aspergillus niger JL-15 in solid-state fermentation (SSF) on orange peel was optimised by the response surface methodology (RSM). The results revealed that four factors had significant effects on the XylA production (P &lt; 0.05), that is the concentrations of the added glycerin and ammonium sulfate, the moisture content, and fermentation time. Exploying orange peel as the solid substrate, maximum xylanase activity (917.7&nbsp;U/g dry fermentation product) was obtained at 4.2% glycerin, 3.1% (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>, 61% moisture content, and 73.4-h fermentation, this activity being close to the predicted one and 3.2 times higher than that of the basic medium (218.5&nbsp;U/g). Optimum temperature and pH for XylA activity were 55&deg;C and pH 5.0, respectively. SDS-PAGE analysis showed that the relative molecular mass of XylA was about 30.0 kDa. XylA exhibited K<sub>m</sub> and V<sub>max </sub>values of 9.24 mg/ml and 54.05&nbsp;&mu;mol/min/ml, respectively. XylA liberated mainly xylotriose from birchwood xylan and wheat bran, respectively. XylA was an endo-acting xylanase with transglycosylation activity, with the ability to hydrolyse, xylobiose, xylotriose, xylotetraose, xylopentaose, and xylohexaose.

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