scholarly journals Anxiolytic activity of ferulic acid in the light-dark test in zebrafish

2021 ◽  
Vol 10 (11) ◽  
pp. e582101119894
Author(s):  
Susi Mara Soecki Sborgi ◽  
Lillian Caroline Fernandes ◽  
Anderson Gustavo Santos ◽  
Marcelo Machado Ferro ◽  
Edmar Miyoshi
Keyword(s):  
Depressive Symptoms ◽  
Ferulic Acid ◽  
Mechanism Of Action ◽  
Anxiolytic Effect ◽  
Preference Test ◽  
Positive Control ◽  
Anxiolytic Activity ◽  
Antidepressive Drugs ◽  
Benzodiazepine Binding ◽  
Positive Results

The anxiety disorders belong to a group of mental disorders in which the patients present excessive fear and worry. Studies with ferulic acid have shown positive results on treating depressive symptoms. As many antidepressive drugs are effective in treating anxiety, the objective of the present study was to evaluate ferulic acid’s anxiolytic activity and possible mechanism of action in the light-dark test in zebrafish. To evaluate anxiolytic activity, the light-dark preference test was performed after exposure of the animals to ferulic acid or positive control (clonazepam or fluoxetine). Ferulic acid increased the time spent in the clear compartment at concentrations of 250 and 500 mg/L, not differing from the groups exposed to clonazepam or fluoxetine. To evaluate the possible mechanism of action, pre-exposure to flumazenil was carried out, followed by exposure to ferulic acid or positive control, with subsequent testing. Pre-exposure to flumazenil caused a significant reduction in the time spent in the clear compartment of ferulic acid and clonazepam groups but did not alter the effect of exposure to fluoxetine. These results suggest that ferulic acid promotes an anxiolytic effect, possibly through an action at the benzodiazepine binding site at the GABAA receptor.

scholarly journals Unreliable Measurement of Basophil Maximum Leukotriene Release with the Bühlmann CAST 2000 Enzyme-Linked Immunosorbent Assay Kit

2006 ◽  
Vol 13 (3) ◽  
pp. 420-422 ◽  
Author(s):  
S. E. Burastero ◽  
C. Paolucci ◽  
D. Breda ◽  
G. Monasterolo ◽  
R. E. Rossi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
False Positive ◽  
Positive Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Positive Results

ABSTRACT The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-FcεRI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.

Development and comparative evaluation of immunoblot assays for detecting autoantibodies to Scl 70 and Jo 1 antigens in serum

1990 ◽  
Vol 36 (12) ◽  
pp. 2053-2056 ◽  
Author(s):  
T Fonong ◽  
S M Evans ◽  
H A Homburger
Keyword(s):  
Comparative Evaluation ◽  
Clinical Laboratory ◽  
Connective Tissue Diseases ◽  
Positive Control ◽  
Analytical Sensitivity ◽  
Healthy Controls ◽  
Clinical Use ◽  
Immunoblot Assay ◽  
Negative Results ◽  
Positive Results

Abstract We developed rapid 24-h immunoblot assays for detecting autoantibodies to Scl 70 and Jo 1 antigens in serum. In comparative studies, we evaluated the analytical sensitivity of the immunoblot assays and commercial immunodiffusion assays for anti-Scl 70 and anti-Jo 1 autoantibodies with the use of positive control sera, and compared the frequencies of positive and negative results in a group of 116 sera, including specimens from 34 healthy controls and 82 patients with various connective-tissue diseases. The immunoblot assays were greater than 100-fold more sensitive than immunodiffusion for detecting both autoantibodies. Despite greater analytical sensitivity, there were no false-positive results by the immunoblot assay for anti-Scl 70 or anti-Jo 1 autoantibodies in sera from either the controls or the patients. The diagnostic sensitivity of the immunoblot assay for anti-Scl 70 autoantibodies in patients with scleroderma was greater than that of the immunodiffusion assay, 70% vs 20%, and was equivalent in patients with polymyositis, 43%. We conclude that rapid immunoblot assays for anti-Scl 70 and anti-Jo 1 autoantibodies are superior to immunodiffusion assays for clinical use and are suitable for routine use in the clinical laboratory.

Euphytose®, an association of plant extracts with anxiolytic activity: Investigation of its mechanism of action by anin vitro binding study

1991 ◽  
Vol 5 (6) ◽  
pp. 241-244 ◽  
Author(s):  
Marc Valli ◽  
Monique Paubert-Braquet ◽  
Sylvie Picot ◽  
Richard Fabre ◽  
Gérard Lefrançois ◽  
...  
Keyword(s):  
Mechanism Of Action ◽  
Plant Extracts ◽  
Anxiolytic Activity ◽  
Binding Study ◽  
Vitro Binding

scholarly journals Absence of sibutramine effect on spontaneous anxiety in rats

2010 ◽  
Vol 54 (4) ◽  
pp. 375-380 ◽  
Author(s):  
Silvana S. Frassetto ◽  
Isis O. Alves ◽  
Marislane M. Santos ◽  
Ana E. S. Schmidt ◽  
Janaína J. Lopes ◽  
...  
Keyword(s):  
Chronic Treatment ◽  
Elevated Plus Maze ◽  
Anxiolytic Effect ◽  
Chronic Administration ◽  
Positive Control ◽  
Maximum Effect ◽  
Plus Maze ◽  
The Central Nervous System ◽  
Treatment Of Obesity ◽  
Acute And Chronic Treatments

INTRODUSTION: Sibutramine has been described as a drug recommended for treatment of obesity, since it has the ability to inhibit the reuptake of serotonin and noradrenaline in the central nervous system, thereby increasing energy expenditure. OBJECTIVE: Investigate the anxiogenic and anxiolytic effects of acute and chronic treatment with sibutramine in rats submitted to the task of the elevated plus-maze. METHODS: Diazepam was used as a positive control for the anxiolytic effect, and the task of the elevated plus-maze showed sensitivity to detect the effect. In the chronic treatment, sibutramine was ingested for a period of two months. RESULTS: The acute and chronic treatments at the studied dose, which is described to produce a maximum effect of anti-obesity in rats, did not interfere with anxiety. CONCLUSIONS: The acute and chronic administration of sibutramine is not related to anxiolytic or anxiogenic effects.

scholarly journals Xylazine as a Drug of Abuse and Its Effects on the Generation of Reactive Species and DNA Damage on Human Umbilical Vein Endothelial Cells

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Luz Silva-Torres ◽  
Christian Veléz ◽  
Lyvia Álvarez ◽  
Beatriz Zayas
Keyword(s):  
Dna Damage ◽  
Endothelial Cells ◽  
Dna Fragmentation ◽  
Mechanism Of Action ◽  
Umbilical Vein ◽  
Positive Control ◽  
Potential Health ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells ◽  
Apoptotic Mechanism

Human xylazine (XYL) abuse among addicts has received great interest due to its potential toxic effects upon addicts and the need to understand the mechanism of action associated with the potential health effects. XYL is an alpha-2 agonist restricted to veterinarian applications, without human medical applications. Our previous work demonstrated that XYL and its combination with cocaine (COC) and/or 6-monoacetylmorphine (6-MAM) induce cell death through an apoptotic mechanism. The aim of this study was to determine the effect of xylazine on the generation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) as well as DNA damage on endothelial cell. Human umbilical vein endothelial cells (HUVEC) were treated with XYL (60 μM), COC (160 μM), 6-MAM (160 μM), camptothecin (positive control, 50 μM), XYL/COC (50 μM), XYL/6-MAM (50 μM), and XYL/COC/6-MAM (40 μM) for a period of 24 hours. Generation of intracellular ROS, RNS, and DNA fragmentation were analyzed using a fluorometric assay. Results reveal that XYL and 6-MAM increase levels of ROS; no induction of RNS production was observed. The combination of these drugs shows significant increase in DNA fragmentation in G2/M phase, while XYL, COC, and 6-MAM, without combination, present higher DNA fragmentation in G0/G1 phase. These findings support that these drugs and their combination alter important biochemical events aligned with an apoptotic mechanism of action in HUVEC.

N,N′-Dicyclohexylsulfamide and N,N′-diphenethylsulfamide are anticonvulsant sulfamides with affinity for the benzodiazepine binding site of the GABAA receptor and anxiolytic activity in mice

2012 ◽  
Vol 83 (2) ◽  
pp. 253-259 ◽  
Author(s):  
Cristina Wasowski ◽  
Luciana Gavernet ◽  
Ivana A. Barrios ◽  
Maria L. Villalba ◽  
Valentina Pastore ◽  
...  
Keyword(s):  
Binding Site ◽  
Gabaa Receptor ◽  
Anxiolytic Activity ◽  
Benzodiazepine Binding

scholarly journals Evaluation of antianxiety effect of cinnamaldehyde in swiss albino mice

2019 ◽  
Vol 9 (1) ◽  
pp. 85
Author(s):  
Ritesh Churihar ◽  
Sapna A. More ◽  
Pooja S. Mishra ◽  
Savita Vyas ◽  
Hemant Tanwani
Keyword(s):  
Elevated Plus Maze ◽  
Anxiolytic Effect ◽  
Volatile Oil ◽  
Antioxidant Property ◽  
Anxiolytic Activity ◽  
Protective Effects ◽  
Standard Drug ◽  
Group I ◽  
Plus Maze ◽  
Per Se

Background: Cinnamon is one of the best known spices used as an herbal medicine. Cinnamaldehyde (CNM) the volatile oil, which was present in the essential oil of the bark, is the important constituents of cinnamon. Cinnamon has been investigated for its various effects like peptic ulcer protection, antioxidant property, inhibition of tau aggregation, anti-inflammatory activity, effect on cardiovascular system, anti-nociceptive activity, hepato-protective effects, hypolipidemic and antidiabetic activites. The present study was aimed to evaluate the anxiolytic effect of CNM per se and its interaction with diazepam in swiss albino mice.Methods: Anxiolytic activity was evaluated by elevated plus maze method. A group of 36 healthy mice of either sex weighing 20-30 grams were divided at random into six groups (n=6). CNM and diazepam were dissolved in tween twenty 20% to maintain uniformity of the solvent and given orally. Group I was given twenty 20% (10 ml/kg, p.o.), group II diazepam (0.5 mg/kg, p.o.), group III diazepam (1 mg/kg, p.o.), group IV cinnamaldehyde (100 mg/kg, p.o.), group V cinnamaldehyde (200 mg/kg, p.o.), group VI cinnamaldehyde and diazepam (100 mg/kg and 0.5 mg/kg, p.o.).Results: Cinnamaldehyde per se showed no anxiolytic effect at any dose (p<0.05). The standard drug diazepam has shown significant anxiolytic activity on elevated plus maze. Whereas combination of diazepam 0.5 mg/kg and cinnamaldehyde 100 mg/kg showed significant increase in the time spent in open arms as compared to all groups (p<0.05).Conclusions: CNM per se did not show any effect on anxiety but enhanced the action of diazepam when co-administered.

scholarly journals Sedative and anxiolytic effect of the methanolic extract of Erythrina edulis Triana ex Micheli in mice

Bionatura ◽  
2018 ◽  
Vol 01 (Bionatura Conference Serie) ◽  
Author(s):  
Josué Sarmiento ◽  
Mariana Saa ◽  
Eugenia Peñaherrera
Keyword(s):  
Anxiolytic Effect ◽  
Artemia Salina ◽  
Anxiolytic Activity ◽  
Phytochemical Analysis ◽  
Traditional Use ◽  
Elevated Plus Maze Test ◽  
Sedative Activity ◽  
The Central Nervous System ◽  
Cd1 Mice ◽  
Traction Test

The traditional use of medicinal plants and its link with pharmacological studies is essential in order to corroborate the effect scientifically. The present work was an experimental study designed to investigate the sedative and anxiolytic effect of the methanol extract of the leaves of Erythrina edulis. The extract was prepared by percolation. Different concentrations: 500, 250 and 125 mg/kg of the extract and diazepam as a controlled drug were administered intraperitoneally to Swiss CD1 mice for both, sedation and anxiety tests. The sleep enhancement test with pentobarbital and the traction test were used for the sedative activity. Results showed that the extract did not possess sedative activity in any of the administered doses. However, the possibility of an opposite effect was observed as an activation of the central nervous system probably. The anxiolytic activity was evaluated by the open field test and the elevated plus maze test, where a behavior with a low level of anxiety was observed. Altogether, the results suggest that the extract has an anxiolytic activity without sedation, which supports traditional use. But, its responses are not dose-dependent, and its mechanism of action is unknown. Besides, toxicity assay was performed in Artemia salina nauplii, resulting in a lethal concentration 50 of 16.570 ug/mL corresponding to zero toxicity. Finally, the phytochemical analysis of the extract revealed the presence of flavonoids (flavonols, flavones and phenolic carboxylic acids), anthrones, anthranols, terpenes (mono and sesquiterpenes), saponins and hydrolysable tannins.

Anxiolytic Activity of Apocynum venetum L. and its Proposed Mechanism of Action

Planta Medica ◽  
2008 ◽  
Vol 74 (03) ◽  
Author(s):  
O Grundmann ◽  
J Nakajima ◽  
V Butterweck
Keyword(s):  
Mechanism Of Action ◽  
Anxiolytic Activity ◽  
Apocynum Venetum

scholarly journals Anxiolytic and Antidepressant Effects of the Hydroethanolic Extract from the Leaves of Aloysia polystachya (Griseb.) Moldenke: A Study on Zebrafish (Danio rerio)

2019 ◽  
Vol 12 (3) ◽  
pp. 106 ◽  
Author(s):  
Nayara Costa de Melo ◽  
Brenda Lorena Sánchez-Ortiz ◽  
Tafnis Ingret dos Santos Sampaio ◽  
Arlindo César Matias Pereira ◽  
Fernando Luiz Pinheiro da Silva Neto ◽  
...  
Keyword(s):  
Folk Medicine ◽  
Anxiolytic Effect ◽  
Antidepressant Activity ◽  
Ultra Performance Liquid Chromatography ◽  
Positive Control ◽  
Antidepressant Effect ◽  
Main Compound ◽  
Anxiogenic Effect ◽  
Antidepressant Agent ◽  
Hydroethanolic Extract

Medicinal plants such as Aloysia polystachya are often used in the treatment of psychiatric diseases, including anxiety- and depression-related humor disturbances. In folk medicine, A. polystachya is used to treat digestive and respiratory tract disturbances, as a sedative and antidepressant agent, and as a tonic for the nerves. This study aimed to evaluate the antidepressant and anxiolytic effect from the hydroethanolic extract from the leaves of Aloysia polystachya (HELAp) in zebrafish. The extract was analyzed through ultra-performance liquid chromatography-mass spectroscopy (UPLC-MS) and the main compound detected was acteoside. HELAp was administered orally (10 mg/kg) and through immersion (mg/L). The anxiolytic activity was evaluated through the scototaxis (light–dark) test using caffeine as an anxiogenic agent and buspirone as a positive control. The parameters assessed were: period spent in the white compartment (s), latency (s), alternations (n), erratic swims (n), period of freezing (s), thigmotaxis (s), and risk evaluation (n). The antidepressant effect was evaluated through the novel tank diving test using 1% ethanol, unpredictable chronic stress, and social isolation as depressors; fluoxetine was used as a positive control. The parameters assessed were: period spent at the top of the tank, latency, quadrants crossed, erratic swim, period of freezing, and distance of swam. The main chemical compound of HELAp was acteoside. The administration of the extract on zebrafish managed to revert the anxiogenic effect of caffeine without impairing their locomotion. Additionally, the treatment exerted antidepressant activity similarly to fluoxetine. Overall, the results suggest a significant anxiolytic and antidepressant activity to the extract, which is probably due to the presence of the major compound, acteoside.

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