scholarly journals Estudo In Vitro da limpeza de canais simulados (Wave One Gold x W-File)

2021 ◽  
Vol 10 (13) ◽  
pp. e541101321693
Author(s):  
Marcely Reis Silva ◽  
Adolfo de Matos de Carvalho ◽  
Matheus Almeida Rodrigues ◽  
Edeilton Santana de Oliveira Júnior e Bezerra ◽  
Caio Cesar Souza ◽  
...  
Keyword(s):  
Adobe Photoshop

Objetivo: A limpeza e modelagem do canal radicular são etapas importantes do tratamento endodôntico, e pode influenciar no sucesso final do tratamento. O objetivo deste estudo foi comparar a modelagem dos instrumentos reciprocantes Wave One Gold (WOG – Dentisply, Maillefer, Baillagues, Suiça) e W-File (WF – TDK, Shenzlen superfine Technology Co, China) em canais radiculares simulados. Materiais e Métodos: foram utilizados 20 blocos de acrílico, divididos em dois grupos (n=10): grupo 1 - WOG; grupo 2 – WF. Antes do preparo do canal radicular, a patência dos canais foi verificada com uma lima K #10. Imagens fotográficas do pré e pós-operatório foram obtidas para posterior sobreposição e análise em software de imagem (Adobe Photoshop CS5.1). A análise estatística foi feita utilizando teste T e o teste Mann Whitney. Resultados: mais resina foi removida do lado externo da curva pelo instrumento Wave One Gold em todos os pontos examinados (p < 0.05). Em relação ao transporte do canal, houve diferenças significativas no orifício do canal (p < 0,05). O instrumento Wave One Gold apresentou preparos mais largos. Concluiu-se que tanto o instrumento Wave One Gold, tanto W-File são seguros para uso em canais curvos, promovendo boa limpeza e modelagem, sendo que o WF obteve preparos mais conservadores.

scholarly journals Assessment of Exogenous Pigmentation on Clear Elastomeric Modules: An in vitro Study

2018 ◽  
Vol 8 (1) ◽  
pp. 33-36
Author(s):  
Shaista N Ansari ◽  
Dhananjay Rathod ◽  
G P Rathod ◽  
Gazal Bisht
Keyword(s):  
Methylene Blue ◽  
In Vitro Study ◽  
Vitro Study ◽  
Orthodontic Appliances ◽  
Significant Difference ◽  
Adobe Photoshop ◽  
Group A ◽  
Strong Staining ◽  
Group B

Objective: To assess in vitro changes in the shade of clear elastomeric modules from different manufacturers influenced by exogeneous pigmentation contained in everyday dietary substances.Materials & Method: The specimen comprised of clear elastomeric modules (Group A: Libral, Group B: TP Orthodontics) which were immersed in dietary substances: ketchup, coffee, tea, pepsi & methylene blue. Their hue, saturation and intensity were figured after 72 hours by methods of Adobe photoshop CS3 to mirror the module recoloring seriousness.Result: Significant difference was found between two types of modules analyzed in this study. Ketchup and methylene blue demonstrated higher staining potential compared to other staining media. Besides, there was no significant difference in staining contrast between tea and Pepsi.Conclusion: Ketchup and methylene blue are strong staining media, ought to be avoided by the patients opting for aesthetic orthodontic appliances. Elastomeric modules manufactured by different companies have differing staining potential. 

scholarly journals Molar Buccal Tubes Front and Back Openings Dimensions and Torsional Play

2018 ◽  
Vol 30 (3) ◽  
pp. 32-39
Author(s):  
Hiyam J Al-Zubaidi ◽  
Akram F Alhuwaizi
Keyword(s):  
Orthodontic Treatment ◽  
In Vitro Study ◽  
Free Fall ◽  
Optical Microscope ◽  
The Other ◽  
Posterior Teeth ◽  
Adobe Photoshop ◽  
The Wire ◽  
Metal Block

Background/: Buccal tubes are orthodontic attachments used on the posterior teeth instead of bands, so it is important to focus on the effect of their properties on orthodontic treatment. The aims of the present in vitro study are to evaluate and compare the buccal tube front and back openings dimensions and the torsional play angle of six different brands. Materials and Methods: The samples consisted of Single bondable, non-convertible first molar buccal tubes from six brands supplied from six companies (Dentaurum, Forestadent, Ormco, 3M, American Orthodontic, A-Star). Regarding tube opening dimension, ten buccal tubes of each brand were examined by an optical microscope. Each tube was fixed during examination using synthetic mud and oriented for observation of the front and back slot openings. A picture was taken for both tube openings and the result appeared on the computer's screen where width and height measurements were made. While regarding torsional play angle, ten buccal tubes of each brand were used. Each tube was fixed on a metal block attached to a surveyor base. Then an L-shaped wire was inserted inside the front opening of the tube. Two photographs were taken, one with the wire in free fall position and the other with the wire elevated by a 10g weight with the same angle of shooting as the first photograph. Later, the two images were superimposed in Adobe Photoshop program, and an electronic MB-ruler Software was used to calculate the angle which represents the torsional play within each tube. The data were then statistically analyzed using ANOVA and LSD tests. Results: There are marked differences between measured tube dimensions and the manufacturer stated dimensions with the front tube openings being generally larger than the back opening dimensions. Furthermore, the torsional play angle was highest in A-Star and smallest in Ormco's tubes. This angle was significantly correlated to the height of the tube front opening. Conclusion: It can be concluded that tube dimension varies among different companies and effect greatly torsional play angle.

scholarly journals FIABILIDAD DE UN NUEVO SISTEMA PARA LA DETERMINACIÓN Y ANÁLISIS DEL COLOR DENTAL UTILIZANDO IMAGENOLOGÍA DIGITAL

2019 ◽  
pp. 35-40
Keyword(s):  
Ad Hoc ◽  
Digital Camera ◽  
Digital Photograph ◽  
Clinical Procedures ◽  
Palabras Clave ◽  
Adobe Photoshop ◽  
Para Determinar ◽  
The Continuum ◽  
Shade Analysis

FIABILIDAD DE UN NUEVO SISTEMA PARA LA DETERMINACIÓN Y ANÁLISIS DEL COLOR DENTAL UTILIZANDO IMAGENOLOGÍA DIGITAL Rony Christian Hidalgo-Lostaunau , Sandra Tatiana Córdova-Aliaga, Iván Dante Huamantumba-Espinoza DOI: https://doi.org/10.33017/RevECIPeru2009.0008/ RESUMEN El color dental es apareado con guías de color para determinar una referencia que permita su descripción y comunicación en procedimientos clínicos donde se empleen materiales dentocoloreados. Objetivo: establecer la fiabilidad de un nuevo sistema de registro y análisis del color dental empleando imágenes digitalizadas, un cubículo fotográfico con iluminación ad hoc y un software de edición fotográfica, con la guía de color Vitapan 3D Master. Métodos: fueron fotografiadas con una cámara digital a 5x las 5 primeras cuentas de cada nivel de claridad de la guía Vitapan 3D-M 6 veces sucesivas cada una, sin retirar la cuenta y retirando la cuenta entre toma y toma, empleando un cubículo fotográfico iluminado con fluorescente daylight (2350lm, IRC >95, 5400°K); registradas en formato TIFF, adaptadas en Adobe Photoshop 7.0, para ser analizadas por el histograma del color del sistema CIELab que admite este software resultados: los márgenes de error al realizar tomas continuas es de 1.52 a 3.76, y discontinuas es de 1.50 a 3.78. No se hallaron diferencias significativas al comparar ambos métodos en los 5 niveles de L* (Wilcoxo p≥0.138), Blancura (Wilcoxo p≥0.116), tampoco ∆E superiores a 0.84 Conclusión: la semejanza en el margen de error otorga fiabilidad al método; la validez del sistema por su concordancia en el análisis CIELab de las imágenes digitales de las cuentas de color, permiten sugerirlo sistema como un de alta reproducibilidad para la cuantificación objetiva del color dental in vitro, a ser empleado en la docencia e investigación del color dental y sus implicancias. Palabras clave: análisis del color, estudio de validación, fiabilidad, CIELab, fotografía digital, imagen digital. ABSTRACT The dental color is matched up with color guides to determine a reference that allows its description and communication in clinical procedures where tooth-colored materials are used. OBJECTIVE: To establish the reliability of a new system for registration and analysis of the dental color using digitized images, a photographic cubicle with illumination ad hoc and a software of photographic edition, with the color guide Vitapan 3D Master. METHODS: The first 5 bills of each level of clarity of the guide Vitapan 3D-M were photographed with a digital camera at 5x, in 6 successive times each a, without moving away the bead and moving away the bead among picture and picture, using an illuminated photographic cubicle with fluorescent daylight (2350lm, IRC >95, 5400°K); registered in format TIFF, adapted in Adobe Photoshop 7.0, to be analyzed by the histogram of the color of the system CIELab that admits this software. RESULTS: The error margins to the Continuum takeovers is of 1.52 to 3.76 3.76, and discontinuous it becomes of 1.50 to 3.78. Does it not find significant differences to the comparing both methods in L* levels (Wilcoxo p≥0.138), Whitenig (Wilcoxo p≥0.116), neither ∆E superior to 0.84. CONCLUSION: The similarity in the error margin grants reliability to the method; The validity of the system for his concordance in the analysis CIELab of the digital images of the color beads, allows to suggest it as a reliable instrument of high reproducibility in order to the dental color's objective quantification in vitro, to be employee in the teaching and investigation of the dental color and his sequels. Keywords: shade analysis, Validation Studies, Reliability, CIELab, digital photograph, digital imaging.

scholarly journals The influence of elastomeric ligatures pigmentation on smile aesthetics during orthodontic treatment

2021 ◽  
Vol 26 (2) ◽  
Author(s):  
Amanda Galindo Florêncio MIRANDA ◽  
Ana Paula Terossi de GODOI ◽  
Carolina Carmo de MENEZES ◽  
Mário VEDOVELLO FILHO ◽  
Giovana Cherubini VENEZIAN
Keyword(s):  
Orthodontic Treatment ◽  
Red Wine ◽  
Artificial Saliva ◽  
Control Group ◽  
Satisfaction Scale ◽  
Level Of Satisfaction ◽  
Adobe Photoshop ◽  
Color Data ◽  
Smile Aesthetics

ABSTRACT Aim: To assess the influence of elastomeric ligatures, subjected to a previous in vitro pigmentation process using different substances, on smile aesthetics during orthodontic treatment, from the perception of students and professionals. Methods: Eight elastomeric ligatures of five commercial brands (3M/Unitek, American Orthodontics, Morelli, Ortho Technology, and Orthometric) (n=8) were immersed in coffee, Coca-Cola, and red wine for one minute per day, for 28 days; and another group of ligatures was immersed in artificial saliva. All samples were photographed and subsequently analyzed using the Adobe Photoshop software, by the RGB method. Afterwards, the pigmented ligatures were inserted in a patient wearing orthodontic brackets, and zoomed photographs of the smile were taken and presented to 40 evaluators, who filled in a satisfaction scale sheet to express their opinion on the smile aesthetics of each photograph. The color data were subjected to analysis of variance (ANOVA) and Tukey tests. Results: The substance with the highest pigmentation potential was coffee (p< 0.05) followed by red wine (p< 0.05). Comparison among the brands used in this study showed that American Orthodontics and Orthometric had the lowest degree of pigmentation when immersed in coffee and red wine (p< 0.05), respectively. However, the brand that showed the highest level of satisfaction among the evaluators was Ortho Technology. Conclusions: The presence of pigmented elastomeric ligatures affected smile aesthetics, when compared with the control group.

Ultrastructural Investigations of the Contractile Filaments of Amoebae

1974 ◽  
Vol 32 ◽  
pp. 188-189
Author(s):  
P.L. Moore
Keyword(s):  
Cytoplasmic Streaming ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Amoeboid Movement ◽  
Different Types ◽  
Chemical Conditions ◽  
Complete Interpretation

Previous freeze fracture results on the intact giant, amoeba Chaos carolinensis indicated the presence of a fibrillar arrangement of filaments within the cytoplasm. A complete interpretation of the three dimensional ultrastructure of these structures, and their possible role in amoeboid movement was not possible, since comparable results could not be obtained with conventional fixation of intact amoebae. Progress in interpreting the freeze fracture images of amoebae required a more thorough understanding of the different types of filaments present in amoebae, and of the ways in which they could be organized while remaining functional.The recent development of a calcium sensitive, demembranated, amoeboid model of Chaos carolinensis has made it possible to achieve a better understanding of such functional arrangements of amoeboid filaments. In these models the motility of demembranated cytoplasm can be controlled in vitro, and the chemical conditions necessary for contractility, and cytoplasmic streaming can be investigated. It is clear from these studies that “fibrils” exist in amoeboid models, and that they are capable of contracting along their length under conditions similar to those which cause contraction in vertebrate muscles.

In Vitro Induction of Crystals of MT Protein by Vinblastine-Colcemid in Mouse Spermatids

1974 ◽  
Vol 32 ◽  
pp. 132-133
Author(s):  
John J. Wolosewick ◽  
John H. D. Bryan
Keyword(s):  
Endoplasmic Reticulum ◽  
Smooth Endoplasmic Reticulum ◽  
Nuclear Ring ◽  
Rough Er ◽  
Distal Direction ◽  
In Vitro Induction

Early in spermiogenesis the manchette is rapidly assembled in a distal direction from the nuclear-ring-densities. The association of vesicles of smooth endoplasmic reticulum (SER) and the manchette microtubules (MTS) has been reported. In the mouse, osmophilic densities at the distal ends of the manchette are the organizing centers (MTOCS), and are associated with the SER. Rapid MT assembly and the lack of rough ER suggests that there is an existing pool of MT protein. Colcemid potentiates the reaction of vinblastine with tubulin and was used in this investigation to detect this protein.

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

Immunoenzymatic demonstration of the simultaneous presence of transferrin, hemopexin and albumin in a same hepatocyte and their ultrastructural localization

1978 ◽  
Vol 36 (2) ◽  
pp. 88-89
Author(s):  
M. Kraemer ◽  
J. Foucrier ◽  
J. Vassy ◽  
M.T. Chalumeau
Keyword(s):  
Plasma Proteins ◽  
Rat Hepatocytes ◽  
Ultrastructural Localization ◽  
Carrier Proteins ◽  
Normal Cells ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Monospecific Antisera ◽  
Different Levels

Some authors using immunofluorescent techniques had already suggested that some hepatocytes are able to synthetize several plasma proteins. In vitro studies on normal cells or on cells issued of murine hepatomas raise the same conclusion. These works could be indications of an hepatocyte functionnal non-specialization, meanwhile the authors never give direct topographic proofs suitable with this hypothesis.The use of immunoenzymatic techniques after obtention of monospecific antisera had seemed to us useful to bring forward a better knowledge of this problem. We have studied three carrier proteins (transferrin = Tf, hemopexin = Hx, albumin = Alb) operating at different levels in iron metabolism by demonstrating and localizing the adult rat hepatocytes involved in their synthesis.Immunological, histological and ultrastructural methods have been described in a previous work.

Sem Studies of Co-Cr-Mo Surgical Implant Alloy Corrosion Behavior

1982 ◽  
Vol 40 ◽  
pp. 520-521
Author(s):  
Ann Chidester Van Orden ◽  
John L. Chidester ◽  
Anna C. Fraker ◽  
Pei Sung
Keyword(s):  
Electron Microscopy ◽  
Corrosion Behavior ◽  
Anodic Polarization ◽  
Saline Solution ◽  
Saturated Calomel Electrode ◽  
Physiological Saline ◽  
X Ray ◽  
Physiological Saline Solution ◽  
Scanning Electron

The influence of small variations in the composition on the corrosion behavior of Co-Cr-Mo alloys has been studied using scanning electron microscopy (SEM), energy dispersive x-ray analysis (EDX), and electrochemical measurements. SEM and EDX data were correlated with data from in vitro corrosion measurements involving repassivation and also potentiostatic anodic polarization measurements. Specimens studied included the four alloys shown in Table 1. Corrosion tests were conducted in Hanks' physiological saline solution which has a pH of 7.4 and was held at a temperature of 37°C. Specimens were mechanically polished to a surface finish with 0.05 µm A1203, then exposed to the solution and anodically polarized at a rate of 0.006 v/min. All voltages were measured vs. the saturated calomel electrode (s.c.e.).. Specimens had breakdown potentials near 0.47V vs. s.c.e.

Ultrastructural Correlations between Clonal Human Tumor Colonies and in Vivo Neoplasms

1982 ◽  
Vol 40 ◽  
pp. 210-211
Author(s):  
M.J. Murphy ◽  
R.R. Price ◽  
J.C. Sloman
Keyword(s):  
Cultured Cells ◽  
Human Tumor ◽  
Cell Type ◽  
Tumor Mass ◽  
Initial Cell ◽  
Cloning Assay ◽  
Human Tumor Cloning ◽  
The Relationship

The in vitro human tumor cloning assay originally described by Salmon and Hamburger has been applied recently to the investigation of differential anti-tumor drug sensitivities over a broad range of human neoplasms. A major problem in the acceptance of this technique has been the question of the relationship between the cultured cells and the original patient tumor, i.e., whether the colonies that develop derive from the neoplasm or from some other cell type within the initial cell population. A study of the ultrastructural morphology of the cultured cells vs. patient tumor has therefore been undertaken to resolve this question. Direct correlation was assured by division of a common tumor mass at surgical resection, one biopsy being fixed for TEM studies, the second being rapidly transported to the laboratory for culture.

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