scholarly journals Reduction in Enteroccocus faecalis counts produced by three file systems in severely curved canals

2021 ◽  
Vol 10 (2) ◽  
pp. e58910212956
Author(s):  
Wayne Martins Nascimento ◽  
Ana Grasiela da Silva Limoeiro ◽  
Mariana Montagner Moraes ◽  
Danilo De Luca Campos ◽  
João Paulo Drumond ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
File Systems ◽  
Wilcoxon Test ◽  
Control Group ◽  
Root Canal Preparation ◽  
Bacterial Reduction ◽  
Root Canals ◽  
Analysis Methodology ◽  
Level Of Significance ◽  
Microbial Samples

Aim: This study evaluated the effectiveness of the mechanical reduction of intracanal bacteria produced by the endodontic systems Reciproc Blue (VDW GmbH), XP-Endo Shaper (FKG Dentaire) and ProTaper Next (Dentsply Sirona Endodontics) in severely curved canals by culture analysis. Methodology: Fifty severely curved mesiobuccal canals of mandibular molars were selected and instrumented. Following this, six specimens were selected as control, while 44 canals were contaminated with Enterococcus faecalis strains (ATCC 29212). Specimens were incubated for 21 days at 37 ºC. Then, contaminated specimens were randomly divided into 3 groups (n=14): ProTaper Next (G1), XP-Endo Shaper (G2) and Reciproc Blue (G3). Control Group: Six non-contaminated canals were prepared with one of the 3 file systems (n=2). Microbial samples were obtained before (S1) and after root canal preparation (S2). Two roots were observed using scanning electron microscopy to verify biofilm formation.  Quantitative culture analyses of intracanal E. faecalis reduction was performed using CFUs, and the Wilcoxon test was used for intragroup analysis, and intergroup analysis was performed using Kruskal-Wallis test. The level of significance for all analyses was set at P < .05. Results: All systems presented effective bacterial reduction (p<0.05), but still had bacterial growth. ProTaper Next displayed the highest E. faecalis reduction (P<0.05), while XP-Endo Shaper and Reciproc Blue systems presented similar results (P>0.05). ProTaper Next showed the greatest antibacterial action when compared to the XP-Endo Shaper and Reciproc Blue systems. Conclusion: No instrumentation system rendered root canals completely free from bacteria.

scholarly journals Antibacterial Efficacy of Synthetic and Natural-Derived Novel Endodontic Irrigant Solutions

2018 ◽  
Vol 29 (5) ◽  
pp. 459-464 ◽  
Author(s):  
Larissa Tais Soligo ◽  
Ediléia Lodi ◽  
Ana Paula Farina ◽  
Matheus Albino Souza ◽  
Cristina de Mattos Pimenta Vidal ◽  
...  
Keyword(s):  
Sodium Chloride ◽  
Enterococcus Faecalis ◽  
Grape Seed ◽  
Control Group ◽  
Bacterial Reduction ◽  
Calcium Hypochlorite ◽  
Root Canals ◽  
Colony Forming Units ◽  
Before And After ◽  
Post Hoc

Abstract The aim of this study was to compare the efficacy of grape seed extract (GSE), calcium hypochlorite [Ca(ClO)2], and sodium hypochlorite (NaOCl) irrigant solutions with rotary or reciprocating instrumentation for disinfection of root canals inoculated with Enterococcus faecalis. The mesiobuccal root canals of mandibular molars were prepared and inoculated with Enterococcus faecalis for 21 days. The roots were then randomly divided into the following eight experimental groups (n=11) according to the instrumentation technique and disinfection protocol: ProTaper Next or Reciproc R25 with sodium chloride (control group), 6% NaOCl, 6% Ca(ClO)2, or 50% GSE used for irrigation during instrumentation. The antimicrobial activity was determined on the basis of a reduction in colony-forming units (CFUs) counted on bacterial samples collected before and after root canal instrumentation and expressed as a percentage of reduction. Data were evaluated by two-way ANOVA followed by Tukey HSD post-hoc tests (p<0.05). No significant differences were observed in bacterial reduction between the ProTaper Next and Reciproc R25 systems (p>0.05), regardless of the irrigant solution used. Furthermore, all active solutions (6% NaOCl, 50% GSE, and 6% Ca(ClO)2) showed similar potential to reduce bacterial counts (p>0.05) and were significantly more effective than sodium chloride (control) (p<0.05). The results suggest that the GSE and Ca(ClO)2 have potential clinical application as irrigant solutions in endodontic therapy since they present bactericidal efficacy against Enterococcus faecalis.

scholarly journals Comparison of Canal Transportation and Centering Ability of ProGlider and WaveOne Gold Glider in Curved Canals

2020 ◽  
Vol 14 (04) ◽  
pp. 639-643
Author(s):  
Gabriela Behne Miró ◽  
Flávia Sens Fagundes Tomazinho ◽  
Edson Pelisser ◽  
Mariana Maciel Batista Borges ◽  
Marco Antônio Hungaro Duarte ◽  
...  
Keyword(s):  
Root Canal ◽  
File Systems ◽  
Root Canal Preparation ◽  
Volume Increase ◽  
Volume Variation ◽  
Glide Path ◽  
Significant Difference ◽  
Before And After ◽  
Canal Transportation ◽  
Level Of Significance

Abstract Objectives The aim of this study was to compare the volume variation and maintenance of the root canal position when using the ProGlider 16.02 (PG) and the WaveOne Gold Glider 15.02 (WOGG) file systems for glide path preparation. Materials and Methods Twenty-four moderately curved mesiobuccal canals of maxil-lary first molars were selected and randomly divided into two groups: PG and WOGG. The selected teeth were scanned using microtomography before and after root canal preparation to assess centralization and linear transport at 1, 3, 5, and 7 mm from the apical foramen. Statistical Analysis The data were analyzed with the Mann–Whitney U test. The level of significance was 5%. Results There were no significant differences in volume variation or root canal transport (p > 0.05). There was a significant difference in the centralization of the root canal at 3 mm from the foramen (p < 0.05). Conclusions WOGG and PG instruments presented similar results regarding the root canal volume increase and transport. WOGG caused higher decentralization at 3 mm from the apical foramen.

scholarly journals Evaluation of the WaveOne Gold and One Shape New Generation in Reducing Enterococcus faecalis from Root Canal

2018 ◽  
Vol 29 (3) ◽  
pp. 249-253 ◽  
Author(s):  
Raquel Esmeralda Guillén ◽  
Cleber Keiti Nabeshima ◽  
Hector Caballero-Flores ◽  
Miguel Roig Cayón ◽  
Montse Mercadé ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
File Systems ◽  
Bacterial Reduction ◽  
Root Canals ◽  
Single File ◽  
Bacterial Removal ◽  
Significant Difference ◽  
Post Hoc ◽  
New Generation

Abstract The aim of this study was to evaluate the WaveOne Gold and One Shape New Generation systems regarding the bacterial removal from root canals infected with Enterococcus faecalis by comparing them to the conventional WaveOne and One Shape systems. Forty-eight distobuccal root canals of maxillary molars sterilized with ethylene oxide were infected with E. faecalis for 21 days, and then root canal initial bacterial sample was collected with paper cones and plated on M-enterococcus agar. The specimens were randomly divided into 4 groups according to the instrumentation: WaveOne Gold, One Shape New Generation, WaveOne and One Shape. After instrumentation, samples were collected with use of scraping and paper cones at immediate and 7 days after instrumentation. The bacterial reduction was calculated and then made intragroup analysis by Friedman test and intergroup analysis by Kruskal-Wallis with Dunn’s post-hoc test, all at 5% significance. All techniques significantly reduced the number of bacteria in the root canal (p<0.05). WaveOne Gold and One Shape New Generation promoted higher bacterial reduction than WaveOne and One Shape systems (p<0.05), but no significant difference was found between WaveOne Gold and One Shape New Generation or between WaveOne and One Shape (p>0.05). Novel single-file systems promote better bacterial removal than the conventional single-file systems.

scholarly journals Fracture strength of roots instrumented with three different single file systems in curved root canals

2015 ◽  
Vol 09 (02) ◽  
pp. 189-193 ◽  
Author(s):  
Bilge Gulsum Nur ◽  
Evren Ok ◽  
Mustafa Altunsoy ◽  
Mehmet Tanriver ◽  
Ismail Davut Capar
Keyword(s):  
Fracture Strength ◽  
File Systems ◽  
Fracture Load ◽  
Control Group ◽  
Root Canals ◽  
Maxillary Molars ◽  
Single File ◽  
Maxillary Molar ◽  
Molar Teeth ◽  
The Mean

ABSTRACT Objective: The aim of this study was to compare the fracture strength of roots instrumented with three different single file rotary systems in curved mesial root canals of maxillary molars. Materials and Methods: Curvatures of 25°–35° on mesial roots of 60 maxillary molar teeth were sectioned below the cementoenamel junction to obtain roots 11 mm in length. The roots were balanced with respect to buccolingual and mesiodistal diameter and weight. They were distributed into three experimental groups and one control group (no instrumentation) (n = 15): Reciproc rotary file (R25, VDW, Munich, Germany), WaveOne Primary rotary file (Dentsply Tulsa Dental Specialties, Tulsa, UK) and OneShape (Micro-Mega, Besancon, France) rotary file. Vertical load was applied until fracture occurred. Data were statistically analyzed using one-way analysis of variance test (P < 0.05). Results: The mean fracture load was 412 ± 72 Newton (N) for the control group, 395 ± 69 N for the Reciproc group, 373 ± 63 N for the WaveOne group and 332 ± 68 N for the OneShape group. The fracture load differences among three experimental groups were not statistically significant (P > 0.05.) Whereas, the fracture loads of control and OneShape groups were significantly different (P = 0.012). Conclusions: Fracture resistance of the roots instrumented with WaveOne and Reciproc file systems were similar to the control group whereas it was observed that OneShape rotary file systems enhance the fracture strength of standardized curved roots when compared with the control group.

scholarly journals Verification of remaining filling materials in flattened/oval-shaped root canals after use of several endodontic retreatment protocols

RSBO ◽  
2021 ◽  
Vol 17 (2) ◽  
pp. 72-78
Author(s):  
Fábio Carmona Tirintan ◽  
Álvaro Henrique Borges ◽  
Jesus Djalma Pécora ◽  
Manoel dos Santos da Silva Neto ◽  
Matheus Gehrke Barbosa ◽  
...  
Keyword(s):  
Complete Removal ◽  
Lower Amount ◽  
Control Group ◽  
Filling Material ◽  
Root Canal Filling ◽  
Root Canals ◽  
Filling Materials ◽  
Level Of Significance ◽  
Relative Differences ◽  
Endodontic Retreatment

This study verified of remaining filling materials in flattened/oval-shaped root canals after use of several endodontic retreatment protocols. Material and methods: Seventy human mandibular incisors were selected. Sixty specimens received crown-apex preparation performed by ProTaper Next® system andwere filled with hybrid Tagger technique with gutta-percha and AH Plus®. Ten specimens were left unprepared (Control Group). The specimens were randomly distributed in six groups, according the different retreatment protocols: G1 – Clearsonic® + ProTaper Universal Retreatment® + Reciproc®; G2 – Reciproc®; G3 – Clearsonic® + ProTaper Universal Retreatment®; G4 – Clearsonic® + Reciproc®; G5 – Oscillatory EndoEze® + Manual files; G6 – ProTaper Universal Retreatment®. Presence of root canal filling material residual were evaluated using Scanning Electron Microscopy. The relative differences of remaining filling materials between the groups and root thirds were determined by Kolmogorov-Smirnov and Levene tests followed by Tukey post hoc test. The level of significance was set at α = 5%. Results: Regarding the presence residual, G1 (Clearsonic® + ProTaper Universal Retreatment® + Reciproc®), G2 (Reciproc®), G3(Clearsonic® + ProTaper Universal Retreatment®) and G6 (ProTaper Universal Retreatment®) protocols presented a lower amount (p<0.05). The highest values (p<0.05) of remaining filling materials were presented in the G5 (Oscillatory EndoEze® + Manual files) protocol. Intermediate values (p<0.05) were presented by G4 (Clearsonic® + Reciproc®) protocol. Conclusion: The endodontic retreatment protocols no showed effectiveness for complete removal of remaining sealing material from flattened/oval-shaped root canals. 

scholarly journals The influence of working length on the reduction of biofilm and planktonic bacteria in oval canals with reciprocating instrumentation

2021 ◽  
Vol 10 (5) ◽  
pp. e9810514765
Author(s):  
Arieth Cristina Sacomani ◽  
Fernanda Tessaro Cintra ◽  
Adriana de Jesus Soares ◽  
Marcos Frozoni
Keyword(s):  
Bacterial Growth ◽  
Root Canal ◽  
Negative Control ◽  
Bacterial Biofilm ◽  
Control Group ◽  
Root Canal Preparation ◽  
Root Canals ◽  
Planktonic Bacteria ◽  
Working Length ◽  
Significant Difference

To evaluate the influence of reciprocating single-file instrumentation with different working lengths (WL) on the reduction of planktonic bacteria and bacterial biofilm in Enterococcus faecalis-contaminated oval root canals. Methodology: Fifty-five human single-rooted canines were used. Fifty were inoculated with E. faecalis for 21 days for biofilm formation. To confirm the formation of biofilm adhered to the root canal wall, 5 contaminated samples from positive control group were analyzed by SEM. Samples were assigned into 3 groups (n = 15) according to working length determined, G+1 root canal preparation 1 mm beyond the apical foramen, G0 root canal preparation at the major foramen, and G-1 root canal preparation 1 mm short of the major foramen. Five roots were not inoculated to serve as a negative control. Bacteriological samples were collected prior to preparation, initial collection (S1), and after reciprocating instrumentation (S2) by disaggregating biofilm to quantify the reduction of planktonic bacteria and intracanal biofilm at different WL. Bacterial quantitation was performed using colony-forming units per milliliter (CFU / mL) count. Statistical analysis was performed at the significance level of 0.05. Results: No bacterial growth was observed in the negative control. All positive controls demonstrated bacterial growth; S1 from all teeth were positive for bacteria with no significant difference. The post-hoc analysis showed G+1 promoting a significantly higher disinfection than G-1 (p<0,05) and G-1 similar disinfection to G0 (P=962). Conclusion: Instrumentation as close as possible to major foramen or beyond it improves decontamination in oval root canals with reciprocating instrumentation.

scholarly journals A microcomputed tomography evaluation of the shaping ability of three thermally-treated nickel-titanium rotary file systems in curved canals

2018 ◽  
Vol 47 (1) ◽  
pp. 325-334 ◽  
Author(s):  
Zhuwei Huang ◽  
Jingjing Quan ◽  
Jia Liu ◽  
Wen Zhang ◽  
Xiaolei Zhang ◽  
...  
Keyword(s):  
Root Canal ◽  
File Systems ◽  
Microcomputed Tomography ◽  
Nickel Titanium ◽  
Root Canal Preparation ◽  
Root Canals ◽  
Before And After ◽  
Shaping Ability ◽  
Thermally Treated

Objective To evaluate the shaping ability of three thermally-treated rotary nickel-titanium (NiTi) systems including ProTaper Next (PTN), HyFlex™ CM (HFCM) and HyFlex™ EDM (HFEDM) during root canal preparation in simulated root canals. Methods A total of 45 simulated root canals were divided into three groups ( n = 15) and prepared with PTN, HFCM or HFEDM files up to size 25. Microcomputed tomography (microCT) was used to scan the specimens before and after instrumentation. Volume and diameter changes, transportations and centring ratios at 11 levels of the simulated root canals were measured and compared. Results HFEDM caused significantly greater volume increases than HFCM and PTN in the entire root canal and in the apical and middle thirds. HFCM removed the least amount of resin in the coronal third compared with HFEDM and PTN. Overall, HFCM caused significantly less transportation in the apical 2 mm and was better centred than PTN in the apical 3 mm. Conclusion Under the conditions of this study, all systems prepared curved canals without significant shaping errors and instrument fracture. PTN and HFCM cut less resin than HFEDM. HFCM stayed centred apically and cut the least material coronally.

scholarly journals Bacterial removal in flattened root canals with different tapers of ProDesign Logic single-file system

2021 ◽  
Vol 10 (9) ◽  
pp. e5910917701
Author(s):  
Vanessa Sandini ◽  
Viviane Godoy ◽  
Marina Carvalho Prado ◽  
Ricardo Ferreira ◽  
Adriana De-Jesus-Soares ◽  
...  
Keyword(s):  
Bacterial Biofilm ◽  
Bacterial Reduction ◽  
Sterile Saline ◽  
Root Canals ◽  
Bacterial Removal ◽  
Colony Forming Units ◽  
Significant Difference ◽  
Before And After ◽  
Level Of Significance ◽  
Post Hoc

Objective: This study evaluated the bacterial removal in flattened root canals after preparation with different tapers of ProDesign Logic instruments. Methodology: Fifty-five mandibular incisors with flattened root canals were contaminated with a pure culture of Enterococcus faecalis ATCC 29212 for 21 days at 37ºC. Root canals preparation was performed using ProDesign Logic and 0.9% sterile saline solution for irrigation. The specimens were randomly allocated to three experimental groups (n = 15) according to the taper of the instrument used: 25.03, 25.04, or 25.06. Intracanal bacteriological samples were taken before (S1) and after (S2) preparation and the number of colony-forming units per milliliter (CFU/mL) was calculated. Differences in bacterial counts before and after treatment, within each group, were analyzed by the paired t-test. Groups were compared by ANOVA with a post hoc test. The level of significance was set at p <0.05. Results: Mature biofilm formation was confirmed at root canal walls by scanning electron microscopy. Bacterial colonies were significantly reduced in all experimental groups at S2 compared to S1 (p <0.001). No significant difference was observed between groups regarding the percentage of bacterial reduction (p >0.05). Conclusion: Preparation of flattened root canals using ProDesign Logic instruments with tapers of 0.03, 0.04 and 0.06 resulted in similar planktonic and bacterial biofilm reduction. None of the instruments rendered root canals completely free of bacteria.

scholarly journals Histological evaluation of the cleaning effectiveness of two reciprocating single-file systems in severely curved root canals: Reciproc versus WaveOne

2015 ◽  
Vol 09 (01) ◽  
pp. 080-086 ◽  
Author(s):  
Maira de Souza Carvalho ◽  
Emílio Carlos Sponchiado ◽  
Angela Delfina Bitencourt Garrido ◽  
Lucas da Fonseca Roberti Garcia ◽  
André Augusto Franco Marques
Keyword(s):  
File Systems ◽  
Negative Control Group ◽  
Negative Control ◽  
Histological Evaluation ◽  
Control Group ◽  
Root Canals ◽  
Single File ◽  
Significant Difference ◽  
Digital Microscope ◽  
Curved Root Canals

ABSTRACT Objective: The aim of this study was to evaluate the cleaning effectiveness achieved with two reciprocating single-file systems in severely curved root canals: Reciproc and WaveOne. Materials and Methods: Twenty-five mesial roots of mandibular molars were randomly separated into two groups, according to the instrumentation system used. The negative control group consisted of five specimens that were not instrumented. The mesial canals (buccal and lingual) in Reciproc Group were instrumented with file R25 and the WaveOne group with the Primary file. The samples were submitted to histological processing and analyzed under a digital microscope. Results: The WaveOne group presented a larger amount of debris than the Reciproc Group, however, without statistically significant difference (P > 0.05). A larger amount of debris in the control group was observed, with statistically significant difference to Reciproc and WaveOne groups (P < 0.05). Conclusions: The two reciprocating single-file instrumentation systems presented similar effectiveness for root canal cleaning.

scholarly journals Instrument Design May Influence Bacterial Reduction During Root Canal Preparation

2017 ◽  
Vol 28 (5) ◽  
pp. 587-591 ◽  
Author(s):  
Manoel Eduardo de Lima Machado ◽  
Cleber Keiti Nabeshima ◽  
Hector Caballero-Flores ◽  
Moyzés Elmadjian-Filho ◽  
Marco Antônio Húngaro Duarte ◽  
...  
Keyword(s):  
Ethylene Oxide ◽  
Root Canal ◽  
Instrument Design ◽  
Broth Culture ◽  
Bacterial Number ◽  
Root Canal Preparation ◽  
Bacterial Reduction ◽  
Root Canals ◽  
Maxillary Molars ◽  
Paired T Test

Abstract The aim of this study was to evaluate the bacterial reduction promoted by ProTaper Next and Twisted File by comparing to ProTaper Universal and manual technique. Sixty distobuccal root canals of maxillary molars sterilized with ethylene oxide were contaminated with Enterococcus faecalis broth culture. After incubation for 21 days, bacterial samples were collected and cultured on m-Enterococcus agar plates. The root canals were divided into 4 groups, according to the system used for instrumentation: ProTaper Next, Twisted File, ProTaper Universal, and crown down manual technique. Other 8 uncontaminated root canals were control asepsis. Bacterial samples were collected immediately and 7 days after instrumentation. The bacterial reduction was calculated and then made intragroup analysis by paired t-test and intergroup analysis by ANOVA and Tukey tests, all at 5% significance. All techniques significantly reduced the bacterial number in the root canal (p<0.05). ProTaper Next and Twisted File resulted in more bacterial reduction than ProTaper Universal and manual technique (p<0.05). ProTaper Next and Twisted File were similar (p>0.05). It can be concluded that ProTaper Next and Twisted File promote a higher bacterial reduction than Protaper Universal and manual technique.

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