scholarly journals Larval Gnathostomes and Spargana in Chinese Edible Frogs, Hoplobatrachus rugulosus, from Myanmar: Potential Risk of Human Infection

2020 ◽  
Vol 58 (4) ◽  
pp. 467-473
Author(s):  
Jong-Yil Chai ◽  
Bong-Kwang Jung ◽  
Jin-Youp Ryu ◽  
Hyun-Seung Kim ◽  
Sung-Jong Hong ◽  
...  
Keyword(s):  
Potential Risk ◽  
Human Infection ◽  
Local Market ◽  
Digestion Method ◽  
Artificial Digestion ◽  
Potential Risks ◽  
The Mean

Chinese edible frogs, Hoplobatrachus rugulosus, were examined to estimate the potential risks of human gnathostomiasis and sparganosis in Myanmar. A total of 20 frogs were purchased in a local market of Yangon and examined with naked eyes and the artificial digestion method after skin peeling in June 2018 and June 2019. Larvae of gnathostomes and Spirometra (=spargana) were detected in 15 (75.0%) and 15 (75.0%) frogs with average intensities of 10.5 and 6.3 larvae per infected frog, respectively. Gnathostome larvae were 2.75-3.80 (av. 3.30) mm long and 0.29-0.36 (0.33) mm wide. They had a characteristic head bulb with 4 rows of hooklets, a muscular long esophagus, and 2 pairs of cervical sac. The mean number of hooklets were 41, 44, 47, and 50 on the 1st, 2nd, 3rd, and 4th row, respectively. Collected spargana were actively moving, particularly with the scolex part, and have ivory-white color and variable in size. Conclusively, it has been first confirmed that Chinese edible frogs, H. rugulosus, are highly infected with larval gnathostomes and spargana in this study. Consuming these frogs is considered a potential risk of human gnathostomiasis and sparganosis in Myanmar.

scholarly journals Larval Gnathostomes and Zoonotic Trematode Metacercariae in Fish from a Local Market in Yangon City, Myanmar

2020 ◽  
Vol 58 (6) ◽  
pp. 701-707
Author(s):  
Jong-Yil Chai ◽  
Bong-Kwang Jung ◽  
Keon Hoon Lee ◽  
Jin-Youp Ryu ◽  
Hyeon-Seung Kim ◽  
...  
Keyword(s):  
Fish Species ◽  
Opisthorchis Viverrini ◽  
Trematode Species ◽  
Local Market ◽  
Infection Status ◽  
Digestion Method ◽  
Anabas Testudineus ◽  
Mean Intensity ◽  
Artificial Digestion ◽  
Channa Striata

A survey was performed to investigate the infection status of zoonotic helminth larvae in fish from a local market of North Dagon District in Yangon City, Myanmar. A total of 486 fish in 13 species were collected 8 times from December 2015 to December 2019. All fish were transported under ice to a laboratory in Korea and examined for helminth larvae using artificial digestion method. Larval gnathostomes and metacercariae of more than 8 zoonotic trematode species, i.e., <i>Opisthorchis viverrini, Haplorchis taichui, H. pumilio, H. yokogawai, Centrocestus</i> spp., <i>Stellantchasmus falcatus, Pygidiopsis cambodiensis</i>, and <i>Procerovum</i> sp., were detected. Larval gnathostomes were found in 58 (16.0%) out of 362 fish of 6 species, with mean intensity of 2.8 per fish infected. Metacercariae of <i>O. viverrini</i> were detected in 10 (2.9%) out of 349 fish of 5 species, with mean intensity of 16.9 per fish infected. Metacercarial prevalences of 4 intestinal flukes, <i>H. taichui, H. pumilio, H. yokogawai,</i> and <i>Centrocestus</i> spp., were 16.8%, 26.0%, 12.5%, and 15.0% in the positive fish species, respectively, and mean metacercarial intensity was 63.3, 26.8, 86.2, and 8.7 per fish infected. Metacercariae of <i>S. falcatus</i> and <i>P. cambodiensis</i> were detected only from the mullet, <i>Chelon macrolepis</i>. Metacercariae of <i>Procerovum</i> sp. were found in <i>Channa striata</i> and <i>Anabas testudineus</i>. Collectively, it was confirmed that the fish were infected with gnathostome larvae and metacercariae of <i>O. viverrini</i> and intestinal flukes in Yangon City, Myanmar.

Evaluation of heavy metals content in the canned/packed fruit juices from local and imported origin in Lahore, Pakistan

2021 ◽  
Author(s):  
Nazeefa Fatima ◽  
Munazza Khan ◽  
Muhammad Shuaib Kabeer
Keyword(s):  
Heavy Metals ◽  
Atomic Absorption ◽  
Fruit Juices ◽  
Human Consumption ◽  
Acid Digestion ◽  
Digestion Method ◽  
Mean Values ◽  
Nutritive Values ◽  
The Mean ◽  
Mean Concentration

This study was conducted to determine the mean concentration of heavy metals such as lead (Pb), copper (Cu), zinc (Zn), chromium (Cr), manganese (Mn), nickel (Ni), selenium (Se), magnesium (Mg), and iron (Fe) in canned/packed fruits juices, collected from various stores in Lahore in a period of three months. These juices were categorized into four groups; local packed and canned and also imported packed and canned products. Every group consisted of ten samples. By using the di-acid digestion method, the collected samples were digested and analyzed under Atomic Absorption Spectrophotometer (AAS). The results indicated that the mean values of 7 out of 9 tested heavy metals including Pb, Mg, Ni, Fe, Cr, Se and Mn were above permissible limits (set by WHO) in all four understudy groups. Therefore, it was concluded that commercially available fruit juices are not all safe according to their heavy metals content for the human consumption despite their nutritive values.

scholarly journals THE RELEVANCE OF THE DEFINITION OF BLASTOCYSTIS SPP. IN ENVIRONMENTAL OBJECTS AS POTENTIAL RISK FACTORS FOR THE OCCURRENCE OF PROTOZOAL HUMAN INFECTION

2018 ◽  
Vol 97 (11) ◽  
pp. 1043-1045
Author(s):  
Olga V. Gritsyuk ◽  
K. Yu. Kuznetsova ◽  
A. V. Zagainova
Keyword(s):  
Risk Factors ◽  
Potential Risk ◽  
Practical Importance ◽  
Human Infection ◽  
Survival Duration ◽  
Environmental Objects ◽  
Blastocystis Spp ◽  
Potential Risk Factors ◽  
Infection Risk Factors ◽  
Protozoal Infection

The most abundant protozoan Blastocystis spp. from human and animal intestines is one of the poor-studied pathogens causing the occurrence of a protozoal infection of the human gastrointestinal tract. In Russia, no Blastocyst invasion is recorded and neither included in the forms of state statistical reporting. The manifestations of the epidemic process of blastocyst can be judged based on a small number of studies. Analysis of clinical observations and literature data indicates a variety of forms, localization, and nature of the infection manifestation. This article is devoted to substantiating the need to detect blastocysts in environmental objects (water, soil, surfaces) as potential risk factors for the occurrence of a protozoal infection. At the same time, the studies both of the survival duration in the external environment and influence of various factors the Blastocystis survival have both the theoretical and practical importance. This information can be used in problems of epidemiology and epizootiology, for developing new methods for Blastocystis elimination, and identifying ways of spreading these parasites. Infection risk factors are sanitary and hygienic problems of environmental objects (geographical, environmental and social factors), profession features, contact with animals, use of contaminated water and food, immigration and travel to tropical countries, as well as people with weakened immune status.

scholarly journals Molecular Epidemiology of Anisakis spp. in Wedge Sole, Dicologlossa cuneata (Moreau, 1881), from Fishmarkets in Granada (Southern Spain), Caught in Two Adjacent NE and CE Atlantic Areas

Pathogens ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1302
Author(s):  
Sara Buzo-Domínguez ◽  
Manuel Morales-Yuste ◽  
Ana María Domingo-Hernández ◽  
Rocío Benítez ◽  
Francisco Javier Adroher
Keyword(s):  
Infected Fish ◽  
Human Infection ◽  
Southern Spain ◽  
Type I ◽  
Third Stage ◽  
Traditionally Prepared ◽  
The Mean ◽  
Low Prevalence ◽  
The City ◽  
Anisakis Spp

The presence of third stage larvae (L3) of Anisakis spp. in wedge sole, Dicologlossa cuneata (Moreau, 1881), purchased in fishmarkets in the city of Granada (Andalusia, southern Spain) was assessed. The wedge sole were caught in two FAO zones: area 27.IXa NE Atlantic (SW Spain coast) and area 34.1.11 CE Atlantic (NW Morocco coast). Only Anisakis larvae, type I, were detected in the largest fish (>20 cm) from the CE Atlantic. These were molecularly identified as A. simplex s.s. The prevalence (P) of Anisakis in this area was 12.5% and the mean intensity (MI) was 1. The presence of Hysterothylacium spp. larvae was also detected in the fish from both areas, with the prevalence being approximately double in the CE Atlantic area (12.5 vs. 5.7). A comparison between the Anisakis-infected and non-infected fish from this area showed that the former were significantly longer than the latter (p < 0.01). These results show that Anisakis parasitization of wedge sole sold in the markets of the city of Granada is of low prevalence and intensity (P = 4.5, MI = 1), especially in those from area 27.IXa (P = 0), indicating that the risk of human infection is low, particularly as this fish is traditionally prepared by deep-frying in oil in Andalusia (southern Spain).

scholarly journals 58 Histomorphological Study of Epididymis, ductus deferens and phallus in Adult Male Guinea Fowl (Numida meleagris)

2019 ◽  
Vol 42 (2) ◽  
pp. 58-65
Author(s):  
Shakir M. Mirhish
Keyword(s):  
Copulatory Organ ◽  
Guinea Fowl ◽  
Rete Testis ◽  
Columnar Epithelium ◽  
Local Market ◽  
Ductus Deferens ◽  
Numida Meleagris ◽  
The Mean ◽  
Characteristic Features ◽  
Epididymal Duct

This study was conducted to investigate the anatomical, histological characteristic features of epididymis, ductus deferens and phallus of male guinea fowl (Numidia meleagris ). Fifteen healthy male guinea fowl aged between 18-24 months, weighing 1280 - 1405 gram were used in current study which brought from local market in Baghdad governorate. The epididymis of guinea fowl consisted from rete testis, efferent ductless, connecting duct and epididymal duct. The study revealed that the mean diameter of epididymal ducts was 285.17± 2.43µm lined with pseudostratified columnar epithelium. The epididymal duct continuous with ductus deferens which become wider caudally towards the cloaca, the length of right and left ductus deferens were 9.16±1.20 cm and 9.80±1.24cm respectively. The copulatory organ (phallus) of guinea fowl was not well developed and non-intermittent type consist from right and left lateral lymphoid bodies with median eminences, the transverse width 5.01±0.24mm, the cranio-caudal width 3.51±0.07mm and dorso- ventral width 4.32±0.25mm. The lining epithelium of coprodeum was pseudo stratified columnar epithelium. This study concluded that the epididymis of male guinea fowl located on dorsomedial surface of the testes and without definite parts as in domestic animals, the ductus deferens of guinea fowl was tortuous, the phallus of guinea fowl is non - intermittent type.

Sensitivity of Artificial Digestion and Enzyme Immunoassay Methods of Inspection for Trichinae in Pigs

1998 ◽  
Vol 61 (3) ◽  
pp. 339-343 ◽  
Author(s):  
H. R. GAMBLE
Keyword(s):  
Sample Size ◽  
Enzyme Immunoassay ◽  
Trichinella Spiralis ◽  
Serological Testing ◽  
Digestion Method ◽  
Artificial Digestion ◽  
Eu Directives ◽  
Digestion Methods ◽  
Pooled Sample ◽  
The Eu

Forty-seven pigs were infected with varying doses of Trichinella spiralis and tested for evidence of infection by serology, using an enzyme immunoassay (EIA), and by artificial digestion methods. Using a 1-g sample, as prescribed in accordance with European Union (EU) directives, the sensitivity of the pooled-sample artificial digestion method was between three and five larvae per gram (LPG) of tissue. Using a 5-g sample size, in accordance with methods described in the U.S. Code of Federal Regulations, and as required for the inspection of horses exported to the EU, the sensitivity of the test was increased to approximately 1 LPG. Serological testing by EIA detected pigs with as few as 0.02 LPG, but detection times varied from 4 to 8 weeks after infection. Mean postinoculation times for detection by serology were 32 to 42 days. On the basis of these results, it is clear that digestion testing using a 5-g sample size is the only method of those tested here that is completely reliable for detection of trichinae infection at a level that will protect public health. Both digestion testing using a 1-g sample and EIA have drawbacks. However, EIA remains a highly effective tool for epidemiological purposes and for monitoring trichinae infection on the farm.

Hiding time of the hermit crab Calcinus californiensis: observations from different shell types

2015 ◽  
Vol 93 (7) ◽  
pp. 559-565 ◽  
Author(s):  
Carlos Enrique Chávez-Solís ◽  
Guillermina Alcaraz
Keyword(s):  
Water Column ◽  
Potential Risk ◽  
Hermit Crab ◽  
Hermit Crabs ◽  
Resource Value ◽  
Risk Of Predation ◽  
Potential Risks ◽  
Time Required ◽  
The Cost ◽  
Return To Activity

Hermit crabs respond to predators by hiding in their shells. However, retraction may cause the crab to lose hold of the rock and fall through the water column, and the crab may land with the shell aperture in a different orientation. When the shell is aperture down, hermit crabs return to activity by moving their bodies forward and placing their legs on the ground. In contrast, when the shell is aperture up, crabs need to perform a righting maneuver by extending part of their bodies out of the shell, which makes them more vulnerable to predation. The main goal of this study was to examine the hiding time of the hermit crab Calcinus californiensis Bouvier, 1898 in different shell species under these two different situations to better understand the conflict between the cost of staying in hiding and the risk of predation. The study was conducted in rocky tide pools using seven different shell types that reflect different predator protection and resource value to the crab (based on previous studies). We measured the time required to perform the righting maneuver as a measure of the potential risk incurred when returning to activity. The hiding time with the shell aperture down was not affected by shell species. The requirement to carry out a righting maneuver lengthened the hiding time, which varied in the different shell species according to the time required to recover the natural position. The potential risks associated with the righting time, the protection offered by the shells, and the resource value played an important role in the hiding time of the crabs using different shells. This study shows that hermit crabs are aware of the attributes of the shell and use this information to make decisions about how long to stay hidden after being threatened.

scholarly journals Over prescription of Proton Pump Inhibitors on discharge of Medical Inpatients

2017 ◽  
Vol 18 (1) ◽  
pp. 27-29 ◽  
Author(s):  
Sarmistha Biswas ◽  
ABM Abu Sufian ◽  
Prodip Kumar Sarkar ◽  
Mostofa Kamal Chowdhury ◽  
Joybaer Anam Chowdhury ◽  
...  
Keyword(s):  
Proton Pump Inhibitors ◽  
Proton Pump ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Care Hospital ◽  
Safety And Health ◽  
Medical Inpatients ◽  
Study Population ◽  
Potential Risks ◽  
The Mean

Background: Proton pump inhibitors (PPIs) are the drugs used to treat and prevent acid peptic conditions. Their efficacy and safety profile has led to injudicious prescription of these drugs exposing patients to various potential risks and increased healthcare expenditure.Methodology: A retrospective observational study was done from 10th January to 24th January, 2015 in Medicine Indoor in a tertiary care hospital to determine the trend of prescribing PPIs on discharge of Medical inpatients.Result: We found 117 patients were discharged in the study period; 83 were male and 34 were female. Among them 102 (87.17%) were prescribed PPIs on their discharge. Mean age was 44.79 years. Among the study population only 29 patients (28.5%) truly needed PPIs during their discharge. The mean duration of prescribed PPIs was 28 days.Conclusion: Amore rational prescription of PPI during discharge will have better impact on patient safety and health care expenditure.J MEDICINE January 2017; 18 (1) : 27-29

scholarly journals Spatial Variations and Potential Risks of Heavy Metals in Seawater, Sediments, and Living Organisms in Jiuzhen Bay, China

2020 ◽  
Vol 2020 ◽  
pp. 1-13 ◽  
Author(s):  
Xia Sun ◽  
Bao-Shi Li ◽  
Xuan-Li Liu ◽  
Cheng-Xuan Li
Keyword(s):  
Heavy Metals ◽  
Risk Assessment ◽  
Heavy Metal ◽  
Ecological Risk ◽  
Potential Risk ◽  
Metal Pollution ◽  
Heavy Metal Pollution ◽  
Marine Ecology ◽  
Potential Risks ◽  
Living Organisms

Coastal waters are polluted by heavy metals to varying degrees, posing potential risks to marine ecology and human health. In May 2006, the pollution levels, sources, and ecological risks of heavy metals (Cu, Pb, Zn, Cd, Hg, and As) in seawater, surface sediments, and living organisms were studied in Jiuzhen Bay in Fujian, China. This study identified Hg (0.26–0.72 µg/L) and As (20.3–31.5 µg/L) pollution in the seawater of Jiuzhen Bay. In sediments, heavy Pb pollution (946 µg/g dw) was only detected at one station at a level posing very serious potential risk, while Hg pollution (0.052–0.087 µg/g dw) was observed at three stations at a level posing serious potential risk. No heavy metal pollution was detected in sediments at other stations. The concentrations of five heavy metals (Cu, Zn, As, Cd, and Pb) exceeded the corresponding National Quality Standards for oysters, indicating heavy pollution, based on an ecological risk assessment. In clams, two heavy metals (Pb and As) exceeded the standards, indicating light pollution, based on an ecological risk assessment. No heavy metal pollution was found in fish or shrimps. The heavy metals in the seawater and sediments of Jiuzhen Bay are mainly derived from the river discharges of Luxi and Wujiang Rivers although sewage discharge along the coast of Jiuzhen Bay is another source of heavy metal pollution at some stations. Given the pollution of Pb, Hg, and As in seawater and sediments at some stations within the bay, the potential risks of Pb, Hg, and As in living organisms to both the marine ecology and human health deserve increased attention.

The effect of autologous blood salvage techniques upon bank blood usage and the cost of routine coronary revascularization

Perfusion ◽  
1986 ◽  
Vol 1 (4) ◽  
pp. 239-244 ◽  
Author(s):  
Loren F Hiratzka ◽  
James V Richardson ◽  
Berkeley Brandt ◽  
Donald B Doty ◽  
Nicholas P Rossi ◽  
...  
Keyword(s):  
Coronary Revascularization ◽  
Autologous Blood ◽  
Blood Bank ◽  
Cell Saver ◽  
Homologous Blood ◽  
Homologous Blood Transfusion ◽  
Blood Usage ◽  
Potential Risks ◽  
The Mean ◽  
The Cost

Preservation of autologous blood during cardiac surgery may reduce the need for homologous blood transfusions. We reviewed our experience for patients undergoing primary coronary revascularization to determine the effect of the use of the Haemonetics Cell-Saver upon blood bank resources and upon the cost of operation. The quantity of homologous blood required by two groups of patients was compared. One group of 46 patients had operation prior to use of the Cell- Saver ; the other group of 31 patients was entered into Cell-Saver protocols. The mean number of homologous blood units transfused per patient fell strikingly (p < 0.0001) from 4.2 before to 0.5 after introduction of the Cell- Saver. Of the 31 patients in the Cell-Saver protocol, 71 % required no homologous blood while they received 2.5 units of autologous blood processed by the Cell-Saver. Related to this, the mean number of units prepared by typing and compatibility testing in anticipation of surgery fell from 10 units to five. The projected cost to the patient fell 23%. There were no adverse effects from the use of the Cell-Saver. We conclude that the use of the Cell-Saver is justified not only to reduce the potential risks of homologous blood transfusion, but also to reduce the strain upon blood bank resources and the patient cost of primary coronary revascularization.

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