scholarly journals Concurrent Determination of Vitamin A and β-Carotene in Oil and Fat by High Speed Liquid Chromatography

1977 ◽  
Vol 18 (6) ◽  
pp. 487-492_1 ◽  
Author(s):  
Takenori MARUYAMA ◽  
Toshiaki USHIGUSA ◽  
Hiromu KANEMATSU ◽  
Isao NIIYA ◽  
Masao IMAMURA
Keyword(s):  
Liquid Chromatography ◽  
Vitamin A ◽  
High Speed ◽  
Β Carotene

scholarly journals Simultaneous Determination of all-trans-Retinoic Acid, β-Carotene, and Vitamin A in Galenic Preparations by Liquid Chromatography

2001 ◽  
Vol 84 (2) ◽  
pp. 354-360
Author(s):  
Vincenzo Pucci ◽  
Francesca Bugamelli ◽  
Roberto Mandrioli ◽  
Maria A Raggi
Keyword(s):  
Liquid Chromatography ◽  
Retinoic Acid ◽  
Vitamin A ◽  
Degradation Products ◽  
Extraction Procedure ◽  
Uv Detector ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid ◽  
Β Carotene

Abstract The concentrations of vitamin A, β-carotene, and all-trans-retinoic acid in oral preparations were determined in a single analysis by a method based on isocratic, reversed-phase liquid chromatography (LC). The LC system consisted of a C18 column, a mobile phase of acetonitrile, dichloromethane, methanol, and water and a UV detector set at 330 nm. The linearity ranges were 25–250 ng/mL for trans-retinoic acid and vitamin A, and 100–1000 ng/mL for β-carotene. This LC method for the determination of retinoids is simple, precise, and accurate. No extraction procedure is required before the chromatographic analysis; only a suitable dilution is necessary. The method proved to be reliable, fast, and economical. Furthermore, this method is indicative of stability, because it allows for the determination of degradation products such as 13-cis-retinoic acid.

scholarly journals Simultaneous Determination of Vitamin A and β-Carotene in Dietary Supplements by Liquid Chromatography

2002 ◽  
Vol 85 (5) ◽  
pp. 1127-1135 ◽  
Author(s):  
P Ramnathan Sundaresan
Keyword(s):  
Liquid Chromatography ◽  
Solvent Extraction ◽  
Vitamin A ◽  
Dietary Supplements ◽  
Simultaneous Determination ◽  
Methylene Chloride ◽  
Reversed Phase ◽  
Phase Method ◽  
Β Carotene

Abstract Several liquid chromatography (LC) methods for analysis of vitamin A in oods and feeds have been previously reported but only a few have been applied in non-food matrixes. A validated LC method is needed for determination of vitamin A and β-carotene in the various matrixes presented by dietary supplements. The performance of a reversedphase method with methanol–isopropanol gradient elution was evaluated with standard retinyl derivatives and β-carotene. The reversed-phase method is capable of separating retinol from other derivatives such as retinyl acetate, retinyl palmitate, and β-carotene. Two types of extraction were used to extract the analytes from the dietary supplements: a hexane–methylene chloride extraction for soft-gel capsules containing β-carotene, and a direct solvent extraction for dietary supplements in tablet form. The direct solvent extraction consisted of treatment with ethanol and methylene chloride following addition of hot water (55°C). Results with the reversed-phase method for vitamin A and β-carotene in the products examined ( n = 8) indicated excellent method performance. The main form of vitamin A or β-carotene in dietary supplements was the all trans isomer. The reversed- phase method avoids saponification and is rapid, accurate, precise, and suitable for simultaneous determination of retinyl derivatives and β-carotene in dietary supplements.

A Specific Method for the Determination of Provitamin A Carotenoids in Orange Juice

1975 ◽  
Vol 58 (3) ◽  
pp. 595-598 ◽  
Author(s):  
Samuel K Reeder ◽  
Gary L Park
Keyword(s):  
Liquid Chromatography ◽  
Orange Juice ◽  
High Speed ◽  
Provitamin A ◽  
Specific Method ◽  
Limits Of Detection ◽  
Provitamin A Carotenoids ◽  
Β Carotene

Abstract A method has been developed for rapidly determining the amounts of ∝-carotene, β-carotene, and cryptoxanthin in orange juice. The procedure includes extraction, saponification, and high-speed liquid chromatography. Limits of detection for the 3 carotenoids are 0.04, 0.02, and 0.04 μg/ml, respectively.

scholarly journals Determination of Vitamin A ha Pharmaceutical Preparations by High-Speed Liquid Chromatography

1976 ◽  
Vol 96 (5) ◽  
pp. 669-672 ◽  
Author(s):  
TOMOZOH TAMEGAI ◽  
MASAHIKO OHMAE ◽  
KIYOSHI KAWABE
Keyword(s):  
Liquid Chromatography ◽  
Vitamin A ◽  
High Speed ◽  
Pharmaceutical Preparations

Determination of serum retinol (vitamin A) by high-speed liquid chromatography.

1976 ◽  
Vol 22 (10) ◽  
pp. 1593-1595 ◽  
Author(s):  
M G De Ruyter ◽  
A P De Leenheer
Keyword(s):  
Liquid Chromatography ◽  
Detection Limit ◽  
Vitamin A ◽  
High Speed ◽  
Serum Retinol ◽  
Lower Detection Limit ◽  
Serum Pool ◽  
Lower Detection ◽  
Liquid Chromatographic

Abstract We report a fast and simple high-speed liquid-chromatographic assay for serum retinol. Only 100 mul of serum is required. The lower detection limit is 50 mug/liter; linearity was demonstrated up to 1.50 mg/liter. On analyzing a serum pool eight times, a CV of 2.5% was obtained. Values by this method are compared with results obtained by a flurometric method [Clin. Chem. 16, 766(1970)].

Vitamin A Value of Plant Food Provitamin A - Evaluated by the Stable Isotope Technologies

2014 ◽  
Vol 84 (Supplement 1) ◽  
pp. 25-29 ◽  
Author(s):  
Guangwen Tang
Keyword(s):  
Stable Isotope ◽  
Vitamin A ◽  
Provitamin A ◽  
Animal Origin ◽  
Plant Food ◽  
Plant Foods ◽  
A Value ◽  
Provitamin A Carotenoids ◽  
Β Carotene

Humans need vitamin A and obtain essential vitamin A by conversion of plant foods rich in provitamin A and/or absorption of preformed vitamin A from foods of animal origin. The determination of the vitamin A value of plant foods rich in provitamin A is important but has challenges. The aim of this paper is to review the progress over last 80 years following the discovery on the conversion of β-carotene to vitamin A and the various techniques including stable isotope technologies that have been developed to determine vitamin A values of plant provitamin A (mainly β-carotene). These include applications from using radioactive β-carotene and vitamin A, depletion-repletion with vitamin A and β-carotene, and measuring postprandial chylomicron fractions after feeding a β-carotene rich diet, to using stable isotopes as tracers to follow the absorption and conversion of plant food provitamin A carotenoids (mainly β-carotene) in humans. These approaches have greatly promoted our understanding of the absorption and conversion of β-carotene to vitamin A. Stable isotope labeled plant foods are useful for determining the overall bioavailability of provitamin A carotenoids from specific foods. Locally obtained plant foods can provide vitamin A and prevent deficiency of vitamin A, a remaining worldwide concern.

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