A Specific Method for the Determination of Provitamin A Carotenoids in Orange Juice

Abstract A method has been developed for rapidly determining the amounts of ∝-carotene, β-carotene, and cryptoxanthin in orange juice. The procedure includes extraction, saponification, and high-speed liquid chromatography. Limits of detection for the 3 carotenoids are 0.04, 0.02, and 0.04 μg/ml, respectively.

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Vitamin A Value of Plant Food Provitamin A - Evaluated by the Stable Isotope Technologies

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000183 ◽

2014 ◽

Vol 84 (Supplement 1) ◽

pp. 25-29 ◽

Cited By ~ 7

Author(s):

Guangwen Tang

Keyword(s):

Stable Isotope ◽

Vitamin A ◽

Provitamin A ◽

Animal Origin ◽

Plant Food ◽

Plant Foods ◽

A Value ◽

Provitamin A Carotenoids ◽

Β Carotene

Humans need vitamin A and obtain essential vitamin A by conversion of plant foods rich in provitamin A and/or absorption of preformed vitamin A from foods of animal origin. The determination of the vitamin A value of plant foods rich in provitamin A is important but has challenges. The aim of this paper is to review the progress over last 80 years following the discovery on the conversion of β-carotene to vitamin A and the various techniques including stable isotope technologies that have been developed to determine vitamin A values of plant provitamin A (mainly β-carotene). These include applications from using radioactive β-carotene and vitamin A, depletion-repletion with vitamin A and β-carotene, and measuring postprandial chylomicron fractions after feeding a β-carotene rich diet, to using stable isotopes as tracers to follow the absorption and conversion of plant food provitamin A carotenoids (mainly β-carotene) in humans. These approaches have greatly promoted our understanding of the absorption and conversion of β-carotene to vitamin A. Stable isotope labeled plant foods are useful for determining the overall bioavailability of provitamin A carotenoids from specific foods. Locally obtained plant foods can provide vitamin A and prevent deficiency of vitamin A, a remaining worldwide concern.

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High-Speed Liquid Chromatographic Determination of Biphenyl Residues in Citrus Products

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/58.5.1013 ◽

1975 ◽

Vol 58 (5) ◽

pp. 1013-1014

Author(s):

Samuel K Reeder

Keyword(s):

Liquid Chromatography ◽

Rapid Method ◽

Orange Juice ◽

High Speed ◽

Extraction Procedure ◽

Chromatographic Determination ◽

Liquid Chromatographic Determination ◽

Liquid Chromatographic

Abstract A simple, direct, and rapid method is given for the analysis of citrus oils for the fungistat biphenyl by high-speed liquid chromatography. The method is extended to orange juice and various “dry flavors” by an extraction procedure. Analytical limits are <1 ppm without need for any cleanup or concentration steps.

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Determination of Carotenoids in Yellow Maize, the Effects of Saponification and Food Preparations

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831.78.3.112 ◽

2008 ◽

Vol 78 (3) ◽

pp. 112-120 ◽

Cited By ~ 40

Author(s):

Muzhingi ◽

Yeum ◽

Russell ◽

Johnson ◽

Qin ◽

...

Keyword(s):

High Performance ◽

Provitamin A ◽

Carotenoid Content ◽

Carotenoid Concentration ◽

Eye Health ◽

Provitamin A Carotenoids ◽

C30 Column ◽

Β Carotene ◽

Food Consumed

Maize is an important staple food consumed by millions of people in many countries. Yellow maize naturally contains carotenoids which not only provide provitamin A carotenoids but also xanthophylls, which are known to be important for eye health. This study was aimed at 1) evaluating the effect of saponification during extraction of yellow maize carotenoids, 2) determining the major carotenoids in 36 genotypes of yellow maize by high-performance liquid chromatography with a C30 column, and 3) determining the effect of cooking on the carotenoid content of yellow maize. The major carotenoids in yellow maize were identified as all-trans lutein, cis-isomers of lutein, all-trans zeaxanthin, α- and β-cryptoxanthin, all-trans β-carotene, 9-cis β-carotene, and 13-cis β-carotene. Our results indicated that carotenoid extraction without saponification showed a significantly higher yield than that obtained using saponification. Results of the current study indicate that yellow maize is a good source of provitamin A carotenoids and xanthophylls. Cooking by boiling yellow maize at 100° C for 30 minutes increased the carotenoid concentration, while baking at 450° F for 25 minutes decreased the carotenoid concentrations by almost 70% as compared to the uncooked yellow maize flour.

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High Performance Liquid Chromatographic Determination of Provitamin A in Orange Juice

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/60.1.132 ◽

1977 ◽

Vol 60 (1) ◽

pp. 132-136 ◽

Cited By ~ 1

Author(s):

Ivan Stewart

Keyword(s):

Orange Juice ◽

High Performance ◽

Chromatographic Determination ◽

High Performance Liquid Chromatographic ◽

Provitamin A ◽

Provitamin A Carotenoids ◽

Single Column ◽

Liquid Chromatographic Determination ◽

Liquid Chromatographic

Abstract A quantitative method has been developed for the determination of α- and β-carotenes and β-cryptoxanthin, the provitamin A carotenoids in orange juice. The carotenoids were separated by high performance liquid chromatography on a single column in approximately 30 min. The procedure may also be used to measure zeta-carotene and α-cryptoxanthin.

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Concurrent Determination of Vitamin A and β-Carotene in Oil and Fat by High Speed Liquid Chromatography

Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) ◽

10.3358/shokueishi.18.487 ◽

1977 ◽

Vol 18 (6) ◽

pp. 487-492_1 ◽

Cited By ~ 6

Author(s):

Takenori MARUYAMA ◽

Toshiaki USHIGUSA ◽

Hiromu KANEMATSU ◽

Isao NIIYA ◽

Masao IMAMURA

Keyword(s):

Liquid Chromatography ◽

Vitamin A ◽

High Speed ◽

Β Carotene

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Micro-Determination of p-Hydroxybenzoic Esters in Pharmaceuticals and Cosmetics

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/60.1.56 ◽

1977 ◽

Vol 60 (1) ◽

pp. 56-59 ◽

Cited By ~ 1

Author(s):

Emil Weisenberg ◽

Baruch Gershon ◽

Judith Schoenberg

Keyword(s):

Liquid Chromatography ◽

Phosphate Ester ◽

Specific Method ◽

Gas Liquid Chromatography ◽

Direct Extraction ◽

Flame Photometric Detector ◽

Phenol Derivatives ◽

Margin Of Error ◽

Diethyl Phosphate

Abstract A rapid and specific method is described for the determination of microamounts of methyl, propyl, and butyl p-hydroxybenzoic esters (parabens) in pharmaceuticals and cosmetics. The method involves the direct extraction of parabens into benzene or chloroform followed by derivatization with phosphorochloridate. The diethyl phosphate ester derivatives are cleaned up on a Florisil minicolumn and finally measured by gas-liquid chromatography on 5% OV-210 on Gas-Chrom Q. A flame photometric detector or a KCl thermionic detector may be used. The concentration response was linear up to 40 ng parabens. The reproducibility and margin of error were tested with fortified samples. This method may be applied to the estimation of other phenol derivatives.

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