Starch and triacylglycerol accumulation in the cells of the stain Chlorella sp. IPPAS C-1210

2021 ◽  
pp. 1-7
Author(s):  
Lidia A. Bobrovnikova ◽  
Maria S. Pakholkova ◽  
Roman A. Sidorov ◽  
Maria A. Sinetova
Keyword(s):  
Carbon Dioxide ◽  
Lipid Content ◽  
Food Industry ◽  
Nitrogen Starvation ◽  
Total Lipid Content ◽  
Biomass Productivity ◽  
Chlorella Sp ◽  
Phosphorus Source ◽  
Triacylglycerol Accumulation ◽  
In Cells

Strain Сhlorella sp. IPPAS C-1210 is an effective lipid and triacilglycerols (TAG) producer. The strain could be used eventually in such industries as bioenergetics, food industry and agriculture. The objective of this work was investigation of conditions in which the strain Сhlorella sp. IPPAS C-1210 accumulates the most starch and TAG in cells with a view to optimise its growth and productivity. The following cultivation parameters were investigated in order to figure out their influence on accumulation of starch and TAG: nitrogen- and phosphorous-starvation and cultivation on media with different nitrogen (nitrate, urea) and carbon (carbon dioxide, bicarbonate) sources. Pigments, starch, protein and lipid content in cells were measured. The exclusion of nitrogen or phosphorus source from medium decreased the biomass productivity significantly, caused chlorosis and reduction of protein content. Total lipid content increased slightly after phosphorous starvation and stayed almost constant under nitrogen starvation, however a greater TAG increase was observed during nitrogen starvation. Both nitrogen and phosphorous starvations caused the increase of the amount of reserve carbohydrates: during phosphorous starvation increase was insignificant, whereas the latter almost doubled the amount of reserve carbohydrates. The highest biomass and lipid productivity was observed in cells grown in bicarbonate supplement medium and the highest starch productivity was observed in cells grown in standard BBM-3N medium.

scholarly journals The Use of Urea and Kelp Waste Extract is A Promising Strategy for Maximizing the Biomass Productivity and Lipid Content in Chlorella sorokiniana

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 463 ◽  
Author(s):  
Ali Nawaz Kumbhar ◽  
Meilin He ◽  
Abdul Razzaque Rajper ◽  
Khalil Ahmed Memon ◽  
Muhammad Rizwan ◽  
...  
Keyword(s):  
Lipid Content ◽  
Fossil Fuels ◽  
Fossil Fuel ◽  
Lipid Production ◽  
Total Lipid Content ◽  
Biomass Productivity ◽  
Chlorella Sorokiniana ◽  
Biofuel Production ◽  
Promising Strategy ◽  
Waste Extract

The decline in fossil fuel reserves has forced researchers to seek out alternatives to fossil fuels. Microalgae are considered to be a promising feedstock for sustainable biofuel production. Previous studies have shown that urea is an important nitrogen source for cell growth and the lipid production of microalgae. The present study investigated the effect of different concentrations of urea combined with kelp waste extract on the biomass and lipid content of Chlorella sorokiniana. The results revealed that the highest cell density, 20.36 × 107 cells−1, and maximal dry biomass, 1.70 g/L, were achieved in the presence of 0.5 g/L of urea combined with 8% kelp waste extract. Similarly, the maximum chlorophyll a, b and beta carotenoid were 10.36 mg/L, 7.05, and 3.01 mg/L, respectively. The highest quantity of carbohydrate content, 290.51 µg/mL, was achieved in the presence of 0.2 g/L of urea and 8% kelp waste extract. The highest fluorescence intensity, 40.05 × 107 cells−1, and maximum total lipid content (30%) were achieved in the presence of 0.1 g/L of urea and 8% kelp waste extract. The current study suggests that the combination of urea and kelp waste extract is the best strategy to enhance the biomass and lipid content in Chlorella sorokiniana.

scholarly journals Bio-Mitigation of Carbon Dioxide Using Desmodesmus sp. in the Custom-Designed Pilot-Scale Loop Photobioreactor

2021 ◽  
Vol 13 (17) ◽  
pp. 9882
Author(s):  
Abhishek Anand ◽  
Kaustubh Tripathi ◽  
Amit Kumar ◽  
Suresh Gupta ◽  
Smita Raghuvanshi ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Global Warming ◽  
Lipid Content ◽  
Algal Biomass ◽  
Scale Up ◽  
Biomass Productivity ◽  
Co2 Concentration ◽  
Management Systems ◽  
Fixation Rate ◽  
Chl A

Today’s society is faced with many upfront challenges such as the energy crisis, water pollution, air pollution, and global warming. The greenhouse gases (GHGs) responsible for global warming include carbon dioxide (CO2), methane (CH4), nitrous oxide (NOx), water vapor (H2O), and fluorinated gases. A fraction of the increased emissions of CO2 in the atmosphere is due to agricultural and municipal solid waste (MSW) management systems. There is a need for a sustainable solution which can degrade the pollutants and provide a technology-based solution. Hence, the present work deals with the custom design of a loop photobioreactor with 34 L of total volume used to handle different inlet CO2 concentrations (0.03%, 5%, and 10% (v/v)). The obtained values of biomass productivity and CO2 fixation rate include 0.185 ± 0.004 g L−1 d−1 and 0.333 ± 0.004 g L−1 d−1, respectively, at 10% (v/v) CO2 concentration and 0.084 ± 0.003 g L−1 d−1 and 0.155 ± 0.003 g L−1 d−1, respectively, at 5% (v/v) CO2 concentration. The biochemical compositions, such as carbohydrate, proteins, and lipid content, were estimated in the algal biomass produced from CO2 mitigation studies. The maximum carbohydrate, proteins, and lipid content were obtained as 20.7 ± 2.4%, 32.2 ± 2.5%, and 42 ± 1.0%, respectively, at 10% (v/v) CO2 concentration. Chlorophyll (Chl) a and b were determined in algal biomass as an algal physiological response. The results obtained in the present study are compared with the previous studies reported in the literature, which indicated the feasibility of the scale-up of the process for the source reduction of CO2 generated from waste management systems without significant change in productivity. The present work emphasizes the cross-disciplinary approach for the development of bio-mitigation of CO2 in the loop photobioreactor.

scholarly journals Identification of the molecular regulation of differences in lipid deposition in dedifferentiated preadipocytes from different chicken tissues

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Zheng Ma ◽  
Na Luo ◽  
Lu Liu ◽  
Huanxian Cui ◽  
Jing Li ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Metabolism ◽  
Lipid Content ◽  
Regulatory Network ◽  
Total Lipid Content ◽  
Fatty Acid Transport ◽  
Lipid Deposition ◽  
Ppar Signaling ◽  
The Difference

Abstract Background A body distribution with high intramuscular fat and low abdominal fat is the ideal goal for broiler breeding. Preadipocytes with different origins have differences in terms of metabolism and gene expression. The transcriptome analysis performed in this study of intramuscular preadipocytes (DIMFPs) and adipose tissue-derived preadipocytes (DAFPs) aimed to explore the characteristics of lipid deposition in different chicken preadipocytes by dedifferentiation in vitro. Results Compared with DAFPs, the total lipid content in DIMFPs was reduced (P < 0.05). Moreover, 72 DEGs related to lipid metabolism were screened, which were involved in adipocyte differentiation, fatty acid transport and fatty acid synthesis, lipid stabilization, and lipolysis. Among the 72 DEGs, 19 DEGs were enriched in the PPAR signaling pathway, indicating its main contribution to the regulation of the difference in lipid deposition between DAFPs and DIMFPs. Among these 19 genes, the representative APOA1, ADIPOQ, FABP3, FABP4, FABP7, HMGCS2, LPL and RXRG genes were downregulated, but the ACSL1, FABP5, PCK2, PDPK1, PPARG, SCD, SCD5, and SLC27A6 genes were upregulated (P < 0.05 or P < 0.01) in the DIMFPs. In addition, the well-known pathways affecting lipid metabolism (MAPK, TGF-beta and calcium) and the pathways related to cell communication were enriched, which may also contribute to the regulation of lipid deposition. Finally, the regulatory network for the difference in lipid deposition between chicken DAFPs and DIMFPs was proposed based on the above information. Conclusions Our data suggested a difference in lipid deposition between DIMFPs and DAFPs of chickens in vitro and proposed a molecular regulatory network for the difference in lipid deposition between chicken DAFPs and DIMFPs. The lipid content was significantly increased in DAFPs by the direct mediation of PPAR signaling pathways. These findings provide new insights into the regulation of tissue-specific fat deposition and the optimization of body fat distribution in broilers.

Aspergillus flavusgrowth and aflatoxin production as influenced by total lipid content during growth and development of cottonseed

2017 ◽  
Vol 31 (1) ◽  
pp. 91-99 ◽  
Author(s):  
Kanniah Rajasekaran ◽  
Greg Ford ◽  
Kandan Sethumadhavan ◽  
Carol Carter-Wientjes ◽  
John Bland ◽  
...  
Keyword(s):  
Total Lipid ◽  
Lipid Content ◽  
Growth And Development ◽  
Total Lipid Content ◽  
Aflatoxin Production

Comparison of biomass production and total lipid content of freshwater green microalgae cultivated under various culture conditions

2013 ◽  
Vol 37 (2) ◽  
pp. 99-106 ◽  
Author(s):  
Geun Ho Gim ◽  
Jung Kon Kim ◽  
Hyeon Seok Kim ◽  
Mathur Nadarajan Kathiravan ◽  
Hetong Yang ◽  
...  
Keyword(s):  
Biomass Production ◽  
Total Lipid ◽  
Lipid Content ◽  
Total Lipid Content ◽  
Culture Conditions ◽  
Green Microalgae
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