Some thoughts of an American scientist on the dynamic high-pressure work of Academician Ya B Zel'dovich

1995 ◽  
Vol 165 (5) ◽  
pp. 595 ◽  
Author(s):  
R.N. Keeler
Keyword(s):  
High Pressure ◽  
Pressure Work ◽  
Dynamic High Pressure ◽  
American Scientist

scholarly journals A High Throughput Approach Based on Dynamic High Pressure for the Encapsulation of Active Compounds in Exosomes for Precision Medicine

2021 ◽  
Vol 22 (18) ◽  
pp. 9896
Author(s):  
Eugenia Romano ◽  
Paolo Antonio Netti ◽  
Enza Torino
Keyword(s):  
Cell Culture ◽  
High Pressure ◽  
Culture Media ◽  
Preliminary Test ◽  
Bilayer Membrane ◽  
Microfluidic System ◽  
Culture Model ◽  
Before And After ◽  
Dynamic High Pressure

In recent decades, endogenous nanocarrier-exosomes have received considerable scientific interest as drug delivery systems. The unique proteo-lipid architecture allows the crossing of various natural barriers and protects exosomes cargo from degradation in the bloodstream. However, the presence of this bilayer membrane as well as their endogenous content make loading of exogenous molecules challenging. In the present work, we will investigate how to promote the manipulation of vesicles curvature by a high-pressure microfluidic system as a ground-breaking method for exosomes encapsulation. Exosomes isolated from Uppsala 87 Malignant Glioma (U87-MG) cell culture media were characterized before and after the treatment with high-pressure homogenization. Once their structural and biological stability were validated, we applied this novel method for the encapsulation in the lipidic exosomal bilayer of the chemotherapeutic Irinotecan HCl Trihydrate-CPT 11. Finally, we performed in vitro preliminary test to validate the nanobiointeraction of exosomes, uptake mechanisms, and cytotoxic effect in cell culture model.

Modification of food macromolecules using dynamic high pressure microfluidization: A review

2020 ◽  
Vol 100 ◽  
pp. 223-234 ◽  
Author(s):  
Xiaojuan Guo ◽  
Mingshun Chen ◽  
Yuting Li ◽  
Taotao Dai ◽  
Xixiang Shuai ◽  
...  
Keyword(s):  
High Pressure ◽  
Dynamic High Pressure Microfluidization ◽  
Dynamic High Pressure
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