The effect of refined functional carbohydrates from enzymatically hydrolyzed yeast on the transmission of environmental Salmonella Senftenberg among broilers and proliferation in broiler housing

The USDA has established processing schedules for beef products based on the destruction of pathogens. Several enzymes have been suggested as potential indicators of heat processing. However, no relationship between the inactivation rates of these enzymes and those of pathogenic microorganisms has been determined. Our objective was to compare the thermal inactivation of Escherichia coli O157:H7 and Salmonella senftenberg to those of endogenous muscle proteins. Inoculated and noninoculated ground beef samples were heated at four temperatures for predetermined intervals of time in thermal-death-time studies. Bacterial counts were determined and enzymes were assayed for residual activity. The D values for E. coli O157:H7 were 46.10, 6.44, 0.43, and 0.12 min at 53, 58, 63, and 68°C, respectively, with a z value of 5.60°C. The D values for S. senftenberg were 53.00, 15.17, 2.08, and 0.22 min at 53, 58, 63, and 68°C, respectively, with a z value of 6.24°C. Apparent D values at 53, 58, 63, and 68°C were 352.93, 26.31, 5.56, and 3.33 min for acid phosphatase; 6968.64, 543.48, 19.61, and 1.40 min for lactate dehydrogenase; and 3870.97, 2678.59, 769.23, and 42.92 min for peroxidase; with z values of 7.41,3.99, and 7.80°C, respectively. Apparent D values at 53, 58, 63, and 66°C were 325.03, 60.07, 3.07, and 1.34 min for phosphoglycerate mutase; 606.72, 89.86, 4.40, and 1.28 min for glyceraldehyde-3-phosphate dehydrogenase; and 153.06, 20.13, 2.25, and 0.74 min for triose phosphate isomerase; with z values of 5.18, 4.71, and 5.56°C, respectively. The temperature dependence of triose phosphate isomerase was similar to those of both E. coli O157 :H7 and S. senftenberg, suggesting that this enzyme could be used as an endogenous time-temperature indicator in beef products.

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Epizootiologic Role of Feeds in the Epidemiology of Salmonella Senftenberg Contamination in Commercial Layer Farms in Eastern Japan

Avian Diseases Digest ◽

10.1637/10231-996412-digest.1 ◽

2012 ◽

Vol 7 (3) ◽

pp. e42-e43

Author(s):

Kazutoshi Shirota ◽

Dennis V. Umali ◽

Terumasa Suzuki ◽

Hiromitsu Katoh

Keyword(s):

Salmonella Senftenberg ◽

Eastern Japan

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Colonization of Broiler Chicks by Salmonella Typhimurium Definitive Phage Type 104

Journal of Food Protection ◽

10.4315/0362-028x-64.11.1698 ◽

2001 ◽

Vol 64 (11) ◽

pp. 1698-1704 ◽

Cited By ~ 11

Author(s):

PAULA J. FEDORKA-CRAY ◽

SCOTT R. LADELY ◽

J. STAN BAILEY ◽

NORMAN J. STERN

Keyword(s):

Salmonella Typhimurium ◽

Broiler Chickens ◽

Phage Type ◽

Control Group ◽

Broiler Chicks ◽

Control Groups ◽

Salmonella Spp ◽

And Control ◽

Salmonella Senftenberg ◽

Over Time

The prevalence of an antibiotic-resistant strain of Salmonella Typhimurium definitive phage type 104 (DT104) has increased dramatically in recent years resulting in increased morbidity and mortality in both animals and humans. Colonization and shedding of Salmonella Typhimurium DT104 was studied in broiler chickens in two trials. In trial 1, 180 day-of-hatch chicks (n = 60 per group, n = 30 per replicate) were challenged with 106 CFU DT104 (wild-type isolate from poultry) or were commingled with a seeder chick challenged with 106 CFU DT104. In trial 2, 360 day-of-hatch chicks (n = 120 per treatment, n = 30 per rep) were divided into three groups. Chicks in the susceptible group were commingled with two seeder chicks that were orally challenged with 107 CFU/bird of a pan-sensitive strain of Salmonella Typhimurium DT104. Chicks in the resistant group were commingled with two seeder chicks that were orally challenged with 107 CFU/bird DT104 used in trial 1. For both trials, a control group was not exposed to DT104, composite fecal samples were evaluated twice weekly for levels of Salmonella shedding and 20 chicks per group were necropsied weekly and their cecal contents were cultured. At hatch all groups were colonized with naturally occurring Salmonella Senftenberg and Salmonella Mbandaka (trial 1) or Salmonella Senftenberg and Salmonella Ohio (trial 2) prior to exposure to DT104. Throughout the study, the level of Salmonella spp. shedding in feces (trial 1 means 3.1, 2.9, and 3.0 log10 CFU per g feces for challenged, seeder, and control groups, respectively) or ceca (trial 2 means 2.9, 2.9, and 2.5 log10 CFU per g ceca for resistant, susceptible, and control groups, respectively)did not differ among groups. In trial 1, colonization of DT104 remained constant at higher levels in the challenged group (mean 87%, P < 0.01), increased over time in the seeder group (10 to 50%, P < 0.02) and was not recovered from the control chicks. Salmonella Mbandaka colonization remained steady within each group with challenge and seeder groups maintaining higher levels of colonization than the control group. Salmonella Senftenberg colonization levels tended to decline (P = .058) over time in the challenged group (20 to 0%) and significantly decreased (P < 0.01) over time for both the seeder (80 to 0%) and control chicks (85 to 10%). In trial 2, the percentage of chicks colonized with susceptible DT104 declined (r = 0.90, P < 0.05) over the course of the trial from 45 to 0%, while recovery of the resistant DT104 persisted at a mean percentage of 27%. DT104 was not recovered from the control chicks. Salmonella Ohio colonization levels tended to decline (r = 0.79, P > 0.05) over time in the control group (75 to 20%) and significantly decreased (P < 0.05) over time in both susceptible and resistant groups (40 to 10%, r = 0.82 and 55 to 5%, r = 0.85, respectively). Salmonella Senftenberg was recovered from the control group at low frequency throughout the trial and was not recovered from the other groups. For either trial, no apparent affect on morbidity or mortality was observed. Introduction of DT104 by commingling may induce colonization resulting in persistent high levels of shedding in flocks simultaneously with other Salmonella species.

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Bali Belly: Salmonella senftenberg Found in an Infected Ovarian Endometrioma

Case Reports in Obstetrics and Gynecology ◽

10.1155/2020/3581091 ◽

2020 ◽

Vol 2020 ◽

pp. 1-3

Author(s):

P. D. M. Pathiraja ◽

Junaid Rafi ◽

Emily Woolnough ◽

Anna Clare

Keyword(s):

Septic Shock ◽

Abdominal Pain ◽

Pelvic Inflammatory Disease ◽

Antibiotic Treatment ◽

Bacterial Translocation ◽

Inflammatory Disease ◽

Peritoneal Fluid ◽

Ovarian Endometrioma ◽

Surgical Evacuation ◽

Salmonella Senftenberg

Salmonella is an extremely rare cause of an infected endometrioma. We present a case of a 30-year-old immunocompetent woman presenting with fevers and abdominal pain, on a background of prior endometriosis. Initial antibiotic treatment for pelvic inflammatory disease failed, and the patient progressed to septic shock requiring surgical evacuation of an infected ovarian endometrioma. Microbiological samples from stool, ovary, and peritoneal fluid revealed infection with Salmonella senftenberg. The likely diagnosis was Salmonella enterocolitis with bacterial translocation to an ovarian endometrioma.

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Identification of Salmonella Serovars Isolated from Live Molluscan Shellfish and Their Significance in the Marine Environment

Journal of Food Protection ◽

10.4315/0362-028x-66.2.226 ◽

2003 ◽

Vol 66 (2) ◽

pp. 226-232 ◽

Cited By ~ 39

Author(s):

JAIME MARTINEZ-URTAZA ◽

MONTSERRAT SACO ◽

GUSTAVO HERNANDEZ-CORDOVA ◽

ANTONIO LOZANO ◽

OSCAR GARCIA-MARTIN ◽

...

Keyword(s):

Marine Environment ◽

Fecal Coliform ◽

Seasonal Pattern ◽

Maximum Level ◽

Fecal Coliforms ◽

Salmonella Spp ◽

Low Levels ◽

Slight Rise ◽

Contamination Events ◽

Salmonella Senftenberg

A study on the presence of Salmonella spp. in live molluscs was performed, which included a description of the different serovars isolated and their relationship to the marine environment. A total of 2,980 samples of shellfish from Galicia (N.W. Spain) were tested for the presence of Salmonella spp. between September 1998 and August 2001. The overall incidence of Salmonella was 1.8% and showed a slight rise during the 3 years of the study. Mussels and oysters presented a higher incidence than clams and cockles, possibly because of their distinct growing habitat. A seasonal pattern was noted for the isolation of Salmonella spp.: 54% of the isolations were detected from September to November. That nearly 67% of the total Salmonella was isolated from shellfish with fecal coliform levels <300/100 g (the maximum level criteria in the European Communities regulations) supported the view that low levels of fecal coliforms do not necessarily indicate the absence of Salmonella. A total of nine serovars were found in the 54 Salmonella isolated. Salmonella Senftenberg was the most frequent (50%), followed by Salmonella Typhimurium (18%) and Salmonella Agona (17%). Salmonella Senftenberg was detected frequently during the year, whereas the remaining serovars were detected only on occasional contamination events.

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Alternation of Low and High Affinities of Secreted and Cell-bound Antibodies during the Anamnestic Response of Rabbits to Salmonella senftenberg Microorganisms

Scandinavian Journal of Immunology ◽

10.1111/j.1365-3083.1979.tb02710.x ◽

1979 ◽

Vol 9 (1) ◽

pp. 87-98 ◽

Cited By ~ 3

Author(s):

R. CHABY ◽

R. GFRARD ◽

E. DUFLOT

Keyword(s):

Anamnestic Response ◽

Salmonella Senftenberg

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