Dietary effects of Bacillus subtilis fmbj on growth performance, small intestinal morphology, and its antioxidant capacity of broilers

AbstractThe present study was conducted to investigate effects of Bacillus subtilis on growth performance, serum parameters, digestive enzymes, intestinal morphology, and colonic microbiota in piglets. A total of 72 piglets were weighed and randomly allotted into three treatments (four replication pens per treatment with six piglets/pen) for a 28-day experiment. The dietary treatments were as follows: basal diet (control group, CTR), basal diet supplementation with antibiotic (antibiotic group, ABT), and basal diet supplementation with 0.1% Bacillus subtilis (probiotic group, PBT). The average daily gain of body weight increased in both the ABT and PBT groups, and dietary antibiotics decreased the feed:gain ratio (F:G), as compared to the CTR group (P < 0.05). Both ABT and PBT piglets had increased serum triglycerides and lipase, amylase, maltase activities and villus height:crypt depth ratio (V/C) in ileum (P < 0.05). The PBT group also showed an increase in serum glucose and villus height in the ileum (P < 0.05). Dietary antibiotics increased Lactobacillus johnsonii, as compared to the CTR group, but decreased bacterial diversity and increased Escherichia coli, as compared to the PBT group (P < 0.05). Piglets dietary with B. subtilis modulated the microbiota by increasing the abundance of Firmicutes (L. johnsonii, L. reuteri) and decreasing the abundance of E. coli, as compared to the control group (P < 0.05). These results indicate that dietary of B. subtilis improves growth performance and intestinal health and can be a promising alternative to antibiotics in piglets diet.

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Comparison effect of Rhodobacter sphaeroides protein replace fishmeal on growth performance, intestinal morphology, hepatic antioxidant capacity and immune gene expression of Litopenaeus vannamei under low salt stress

Aquaculture ◽

10.1016/j.aquaculture.2021.737488 ◽

2021 ◽

pp. 737488

Author(s):

Zhihong Liao ◽

Yangyang Gong ◽

Wei Zhao ◽

Xuanshu He ◽

Dan Wei ◽

...

Keyword(s):

Gene Expression ◽

Salt Stress ◽

Antioxidant Capacity ◽

Growth Performance ◽

Rhodobacter Sphaeroides ◽

Litopenaeus Vannamei ◽

Intestinal Morphology ◽

Immune Gene ◽

Immune Gene Expression ◽

Low Salt

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Effects of graded levels of xylo-oligosaccharides on growth performance, serum parameters, intestinal morphology and intestinal barrier function in weaned piglets

Journal of Animal Science ◽

10.1093/jas/skab183 ◽

2021 ◽

Author(s):

Yuxia Chen ◽

Yining Xie ◽

Ruqing Zhong ◽

Hui Han ◽

Lei Liu ◽

...

Keyword(s):

Growth Performance ◽

Intestinal Barrier ◽

Intestinal Morphology ◽

Intestinal Barrier Function ◽

Weaned Piglets ◽

Villus Height ◽

Expression Levels ◽

Small Intestinal ◽

Dietary Treatments ◽

Mrna Expression Levels

Abstract The objective of this study was to investigate the effects of xylo-oligosaccharides (XOS) supplementation on growth performance, serum parameters, small intestinal morphology, intestinal mucosal integrity, and immune function in weaned piglets. A total of 240 weaned piglets with an average body weight (BW) of 8.82 ± 0.05 kg (28 d of age) were assigned randomly to 4 dietary treatments in a 28-d trial, including a control diet (CON), 3 diets with XOS supplementation at the concentration of 100, 500 and 1000 mg/kg (XOS100, XOS500, and XOS1000). There were 4 replicates per treatment with 15 pigs per pen. From d 1 to 14, there were no differences (P > 0.05) in average daily gain (ADG), average daily feed intake (ADFI), and gain to feed ratio (G:F) during the different treatments. The different doses of XOS showed a quadratic effect on BW on d 28, ADG and G:F d 1-28 of piglets (P < 0.05). From d 15 to 28, ADG of pigs fed the XOS500 diet was higher (P < 0.05) than pigs fed the CON diet. During the overall period (d 1 to 28), pigs fed the XOS500 diet had a higher BW, ADG and G:F than pigs fed the CON diet (P < 0.05). In addition, compared with the CON group, the XOS500 group had significantly higher serum total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD) and catalase (CAT) levels and lower malondialdehyde (MDA) levels on d 14 and 28 (P < 0.05). The serum immunoglobulin G (IgG) concentration in the XOS500 group was also significantly higher compared with the CON group on d 14 and 28 (P <0.05). However, serum immunoglobulin A (IgA) and immunoglobulin M (IgM) were not affected by the dietary treatments. Supplementation of XOS500 to the feed significantly increased the villus height (VH) and villus height to crypt depth ratio (VH:CD) in the jejunum and ileum in comparison with the CON and XOS1000 group. Moreover, the XOS500 group significantly elevated the expression levels of Occludin and zonula occludens protein-1 (ZO-1) in the ileum compared to the CON group. The ileal interleukin (IL)-1β, IL-8 and interferon (IFN)-γ mRNA expression levels in the XOS100 and XOS500 group were markedly lower than in the CON group. In contrast, the ileal IL-10 mRNA expression levels were remarkably higher in the XOS500 than CON group. In conclusion, xylo-oligosaccharides have a beneficial effect on growth performance by improving serum antioxidant defense system, serum IgG, small intestinal structure and intestinal barrier function in weaned piglets.

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Effect of dietary supplementation of whey powder and Bacillus subtilis on growth performance, gut and hepatic function, and muscle antioxidant capacity of Japanese quail

Journal of Animal Physiology and Animal Nutrition ◽

10.1111/jpn.13323 ◽

2020 ◽

Vol 104 (3) ◽

pp. 886-897 ◽

Cited By ~ 1

Author(s):

Vahid Jazi ◽

Majid Farahi ◽

Fariborz Khajali ◽

Shaymma Abousaad ◽

Peter Ferket ◽

...

Keyword(s):

Bacillus Subtilis ◽

Antioxidant Capacity ◽

Growth Performance ◽

Japanese Quail ◽

Dietary Supplementation ◽

Hepatic Function ◽

Whey Powder

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Dietary chitosan-Cu chelate affects growth performance and small intestinal morphology and apoptosis in weaned piglets.

Czech Journal of Animal Science ◽

10.17221/85/2015-cjas ◽

2017 ◽

Vol 62 (No. 1) ◽

pp. 15-21

Author(s):

X. Yue ◽

L. Hu ◽

X. Fu ◽

M. Lv ◽

X. Han

Keyword(s):

Epithelial Cell ◽

Growth Performance ◽

Cell Apoptosis ◽

Intestinal Morphology ◽

Weaned Piglets ◽

Villus Height ◽

Crypt Depth ◽

Epithelial Cell Apoptosis ◽

Diarrhea Incidence ◽

Small Intestinal

The effects of dietary chitosan-copper chelate (CS-Cu) on growth performance, diarrhea, intestinal morphology and epithelial cell apoptosis in weaned piglets was investigated. One hundred and sixty Duroc × Landrace × Yorkshire weanling barrows with an average body weight of 7.75 kg were randomly assigned to one of the following dietary treatments: (1) control, (2) 100 mg Cu/kg diet from CuSO4, (3) 100 mg Cu/kg diet from CuSO4 mixed with chitosan (CuSO4+CS), (4) 100 mg Cu/kg diet from CS-Cu. The feeding trial lasted for 30 days. The results showed that the pigs receiving a diet containing CS-Cu had higher average daily gain and lower diarrhea incidence than the pigs receiving dietary CuSO4 and CuSO4+CS. Villus height and the ratio of villus height/crypt depth in duodenum, jejunum, and ileum were higher and crypt depth was lower in CS-Cu treated pigs than in pigs fed dietary CuSO4 or CuSO4+CS. An apparent decrease of ileal epithelial cell apoptosis in pigs fed CS-Cu diet was found. The activities of antioxidant enzymes were higher in pigs fed dietary CS-Cu than in those fed other diets. The results indicated that dietary CS-Cu showed better biological and physiological function in improving small intestinal morphology and reducing diarrhea incidence.

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