scholarly journals Effect of High Hydrostatic Pressure on Human Trabecular Bone Regarding Cell Death and Matrix Integrity

2021 ◽  
Vol 9 ◽  
Author(s):  
Janine Waletzko-Hellwig ◽  
Christopher Pohl ◽  
Janik Riese ◽  
Michael Schlosser ◽  
Michael Dau ◽  
...  
Keyword(s):  
Cell Death ◽  
Hydrostatic Pressure ◽  
Trabecular Bone ◽  
High Hydrostatic Pressure ◽  
Biological Effects ◽  
Donor Site ◽  
Biomechanical Properties ◽  
Point Analysis ◽  
Physical Processing ◽  
Biological Methods

The reconstruction of critical size bone defects is still clinically challenging. Even though the transplantation of autologous bone is used as gold standard, this therapy is accompanied by donor site morbidities as well as tissue limitations. The alternatively used allografts, which are devitalized due to thermal, chemical or physical processing, often lose their matrix integrity and have diminished biomechanical properties. High Hydrostatic Pressure (HHP) may represent a gentle alternative to already existing methods since HHP treated human osteoblasts undergo cell death and HHP treated bone cylinders maintain their mechanical properties. The aim of this study was to determine the biological effects caused by HHP treatment regarding protein/matrix integrity and type of cell death in trabecular bone cylinders. Therefore, different pressure protocols (250 and 300 MPa for 10, 20 and 30 min) and end point analysis such as quantification of DNA-fragmentation, gene expression, SDS-PAGE, FESEM analysis and histological staining were performed. While both protein and matrix integrity was preserved, molecular biological methods showed an apoptotic differentiation of cell death for lower pressures and shorter applications (250 MPa for 10 and 20 min) and necrotic differentiation for higher pressures and longer applications (300 MPa for 30 min). This study serves as a basis for further investigation as it shows that HHP successfully devitalizes trabecular bone cylinders.

scholarly journals Caspase-2 and oxidative stress underlie the immunogenic potential of high hydrostatic pressure-induced cancer cell death

2016 ◽  
Vol 6 (1) ◽  
pp. e1258505 ◽  
Author(s):  
Irena Moserova ◽  
Iva Truxova ◽  
Abhishek D. Garg ◽  
Jakub Tomala ◽  
Patrizia Agostinis ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Death ◽  
Hydrostatic Pressure ◽  
Cancer Cell ◽  
High Hydrostatic Pressure ◽  
Cancer Cell Death ◽  
Caspase 2 ◽  
And Oxidative Stress

Molecular characterization of immunogenic cell death triggered by the high hydrostatic pressure in human tumor cells.

2014 ◽  
Vol 32 (15_suppl) ◽  
pp. e14008-e14008
Author(s):  
Irena Moserova ◽  
Iva Truxova ◽  
Pierre-Francois Cartron ◽  
Jirina Bartunkova ◽  
Radek Spisek ◽  
...  
Keyword(s):  
Cell Death ◽  
Hydrostatic Pressure ◽  
Tumor Cells ◽  
Molecular Characterization ◽  
High Hydrostatic Pressure ◽  
Human Tumor ◽  
Immunogenic Cell Death ◽  
Human Tumor Cells

High hydrostatic pressure to induce immunogenic cell death in human tumor cells.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. 3076-3076
Author(s):  
Iva Truxova ◽  
Jitka Fucikova ◽  
Irena Moserova ◽  
Simona Partlova ◽  
Jirina Bartunkova ◽  
...  
Keyword(s):  
T Cells ◽  
Cell Death ◽  
Hydrostatic Pressure ◽  
Tumor Cells ◽  
Cell Lines ◽  
Primary Tumor ◽  
High Hydrostatic Pressure ◽  
Human Tumor ◽  
Immunogenic Cell Death ◽  
Human Tumor Cells

3076 Background: Recent studies have identified molecular events characteristic of immunogenic cell death. These include surface exposure of calreticulin, HSP70 and HSP90, release of intranuclear HMGB1 and secretion of ATP from dying cells. Several chemotherapeutic agents, including anthracyclins, oxaliplatin and bortezomib, and hypericin-based photodynamic therapy have been described to induce the immunogenic cell death in human tumor cells. We investigated the potential of high hydrostatic pressure (HHP) to induce immunogenic cell death in human tumor cells. Methods: Prostate and ovarian cancer cell lines and primary tumor cells were treated by HHP and we analyzed the kinetics of the expression of immunogenic cell death markers. HHP killed tumor cells expressing immunogenic cell death markers were tested for their ability to activate dendritic cells (DCs), to induce tumor specific T cells and regulatory T cells. Results: HHP induced rapid expression of HSP70, HSP90 and calreticulin on the cell surface of all tested cell lines and primary tumor cells. HHP also induced release of HMGB1 and ATP from treated cells. The kinetics of expression was similar to doxorubicin, HHP, however, induced 1.5-2 fold higher expression of HSP70, HSP90 and calreticulin. The interaction of DCs with HHP-treated tumor cells led to the faster rate of phagocytosis, significant upregulation of CD83, CD86 and HLA-DR and release of IL-6, IL-12p70 and TNFα. The ability of HHP-killed tumor cells to promote DCs maturation was cell contact dependent. DCs pulsed with tumor cells killed by HHP induced high numbers of tumor-specific CD4+ and CD8+IFN-g-producing T cells even in the absence of additional maturation stimulus. DCs pulsed with HHP treated tumor cells also induced the lowest number of regulatory T cells among the tested conditions. Cells treated by HHP can by cryopreserved in liquid nitrogen and retain their immunogenic properties upon thawing thus allowing for their convenient use in the manufacturing of cancer immunotherapy products. Conclusions: High hydrostatic pressure is a reliable and very potent inducer of immunogenic cell death in the wide range of human tumor cell lines and primary tumor cells.

Induction of tumor cell death by high hydrostatic pressure as a novel supporting technique in orthopedic surgery

2003 ◽  
Author(s):  
Peter Diehl ◽  
Manfred Schmitt ◽  
Gerrit Blümelhuber ◽  
Benjamin Frey ◽  
Susanne Van Laak ◽  
...  
Keyword(s):  
Cell Death ◽  
Hydrostatic Pressure ◽  
Tumor Cell ◽  
Orthopedic Surgery ◽  
High Hydrostatic Pressure ◽  
Tumor Cell Death

scholarly journals Improved Biotransformation of Platycoside E into Deapiose-Xylosylated Platycodin D by Cytolase PCL5 under High Hydrostatic Pressure

2021 ◽  
Vol 11 (22) ◽  
pp. 10623
Author(s):  
Kyung-Chul Shin ◽  
Min-Ju Seo ◽  
Yu Jin Oh ◽  
Dae Wook Kim ◽  
Chae Sun Na ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
Atmospheric Pressure ◽  
High Hydrostatic Pressure ◽  
Biological Effects ◽  
Potential Method ◽  
Optimal Temperature ◽  
Platycodin D

Platycosides are the functional saponins present in balloon flowers that exert diverse biological effects, and which can be further improved by their deglycosylation. Deapiose-xylosylated platycodin D, which is absent in balloon flowers, can be generated only by cytolase PCL5 by acting on platycoside E. To improve cytolase PCL5-catalyzed production of deapiose-xylosylated platycodin D from platycoside E, we explored the use of high hydrostatic pressure (HHP). At an HHP of 150 MPa, the optimal temperature of cytolase PCL5 activity for converting platycoside E into deapiose-xylosylated platycodin D shifted from 50 to 55 °C, and increased the activity and stability of the enzyme by 5- and 4.9-fold, respectively. Under HHP, the enzyme completely converted 1 mM platycoside E into deapiose-xylosylated platycodin D within 4 h, with a 3.75-fold higher productivity than that under atmospheric pressure. Our results suggest that the application of HHP is a potential method for the economical production of platycosides and enzyme-catalyzed biotransformation of functional saponins.

Cells Under Pressure – Treatment of Eukaryotic Cells with High Hydrostatic Pressure, from Physiologic Aspects to Pressure Induced Cell Death

2008 ◽  
Vol 15 (23) ◽  
pp. 2329-2336 ◽  
Author(s):  
Benjamin Frey ◽  
Christina Janko ◽  
Nina Ebel ◽  
Silke Meister ◽  
Eberhard Schlucker ◽  
...  
Keyword(s):  
Cell Death ◽  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Eukaryotic Cells ◽  
Pressure Treatment

scholarly journals High Hydrostatic Pressure Therapy Annihilates Squamous Cell Carcinoma in a Murine Model

2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Toshihito Mitsui ◽  
Naoki Morimoto ◽  
Atsushi Mahara ◽  
Sharon Claudia Notodihardjo ◽  
Tien Minh Le ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Hydrostatic Pressure ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
High Hydrostatic Pressure ◽  
Donor Site ◽  
Congenital Melanocytic Nevus ◽  
Pressure Therapy

Cutaneous squamous cell carcinoma (cSCC) is one of the most common skin cancers. In the treatment of cSCC, it is necessary to remove it completely, and reconstructive surgery, such as a skin graft or a local or free flap, will be required, depending on the size, with donor-site morbidity posing a burden to the patient. The high hydrostatic pressure (HHP) technique has been developed as a physical method of decellularizing various tissues. We previously reported that HHP at 200 MPa for 10 min could inactivate all cells in the giant congenital melanocytic nevus, and we have already started a clinical trial using this technique. In the present study, we explored the critical pressurization condition for annihilating cSCC cells in vitro and confirmed that this condition could also annihilate cSCC in vivo. We prepared 5 pressurization conditions in this study (150, 160, 170, 180, and 190 MPa for 10 min) and confirmed that cSCC cells were killed by pressurization at ≥160 MPa for 10 min. In the in vivo study, the cSCC cells inactivated by HHP at 200 MPa for 10 min were unable to proliferate after injection into the intradermal space of mice, and transplanted cSCC tissues that had been inactivated by HHP showed a decreased weight at 5 weeks after implantation. These results suggested that HHP at 200 MPa for 10 min was able to annihilate SCC, so HHP technology may be a novel treatment of skin cancer.

scholarly journals Devitalizing Effect of High Hydrostatic Pressure on Human Cells—Influence on Cell Death in Osteoblasts and Chondrocytes

2020 ◽  
Vol 21 (11) ◽  
pp. 3836
Author(s):  
Janine Waletzko ◽  
Michael Dau ◽  
Anika Seyfarth ◽  
Armin Springer ◽  
Marcus Frank ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cell Death ◽  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Cell Damage ◽  
Water Soluble ◽  
Cartilage Tissue ◽  
Initial Assessment ◽  
Tetrazolium Salt ◽  
Tissue Samples

Chemical and physical processing of allografts is associated with a significant reduction in biomechanics. Therefore, treatment of tissue with high hydrostatic pressure (HHP) offers the possibility to devitalize tissue gently without changing biomechanical properties. To obtain an initial assessment of the effectiveness of HHP treatment, human osteoblasts and chondrocytes were treated with different HHPs (100–150 MPa, 250–300 MPa, 450–500 MPa). Devitalization efficiency was determined by analyzing the metabolic activity via WST-1(water-soluble tetrazolium salt) assay. The type of cell death was detected with an apoptosis/necrosis ELISA (enzyme-linked immune sorbent assay) and flow cytometry. Field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) were carried out to detect the degree of cell destruction. After HHP treatment, the metabolic activities of both cell types decreased, whereas HHP of 250 MPa and higher resulted in metabolic inactivation. Further, the highest HHP range induced mostly necrosis while the lower HHP ranges induced apoptosis and necrosis equally. FESEM and TEM analyses of treated osteoblasts revealed pressure-dependent cell damage. In the present study, it could be proven that a pressure range of 250–300 MPa can be used for cell devitalization. However, in order to treat bone and cartilage tissue gently with HHP, the results of our cell experiments must be verified for tissue samples in future studies.

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