scholarly journals Improved Biotransformation of Platycoside E into Deapiose-Xylosylated Platycodin D by Cytolase PCL5 under High Hydrostatic Pressure

2021 ◽  
Vol 11 (22) ◽  
pp. 10623
Author(s):  
Kyung-Chul Shin ◽  
Min-Ju Seo ◽  
Yu Jin Oh ◽  
Dae Wook Kim ◽  
Chae Sun Na ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
Atmospheric Pressure ◽  
High Hydrostatic Pressure ◽  
Biological Effects ◽  
Potential Method ◽  
Optimal Temperature ◽  
Platycodin D

Platycosides are the functional saponins present in balloon flowers that exert diverse biological effects, and which can be further improved by their deglycosylation. Deapiose-xylosylated platycodin D, which is absent in balloon flowers, can be generated only by cytolase PCL5 by acting on platycoside E. To improve cytolase PCL5-catalyzed production of deapiose-xylosylated platycodin D from platycoside E, we explored the use of high hydrostatic pressure (HHP). At an HHP of 150 MPa, the optimal temperature of cytolase PCL5 activity for converting platycoside E into deapiose-xylosylated platycodin D shifted from 50 to 55 °C, and increased the activity and stability of the enzyme by 5- and 4.9-fold, respectively. Under HHP, the enzyme completely converted 1 mM platycoside E into deapiose-xylosylated platycodin D within 4 h, with a 3.75-fold higher productivity than that under atmospheric pressure. Our results suggest that the application of HHP is a potential method for the economical production of platycosides and enzyme-catalyzed biotransformation of functional saponins.

Improvement in the efficiency of oxidative phosphorylation in the freshwater eel acclimated to 10.1 MPa hydrostatic pressure

2000 ◽  
Vol 203 (19) ◽  
pp. 3019-3023
Author(s):  
M. Theron ◽  
F. Guerrero ◽  
P. Sebert
Keyword(s):  
Hydrostatic Pressure ◽  
Oxidative Phosphorylation ◽  
Atmospheric Pressure ◽  
High Hydrostatic Pressure ◽  
Anguilla Anguilla ◽  
Respiratory Chain Complexes ◽  
Complex Iv ◽  
Control Value ◽  
Chain Complexes ◽  
Freshwater Eel

Previous studies have suggested that the efficiency of oxidative phosphorylation in the freshwater eel (Anguilla anguilla) is increased after acclimation to high hydrostatic pressure. Analysis at atmospheric pressure of the respiratory chain complexes showed that, after 21 days at 10.1 MPa, the activity of complex II was decreased to approximately 50 % (P<0.01) of the control value and that cytochrome c oxidase (complex IV) activity was significantly increased to 149 % of the control value (P<0.05). ADP/O ratios calculated from mitochondrial respiration measurements were significantly increased after acclimation to high hydrostatic pressure (2.87 versus 2.52, P<0.001) when measured in the presence of pyruvate plus malate at atmospheric pressure. These results clearly show an increased oxidative phosphorylation efficiency in response to high-pressure acclimation.

Effect of High Hydrostatic Pressure on the Permeability of Elastomers to Water

1966 ◽  
Vol 39 (4) ◽  
pp. 1298-1307 ◽  
Author(s):  
Alexander Lebovits
Keyword(s):  
Hydrostatic Pressure ◽  
Thermodynamic Analysis ◽  
Atmospheric Pressure ◽  
High Hydrostatic Pressure ◽  
Polymeric Materials ◽  
Butyl Rubber ◽  
Pressurized Water ◽  
Simple Method ◽  
Increased Pressure

Abstract The permeability of butyl rubber to pressurized water was measured at 10,000 psi hydrostatic pressure using a relatively simple method which consisted of constructing pouches from rubber, filling these with desiccant and exposing them to pressurized water. At this high hydrostatic pressure the permeability was found to be smaller than at atmospheric pressure which suggests the suitability of butyl rubber for the construction of hydrophone boots used at great depths. Similar results found by other investigators for other polymeric materials are cited. A thermodynamic analysis of the process of permeation by activated diffusion was made and a mechanism was discussed which explains the decrease in permeability of butyl rubber to water with increased pressure.

scholarly journals Effect of High Hydrostatic Pressure on Human Trabecular Bone Regarding Cell Death and Matrix Integrity

2021 ◽  
Vol 9 ◽  
Author(s):  
Janine Waletzko-Hellwig ◽  
Christopher Pohl ◽  
Janik Riese ◽  
Michael Schlosser ◽  
Michael Dau ◽  
...  
Keyword(s):  
Cell Death ◽  
Hydrostatic Pressure ◽  
Trabecular Bone ◽  
High Hydrostatic Pressure ◽  
Biological Effects ◽  
Donor Site ◽  
Biomechanical Properties ◽  
Point Analysis ◽  
Physical Processing ◽  
Biological Methods

The reconstruction of critical size bone defects is still clinically challenging. Even though the transplantation of autologous bone is used as gold standard, this therapy is accompanied by donor site morbidities as well as tissue limitations. The alternatively used allografts, which are devitalized due to thermal, chemical or physical processing, often lose their matrix integrity and have diminished biomechanical properties. High Hydrostatic Pressure (HHP) may represent a gentle alternative to already existing methods since HHP treated human osteoblasts undergo cell death and HHP treated bone cylinders maintain their mechanical properties. The aim of this study was to determine the biological effects caused by HHP treatment regarding protein/matrix integrity and type of cell death in trabecular bone cylinders. Therefore, different pressure protocols (250 and 300 MPa for 10, 20 and 30 min) and end point analysis such as quantification of DNA-fragmentation, gene expression, SDS-PAGE, FESEM analysis and histological staining were performed. While both protein and matrix integrity was preserved, molecular biological methods showed an apoptotic differentiation of cell death for lower pressures and shorter applications (250 MPa for 10 and 20 min) and necrotic differentiation for higher pressures and longer applications (300 MPa for 30 min). This study serves as a basis for further investigation as it shows that HHP successfully devitalizes trabecular bone cylinders.

scholarly journals Improved production of deglucosylated platycodin D from saponins from balloon flower leaf by a food-grade enzyme using high hydrostatic pressure

Heliyon ◽  
2021 ◽  
Vol 7 (10) ◽  
pp. e08104
Author(s):  
Kyung-Chul Shin ◽  
Dae Wook Kim ◽  
Yu Jin Oh ◽  
Min-Ju Seo ◽  
Chae Sun Na ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Food Grade ◽  
Platycodin D

scholarly journals Quality Evaluation of Rice Treated by High Hydrostatic Pressure and Atmospheric Pressure Plasma

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Ji Hae Lee ◽  
Koan Sik Woo ◽  
Cheorun Jo ◽  
Heon Sang Jeong ◽  
Seuk Ki Lee ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
Physicochemical Properties ◽  
Atmospheric Pressure ◽  
High Hydrostatic Pressure ◽  
Quality Evaluation ◽  
Microbial Population ◽  
Atmospheric Pressure Plasma ◽  
Sugar Composition ◽  
Pressure Plasma ◽  
Intended Use

This study applied high hydrostatic pressure (HHP) and atmospheric pressure plasma (APP) treatments to rice and examined the effects of the treatments on the microbial contamination and physicochemical properties. The microbial population was 100% sterilized by HHP and reduced by up to 34% by APP. Color a values were increased by up to 285% and 33% in HHP and APP, respectively. HHP increased fructose (∼8,256%) but decreased glucose, sucrose, and maltose (∼97%, −100%, and −93%, respectively). APP only mildly modified sugar composition compared with HHP. Retrogradation factors were not changed remarkably by HHP or APP. In conclusion, HHP sterilized microorganisms, but the sterilization was accompanied by high modifications to color and sugar composition. APP had a lesser effect on the microbial population, but it only mildly changed the physicochemical properties of the rice. Therefore, application of either HHP or APP could be considered depending on the intended use of the rice.

scholarly journals Microstructure Evolution During Normal Grain Growth Under High Pressure in 2-D Aluminium Foils

1999 ◽  
Vol 32 (1-4) ◽  
pp. 175-185 ◽  
Author(s):  
V. Sursaeva ◽  
S. Protasova ◽  
W. Lojkowski ◽  
J. Jun
Keyword(s):  
Growth Rate ◽  
High Pressure ◽  
Hydrostatic Pressure ◽  
Grain Growth ◽  
Microstructure Evolution ◽  
Atmospheric Pressure ◽  
High Hydrostatic Pressure ◽  
Activation Volume ◽  
Secondary Recrystallization ◽  
The Mean

We investigated the effect of high hydrostatic pressure on the normal grain growth in 2-D aluminium foils. The time dependence of the mean grain area was obtained. It was shown that normal grain growth takes place both at atmospheric pressure and under high hydrostatic pressure. The grain growth rate decreases by a factor 1.3 under high pressure. The activation volume for grain growth was 0.13 of the atomic volume. It was shown that high pressure strongly influences the ratio oflow angle boundaries and general boundaries at the early stages of secondary recrystallization.

Survival of mouse blastocysts after low-temperature preservation under high pressure

2004 ◽  
Vol 52 (4) ◽  
pp. 479-487 ◽  
Author(s):  
Cs. Pribenszky ◽  
M. Molnár ◽  
S. Cseh ◽  
L. Solti
Keyword(s):  
Phase Transition ◽  
Hydrostatic Pressure ◽  
Low Temperature ◽  
High Hydrostatic Pressure ◽  
Room Temperature ◽  
Freezing Point ◽  
Significant Loss ◽  
Embryo Cryopreservation ◽  
Subzero Temperatures ◽  
Survival Capacity

Cryoinjuries are almost inevitable during the freezing of embryos. The present study examines the possibility of using high hydrostatic pressure to reduce substantially the freezing point of the embryo-holding solution, in order to preserve embryos at subzero temperatures, thus avoiding all the disadvantages of freezing. The pressure of 210 MPa lowers the phase transition temperature of water to -21°C. According to the results of this study, embryos can survive in high hydrostatic pressure environment at room temperature; the time embryos spend under pressure without significant loss in their survival could be lengthened by gradual decompression. Pressurisation at 0°C significantly reduced the survival capacity of the embryos; gradual decompression had no beneficial effect on survival at that stage. Based on the findings, the use of the phenomena is not applicable in this form, since pressure and low temperature together proved to be lethal to the embryos in these experiments. The application of hydrostatic pressure in embryo cryopreservation requires more detailed research, although the experience gained in this study can be applied usefully in different circumstances.

Effect of High Hydrostatic Pressure on Endothelial Lipase Expression*

2010 ◽  
Vol 37 (6) ◽  
pp. 641-645 ◽  
Author(s):  
Can-Xin XU ◽  
Chun WANG ◽  
Bing-Yang ZHU ◽  
Zhi-Ping GAO ◽  
Di-Xian LUO ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Endothelial Lipase ◽  
Lipase Expression
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