scholarly journals Improvement of Background Solution for Optically Induced Dielectrophoresis-Based Cell Manipulation in a Microfluidic System

2021 ◽  
Vol 9 ◽  
Author(s):  
Po-Yu Chu ◽  
Chia-Hsun Hsieh ◽  
Chih-Yu Chen ◽  
Min-Hsien Wu
Keyword(s):  
Cell Viability ◽  
Sucrose Solution ◽  
Operation Time ◽  
Microfluidic System ◽  
Cell Manipulation ◽  
Fundamental Information ◽  
Sucrose Solutions ◽  
Solution Conductivity ◽  
And Performance ◽  
Optically Induced

Optically induced dielectrophoresis (ODEP) is effective for cell manipulation. However, its utilization has been limited by the requirement of solution with low conductivity. This issue has been ignored in ODEP-relevant studies. To address this issue, this study aims to investigate to what extent the cell viability and performance of ODEP-based cell manipulation are affected by low conductivity conditions. Additionally, this study aims to modify sucrose solutions to reduce the impacts caused by low-conductivity solutions. Results revealed the use of sucrose solution in ODEP operation could significantly reduce the viability of the manipulated cells by 9.1 and 38.5% after 2- and 4-h incubation, respectively. Prolonged operation time (e.g., 4 h) in sucrose solution could lead to significantly inferior performance of cell manipulation, including 47.2% reduction of ODEP manipulation velocity and 44.4% loss of the cells manipulatable by ODEP. The key finding of this study is that the use of bovine serum albumin (BSA)-supplemented sucrose solution (conductivity: 25–50 μS cm−1) might significantly increase the cell viability by 10.9–14.8% compared with that in sucrose solution after 4 h incubation. Moreover, the ODEP manipulation velocity of cells in the BSA-supplemented sucrose solution (conductivity: 25 μS cm−1) was comparable to that in sucrose solution during 4-h incubation. More importantly, compared with sucrose solution, the use of BSA-supplemented sucrose solution (conductivity: 25–50 μS cm−1) contributed high percentage (80.4–93.5%) of the cells manipulatable by ODEP during 4-h incubation. Overall, this study has provided some fundamental information relevant to the improvement of background solutions for ODEP-based cell manipulation.

scholarly journals The Effect of Optically Induced Dielectrophoresis (ODEP)-Based Cell Manipulation in a Microfluidic System on the Properties of Biological Cells

Biosensors ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 65
Author(s):  
Po-Yu Chu ◽  
Chia-Hsun Hsieh ◽  
Chien-Ru Lin ◽  
Min-Hsien Wu
Keyword(s):  
Gene Expression ◽  
Cell Viability ◽  
Cancer Cells ◽  
Cell Types ◽  
Microfluidic System ◽  
Cell Manipulation ◽  
Biological Cells ◽  
Fundamental Issue ◽  
Experimental Conditions ◽  
Optically Induced

Cell manipulation using optically induced dielectrophoresis (ODEP) in microfluidic systems has attracted the interest of scientists due to its simplicity. Although this technique has been successfully demonstrated for various applications, one fundamental issue has to be addressed—Whether, the ODEP field affects the native properties of cells. To address this issue, we explored the effect of ODEP electrical conditions on cellular properties. Within the experimental conditions tested, the ODEP-based cell manipulation with the largest velocity occurred at 10 Vpp and 1 MHz, for the two cancer cell types explored. Under this operating condition, however, the cell viability of cancer cells was significantly affected (e.g., 70.5 ± 10.0% and 50.6 ± 9.2% reduction for the PC-3 and SK-BR-3 cancer cells, respectively). Conversely, the exposure of cancer cells to the ODEP electrical conditions of 7–10 Vpp and 3–5 MHz did not significantly alter the cell viability, cell metabolic activity, and the EpCAM, VIM, and ABCC1 gene expression of cancer cells. Overall, this study fundamentally investigated the effect of ODEP electrical conditions on the cellular properties of cancer cells. The information obtained is crucially important for the utilization of ODEP-based cell manipulation in a microscale system for various applications.

Closed-loop feedback control of microfluidic cell manipulation via deep-learning integrated sensor networks

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Ningquan Wang ◽  
Ruxiu Liu ◽  
Norh Asmare ◽  
Chia-Heng Chu ◽  
Ozgun Civelekoglu ◽  
...  
Keyword(s):  
Deep Learning ◽  
Sensor Networks ◽  
Feedback Control ◽  
Closed Loop ◽  
Microfluidic System ◽  
Cell Manipulation ◽  
Integrated Sensor ◽  
Loop Feedback ◽  
Closed Loop Feedback ◽  
On Chip

An adaptive microfluidic system changing its operational state in real-time based on cell measurements through an on-chip electrical sensor network.

scholarly journals Sucrose solution as a new viscous test fluid with tunable viscosities up to 2 Pas for micromixing characterization by the Villermaux–Dushman reaction

2021 ◽  
Author(s):  
Elias Arian ◽  
Werner Pauer
Keyword(s):  
Sucrose Solution ◽  
Viscous Medium ◽  
Stirred Tank ◽  
Test Fluid ◽  
Tank Reactor ◽  
Experimental Implementation ◽  
Sucrose Solutions ◽  
Viscous Solution ◽  
First Time ◽  
Dushman Reaction

AbstractFor the first time, micromixing characterization for the Villermaux–Dushman reaction could be performed with a non-reactive viscous medium at viscosities up to 2 Pas. As viscous medium, sucrose solution was used with the benefit of being a Newtonian fluid with tuneable viscosity. Due to the higher viscosities in comparison to established media for micromixing investigations, a new protocol for the experimental implementation was developed. Micromixing experiments were conducted and the applicability of viscous sucrose solutions was proven in a stirred tank reactor. Major challenges in characterizing micromixing efficiency in high viscous solution were consolidated.

Taste Responsiveness of Spider Monkeys to Dietary Ethanol

2019 ◽  
Vol 44 (8) ◽  
pp. 631-638 ◽  
Author(s):  
Daniel Dausch Ibañez ◽  
Laura Teresa Hernandez Salazar ◽  
Matthias Laska
Keyword(s):  
Short Duration ◽  
Sucrose Solution ◽  
Preference Test ◽  
Taste Preference ◽  
Ateles Geoffroyi ◽  
Spider Monkeys ◽  
Taste Preferences ◽  
Additional Source ◽  
Ethanol Mixture ◽  
Sucrose Solutions

Abstract Recent studies suggest that frugivorous primates might display a preference for the ethanol produced by microbia in overripe, fermenting fruit as an additional source of calories. We, therefore, assessed the taste responsiveness of 8 spider monkeys (Ateles geoffroyi) to the range of ethanol concentrations found in overripe, fermenting fruit (0.05–3.0%) and determined taste preference thresholds as well as relative taste preferences for ethanol presented in sucrose solutions and in fruit matrices, respectively. Using a 2-bottle preference test of short duration (1 min), we found that spider monkeys are able to detect ethanol concentrations as low as 0.5%, that they prefer ethanol concentrations up to 3% over water, and that they prefer sucrose solutions and pureed fruit spiked with ethanol over equimolar sucrose solutions and pureed fruit without ethanol. However, when presented with an ethanol-spiked sucrose solution and a higher-concentrated sucrose solution without ethanol, the animals clearly preferred the latter, even when the sucrose–ethanol mixture contained 3 times more calories. These results demonstrate that spider monkeys are more sensitive to the taste of ethanol than rats and humans and that they prefer ecologically relevant suprathreshold concentrations of ethanol over water. Tests with sucrose solutions and pureed fruits that were either spiked with ethanol or not suggest that sweetness may be more important for the preferences displayed by the spider monkeys than the calories provided by ethanol. The present results, therefore, do not support the notion that dietary ethanol might be used by frugivorous primates as a supplemental source of calories.

Adaptations to Intraperitoneal Sucrose Solutions in the Rat

1956 ◽  
Vol 184 (2) ◽  
pp. 390-395
Author(s):  
P. F. Iampietro
Keyword(s):  
Peritoneal Cavity ◽  
Urine Output ◽  
Sucrose Solution ◽  
Tubular Reabsorption ◽  
Sucrose Solutions

Rats injected intraperitoneally with sucrose solution (1.5 m) two or more times at weekly intervals adapt by increasing the concentration of sucrose in the urine at 1–3 hours and by increasing urine output at the end of 6 hours. The adaptation appears within 2 days after the first injection and disappears after 13 days of no injections. There is no adaptation in absorption from the peritoneal cavity. The adaptation in excretion may therefore be the result of reduced tubular reabsorption of sucrose. Adaptations were observed also in drinking responses and in survival when toxic doses were administered.

Design and Performance of a Microfluidic Particle Sorting Device

2011 ◽  
Vol 52-54 ◽  
pp. 668-673
Author(s):  
Jun Yang ◽  
Jing Yang ◽  
Qing He You ◽  
Ning Hu ◽  
Yong Li ◽  
...  
Keyword(s):  
Incident Light ◽  
Microfluidic System ◽  
Optic Fiber ◽  
Detection Zone ◽  
Severe Injuries ◽  
Electric Voltage ◽  
Particle Sorting ◽  
Electric Deflection ◽  
Optic Fibers ◽  
And Performance

A microfluidic chip device was developed for rapid and automatic particle sorting. The chip was made up of six individual layers. Each layer was used to implement different functions such as the sample loading, electric voltage loading, optic detection, cell sorting, and product extraction. Sheath flow was used to form single-row cells in order to let cells flow through the optic detection zone one by one. Optic fiber based detector could distinguish particles with different sizes. When a particle flowed through the light beam between two opposite optic fibers, it induced the dispersion of the incident light. The size of the particle was related with the strength of the scattering light. Thus, different particles could be distinguished. In the detection method, light path was simple and label was not required. Furthermore, optic fiber based detector was helpful to decrease the volume of the whole system. When desired particle was detected, it would be separated in the downstream by using an electric deflection method. Two types of particles with different sizes were sorted by using a prototype device. The result showed that these particles could be obviously distinguished. This microfluidic system could also be used to sort biological cells. Instantaneous electric field on the cells could prevent cells from severe injuries.

An Automated System for Controlling the Laminar Flow Interface in a Microfluidic System

2004 ◽  
Author(s):  
Brandon Kuczenski ◽  
Philip R. LeDuc ◽  
William C. Messner
Keyword(s):  
Laminar Flow ◽  
Feedback System ◽  
Microfluidic System ◽  
Automated System ◽  
Time Varying ◽  
Relative Flow Rate ◽  
Microfluidic Network ◽  
Piezoresistive Pressure Sensor ◽  
Pressure Feedback ◽  
And Performance

The interface between adjacent laminar flow streams in the output channel of a Y-shaped confluent microfluidic network is useful for investigating the response of individual living cells to steep chemical gradients. This paper reports the design and performance of an automated pressure-feedback system for accurately and rapidly changing the position of that interface. The device will be employed to investigate the dynamic response of cells to time-varying chemical stimulation. The system works by controlling the pressure difference between the two adjoining inputs of the microfluidic network, altering the relative flow rate of the laminar streams in the output microchannel. Continuity of incompressible fluids dictates that the plane of the interface between the two streams will move from side to side as the flow rates change. The sample-data control system samples a temperature-compensated monolithic piezoresistive pressure sensor at 1 kilohertz, allowing the control of high-bandwidth microfluidic systems. This automated system enables long-duration, high-precision experiments that involve time-varying parameters to be performed simply, rapidly, and inexpensively.

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