Beef tallow injection matrix for serial crystallography

Serial crystallography (SX) enables the visualization of the time-resolved molecular dynamics of macromolecular structures at room temperature while minimizing radiation damage. In SX experiments, the delivery of a large number of crystals into an X-ray interaction point in a serial and stable manner is key. Sample delivery using viscous medium maintains the stable injection stream at low flow rates, markedly reducing sample consumption compared with that of a liquid jet injector and is widely applied in SX experiments with low repetition rates. As the sample properties and experimental environment can affect the stability of the injection stream of a viscous medium, it is important to develop sample delivery media with various characteristics to optimize the experimental environment. In this study, a beef tallow injection matrix possessing a higher melting temperature than previously reported fat-based shortening and lard media was introduced as a sample delivery medium and applied to SX. Beef tallow was prepared by heat treating fats from cattle, followed by the removal of soluble impurities from the extract by phase separation. Beef tallow exhibited a very stable injection stream at room temperature and a flow rate of < 10 nL/min. The room-temperature structures of lysozyme and glucose isomerase embedded in beef tallow were successfully determined at 1.55 and 1.60 Å, respectively. The background scattering of beef tallow was higher than that of previously reported fat-based shortening and lard media but negligible for data processing. In conclusion, the beef tallow matrix can be employed for sample delivery in SX experiments conducted at temperatures exceeding room temperature.

The dynamics of bouncing, partially coalescing, liquid metal droplets in a viscous medium

Planar partial coalescence is a phenomenon in which a droplet at a free surface or interface between two fluids coalesces into the plane surface producing a smaller droplet rather than coalescing completely. This smaller, ‘daughter’ droplet will be driven towards the interface by gravity and capillary forces resulting in a cascade effect of progressively small daughter droplets until the Ohnesorge Number approaches $\sim$ 1 and the cascade terminates with a full coalescence event. This paper utilizes a room temperature liquid metal alloy composed of gallium, indium and tin to study partial coalescence in a viscous quiescent medium and observed bouncing of the coalescing droplets on the interface. We observed the event using high speed videography measuring effects such as the droplet to daughter droplet ratio, droplet velocities, droplet bounce heights and coefficients of restitution for the bouncing event. An existing model (Honey & Kavehpour, Phys. Rev. E, vol. 73, 2006) from our group was used, validated and expanded upon to include buoyancy effects to estimate the initial velocity of the droplet and we developed two new models for the droplet travel and maximum bounce height. The first utilizes the Stokes model for drag to moderate success while the second utilizes a model from Beard & Pruppacher (J. Atmos. Sci., vol. 26, 1969, pp. 1066–1072) and a fourth-order Runge–Kutta numerical integration scheme to predict the droplet velocity and position as functions of time. Additionally the coefficient of restitution was determined from the model using a shooting method technique in tandem with measured data to find a coefficient of restitution value of $A = 0.27 \pm 0.06$ . This ‘bouncing drop’ phenomenon continues in a quiescent viscous fluid to the sub-micron scale and was facilitated by the material properties of the liquid metal including the high density, moderate viscosity and particularly high interfacial tension.

LBM modeling of solid particle dynamics in a viscous medium

This article discusses the mathematical and computer modeling of single solid particle dynamics in a viscous medium. The results of the study were obtained using a 3D numerical algorithm implemented on the basis of the D3Q19 model of the lattice Boltzmann method (LBM). The moving «liquid-solid» interface is accounted for using an interpolated bounce back (IBB) scheme. The velocity of a solid particle motion and the trajectory of a particle at Re = 1,56 are obtained. The results are in good agreement with the experimental and numerical results of other authors.

Vibrations of Misaligned Rotor System with Hysteretic Friction Arising from Driveshaft–Stator Contact under Dispersed Viscous Fluid Influences

Dynamic analysis of a combination of misaligned rotors, the disturbance of the Cardan joint and the rotor–stator rubbing within a restricted clearance space in a viscous fluid is complex and can result in persistent vibration anomalies that are often misunderstood. It becomes increasingly important to gain some insights into how the transmission of coupled motion responds dynamically under a variety of conditions. This paper introduces an efficient simulation of the misaligned multi-degree-of-freedom rotor’s model, which was developed to predict the transient dynamic behaviours of a driveshaft deflection. The model accounts for tight clearance as a function of contact deformation according to nonlinear Hertzian contact theory. The paper also examines recent research by considering the influence of parameters such as eccentric masses, applied torques and flexible coupling joint perturbation introduced in the proposed rotor system. The simulation results indicated that the viscous fluid surrounding the driveshaft had sufficient torsional flexibility to dampen the rubbing impact to the driven shaft displacement. In addition, the torsional fluctuations of the flexible coupling abruptly increased, and then significantly impacted the vibration of the submerged driveshaft. Parametric studies involving the interconnected rotor models indicated that the effects of fluid on a close-bounds contact area can create partial disturbance reduction. The high rubbing contact is shown to be lost through the Hooke’s joints during power transmission. The speed-frequency spectrum maps provide valuable information on all the modelled excitations over the frequency of the twice-running speed resonance in a viscous medium. Further, nonlinear characteristics are reconstructed through orbit shapes and can be adopted in the condition monitoring of rotors in engineering practice.

FLYING SAUCER EXPERIMENTAL RESEARCH, AERODINAMICS UNEXPLORED PHENOMENON AND BIRD'S FLIGHT

As a result of experiments with the motion of asymmetrical vibrating bodies in a viscous medium, it was found that the generally accepted idea of such a principle of motion is not correct. In reality, the movement is in the opposite direction. A description of the experiments and the observed effect is given, as well as its interpretation.

A Novel Thrombectomy Device: An In Vitro Evaluation of a Prototype Catheter

Purpose: This prototype catheter is a newly-developed distal access catheter featuring a self-expanding, flexible, funnel-shaped tip. The purpose of its design is to reduce the risk of thrombus fragmentation during mechanical thrombectomy and improve first-pass recanalization (TICI 3). In this experimental setup, we preclinically evaluated the effectiveness and navigability of the new catheter. Methods: A vessel model was filled with a blood-like-viscous medium, and the image was projected with the corresponding vessel area by camera transmission to correspond to the conditions in an angiography. Thrombi from porcine blood were placed into the Arteria Carotis interna of the vascular model and subsequently mechanically thrombectomized with a stent retriever. In the first part, the prototype was compared to a standard distal-access-catheter without using an external catheter. (N = 20 for each catheter). In the second part the prototype was inserted through a guiding catheter (n=11) to determine the navigability performance. Results: In the first experimental series, mechanical thrombectomy was successful 19 out of 20 times (95% success rate) for the prototype catheter versus 15 out of 20 times (75% success rate) for the standard distal-access-catheter. In the second experimental series, the prototype catheter achieved first-pass recanalization 10 out of 11 times (91 % success rate) and 1 out of 11 times at second pass (9%). Conclusion: This series of experiments demonstrated higher first-pass recanalization rates for the newly-developed funnel-shaped prototype featuring a self-expanding tip in comparison to a cylindrical standard distal-access-catheter.

Stable sample delivery in a viscous medium via a polyimide-based single-channel microfluidic chip for serial crystallography

Serial crystallography (SX) provides room-temperature crystal structures with minimal radiation damage and facilitates the comprehension of molecular dynamics through time-resolved studies. In SX experiments, it is important to deliver a large number of crystal samples to the X-ray interaction point in a serial and stable manner. The advantage of crystal delivery in a viscous medium via a capillary is the ability to deliver all of the crystal samples to the X-ray interaction point at a low flow rate; however, the capillary often breaks during handling and high X-ray absorption can occur at low energy states. This study aimed to develop a stable system for sample delivery in a viscous medium via a polyimide-based single-channel microfluidic (PSM) chip for SX. Since this microfluidic chip comprises a polyimide film, it has high tensile strength and higher X-ray transmittance than a quartz capillary. The PSM chip was connected to a syringe containing the microcrystals embedded in viscous medium. The channel of the PSM chip was aligned to the X-ray path, and the viscous medium containing lysozyme crystals was stably delivered using a syringe pump at a flow rate of 100 nl min−1. Room-temperature lysozyme crystal structures were successfully determined at 1.85 Å resolution. This method would greatly facilitate sample delivery for SX experiments using synchrotron X-rays.

Multi-frequency passive and active microrheology with optical tweezers

Optical tweezers have attracted significant attention for microrheological applications, due to the possibility of investigating viscoelastic properties in vivo which are strongly related to the health status and development of biological specimens. In order to use optical tweezers as a microrheological tool, an exact force calibration in the complex system under investigation is required. One of the most promising techniques for optical tweezers calibration in a viscoelastic medium is the so-called active–passive calibration, which allows determining both the trap stiffness and microrheological properties of the medium with the least a-priori knowledge in comparison to the other methods. In this manuscript, we develop an optimization of the active–passive calibration technique performed with a sample stage driving, whose implementation is more straightforward with respect to standard laser driving where two different laser beams are required. We performed microrheological measurements over a broad frequency range in a few seconds implementing an accurate multi-frequency driving of the sample stage. The optical tweezers-based microrheometer was first validated by measuring water, and then exemplarily applied to more viscous medium and subsequently to a viscoelastic solution of methylcellulose in water. The described method paves the way to microrheological precision metrology in biological samples with high temporal- and spatial-resolution allowing for investigation of even short time-scale phenomena.