scholarly journals The RNA Methylation Modification 5-Methylcytosine Impacts Immunity Characteristics, Prognosis and Progression of Oral Squamous Cell Carcinoma by Bioinformatics Analysis

2021 ◽  
Vol 9 ◽  
Author(s):  
Li Gao ◽  
Ru Chen ◽  
Masahiro Sugimoto ◽  
Masanobu Mizuta ◽  
Lei Zhou ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Survival Rate ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Progression Free Survival ◽  
Pathological Process ◽  
Tumor Immune Microenvironment ◽  
Tumorigenesis And Progression ◽  
Tumor Mutational Burden

Disorders pertaining to 5-methylcytosine (m5C) modifications are involved in the pathological process of many diseases. However, the effect of m5C on the tumorigenesis and progression of oral squamous cell carcinoma (OSCC) remains unclear. In this study, we integrated the genomic and clinical data of 558 OSCC samples to comprehensively evaluate m5C modification patterns. Based on 16 m5C methylation regulators, two m5C modification clusters were identified with distinct tumor immune microenvironment (TIME) characteristics and prognosis in OSCC. We then performed weighted gene co-expression network analysis (WGCNA) to identify m5C modification cluster-related modules. Genes in the selected module were chosen to construct the m5Cscore scoring system for evaluating m5C modification pattern in individual OSCC patients. Patients with a high m5Cscore had higher immune, stromal, and ESTIMATE scores; lower tumor purity score; lower immune activity; and higher tumor mutational burden. The overall survival rate and progression-free survival rate were markedly worse and the tumor recurrence rate was higher in OSCC patients with a high m5Cscore. Furthermore, patients with oral leukoplakia who also had a high m5Cscore had a higher risk of deterioration to OSCC. This study demonstrated that m5C modification patterns might affect the TIME in OSCC. m5Cscore may provide a new approach for predicting the prognosis and progression of OSCC.

scholarly journals Tissue Mechanics and Expression of TROP2 in Oral Squamous Cell Carcinoma with Varying Differentiation

2020 ◽  
Author(s):  
Baoping Zhang ◽  
Shuting Gao ◽  
Yiting Li ◽  
Ruiping Li ◽  
Rui Cao ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Squamous Cell Carcinoma ◽  
Survival Rate ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Survival Curve ◽  
Tumor Development ◽  
Tissue Mechanics ◽  
Specific Marker

Abstract Background Trophoblast cell surface antigen 2 (TROP2) is overexpressed in many squamous cell carcinomas and contributes to tumor development and invasion. The correlation between the expression of TROP2 and occurrence and development of oral squamous cell carcinoma (OSCC) remains to be understood.Methods We have investigated the role of the specific marker TROP2 in OSCC patients using a combination of biophysics approaches. Analyzed 108 OSCC patient specimens with varying degrees of differentiation using hematoxylin and eosin staining and immunohistochemistry for TROP2 expression, along with Kaplan-Meier survival curve analysis and atomic force microscopy, to understand the morphology and mechanical properties of OSCC tissues.Results In total, 34% poor differentiation underwent high TROP expression. TROP2 as risk factor about patient age (OR = 0.437, P = 0.039), tumor size (OR = 13.148, P = 0.000), TNM stage (OR = 0.141, P = 0.000) during low survival rate. Average surface roughness of low, medium, and highly differentiated OSCC tissues were 448.9 ± 54.8, 792.7 ± 83.6, and 993.0 ± 104.3 nm, respectively. The Pearson coefficient revealed a negative correlation between stiffness and TROP2 expression (r=-0.84, P < 0.01).Conclusion TROP2 expression increased with the decrease in survival rate and negatively correlated with the various degrees of differentiation and tissue mechanics. Taken together, our findings demonstrate that TROP2 may be an indicator of OSCC differentiation that leads to the altered mechanical properties (e.g., stiffness) of OSCC tissues.

Does Microvascular free flap Reconstruction in oral Squamous cell Carcinoma Improve Patient Survival?

2008 ◽  
Vol 139 (6) ◽  
pp. 775-780 ◽  
Author(s):  
Claudio Marchetti ◽  
Angelo Pizzigallo ◽  
Riccardo Cipriani ◽  
Angelo Campobassi ◽  
Giovanni Badiali
Keyword(s):  
Squamous Cell Carcinoma ◽  
Survival Rate ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cox Regression ◽  
Survival Rates ◽  
Free Tissue Transfer ◽  
Microvascular Reconstruction ◽  
Improve Patient Survival

Objective We analyzed our experiences with microvascular reconstruction after oncologic resections for oral squamous cell carcinoma. Has microvascular surgery changed the survival rate of these patients? Design Retrospective study. Subjects and Methods Forty-two consecutive patients enrolled from March 1999 to December 2004. Follow-up time ranged from 1 to 94 months. Survival rates were evaluated by the Kaplan-Meier method and compared among different groups with the use of Cox regression. Results The actuarial 5-year survival rate was 41.9% (SD = 9.6%). Survival rates were also analyzed according to T, N, and stage. The survival was significantly related only to N, which showed a 72.4% increase in the risk related to the increase of one N stage. Conclusions A comparison between our study group and those of 3 previous similar studies would not provide definitive statistical evidence, but it could certainly suggest a trend. The comparison seems to support that microvascular free tissue transfer does not change the survival of these patients.

Correlation of human papillomavirus infection and clinical parameters with five-year survival in oral squamous cell carcinoma

2018 ◽  
Vol 132 (7) ◽  
pp. 628-635 ◽  
Author(s):  
S M Adnan Ali ◽  
M S Awan ◽  
S Atif ◽  
N Ali ◽  
Y Mirza
Keyword(s):  
Squamous Cell Carcinoma ◽  
Human Papillomavirus ◽  
Survival Rate ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Survival Time ◽  
Squamous Cell ◽  
Clinical Parameters ◽  
Care Hospital ◽  
Hpv Status

AbstractObjectiveThis study associated Human Papillomavirus (HPV) infection and other clinical parameters with five-year survival of oral squamous cell carcinoma patients at a tertiary care hospital in Karachi, Pakistan.MethodsA total of 140 patients diagnosed with oral squamous cell carcinoma were enlisted. HPV status and subtypes were established through polymerase chain reaction performed in a previously published study. Clinical data including five-year survival were obtained through institutional medical records.ResultsNinety-five patients (67.9 per cent) were positive for HPV. Of these, 85 patients were HPV 16 positive while 2 patients were HPV 18 positive. The mean survival time for HPV positive patients was 44.3 months, whereas survival time for HPV negative patients was 46.9 months. Univariate analysis showed that HPV status in oral squamous cell carcinoma was not a statistically significant factor in determining five-year survival rate (p = 0.386).ConclusionThere is a high prevalence of HPV positive oral squamous cell carcinoma in Pakistan; however, there is no difference in the five-year survival rate when compared to HPV negative oral squamous cell carcinoma.

scholarly journals Genomic and epigenetic signatures associated with survival rate in oral squamous cell carcinoma patients

2018 ◽  
Vol 9 (11) ◽  
pp. 1885-1895 ◽  
Author(s):  
Ilda Patrícia Ribeiro ◽  
Francisco Caramelo ◽  
Luísa Esteves ◽  
Camila Oliveira ◽  
Francisco Marques ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Survival Rate ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Epigenetic Signatures

scholarly journals Profiling of Mitochondrial DNA Heteroplasmy in a Prospective Oral Squamous Cell Carcinoma Study

Cancers ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 1933
Author(s):  
Liane Fendt ◽  
Federica Fazzini ◽  
Hansi Weissensteiner ◽  
Emanuel Bruckmoser ◽  
Sebastian Schönherr ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Mitochondrial Dna ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Coding Region ◽  
Protein Coding ◽  
Tumorigenesis And Progression ◽  
Impact On Survival ◽  
Cancer Tissues

While a shift in energy metabolism is essential to cancers, the knowledge about the involvement of the mitochondrial genome in tumorigenesis and progression in oral squamous cell carcinoma (OSCC) is still very limited. In this study, we evaluated 37 OSCC tumors and the corresponding benign mucosa tissue pairs by deep sequencing of the complete mitochondrial DNA (mtDNA). After extensive quality control, we identified 287 variants, 137 in tumor and 150 in benign samples exceeding the 1% threshold. Variant heteroplasmy levels were significantly increased in cancer compared to benign tissues (p = 0.0002). Furthermore, pairwise high heteroplasmy frequency difference variants (∆HF% > 20) with potential functional impact were increased in the cancer tissues (p = 0.024). Fourteen mutations were identified in the protein-coding region, out of which thirteen were detected in cancer and only one in benign tissue. After eight years of follow-up, the risk of mortality was higher for patients who harbored at least one ∆HF% > 20 variant in mtDNA protein-coding regions relative to those with no mutations (HR = 4.6, (95%CI = 1.3–17); p = 0.019 in primary tumor carriers). Haplogroup affiliation showed an impact on survival time, which however needs confirmation in a larger study. In conclusion, we observed a significantly higher accumulation of somatic mutations in the cancer tissues associated with a worse prognosis.

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