scholarly journals The relation between NEAT1 expression level and survival rate in patients with oral squamous cell carcinoma

2021 ◽  
Author(s):  
Nan-Chin Lin ◽  
Shih-Min Hsia ◽  
Tong-Hong Wang ◽  
Po-Jung Li ◽  
Yu-Hsin Tseng ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Survival Rate ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Expression Level

Expression, Proliferation and Apoptosis of miR‑92b in Oral Squamous Cell Carcinoma

2020 ◽  
Author(s):  
Yongzhi XU ◽  
Fang FANG ◽  
Jinghui WANG ◽  
Chunli ZHAO ◽  
Jingyang ZHAO ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Expression Level ◽  
Normal Tissues ◽  
Tumor Tissues ◽  
Proliferation And Apoptosis ◽  
Proliferation Ability ◽  
Rat Tissue

Background: Expression of miR‑92b in oral squamous cell carcinoma (OSCC) rat tissue and its effect on the OSCC CAL‑27 cells were investigated. Methods: The study was performed in Qingdao Stomatological Hospital, Qingdao, China on December 2018. Thirty Wistar rats were used to construct models of oral squamous cell carcinoma. CAL‑27 cells trascfected by Lipofectamine 2000 were divided into miR‑92b inhibitor, miR‑NC and blank groups. RT‑qPCR was used for the detection of the expression level of miR‑92b, and MTT and flow cytometry were carried out for the detection of the effect of miR‑92b on the proliferation and apoptosis of CAL‑27 cells, respectively. Results: The expression level of miR‑92b was significantly higher in tumor tissues than that in normal tissues (P<0.001). The miR‑92b inhibitor group had significantly lower proliferation ability but higher apoptosis rate of CAL‑27 cells than the miR‑NC and blank groups. After miR‑92b was downregulated by trans-fecting cells, the expression level of miR‑92b was significantly lower in the miR‑92b inhibitor group than that in the miR‑NC and blank groups. Conclusion: miR‑92b inhibitor can inhibit the proliferation of CAL‑27 cells and promote apoptosis, which provides certain references for clinical treatment. It is expected to be a potential target for treating OSCC.

scholarly journals Gene expression of one important microRNA in blood and tissue samples of oral squamous cell carcinoma

2021 ◽  
Author(s):  
Naghmeh Emami ◽  
Naghmeh Bahrami ◽  
Masoumeh Mirzaei ◽  
Abdolreza Mohamadnia
Keyword(s):  
Gene Expression ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Peripheral Blood ◽  
Expression Level ◽  
P Value ◽  
Healthy Individuals ◽  
Tissue Samples

Introduction: Oral Squamous Cell Carcinoma (OSCC) is one of the most common oral malignancies, which accounts for 80-90% of malignant neoplasms of the oral cavity. MicroRNAs (miRNAs) are small RNA molecules that regulate post-transcriptional gene expression by targeting mRNAs. Materials and Methods: In this case-control study, 40 patients with oral squamous cell carcinoma and 40 healthy individuals as control were studied. Blood samples were collected from both groups. Also, 30 cancer tissue samples and 30 healthy tissue samples were prepared and evaluated. RNA was extracted from collected peripheral blood and tissue samples and evaluated for the expression level of miR-494 via real-time PCR technique. P. value values<0.05 were considered statistically significant. Results: The expression level of miR-494 in serum (peripheral blood) of patients with oral squa- mous cell carcinoma increased by 1.12 fold (P-value<0.001) compared with healthy individuals. Also, the expression level of miR-494 in samples of oral squamous cell carcinoma infected tissue showed a 1.28-fold increase compared to healthy tissue. Conclusion: The results of this study indicate an increase in the expression level (up-regula- tion) of miR-494 in oral squamous cell carcinoma. This biomarker can be used in screening and early detection of oral squamous cell carcinoma.

scholarly journals KRT84 is a potential tumor suppressor and good prognosis signature of oral squamous cell carcinoma

2020 ◽  
Vol 40 (4) ◽  
Author(s):  
Yi Liu ◽  
Ronghua Li ◽  
Gang Ren
Keyword(s):  
Squamous Cell Carcinoma ◽  
Tumor Suppressor ◽  
Oral Squamous Cell Carcinoma ◽  
Western Blot ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Expression Level ◽  
Protein Levels ◽  
Multiple Factors ◽  
Presentation Pathway

Abstract Aims: Oral squamous cell carcinoma (OSCC) is a common oral cancer; however, current therapeutic approaches still show limited efficacy. Our research aims to explore effective biomarkers related to OSCC. Main methods: Gene expression profiles of paired OSCC tumor and paracancerous samples from The Cancer Genome Atlas (TCGA) were analyzed. mRNA and protein levels of KRT84 in OSCC cell line HSC-3 were measured by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot. KRT84 protein levels in OSCC tumor samples of different stages were determined by immunohistochemistry. Overall survival (OS) of OSCC samples was evaluated and association of multiple factors with OS was assessed. Key findings: Compared with paracancerous samples, 4642 DEGs were identified in OSCC tumor samples. Among them, KRT84 expression level in OSCC tumor tissues was obviously decreased, which was validated in HSC-3 cells. KRT84 expression level showed decreasing tendency with the increase of tumor grade and stage. Patients with low KRT84 expression level had inferior OS independently of multiple factors. Besides, antigen processing and presentation pathway were significantly activated in OSCC samples with high KRT84 expression. Elevated KRT84 mRNA as well as protein levels were confirmed by RT-qPCR and Western blot in OSCC and normal cell lines, and immunohistochemistry in OSCC tumor and paracancerous tissues. Significance: Our study suggests KRT84 as a tumor suppressor and good prognostic indicator for OSCC, which might be significant for OSCC diagnosis and treatment.

scholarly journals Tissue Mechanics and Expression of TROP2 in Oral Squamous Cell Carcinoma with Varying Differentiation

2020 ◽  
Author(s):  
Baoping Zhang ◽  
Shuting Gao ◽  
Yiting Li ◽  
Ruiping Li ◽  
Rui Cao ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Squamous Cell Carcinoma ◽  
Survival Rate ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Survival Curve ◽  
Tumor Development ◽  
Tissue Mechanics ◽  
Specific Marker

Abstract Background Trophoblast cell surface antigen 2 (TROP2) is overexpressed in many squamous cell carcinomas and contributes to tumor development and invasion. The correlation between the expression of TROP2 and occurrence and development of oral squamous cell carcinoma (OSCC) remains to be understood.Methods We have investigated the role of the specific marker TROP2 in OSCC patients using a combination of biophysics approaches. Analyzed 108 OSCC patient specimens with varying degrees of differentiation using hematoxylin and eosin staining and immunohistochemistry for TROP2 expression, along with Kaplan-Meier survival curve analysis and atomic force microscopy, to understand the morphology and mechanical properties of OSCC tissues.Results In total, 34% poor differentiation underwent high TROP expression. TROP2 as risk factor about patient age (OR = 0.437, P = 0.039), tumor size (OR = 13.148, P = 0.000), TNM stage (OR = 0.141, P = 0.000) during low survival rate. Average surface roughness of low, medium, and highly differentiated OSCC tissues were 448.9 ± 54.8, 792.7 ± 83.6, and 993.0 ± 104.3 nm, respectively. The Pearson coefficient revealed a negative correlation between stiffness and TROP2 expression (r=-0.84, P < 0.01).Conclusion TROP2 expression increased with the decrease in survival rate and negatively correlated with the various degrees of differentiation and tissue mechanics. Taken together, our findings demonstrate that TROP2 may be an indicator of OSCC differentiation that leads to the altered mechanical properties (e.g., stiffness) of OSCC tissues.

Does Microvascular free flap Reconstruction in oral Squamous cell Carcinoma Improve Patient Survival?

2008 ◽  
Vol 139 (6) ◽  
pp. 775-780 ◽  
Author(s):  
Claudio Marchetti ◽  
Angelo Pizzigallo ◽  
Riccardo Cipriani ◽  
Angelo Campobassi ◽  
Giovanni Badiali
Keyword(s):  
Squamous Cell Carcinoma ◽  
Survival Rate ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cox Regression ◽  
Survival Rates ◽  
Free Tissue Transfer ◽  
Microvascular Reconstruction ◽  
Improve Patient Survival

Objective We analyzed our experiences with microvascular reconstruction after oncologic resections for oral squamous cell carcinoma. Has microvascular surgery changed the survival rate of these patients? Design Retrospective study. Subjects and Methods Forty-two consecutive patients enrolled from March 1999 to December 2004. Follow-up time ranged from 1 to 94 months. Survival rates were evaluated by the Kaplan-Meier method and compared among different groups with the use of Cox regression. Results The actuarial 5-year survival rate was 41.9% (SD = 9.6%). Survival rates were also analyzed according to T, N, and stage. The survival was significantly related only to N, which showed a 72.4% increase in the risk related to the increase of one N stage. Conclusions A comparison between our study group and those of 3 previous similar studies would not provide definitive statistical evidence, but it could certainly suggest a trend. The comparison seems to support that microvascular free tissue transfer does not change the survival of these patients.

Correlation of human papillomavirus infection and clinical parameters with five-year survival in oral squamous cell carcinoma

2018 ◽  
Vol 132 (7) ◽  
pp. 628-635 ◽  
Author(s):  
S M Adnan Ali ◽  
M S Awan ◽  
S Atif ◽  
N Ali ◽  
Y Mirza
Keyword(s):  
Squamous Cell Carcinoma ◽  
Human Papillomavirus ◽  
Survival Rate ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Survival Time ◽  
Squamous Cell ◽  
Clinical Parameters ◽  
Care Hospital ◽  
Hpv Status

AbstractObjectiveThis study associated Human Papillomavirus (HPV) infection and other clinical parameters with five-year survival of oral squamous cell carcinoma patients at a tertiary care hospital in Karachi, Pakistan.MethodsA total of 140 patients diagnosed with oral squamous cell carcinoma were enlisted. HPV status and subtypes were established through polymerase chain reaction performed in a previously published study. Clinical data including five-year survival were obtained through institutional medical records.ResultsNinety-five patients (67.9 per cent) were positive for HPV. Of these, 85 patients were HPV 16 positive while 2 patients were HPV 18 positive. The mean survival time for HPV positive patients was 44.3 months, whereas survival time for HPV negative patients was 46.9 months. Univariate analysis showed that HPV status in oral squamous cell carcinoma was not a statistically significant factor in determining five-year survival rate (p = 0.386).ConclusionThere is a high prevalence of HPV positive oral squamous cell carcinoma in Pakistan; however, there is no difference in the five-year survival rate when compared to HPV negative oral squamous cell carcinoma.

scholarly journals Genomic and epigenetic signatures associated with survival rate in oral squamous cell carcinoma patients

2018 ◽  
Vol 9 (11) ◽  
pp. 1885-1895 ◽  
Author(s):  
Ilda Patrícia Ribeiro ◽  
Francisco Caramelo ◽  
Luísa Esteves ◽  
Camila Oliveira ◽  
Francisco Marques ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Survival Rate ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Epigenetic Signatures
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