Plasma Lipidomics Identifies Unique Lipid Signatures and Potential Biomarkers for Patients With Aortic Dissection

Aortic dissection (AD) is a catastrophic cardiovascular emergency with a poor prognosis, and little preceding symptoms. Abnormal lipid metabolism is closely related to the pathogenesis of AD. However, comprehensive lipid alterations related to AD pathogenesis remain unclear. Moreover, there is an urgent need for new or better biomarkers for improved risk assessment and surveillance of AD. Therefore, an untargeted lipidomic approach based on ultra-high-performance liquid chromatograph-mass spectrometry was employed to unveil plasma lipidomic alterations and potential biomarkers for AD patients in this study. We found that 278 of 439 identified lipid species were significantly altered in AD patients (n = 35) compared to normal controls (n = 32). Notably, most lipid species, including fatty acids, acylcarnitines, cholesteryl ester, ceramides, hexosylceramides, sphingomyelins, lysophosphatidylcholines, lysophosphatidylethanolamines, phosphatidylcholines, phosphatidylinositols, diacylglycerols, and triacylglycerols with total acyl chain carbon number ≥54 and/or total double bond number ≥4 were decreased, whereas phosphatidylethanolamines and triacylglycerols with total double bond number <4 accumulated in AD patients. Besides, the length and unsaturation of acyl chains in triacylglycerols and unsaturation of 1-acyl chain in phosphatidylethanolamines were decreased in AD patients. Moreover, lysophosphatidylcholines were the lipids with the largest alterations, at the center of correlation networks of lipid alterations, and had excellent performances in identifying AD patients. The area under the curve of 1.0 and accuracy rate of 100% could be easily obtained by lysophosphatidylcholine (20:0/0:0) or its combination with lysophosphatidylcholine (17:0/0:0) or lysophosphatidylcholine (20:1/0:0). This study provides novel and comprehensive plasma lipidomic signatures of AD patients, identifies lysophosphatidylcholines as excellent potential biomarkers, and would be beneficial to the pathogenetic study, risk assessment and timely diagnosis and treatment of AD.

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Lipidomics Identified Lyso-Phosphatidylcholine and Phosphatidylethanolamine as Potential Biomarkers for Diagnosis of Laryngeal Cancer

Frontiers in Oncology ◽

10.3389/fonc.2021.646779 ◽

2021 ◽

Vol 11 ◽

Author(s):

Bo Yu ◽

Jizhe Wang

Keyword(s):

Arachidonic Acid ◽

Laryngeal Cancer ◽

High Performance ◽

High Resolution Mass Spectrometry ◽

Acyl Chain ◽

Lipid Biomarkers ◽

Molecular Characteristics ◽

Lipid Panel ◽

Lipid Species ◽

Potential Biomarkers

BackgroundLaryngeal cancer (LaC) remains one of the most common tumors of the respiratory tract with higher incidence in men than in women. The larynx is a small but vital organ on the neck. The dysfunction of the larynx can cause serious health problems such as hoarseness, respiratory distress, and dysphonia. Many lipids (e.g. phospholipid, cholesterol, fatty acid) have been recognized as a crucial role in tumorigenesis. However, the lipid biomarkers are lacking and the lipid molecular pathogenesis of LaC is still unclear.MethodsThis study aims to identify new LaC-related lipid biomarkers used for the diagnosis or early diagnosis of LaC and to uncover their molecular characteristics. Thus, we conducted serum and tissue nontargeted lipidomics study from LaC patients (n = 29) and normal controls (NC) (n = 36) via ultra-high performance liquid chromatography (UHPLC) coupled with high resolution mass spectrometry (HRMS). Multivariate and univariate statistics analyses were used to discriminate LaC patients from NC.ResultsAs expected, a lipid panel including LPC (16:0) and PE (18:0p_20:4) was defined to distinguish the LaC patients from healthy individuals with very high diagnosis performance (area under the curve (AUC) value = 1.000, sensitivity value = 1.000, and specificity value = 1.000). In addition, the levels of Cer, CerG1, SM, PC, PC-O, PE, PI, PS, and ChE in the LaC group significantly increased as compared with the NC group. However, the levels of LPC, LPC-O, LPE, LPE-p, and DG in the LaC group significantly deceased when the one was compared with the NC group. Among significantly changed lipid species, lysophospholipids containing a palmitoyl chain or an arachidonic acid acyl chain remarkably decreased and phospholipids including a palmitoyl chain or an arachidonic acid acyl chain increased in the LaC patients.ConclusionOur results not only indicate that lipidomics is powerful tool to explore abnormal lipid metabolism for the laC, but suggest that lysophospholipids and phospholipids may serve as potential biomarkers for diagnosis of LaC.

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Analysis of triacylglycerols by reversed-phase high pressure liquid chromatography with direct liquid inlet mass spectrometry

Canadian Journal of Biochemistry and Cell Biology ◽

10.1139/o83-107 ◽

1983 ◽

Vol 61 (8) ◽

pp. 840-849 ◽

Cited By ~ 54

Author(s):

L. Marai ◽

J. J. Myher ◽

A. Kuksis

Keyword(s):

High Pressure ◽

Liquid Chromatography ◽

Mass Spectrometer ◽

High Pressure Liquid Chromatography ◽

Carbon Number ◽

Bond Number ◽

Reversed Phase ◽

Phase System ◽

Liquid Chromatograph ◽

Individual Molecular Species

Natural triacylglycerols were resolved by high pressure liquid chromatography on Supelcosil LC-18 columns using a gradient of 30–90% propionitrile in acetonitrile as eluting solvent. The effluent was admitted to a quadrupole mass spectrometer via a direct liquid inlet interface. The mass spectra of the solutes were recorded in the chemical ionization mode. The triacylglycerol elution profile was obtained from the total ion current. Individual molecular species of the triacylglycerols were identified from the (MH)+ and the (MH—RCOOH)+ ions. The reversed-phase system allowed the separation of triacylglycerols on the basis of both carbon number and double-bond number, as well as of certain critical pairs of triplets of triacylglycerols with the same partition number. The method is applicable to the determination of triacylglycerol composition of both plant and animal fats. The minimum amount of sample which is required is about 50 ng in the scanning mode. However, 100 times more material must be injected in the liquid chromatograph because only 1/100th of the effluent is being injected into the mass spectrometer.

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Microstructural morphology of sphingolipid dispersions as investigated by freeze-fracture EM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100141093 ◽

1995 ◽

Vol 53 ◽

pp. 944-945

Author(s):

Vitthal S. Kulkarni ◽

Wayne H. Anderson ◽

Rhoderick E. Brown

Keyword(s):

Acyl Chain ◽

Biological Significance ◽

Freeze Fracture ◽

Fatty Acyl ◽

Fatty Acyl Moiety ◽

Aqueous Dispersions ◽

Head Groups ◽

Lipid Species ◽

Microstructural Morphology ◽

Layer Chromatography

The biological significance of the sphingomyelins (SM) and monoglycosylated sphingolipids like galactosylceramides (GalCer) are well documented Our recent investigation showed tubular bilayers in the aqueous dispersions of N-nervonoyl GalCer [N-(24:lΔ15,cls) GalCer] (a major fatty acyl moiety of natural GalCer). To determine the influence of lipid head groups on the resulting mesophasic morphology, we investigated microstructural self-assemblies of N-nervonoyl-SM [N-(24:1 Δ15,cls) SM; the second most abundant sphingomyelin in mammalian cell membranes], 1- palmitoyl-2-nervonoyl phosphatidylcholine [PNPC] (the lipid species with the same acyl chain configuration as in N-(24: 1) GalCer) and also compared it with egg-SM by freeze-fracture EM.Procedures for synthesizing and purifying N-(24:1) GalCer, N-(24:1) SM, and PNPC have been reported . Egg-SM was purchased from Avanti Polar Lipids, Alabaster AL. All lipids were >99% pure as checked by thin layer chromatography. Lipid dispersions were prepared by hydrating dry lipid with phosphate buffer (pH 6.6) at 80-90°C (3-5 min), vigorously vortexing (1 min) and repeating this procedure for three times prior to three freeze-thaw cycles.

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The Dtermination of Paracetamol by HPLC Validation of the Method and Application on Serum Samples

Revista de Chimie ◽

10.37358/rc.18.3.6163 ◽

2018 ◽

Vol 69 (3) ◽

pp. 627-631 ◽

Cited By ~ 2

Author(s):

Viorica Ohriac (Popa) ◽

Diana Cimpoesu ◽

Adrian Florin Spac ◽

Paul Nedelea ◽

Voichita Lazureanu ◽

...

Keyword(s):

Mobile Phase ◽

High Performance ◽

Hplc Analysis ◽

Emotional Experience ◽

Optimum Conditions ◽

Serum Samples ◽

Liquid Chromatograph ◽

Mobile Phase Flow Rate ◽

Quantification Limit

Pain is defined as a disagreeable sensory and emotional experience related to a tissue or potential lesion. Paracetamol (Acetaminophen) is the most used non-morphine analgesic. For the determination of paracetamol we developed and validated the high performance liquid chromatography (HPLC) analysis using a Dionex Ultimate 3000 liquid chromatograph equipped with a multidimensional detector. After determining the optimum conditions of analysis (80/20 water / acetonitrile mobile phase, flow rate 1.0 mL / min, detection wavelength 245 nm) we validated the method following the following parameters: linearity of response function, linearity of results, limit (LD = 0.66 mg / mL) and quantification limit (LQ = 2.00 mg / mL), and precision. The method of determining paracetamol by HPLC was applied to 30 samples of serum collected from patients who had pain and were treated with paracetamol.

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Effects of Natural Antioxidants on Phospholipid and Ceramide Profiles of 3D-Cultured Skin Fibroblasts Exposed to UVA or UVB Radiation

Antioxidants ◽

10.3390/antiox10040578 ◽

2021 ◽

Vol 10 (4) ◽

pp. 578

Author(s):

Agnieszka Gęgotek ◽

Wojciech Łuczaj ◽

Elżbieta Skrzydlewska

Keyword(s):

Ascorbic Acid ◽

High Performance ◽

Three Dimensional ◽

Natural Antioxidants ◽

Phospholipid Metabolism ◽

Culture Conditions ◽

Membrane Properties ◽

Dimensional System ◽

Uvb Radiation ◽

Liquid Chromatograph

Ultraviolet (UV) radiation is one of the primary factors responsible for disturbances in human skin cells phospholipid metabolism. Natural compounds that are commonly used to protect skin, due to their lipophilic or hydrophilic nature, show only a narrow range of cytoprotective activity, which prompts research on their combined application. Therefore, the aim of this study was to examine the effect of ascorbic acid and rutin on the phospholipid and ceramide profiles in UV-irradiated fibroblasts cultured in a three-dimensional system that approximates the culture conditions to the dermis. An ultra-high-performance liquid chromatograph coupled with a quadrupole time-of-flight mass spectrometer was used for phospholipid and ceramide profiling. As a result of UVA and UVB cells irradiation, upregulation of phosphatidylcholines, ceramides, and downregulation of sphingomyelins were observed, while treatment with ascorbic acid and rutin of UVA/UVB-irradiated fibroblast promoted these changes to provide cells a stronger response to stress. Moreover, an upregulation of phosphatidylserines in cells exposed to UVB and treated with both antioxidants suggests the stimulation of UV-damaged cells apoptosis. Our findings provide new insight into action of rutin and ascorbic acid on regulation of phospholipid metabolism, which improves dermis fibroblast membrane properties.

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Rapid determination of heterocyclic amines in ruminant meats using accelerated solvent extraction and ultra-high performance liquid chromatograph–mass spectrometry

MethodsX ◽

10.1016/j.mex.2019.11.014 ◽

2019 ◽

Vol 6 ◽

pp. 2686-2697

Author(s):

Charles F. Manful ◽

Natalia P. Vidal ◽

Thu H. Pham ◽

Muhammad Nadeem ◽

Evan Wheeler ◽

...

Keyword(s):

Mass Spectrometry ◽

Solvent Extraction ◽

High Performance ◽

Rapid Determination ◽

Accelerated Solvent Extraction ◽

Heterocyclic Amines ◽

High Performance Liquid Chromatograph ◽

Liquid Chromatograph

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Efficient high performance liquid chromatograph/ultraviolet method for determination of diclofenac and 4′-hydroxydiclofenac in rat serum

Journal of Chromatography B ◽

10.1016/j.jchromb.2005.10.045 ◽

2006 ◽

Vol 830 (2) ◽

pp. 231-237 ◽

Cited By ~ 26

Author(s):

Lata Kaphalia ◽

Bhupendra S. Kaphalia ◽

Santosh Kumar ◽

Mary F. Kanz ◽

Mary Treinen-Moslen

Keyword(s):

High Performance ◽

High Performance Liquid Chromatograph ◽

Rat Serum ◽

Liquid Chromatograph

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Discovery of potential biomarkers in acute kidney injury by ultra-high-performance liquid chromatography-tandem quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF–MS)

International Urology and Nephrology ◽

10.1007/s11255-021-02829-3 ◽

2021 ◽

Author(s):

Chaoyi Chen ◽

Peng Zhang ◽

Guanhu Bao ◽

Yuan Fang ◽

Wei Chen

Keyword(s):

Mass Spectrometry ◽

Acute Kidney Injury ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

Kidney Injury ◽

Flight Mass Spectrometry ◽

Tandem Quadrupole ◽

Potential Biomarkers ◽

Tof Ms

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Effect of P450 Oxidoreductase Polymorphisms on the Metabolic Activities of Ten Cytochrome P450s Varied by Polymorphic CYP Genotypes in Human Liver Microsomes

Cellular Physiology and Biochemistry ◽

10.1159/000490934 ◽

2018 ◽

Vol 47 (4) ◽

pp. 1604-1616 ◽

Cited By ~ 8

Author(s):

Yan Fang ◽

Na Gao ◽

Xin Tian ◽

Jun Zhou ◽

Hai-Feng Zhang ◽

...

Keyword(s):

Gene Polymorphisms ◽

High Performance ◽

Liver Microsomes ◽

Nucleotide Polymorphisms ◽

Liquid Chromatograph ◽

Single Nucleotide ◽

P450 Oxidoreductase ◽

Human Livers ◽

Metabolic Activities ◽

The Impact

Background/ Aims: Little is known about the effect of P450 oxidoreductase (POR) gene polymorphisms on the activities of CYPs with multiple genotypes. Methods: We genotyped 102 human livers for 18 known POR single nucleotide polymorphisms (SNPs) with allelic frequencies greater than 1% as well as for 27 known SNPs in 10 CYPs. CYP enzyme activities in microsomes prepared from these livers were determined by measuring probe substrate metabolism by high performance liquid chromatograph. Results: We found that the effects of the 18 POR SNPs on 10 CYP activities were CYP genotype-dependent. The POR mutations were significantly associated with decreased overall Km for CYP2B6 and 2E1, and specific genotypes within CYP1A2, 2A6, 2B6, 2C8, 2D6 and 2E1 were identified as being affected by these POR SNPs. Notably, the effect of a specific POR mutation on the activity of a CYP genotype could not be predicted from other CYP genotypes of even the same CYP. When combining one POR SNP with other POR SNPs, a hitherto unrecognized effect of multiple-site POR gene polymorphisms (MSGP) on CYP activity was uncovered, which was not necessarily consistent with the effect of either single POR SNP. Conclusions: The effects of POR SNPs on CYP activities were not only CYP-dependent, but more importantly, CYP genotype-dependent. Moreover, the effect of a POR SNP alone and in combination with other POR SNPs (MSGP) was not always consistent, nor predictable. Understanding the impact of POR gene polymorphisms on drug metabolism necessitates knowing the complete SNP complement of POR and the genotype of the relevant CYPs.

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