scholarly journals 7SK Acts as an Anti-tumor Factor in Tongue Squamous Cell Carcinoma

2021 ◽  
Vol 12 ◽  
Author(s):  
Bowen Zhang ◽  
Sainan Min ◽  
Qi Guo ◽  
Yan Huang ◽  
Yuzhu Guo ◽  
...  
Keyword(s):  
Squamous Carcinoma ◽  
Regulatory Sequences ◽  
Rna Seq ◽  
Motif Analysis ◽  
Migration Assay ◽  
Human Tongue ◽  
Non Coding Rna ◽  
Two Factors ◽  
Multiple Cell ◽  
Tongue Squamous Carcinoma

Increasing evidence has shown the mechanistic insights about non-coding RNA 7SK in controlling the transcription. However, the biological function and mechanism of 7SK in cancer are largely unclear. Here, we show that 7SK is down-regulated in human tongue squamous carcinoma (TSCC) and acts as a TSCC suppressor through multiple cell-based assays including a migration assay and a xenograft mouse model. The expression level of 7SK was negatively correlated with the size of tumors in the 73 in-house collected TSCC patients. Through combined analysis of 7SK knockdown of RNA-Seq and available published 7SK ChIRP-seq data, we identified 27 of 7SK-regulated genes that were involved in tumor regulation and whose upstream regulatory regions were bound by 7SK. Motif analysis showed that the regulatory sequences of these genes were enriched for transcription factors FOXJ3 and THRA, suggesting a potential involvement of FOXJ3 and THRA in 7SK-regulated genes. Interestingly, the augmented level of FOXJ3 in TSCC patients and previous reports on THRA in other cancers have suggested that these two factors may promote TSCC progression. In support of this idea, we found that 21 out of 27 aforementioned 7SK-associated genes were regulated by FOXJ3 and THRA, and 12 of them were oppositely regulated by 7SK and FOXJ3/THRA. We also found that FOXJ3 and THRA dramatically promoted migration in SCC15 cells. Collectively, we identified 7SK as an antitumor factor and suggested a potential involvement of FOXJ3 and THRA in 7SK-mediated TSCC progression.

scholarly journals Electrochemical Assessment of Anticancer Compounds on the Human Tongue Squamous Carcinoma Cells

Sensors ◽  
2020 ◽  
Vol 20 (9) ◽  
pp. 2632 ◽  
Author(s):  
Chun-Chung Huang ◽  
Tse-Hua Tung ◽  
Chien-Chu Huang ◽  
Shao-Yi Lin ◽  
Shih-Chi Chao ◽  
...  
Keyword(s):  
Cell Morphology ◽  
Squamous Carcinoma ◽  
Annexin V ◽  
Time Dependent ◽  
Second Primary ◽  
Second Primary Malignancy ◽  
Primary Malignancy ◽  
Squamous Carcinoma Cells ◽  
Human Tongue ◽  
Tongue Squamous Carcinoma

The most common oral cancer is squamous cell carcinoma (SCC) and its highest occurrence is in the tongue. Almost 30% of patients with one primary head and neck tumor will have a second primary malignancy. In recent studies, two novel plant extracts, andrographolide and cannabidiol (CBD), have been exploited for their anticancer effects. Here, we investigated the cytotoxic effects of these two compounds on SCC-25 cells, a human tongue squamous carcinoma cell line, and compared the outcomes with two chemotherapeutic drugs, cisplatin and fluorouracil. Electric cell substrate impedance sensing (ECIS) system was applied to measure frequency- and time-dependent impedance of SCC-25 cell-covered electrodes and to further assess subtle changes in cell morphology and micromotion in response to different concentrations (0, 10, 30, 100, and 300 µM) of these compounds. AlamarBlue and Annexin V/7-AAD binding assays were used to measure the concentration dependent changes in viability and apoptosis of SCC-25 cells. Our results demonstrate that 24 hours after exposure to 30 µM CBD can significantly decrease the micromotion rate, damage the integrity of cell morphology, reduce cell viability, and induce higher apoptosis in treated SCC-25 cells, while the other three drugs attain similar effects at the concentration of 100 µM or higher. The apoptosis-induced changes in cell morphology and micromotion monitored by ECIS correlate well with biochemical assays. Thus, both frequency- and time-dependent impedance measurements using ECIS can be used to real-time follow cancer cell activities in response to anticancer drugs with different temporal cytotoxicity profiles.

scholarly journals Synthetic Isoliquiritigenin Inhibits Human Tongue Squamous Carcinoma Cells through Its Antioxidant Mechanism

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Cuilan Hou ◽  
Wenguang Li ◽  
Zengyou Li ◽  
Jing Gao ◽  
Zhenjie Chen ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Fluorescence Microscopy ◽  
Gelatin Zymography ◽  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Migration And Invasion ◽  
Antioxidant Mechanism ◽  
Squamous Carcinoma Cells ◽  
Human Tongue ◽  
Tongue Squamous Carcinoma

Isoliquiritigenin (ISL), a natural antioxidant, has antitumor activity in different types of cancer cells. However the antitumor effect of ISL on human tongue squamous carcinoma cells (TSCC) is not clear. Here we aimed to investigate the effects of synthetic isoliquiritigenin (S-ISL) on TSCC and elucidate the underlying mechanisms. S-ISL was synthesized and elucidated from its nuclear magnetic resonance spectrum and examined using high performance liquid chromatography. The effects of S-ISL on TSCC cells (Tca8113) were evaluated in relation to cell proliferation, apoptosis and adhesion, migration, and invasion using sulforhodamine B assay, fluorescence microscopy technique, flow cytometry (FCM) analysis, and Boyden chamber assay. The associated regulatory mechanisms were examined using FCM and fluorescence microscopy for intracellular reactive oxygen species (ROS) generation, Gelatin zymography assay for matrix metalloproteinase (MMP) activities, and Western blot for apoptosis regulatory proteins (Bcl-2 and Bax). Our data indicated that S-ISL inhibited Tca8113 cell proliferation, adhesion, migration, and invasion while promoting the cell apoptosis. Such effects were accompanied by downregulation of Bcl-2 and upregulation of Bax, reduction of MMP-2 and MMP-9 activities, and decreased ROS production. We conclude that S-ISL is a promising agent targeting TSCC through multiple anticancer effects, regulated by its antioxidant mechanism.

Berberine inhibits human tongue squamous carcinoma cancer tumor growth in a murine xenograft model

Phytomedicine ◽  
2009 ◽  
Vol 16 (9) ◽  
pp. 887-890 ◽  
Author(s):  
Yung-Tsuan Ho ◽  
Jai-Sing Yang ◽  
Chi-Cheng Lu ◽  
Jo-Hua Chiang ◽  
Tsai-Chung Li ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Xenograft Model ◽  
Squamous Carcinoma ◽  
Human Tongue ◽  
Murine Xenograft Model ◽  
Cancer Tumor ◽  
Tongue Squamous Carcinoma

scholarly journals Model of human tongue squamous cell line stably transfected with human papillomavirus（HPV) 16 E6 and E7 genes and biological characteristic analysis

2020 ◽  
Author(s):  
Zilian Lan ◽  
Ziyao Jia ◽  
Hengyuan Guo ◽  
Zhaoshou Yang ◽  
Zifan Yang ◽  
...  
Keyword(s):  
Cell Lines ◽  
Carcinoma Cell ◽  
Squamous Carcinoma ◽  
Migration Ability ◽  
Carcinoma Cell Lines ◽  
Human Tongue ◽  
Squamous Carcinoma Cell ◽  
And Migration ◽  
Tongue Squamous Carcinoma ◽  
Proliferation And Migration

Abstract Human tongue squamous carcinoma cell lines transfected with HPV16E6E7 gene were established to provide a model for further study of HPV16 E6E7-related human tongue squamous carcinoma cell lines .Plasmid pEGFR/HPV16 of E6E7 and plasmid pEGFR/HPV16 of No E6E7 were constructed.Human tongue squamous carcinoma cell lines including SCC9 and SCC15 were infected by liposome transfection and would be highly selected by antibiotic .Fluorescence imaging, RT-PCR and Westernblot were used to detect the expression of HPV16 E6E7 in cells.The biological characteristics of human tongue squamous carcinoma cell lines infected with HPV16 E6E7 were detected by CCK-8 and wound healing assay. The human tongue squamous carcinoma cell lines transfected with HPV16 E6E7 gene were successfully established and identified, and the proliferation and migration ability of the human tongue squamous carcinoma cell lines infected with HPV16 E6E7 gene was significantly stronger than that of the blank group.Human tongue squamous carcinoma cell lines infected with HPV16 E6E7 were more malignant, and their proliferation and migration ability were higher than those not infected with HPV16 E6E7.

scholarly journals Identification of Cis-Regulatory Sequences Controlling Pollen-Specific Expression of Hydroxyproline-Rich Glycoprotein Genes in Arabidopsis thaliana

2020 ◽  
Author(s):  
Yichao Li ◽  
Maxwell Mullin ◽  
Yingnan Zhang ◽  
Frank Drews ◽  
Lonnie Welch ◽  
...  
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Motif Discovery ◽  
Plant Cell Wall ◽  
Integrative Analysis ◽  
Regulatory Sequences ◽  
Rna Seq ◽  
Motif Analysis ◽  
Specific Expression ◽  
Regulatory Motifs

ABSTRACTHydroxyproline-rich glycoproteins (HRGPs) are a superfamily of plant cell wall structural proteins that function in various aspects of plant growth and development, including pollen tube growth. We have previously characterized HRGP superfamily into three family members: the hyperglycosylated arabinogalactan-proteins, the moderately glycosylated extensins, and the lightly glycosylated proline-rich proteins. However, the mechanism of pollen-specific HRGP expression remains untouched. To this end, we developed an integrative analysis pipeline combining RNA-seq gene expression and promoter sequences that identified 15 transcriptional cis-regulatory motifs responsible for pollen-specific expression of HRGP in Arabidopsis Thaliana. Specifically, we mined the public RNA-seq datasets and identified 13 pollen-specific HRGP genes. Ensemble motif discovery with various filters identified 15 conserved promoter elements between Thaliana and Lyrata. Known motif analysis revealed pollen related transcription factors of GATA12 and brassinosteroid (BR) signaling pathway regulator BZR1. Lastly, we performed a machine learning regression analysis and demonstrated that the identified 15 motifs well captured the HRGP gene expression in pollen (R=0.61). In conclusion, we performed the integrative analysis as the first-of-its-kind study to identify cis-regulatory motifs in pollen-specific HRGP genes and shed light on its transcriptional regulation in pollen.

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