scholarly journals Exploring the Role of Autophagy-Related Gene 5 (ATG5) Yields Important Insights Into Autophagy in Autoimmune/Autoinflammatory Diseases

2018 ◽  
Vol 9 ◽  
Author(s):  
Xin Ye ◽  
Xu-Jie Zhou ◽  
Hong Zhang
Keyword(s):  
Autoinflammatory Diseases ◽  
Related Gene

scholarly journals Modulation of Cathepsin S (CTSS) Regulates the Secretion of Progesterone and Estradiol, Proliferation, and Apoptosis of Ovarian Granulosa Cells in Rabbits

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1770
Author(s):  
Guohua Song ◽  
Yixuan Jiang ◽  
Yaling Wang ◽  
Mingkun Song ◽  
Xuanmin Niu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Granulosa Cells ◽  
Hormone Secretion ◽  
Vital Role ◽  
Regulatory Function ◽  
Cathepsin S ◽  
Related Gene ◽  
Ovarian Granulosa Cells ◽  
Proliferation And Apoptosis

Cathepsin S (CTSS) is a member of cysteine protease family. Although many studies have demonstrated the vital role of CTSS in many physiological and pathological processes including tumor growth, angiogenesis and metastasis, the function of CTSS in the development of rabbit granulosa cells (GCS) remains unknown. To address this question, we isolated rabbit GCS and explored the regulatory function of the CTSS gene in cell proliferation and apoptosis. CTSS overexpression significantly promoted the secretion of progesterone (P4) and estrogen (E2) by increasing the expression of STAR and CYP19A1 (p < 0.05). We also found that overexpression of CTSS increased GCS proliferation by up-regulating the expression of proliferation related gene (PCNA) and anti-apoptotic gene (BCL2). Cell apoptosis was markedly decreased by CTSS activation (p < 0.05). In contrast, CTSS knockdown significantly decreased the secretion of P4 and E2 and the proliferation of rabbit GCS, while increasing the apoptosis of rabbit GCS. Taken together, our results highlight the important role of CTSS in regulating hormone secretion, cell proliferation, and apoptosis in rabbit GCS. These results might provide a basis for better understanding the molecular mechanism of rabbit reproduction.

Regulation of Pax-3 expression in the dermomyotome and its role in muscle development

Development ◽  
1994 ◽  
Vol 120 (4) ◽  
pp. 957-971 ◽  
Author(s):  
M. Goulding ◽  
A. Lumsden ◽  
A.J. Paquette
Keyword(s):  
Transcription Factors ◽  
Muscle Development ◽  
Cell Types ◽  
Axial Skeleton ◽  
Mouse Embryos ◽  
Related Gene ◽  
Paired Domain ◽  
Trunk Musculature ◽  
Somitic Mesoderm

The segmented mesoderm in vertebrates gives rise to a variety of cell types in the embryo including the axial skeleton and muscle. A number of transcription factors containing a paired domain (Pax proteins) are expressed in the segmented mesoderm during embryogenesis. These include Pax-3 and a closely related gene, Pax-7, both of which are expressed in the segmental plate and in the dermomyotome. In this paper, we show that signals from the notochord pattern the expression of Pax-3, Pax-7 and Pax-9 in somites and the subsequent differentiation of cell types that arise from the somitic mesoderm. We directly assess the role of the Pax-3 gene in the differentiation of cell types derived from the dermomyotome by analyzing the development of muscle in splotch mouse embryos which lack a functional Pax-3 gene. A population of Pax-3-expressing cells derived from the dermomyotome that normally migrate into the limb are absent in homozygous splotch embryos and, as a result, limb muscles are lost. No abnormalities were detected in the trunk musculature of splotch embryos indicating that Pax-3 is necessary for the development of the limb but not trunk muscle.

scholarly journals Role of mica repeat polymorphism in the manifestation of type 1 diabetes mellitus in Bengali Indian patients

Genetika ◽  
2013 ◽  
Vol 45 (2) ◽  
pp. 611-619
Author(s):  
Oindrila Raha ◽  
B Sarkar ◽  
P. Veerraju ◽  
G. Sudhakar ◽  
P. Raychaudhuri ◽  
...  
Keyword(s):  
Indian Population ◽  
Healthy Controls ◽  
Related Gene ◽  
Repeat Polymorphism ◽  
Histocompatibility Complex ◽  
Indian Patients ◽  
Mica Gene ◽  
Polymorphic Gene

Background. The major histocompatibility complex class I chain-related gene A (MICA) (OMIM-600169) is a polymorphic gene in the HLA region expressed mainly by epithelial cells. The MICA protein encoded by the allele influences the activation of NK cells, which modify ?-cells destruction and has been found to be involved in susceptibility of T1DM. Objective. The aim of this study was to find the association of MICA alleles with T1DM among eastern Indian population. Subjects and methods.Study was conducted in 134 eastern Indian patients and with 137 healthy controls for the possible role of MICA gene in T1DM pathogenesis. Results. The MICA*A5 microsatellite allele, showed significantly higher frequencies in patients than controls (p=0.003, OR= 1.746, CI= 1.206-2.528). MICA A*6 was found to be protective in our study (p=<0.01, OR=0.406, CI= 0.268-0.616).

scholarly journals The critical role of ASD-related gene CNTNAP3 in regulating synaptic development and social behavior in mice

2019 ◽  
Vol 130 ◽  
pp. 104486 ◽  
Author(s):  
Da-li Tong ◽  
Rui-guo Chen ◽  
Yu-lan Lu ◽  
Wei-ke Li ◽  
Yue-fang Zhang ◽  
...  
Keyword(s):  
Social Behavior ◽  
Critical Role ◽  
Related Gene ◽  
Synaptic Development

scholarly journals The Role of Cutaneous Type I IFNs in Autoimmune and Autoinflammatory Diseases

2020 ◽  
Vol 205 (11) ◽  
pp. 2941-2950
Author(s):  
Jessica L. Turnier ◽  
J. Michelle Kahlenberg
Keyword(s):  
Autoinflammatory Diseases ◽  
Type I ◽  
Type I Ifns

scholarly journals The role of ERG (ets related gene) in cartilage development

2001 ◽  
Vol 9 ◽  
pp. S41-S47 ◽  
Author(s):  
M. Iwamoto ◽  
Y. Higuchi ◽  
M. Enomoto-Iwamoto ◽  
K. Kurisu ◽  
E. Koyama ◽  
...  
Keyword(s):  
Related Gene ◽  
Cartilage Development

Structural Requirements of Human Ether-a-go-go–  related Gene Channels for Block by Bupivacaine

2007 ◽  
Vol 106 (3) ◽  
pp. 523-531 ◽  
Author(s):  
Cornelia C. Siebrands ◽  
Patrick Friederich
Keyword(s):  
Aromatic Amino Acids ◽  
Selectivity Filter ◽  
Site Directed Mutagenesis ◽  
Xenopus Laevis Oocytes ◽  
Related Gene ◽  
Wild Type ◽  
Aromatic Residues ◽  
Ic50 Value ◽  
Herg Channels

Background Local anesthetics interact with human ether-a-go-go-related gene (HERG) channels via the aromatic amino acids Y652 and F656 in the S6 region. This study aimed to establish whether the residues T623, S624, and V625 residing deeper within the pore are also involved in HERG channel block by bupivacaine. In addition, the study aimed to further define the role of the aromatic residues Y652 and F656 in bupivacaine inhibition by mutating these residues to threonine. Methods Alanine and threonine mutants were generated by site-directed mutagenesis. Electrophysiologic and pharmacologic properties of wild-type and mutant HERG channels were established using two-electrode voltage-clamp recordings of Xenopus laevis oocytes expressing HERG channels. Results Tail currents at -120 mV through HERG wild-type channels were inhibited with an IC50 value of 132 +/- 22 microm (n = 33). Bupivacaine (300 microm) inhibited wild-type tail currents by 62 +/- 12% (n = 7). Inhibition of HERG tail currents by bupivacaine (300 microm) was reduced by all mutations (P &lt; 0.001). The effect was largest for F656A (inhibition 5 +/- 2%, n = 6) in the lower S6 region and for T623A (inhibition 13 +/- 4%, n = 9) near the selectivity filter. Introducing threonine at positions 656 and 652 significantly reduced inhibition by bupivacaine compared with HERG wild type (P &lt; 0.001). Conclusions The authors' results indicate that not only the aromatic residues Y652 and F656 but also residues residing deeper within the pore and close to the selectivity filter of HERG channels are involved in inhibition of HERG channels by the low-affinity blocker bupivacaine.

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