scholarly journals Multiple Modes of Nematode Control by Volatiles of Pseudomonas putida 1A00316 from Antarctic Soil against Meloidogyne incognita

2018 ◽  
Vol 9 ◽  
Author(s):  
Yile Zhai ◽  
Zongze Shao ◽  
Minmin Cai ◽  
Longyu Zheng ◽  
Guangyu Li ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Meloidogyne Incognita ◽  
Antarctic Soil ◽  
Nematode Control ◽  
Multiple Modes

scholarly journals Cyclo(l-Pro–l-Leu) of Pseudomonas putida MCCC 1A00316 Isolated from Antarctic Soil: Identification and Characterization of Activity against Meloidogyne incognita

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 768 ◽  
Author(s):  
Yile Zhai ◽  
Zongze Shao ◽  
Minmin Cai ◽  
Longyu Zheng ◽  
Guangyu Li ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Pseudomonas Putida ◽  
Meloidogyne Incognita ◽  
High Performance ◽  
Larval Mortality ◽  
Control Treatment ◽  
Hatching Rate ◽  
Antarctic Soil ◽  
Egg Hatching ◽  
Flight Mass Spectrometry

Pseudomonas putida MCCC 1A00316 was originally isolated from an Antarctic soil and has demonstrated potential nematicidal activity. Thus, it has promising applications for the biological control of Meloidogyne incognita. The larval mortality and egg-hatching inhibition rates of M. incognita will increase with the rising concentration of culture filtrates of P. putida MCCC 1A00316 and the duration of exposure. Thus, this study aimed to separate, purify, and identify nematicidal compounds from P. putida MCCC 1A00316 and to validate their anti-M. incognita activities. Compounds were purified through silica gel column chromatography and thin-layer chromatography combined with high-performance liquid chromatography (HPLC). Structural identification was conducted through liquid chromatography time-of-flight mass spectrometry, 1H nuclear magnetic resonance (NMR) spectroscopy, 13C-NMR, and Marfey’s method. The isolated compounds were identified as cyclo(l-Pro–l-Leu) on the basis of the results of the above analyses and previously reported data. The effects of various concentrations of cyclo(l-Pro–l-Leu) on the mortality rates of second-stage juveniles (J2) of M. incognita were investigated. Results showed that HPLC-purified cyclo(l-Pro–l-Leu) displayed nematicidal activities. The mortality rate of M. incognita J2 reached 84.3% after 72 h of exposure to 67.5 mg/L cyclo(l-Pro–l-Leu). The lowest egg-hatching rate (9.74%) was observed after 8 days of incubation with 2000 mg/L cyclo(l-Pro–l-Leu). An egg-hatching rate of 53.11% was obtained under the control treatment (sterile distilled water). However, cyclo(l-Pro–l-Leu) did not elicit chemotaxis activity to M. incognita. This is the first work to investigate the anti-M. incognita characteristics of cyclo(l-Pro–l-Leu).

scholarly journals Potential of Pseudomonas putida, Bacillus subtilis, and their mixture on the management of Meloidogyne incognita, Pectobacterium betavasculorum, and Rhizoctonia solani disease complex of beetroot (Beta vulgaris L.)

2019 ◽  
Vol 29 (1) ◽  
Author(s):  
Manzoor R. Khan ◽  
Zaki A. Siddiqui
Keyword(s):  
Bacillus Subtilis ◽  
Rhizoctonia Solani ◽  
Pseudomonas Putida ◽  
Beta Vulgaris ◽  
Meloidogyne Incognita ◽  
Root Rot ◽  
Growth Parameters ◽  
Principal Component ◽  
Soft Rot ◽  
Disease Complex

AbstractEffects of Pseudomonas putida and Bacillus subtilis alone, and in combinations for the management of Meloidogyne incognita, Pectobacterium betavasculorum, and Rhizoctonia solani disease complex of beetroot (Beta vulgaris L.), were studied. Application of P. putida or B. subtilis to plants with M. incognita or P. betavasculorum or R. solani singly or in combinations caused a significant increase in plant growth parameters and the activities of defense enzymes. A significant increase in chlorophyll fluorescence attributes, viz., Fv/Fm, ɸPSII, qP, NPQ, and ETR were recorded in plants treated with P. putida or B. subtilis over pathogen-inoculated plants. Inoculation of P. putida results in a higher reduction in galling and nematode multiplication than B. subtilis. Maximum reduction in nematode multiplication and galling occurred when a mixture of P. putida and B. subtilis was used. Soft rot and root rot indices were 3 when Pectobacterium betavasculorum and Rhizoctonia solani were inoculated alone. The disease indices were rated 5 when these pathogens and M. incognita were inoculated in combinations. Inoculation of P. putida/B. subtilis with P. betavasculorum or R. solani reduced soft rot and root rot indices to 2 out of 3, while the use of P. putida + B. subtilis reduced indices to 1. Disease indices were reduced to 2–3 out of 5, when P. putida + B. subtilis were used to plants inoculated with two or three pathogens. The principal component analysis showed significant correlations among the various studied attributes. Two principal components explained a total of 86.1 and 93.4% of the overall data variability. Therefore, the use of P. putida together with B. subtilis had the potential for successful management of disease complex of beetroot.

scholarly journals Insect and Nematode Control in Cucumber (Cucumis sativus) in Puerto Rico.

1969 ◽  
Vol 70 (1) ◽  
pp. 19-24
Author(s):  
Nelia Acosta ◽  
Carlos Cruz ◽  
José Negrón
Keyword(s):  
Puerto Rico ◽  
Meloidogyne Incognita ◽  
Chemical Control ◽  
Field Experiments ◽  
Lower Number ◽  
Nematode Control ◽  
Rotylenchulus Reniformis ◽  
Liriomyza Sativae ◽  
The Third ◽  
Cucumber Cultivar

Field experiments for the chemical control of the pickleworm (Diaphania nitidalis), the melonworm (D. hyalinata), the leafminer (Liriomyza sativae), and nematodes (Meloidogyne incognita and Rotylenchulus reniformis) on cucumber cultivar Ashley were established in two locations in Puerto Rico in 1979 and 1980. In the second test, a lower number of mined leaves was observed on methamidophos-treated plots as well as a significant lower index of leafminer damage. All chemicals controlled the pickleworm effectively. Highest yields were obtained from plots treated with the highest dosage of acephate and with the intermediate dose of methamidophos. Applications of carbofuran, acephate, methomyl and permethrin reduced the melonworm damage in the third test. Highest yields were obtained from carbofuran-treated plots. All nematicides controlled nematodes in the soil.

Tomato culture filtrate stimulates hatching and activity of Meloidogyne incognita juveniles

Nematology ◽  
2009 ◽  
Vol 11 (1) ◽  
pp. 51-61 ◽  
Author(s):  
Takayuki Mizukubo ◽  
Yuji Oka
Keyword(s):  
Meloidogyne Incognita ◽  
Laboratory Experiments ◽  
Culture Medium ◽  
Culture Media ◽  
Nematode Control ◽  
Second Stage ◽  
Plant Culture ◽  
Fresh Culture Medium ◽  
Fresh Culture ◽  
Tomato Culture

AbstractHydroponic culture media were tested after growing tomato, okra, cucumber and bean for their effect on hatching and mobility of Meloidogyne incognita second-stage juveniles (J2) in laboratory experiments. Tomato and okra culture media increased the numbers of J2 that hatched as compared to those in water or fresh culture medium. The tomato culture medium increased hatching even in the presence of fosthiazate, an organophosphate nematicide, and at a concentration that inhibited hatching in the absence of tomato culture medium. Neither heat treatment of the tomato culture medium nor change of pH abolished its hatching stimulatory activity. When active J2 were incubated in the tomato culture media, the percentages of nematodes that became quiescent were lower than those of nematodes incubated in water or in fresh culture medium for 3 and 7 days in two trials. Moreover, the sigmoid movement of J2 was faster in the tomato culture medium than in water. Quiescent J2 became active more rapidly in culture media of tomato, cucumber and okra than in water or fresh culture medium. In experiments using soil containing quiescent J2, more J2 were extracted with the Baermann funnel method from soil treated with tomato culture medium than from soil treated with water. The results confirm that nematode stimulants, which may serve as a potential means in nematode control, exist in the plant culture media.

Screening plants resistant against Meloidogyne incognita and integrated management of plant resources for nematode control

2012 ◽  
Vol 33 ◽  
pp. 34-39 ◽  
Author(s):  
Linlin Dong ◽  
Chengdong Huang ◽  
Li Huang ◽  
Xiaolin Li ◽  
Yuanmei Zuo
Keyword(s):  
Meloidogyne Incognita ◽  
Integrated Management ◽  
Nematode Control ◽  
Plant Resources

Resistance mechanisms ofPrunusrootstocks to root-knot nematode,Meloidogyne incognita

Fruits ◽  
2009 ◽  
Vol 64 (5) ◽  
pp. 295-303 ◽  
Author(s):  
Hang Ye ◽  
Wen-jun Wang ◽  
Guo-jie Liu ◽  
Li-xin Zhu ◽  
Ke-gong Jia
Keyword(s):  
Meloidogyne Incognita ◽  
Resistance Mechanisms ◽  
Root Knot Nematode

Effect of Homeopathic Preparations on Lettuce, Parasitized or Not by Meloidogyne enterolobii

Homeopathy ◽  
2020 ◽  
Author(s):  
Thais Moraes Ferreira ◽  
Mariana Zandomênico Mangeiro ◽  
Alexandre Macedo Almeida ◽  
Ricardo Moreira Souza
Keyword(s):  
Plant Pathogens ◽  
Nematode Control ◽  
Plant Tolerance ◽  
Nematode Reproduction ◽  
Meloidogyne Enterolobii ◽  
Negative Effect ◽  
Meloidogyne Spp ◽  
Constant Dose ◽  
Lettuce Growth ◽  
Complementary Strategy

Abstract Background There are relatively few scientific works on the use of homeopathy to manage plant pathogens, particularly nematodes. A handful of studies focused on Meloidogyne spp. parasitizing vegetables have brought contradictory results on nematode control and enhancement of plant tolerance to parasitism. Objective Our goal was to assess the effect of Cina—a well-known anti-nematode ingredient—on Meloidogyne enterolobii parasitizing lettuce. Methods Cina was applied daily on nematode-inoculated plants, from the seedling stage until harvest. We tested an evenly spaced range of Hahnemannian concentrations (c), which were applied though irrigation with a constant dose of the ingredient. Several absolute and relative controls were employed to allow the assessment of the effect of Cina on nematode reproduction and lettuce growth. Results Cina affected growth of non-parasitized plants, both positively and negatively; this effect was modulated by the c applied and the thermal stress suffered by the plants in one of the assays. The effect of Cina on the growth of nematode-parasitized plants was neutral or negative. Cina reduced nematode reproduction by 25–36%. Conclusion Based on the moderate negative effect of Cina on M. enterolobii reproduction, it seems this ingredient may be useful as a complementary strategy for Meloidogyne control. But Cina did not enhance the tolerance of lettuce to Meloidogyne spp.

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