Hydrogel Containing Oleoresin From Copaifera officinalis Presents Antibacterial Activity Against Streptococcus agalactiae

Background: The present investigation of a mangrove plant, Excoecaria agallocha, which is a popular medicinal substitute for the treatment of microbial ailments, were evaluated for potential antimicrobial activity against pathogenic bacteria Escherichia coli and Streptococcus agalactiae in tilapia, Oreochromis niloticus. Methods: Antibacterial activity was performed using agar diffusion method, disc diffusion method, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and antibiotic susceptibility assays. Experimental fish fed diet containing 0 (control), 5, 25, and 50 mg kg-1 E. agallocha leaf methanol extract for 28 days then challenged individually with E. coli or S. agalactiae and mortalities were recorded over a ten-day post-infection period. Results: Results indicated that both bacterial species are sensitive to tetracycline, ampicillin, and amoxicillin. E. coli was found to be resistant to neomycin. E. agallocha extract concentration of 50 mg/ml produced a zone of inhibition of 18 mm against E. coli, in contrast to 13 mm against S. agalactiae. E. agallocha showed bactericidal activity against E. coli and bacteriostatic activity against S. agalactiae. The highest E. agallocha LC50 activity was 83 mg/ml. The highest cumulative mortality was 90.0 ± 10.0% in control as compared to 26.7 ± 11.5% in the group fed with 50 mg kg-1 E. agallocha extract, significant differences (P < 0.05). Conclusion: Hence, E. agallocha showed antibacterial activity against fish pathogens Escherichia coli and Streptococcus agalactiae in tilapia, Oreochromis niloticus; therefore, E. agallocha may be used as an alternative therapeutic agent against fish pathogenic bacteria as an additive to feed at a concentration depend, safe, non-cytotoxic doses.

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POTENSI ANTIBAKTERI BINTANG LAUT DARI PERAIRAN PANTAI KELURAHAN TONGKAINA MANADO

JURNAL PESISIR DAN LAUT TROPIS ◽

10.35800/jplt.7.3.2019.24431 ◽

2019 ◽

Vol 7 (3) ◽

pp. 167

Author(s):

Delpris Piter ◽

Esther D Angkouw ◽

Fitje Losung

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Streptococcus Agalactiae ◽

Antibacterial Agents ◽

Sea Water ◽

Diffusion Method ◽

E Coli ◽

Linckia Laevigata ◽

And Staphylococcus Aureus

Starfish is one of the species of the asteroidean class, and is grouped into phylum echinoderms. Echinoderms consist of approximately 6000 species and all live in sea water. Starfish is one source bioactive compounds. Active compounds from starfish have been known to have activities antioxidant, antibacterial, anti-inflammatory, antifungal and immunostimulatory, there are also blue starfish that are potential as antitumor and antibacterial agents. This study aims to obtain crude extracts from several types of starfish, and testing the antibacterial activity of crude starfish extracts against bacteria Streptococcus agalactiae, Escherichia coli and Staphylococcus aureus. Test the antibacterial activity of crude starfish extracs Protoreaster nodosus, Achaster tipycus, dan Linckia laevigata against bacteria E. coli, S. aureus, dan S. agalactiae use the agar diffusion method by means of a well. L. l extract can inhibit bacteria e. coli at an extract concentration of 1000 mg/ml with a diameter of inhibitory zone 11.0 mm, whereas at a concentration of 750 mg/ml the test bacteria can still grow on the bacteria S. agalactiae and S. aureus can still grow even though the extract has be given L. l Keywords: Potential Starfish, Antibacterial Activity, Staphylococcus aureus, Escherichia coli, and Streptococcus agalactiae Bintang laut merupakan salah satu spesies dari kelas Asteroidea, dan dikelompokkan kedalam filum Echinodermata. Filum Echinodermata terdiri atas kurang lebih 6000 spesies dan semuanya hidup di air laut. Bintang laut merupakan salah satu sumber penghasil senyawa bioaktif. Senyawa aktif dari bintang laut telah diketahui memiliki aktivitas antioksidan, antibakteri, antiinflamasi, antifungi dan imunostimulator, ada juga bintang laut biru yang potensial sebagai antitumor dan agen antibakteri. Penelitian ini bertujuan untuk mendapatkan ekstrak kasar dari beberapa jenis bintang laut, dan menguji aktivitas antibakteri ekstrak kasar bintang laut terhadap bakteri Streptococcus agalactiae, Escherichia coli dan Staphylococcus aureus. Uji aktivitas antibakteri dari ekstrak kasar bintang laut Protoreaster nodosus, Achaster tipycus, dan Linckia laevigata terhadap bakteri E. coli, S. aureus, dan S. agalactiae mengggunakan metode difusi agar dengan cara sumur. Ekstrak L. l mampu menghambat bakteri E.coli pada konsentrasi ekstrak 1.000 mg/ml dengan diameter zona hambat 11.0 mm, sedangkan pada konsentrasi 750 mg/ml bakteri uji masih bisa bertumbuh pada bakteri S. agalactiae dan S. aureus masih dapat bertumbuh walaupun telah diberikan ekstrak L. l.Kata Kunci : Potensi Bintang Laut, Aktivitas Antibakteri, Streptococcus agalactiae, Escherichia coli and Staphylococcus aureus

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Antibacterial activity of Anadendrum microstachyum and Selaginella plana against two pathogenic bacteria (Edwardsiella ictaluri and Streptococcus agalactiae) causing freshwater fish diseases

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/535/1/012025 ◽

2020 ◽

Vol 535 ◽

pp. 012025

Author(s):

H Novita ◽

A D Novitaningrum ◽

B D Madusari ◽

M F I Nugraha ◽

M A Rajamuddin ◽

...

Keyword(s):

Antibacterial Activity ◽

Freshwater Fish ◽

Streptococcus Agalactiae ◽

Pathogenic Bacteria ◽

Edwardsiella Ictaluri ◽

Fish Diseases

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Evaluation of the antibacterial activity of tilmicosin-SLN against Streptococcus agalactiae: in vitro and in vivo studies

International Journal of Nanomedicine ◽

10.2147/ijn.s168179 ◽

2018 ◽

Vol Volume 13 ◽

pp. 4747-4755 ◽

Cited By ~ 7

Author(s):

Luyan Zhu ◽

Xiaoxia Cao ◽

Qinxin Xu ◽

Jing Su ◽

Xihe Li ◽

...

Keyword(s):

Antibacterial Activity ◽

Streptococcus Agalactiae ◽

In Vivo Studies

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Secondary metabolites of Antarctic fungi antagonistic to aquatic pathogenic bacteria

Open Life Sciences ◽

10.1515/biol-2018-0002 ◽

2018 ◽

Vol 13 (1) ◽

pp. 11-21 ◽

Cited By ~ 1

Author(s):

Huibin Zhao ◽

Chuner Cai ◽

Xiaoyu Liu ◽

Binghua Jiao ◽

Bo Chen ◽

...

Keyword(s):

Antibacterial Activity ◽

Secondary Metabolites ◽

Molecular Identification ◽

Streptococcus Agalactiae ◽

Aeromonas Hydrophila ◽

Pathogenic Bacteria ◽

Vibrio Anguillarum ◽

Fermentation Liquor ◽

Ph Curve ◽

First Time

AbstractPolar microbial derived antibiotics have potential as alternatives to traditional antibiotics in treating fish against pathogenic bacteria. In this paper, 23 strains of polar fungi were fermented to detect bacteriostatic products on three aquatic pathogenic bacteria, subsequently the active fungus was identified. It was indicated that secondary metabolites of 23 strains weredistinct; of these, the extract of strain B-7 (belonging toBjerkanderaaccording to molecular identification) demonstrated a strong antibacterial activity toStreptococcus agalactiae,Vibrio anguillarumandAeromonas hydrophilaATCC7966 by Kirby-Bauerpaper strip method. During one fermentation cycle, the pH curve of the fermentation liquor became lowest (4.0) on the 4thday and rose back to 7.6 finally after 5 days, The residual sugar curve was decreased before stablising on the 6thday. It is presumed that a large amount of alkaline secondary metabolites might have been produced during fermentation. This study focuses on antagonism between aquatic pathogenic bacteria and fermentation metabolites from Antarctic fungi for the first time, which may provide data on research of antibiotics against aquatic pathogenic bacteria.

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