scholarly journals Secondary metabolites of Antarctic fungi antagonistic to aquatic pathogenic bacteria

2018 ◽  
Vol 13 (1) ◽  
pp. 11-21 ◽  
Author(s):  
Huibin Zhao ◽  
Chuner Cai ◽  
Xiaoyu Liu ◽  
Binghua Jiao ◽  
Bo Chen ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Molecular Identification ◽  
Streptococcus Agalactiae ◽  
Aeromonas Hydrophila ◽  
Pathogenic Bacteria ◽  
Vibrio Anguillarum ◽  
Fermentation Liquor ◽  
Ph Curve ◽  
First Time

AbstractPolar microbial derived antibiotics have potential as alternatives to traditional antibiotics in treating fish against pathogenic bacteria. In this paper, 23 strains of polar fungi were fermented to detect bacteriostatic products on three aquatic pathogenic bacteria, subsequently the active fungus was identified. It was indicated that secondary metabolites of 23 strains weredistinct; of these, the extract of strain B-7 (belonging toBjerkanderaaccording to molecular identification) demonstrated a strong antibacterial activity toStreptococcus agalactiae,Vibrio anguillarumandAeromonas hydrophilaATCC7966 by Kirby-Bauerpaper strip method. During one fermentation cycle, the pH curve of the fermentation liquor became lowest (4.0) on the 4thday and rose back to 7.6 finally after 5 days, The residual sugar curve was decreased before stablising on the 6thday. It is presumed that a large amount of alkaline secondary metabolites might have been produced during fermentation. This study focuses on antagonism between aquatic pathogenic bacteria and fermentation metabolites from Antarctic fungi for the first time, which may provide data on research of antibiotics against aquatic pathogenic bacteria.

scholarly journals Extract from phyllosphere bacteria with antibiofilm and quorum quenching activity to control several fish pathogenic bacteria

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Olivia Nathalia ◽  
Diana Elizabeth Waturangi
Keyword(s):  
Streptococcus Agalactiae ◽  
Aeromonas Hydrophila ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Vibrio Harveyi ◽  
Quorum Quenching ◽  
Indicator Bacteria ◽  
Antibiofilm Activity ◽  
Fish Pathogen ◽  
Phyllosphere Bacteria

Abstract Objective The objective of this research were to screen quorum quenching activity compound from phyllosphere bacteria as well as antibiofilm activity against several fish pathogen bacteria such as Aeromonas hydrophila, Streptococcus agalactiae, and Vibrio harveyi. Results We found eight phyllosphere bacteria isolates with potential quorum quenching activity to inhibit Chromobacterium violaceum as indicator bacteria. Crude extracts (20 mg/mL) showed various antibiofilm activity against fish pathogenic bacteria used in this study. Isolate JB 17B showed the highest activity to inhibit biofilm formation of A. hydrophila and V. harveyi, meanwhile isolate JB 3B showed the highest activity to inhibit biofilm of S. agalactiae. From destruction assay, isolate JB 8F showed the highest activity to disrupt biofilm of A. hydrophila isolate JB 20B showed the highest activity to disrupt biofilm of V. harveyi, isolate JB 17B also showed the highest activity to disrupt biofilm of S. agalactiae.

scholarly journals Hemolysin, Protease, and EPS Producing PathogenicAeromonas hydrophilaStrain An4 Shows Antibacterial Activity against Marine Bacterial Fish Pathogens

2010 ◽  
Vol 2010 ◽  
pp. 1-9 ◽  
Author(s):  
Anju Pandey ◽  
Milind Naik ◽  
Santosh Kumar Dubey
Keyword(s):  
Antibacterial Activity ◽  
Aeromonas Hydrophila ◽  
Pathogenic Bacteria ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Antibacterial Properties ◽  
Cell Extract ◽  
Extracellular Proteins ◽  
Butylated Hydroxytoluene ◽  
Crude Cell Extract

A pathogenicAeromonas hydrophilastrain An4 was isolated from marine catfish and characterized with reference to its proteolytic and hemolytic activity along with SDS-PAGE profile (sodium dodecyl sulphate-Polyacrylamide gel electrophoresis) of ECPs (extracellular proteins) showing hemolysin (approximately 50 kDa). Agar well diffusion assay using crude cell extract of the bacterial isolate clearly demonstrated antibacterial activity against indicator pathogenic bacteria,Staphylococcus arlettaestrain An1,Acinetobactersp. strain An2,Vibrio parahaemolyticusstrain An3, andAlteromonas aurentiaSE3 showing inhibitory zone >10 mm well comparable to common antibiotics. Further GC-MS analysis of crude cell extract revealed several metabolites, namely, phenolics, pyrrolo-pyrazines, pyrrolo-pyridine, and butylated hydroxytoluene (well-known antimicrobials). Characterization of EPS using FTIR indicated presence of several protein-related amine and amide groups along with peaks corresponding to carboxylic and phenyl rings which may be attributed to its virulent and antibacterial properties, respectively. Besides hemolysin, EPS, and protease,Aeromonas hydrophilastrain An4 also produced several antibacterial metabolites.

scholarly journals Identification and Antibacterial Characterization of Endophytic Fungi from Artemisia sieberi

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Ibtisam Mohammed Ababutain ◽  
Sahar Khamees Aldosary ◽  
Amal Abdulaziz Aljuraifani ◽  
Azzah Ibrahim Alghamdi ◽  
Amira Hassan Alabdalall ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Molecular Identification ◽  
Endophytic Fungi ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Rrna Gene ◽  
Gram Positive ◽  
Artemisia Sieberi

Endophytic fungi serve as a reservoir for important secondary metabolites. The current study focused on the antibacterial properties of endophytic fungi isolated from Artemisia sieberi. Initially, six endophytic fungi were isolated and purified from the stem of A. sieberi. Endophytic fungi were identified by morphological characteristics, as well as by molecular identification using 18S rRNA gene sequencing method. All the six isolates were subjected to the preliminary screening for their antibacterial activity against nine important pathogenic bacteria using the disk-diffusion method. Crude extracts of the most active isolate were obtained using ethyl acetate. Antibacterial activity of the ethyl acetate extract was evaluated using well diffusion method on the selected isolate. The antibacterial efficiency of the selected isolate was evaluated by determining the Minimum Inhibitory Concentration (MIC). MIC values were in appreciable quantity against both Gram-positive and Gram-negative bacteria ranging from 3.125 to 6.25 µg/mL and 12.5 to 50 µg/mL, respectively. This result indicated that Gram-positive bacteria were more susceptible to the endophytic fungi extract. Moreover, the molecular identification results revealed that all the isolates belong to Ascomycota and represented Aspergillus and Penicillium genera and three species: A. oryzae (three isolates), A. niger (one isolate), and P. chrysogenum (two isolates). All six endophytic fungi were able to inhibit the growth of at least two of the tested bacteria. Among the isolated strains, isolate AS2, which identified as P. chrysogenum, exhibited the highest antibacterial activity against all nine tested bacteria and was higher than or equal to the positive control against most of the tested bacteria. Future studies are required to isolate and identify these bioactive substances, which can be considered as a potential source for the synthesis of new antibacterial drugs to treat infectious diseases.

scholarly journals EVALUATION OF ANTIBACTERIAL ACTIVITY OF ENDOPHYTIC FUNGI ASPERGILLUS JAPONICUS ISOLATED FROM TRIDAX PROCUMBENS L.

2018 ◽  
Vol 11 (9) ◽  
pp. 212 ◽  
Author(s):  
Ravindra Prasad Aharwal ◽  
Suneel Kumar ◽  
Yogita Thakur ◽  
Loknath Deshmukh ◽  
Sardul Singh Sandhu
Keyword(s):  
Antibacterial Activity ◽  
Solvent Extraction ◽  
Molecular Identification ◽  
Endophytic Fungi ◽  
Bacterial Strain ◽  
Pathogenic Bacteria ◽  
Fungal Strain ◽  
Fungal Isolate ◽  
Tridax Procumbens ◽  
Solvent Solvent

Objective: The main purpose of the present study was to isolate the endophytic fungi from Tridax procumbens L. and evaluate their antibacterial activity at different concentration of compatible solvents extracted metabolites against the test pathogenic bacterial strain, as well as the molecular characterization of potent endophytic fungal isolate that showed the maximum antibacterial activity.Methods: The endophytic fungi were isolated from the different parts of the collected T. procumbens plant. Screening of endophytic fungi for the antibacterial activity was scrutinized against five pathogenic bacteria such as Bacillus subtilis, Streptococcus pyogenes, Escherichia coli, Klebsiella pneumoniae, and Salmonella typhimurium using agar well diffusion method. After screening, the metabolite of the potent fungal isolate was extracted using different solvents by solvent-solvent extraction procedure and observed their antibacterial activity. For molecular identification of the fungi, the DNA was extracted, quantified, and amplified using two oligonucleotide primers ITS4 and ITS6 in polymerase chain reaction.Results: In the present study, five endophytic fungi were isolated from medicinal plant T. procumbens and screened for their antibacterial activity against E. coli (22.60±0.32 mm), S. typhimurium (19.26±0.23 mm), S. pyogenes (16.36±0.18 mm), K. pneumoniae (14.26±0.54 mm), and B. subtilis (14.43±0.27 mm) bacterial strain. The endophytic fungal strain A. japonicus isolated from T. procumbens was showed the significant antibacterial activity against the pathogenic bacteria. The morphological identification of all the isolated endophytic fungi was observed on the basis of their macroscopic and microscopic characteristics, and the molecular identification of the potent fungal strain was observed through 18s rRNA sequence analysis. Using solvent-solvent extraction technique, different solvent residues of the potent fungal metabolite were extracted in benzene, n-,butanol and toluene. The n-butanol solvent extract exhibited a maximum zone of inhibition against the test bacterial strains.Conclusion: The present study reveals that the endophytic fungi serve as a potential source for the production of effective bioactive compounds.

scholarly journals Antibacterial Alkaloids and Polyketide Derivatives from the Deep Sea-Derived Fungus Penicillium cyclopium SD-413

Marine Drugs ◽  
2020 ◽  
Vol 18 (11) ◽  
pp. 553
Author(s):  
Yan-He Li ◽  
Xiao-Ming Li ◽  
Xin Li ◽  
Sui-Qun Yang ◽  
Xiao-Shan Shi ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Deep Sea ◽  
Density Functional ◽  
Pathogenic Bacteria ◽  
Micrococcus Luteus ◽  
Vibrio Anguillarum ◽  
2D Nmr ◽  
Edwardsiella Tarda ◽  
Spectrometric Analysis ◽  
Mic Minimum Inhibitory Concentration

Nine secondary metabolites (1–9), including two new polyketide derivatives 9-dehydroxysargassopenilline A (4) and 1,2-didehydropeaurantiogriseol E (5), along with seven known related secondary metabolites (1–3 and 6–9), were isolated and identified from the deep sea-derived fungus Penicilliumcyclopium SD-413. Their structures were elucidated on the basis of 1D/2D NMR spectroscopic and mass spectrometric analysis and the absolute configurations were determined by the combination of NOESY correlations and time-dependent density functional (TDDFT) ECD calculations. Compounds 1–9 inhibited some pathogenic bacteria including Escherichia coli, E. ictaluri, Edwardsiella tarda, Micrococcus luteus, Vibrio anguillarum, and V. harveyi, with MIC (minimum inhibitory concentration) values ranging from 4 to 32 μg/mL.

scholarly journals Antibacterial activity of Actinomycetes symbiont with seaweeds: a prosperous agent of animal antibacterial

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Bertoka Fajar S.P. Negara ◽  
. Riyanti ◽  
Bintang Marhaeni ◽  
Aradea B. Kusuma
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Pathogenic Bacteria ◽  
Pcr Amplification ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
E Coli ◽  
Amplification Method ◽  
The Relationship

Circulation of synthetic antibacterial chemicals which is used continuously can cause resistance to bacteria. Alternative synthetic antibiotics research then comes up as an urgent need. Recently, the discovery of new antibacterial agents is looking for Actinomycetes. Actinomycetes are one of the active marine bacteria which produce secondary metabolites that could be used as antibacterial. The objective of this study was to evaluate the antibacterial activities of Actinomycetes symbiont with Halimeda macroloba, Gelidiella acerosa, and Turbinaria ornata in Pangandaran beach, Indonesia. A total of 41 Actinomycetes were isolated from 3 species of seaweed. Rep PCR amplification method was used to characterize and identify the relationship between samples. Primer BOX A1R (5’-CTACGGCAAGGCGACGCTGACGCTGACG-3’), 27F (5'-AGTTTGATCMTGGCTCAG-3') and 1492R (5'-TACGGYTACCTTGTTACGACTT-3') was used in amplification 16s DNA. Six isolates from the representative cluster dendogram were selected for secondary metabolites extraction using ethyl acetate solvent, meanwhile agar diffusion method was used as Antibacterial activity assay using E. coli, Proteus sp., and Enterobacter sp. as the bacterial test. The results showed that the activity of secondary metabolite extracts of Actinomycetes symbiont with Halimeda macroloba presented the highest inhibition zone on 3 bacterial tests. In the future, it could be a potential antibacterial agent against animal pathogenic bacteria.

scholarly journals Screening of Antibacterial Metabolites from Marine Soil, Kodiyampaiayam, Tamilnadu

2019 ◽  
Vol 8 (3S3) ◽  
pp. 571-575
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Drug Development ◽  
Marine Environment ◽  
Marine Sediment ◽  
Crude Extract ◽  
Pathogenic Bacteria ◽  
Soil Samples ◽  
Morphology Characteristic ◽  
New Antibiotics

The marine environment is a rich resource for isolating exploited microorganisms. In recent years, antibiotics have become important in this study of new antibiotics that show antiviral, anticoagulant and cardiac properties. Soil samples were collected from different area and isolated the bacterial colonies were separately streaking in agar plates (KP1, KP3, KP6, KP7 and KP9). The bacteria were done by morphology characteristic after that bacterial crude extract was taken separately. The crude extract was used in antibacterial activity against human pathogenic bacteria. There KP 7 and KP 9 havethe highest activity in B. subtilis and S. pyogenes rest of the samples also had activity comparing to these samples (KP 7 and KP 9) is highest zone inhibition developing. The marine sediment having so many microbes and secondary metabolites, therefore,the most useful drug development..

scholarly journals Therapeutic potentials of Excoecaria agallocha against gram-positive and gram-negative fish bacterial pathogens

2019 ◽  
Vol 2 (2) ◽  
pp. 87-94
Author(s):  
Laith A. Abdul Razak ◽  
Nadirah Musa ◽  
Aya Jabar ◽  
Najiah Musa
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Streptococcus Agalactiae ◽  
Oreochromis Niloticus ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Diffusion Method ◽  
Mangrove Plant ◽  
E Coli ◽  
Excoecaria Agallocha

Background: The present investigation of a mangrove plant, Excoecaria agallocha, which is a popular medicinal substitute for the treatment of microbial ailments, were evaluated for potential antimicrobial activity against pathogenic bacteria Escherichia coli and Streptococcus agalactiae in tilapia, Oreochromis niloticus. Methods: Antibacterial activity was performed using agar diffusion method, disc diffusion method, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and antibiotic susceptibility assays. Experimental fish fed diet containing 0 (control), 5, 25, and 50 mg kg-1 E. agallocha leaf methanol extract for 28 days then challenged individually with E. coli or S. agalactiae and mortalities were recorded over a ten-day post-infection period.  Results: Results indicated that both bacterial species are sensitive to tetracycline, ampicillin, and amoxicillin. E. coli was found to be resistant to neomycin. E. agallocha extract concentration of 50 mg/ml produced a zone of inhibition of 18 mm against E. coli, in contrast to 13 mm against S. agalactiae.  E. agallocha showed bactericidal activity against E. coli and bacteriostatic activity against S. agalactiae. The highest E. agallocha LC50 activity was 83 mg/ml. The highest cumulative mortality was 90.0 ± 10.0% in control as compared to 26.7 ± 11.5% in the group fed with 50 mg kg-1 E. agallocha extract, significant differences (P < 0.05). Conclusion: Hence, E. agallocha showed antibacterial activity against fish pathogens Escherichia coli and Streptococcus agalactiae in tilapia, Oreochromis niloticus; therefore, E. agallocha may be used as an alternative therapeutic agent against fish pathogenic bacteria as an additive to feed at a concentration depend, safe, non-cytotoxic doses.

scholarly journals The Antibacterial Activity and Phytochemical Compounds of Citrus grandis Extract Against Human Infective Bacteria

2020 ◽  
Vol 24 (3) ◽  
Author(s):  
Mostafa Alamholo ◽  
Mohaddeseh Shojaemehr
Keyword(s):  
Antibacterial Activity ◽  
Pharmaceutical Industry ◽  
Secondary Metabolites ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Total Phenol Content ◽  
White Skin ◽  
Phytochemical Compounds

Background: With the presence of secondary metabolites that have antimicrobial properties and bacterial resistance against antibiotics, medicinal plants have attracted the attention of researchers in the pharmaceutical industry. Objectives: The present study aimed to investigate the antibacterial and antioxidant activity and phytochemical compounds of Citrus grandis extract against human infection bacteria in-vitro. Methods: Samples of Citrus grandis were collected from the north of Iran and assessed in terms of the antibacterial activity, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) using the agar well diffusion method. In addition, the anti-radical activity was determined by DPPH, total phenols were measured using the Folin-Ciocalteu method, and flavonoids were determined using aluminum chloride. The presence of alkaloid, saponin, and tannin was also investigated. Data analysis was performed in SPSS. Results: The colored and white skin methanol extract of C. grandis indicated more significant inhibitory effects on Bacillus cereus, Staphylococcus aureus, and Micrococcus luteus. The total phenol content of the colored and white skin was estimated at 79.71 and 71.63 mgGA/g, and the flavonoid level was determined to be 3.63 and 4.06 mgQ/g, respectively. The IC50 of the colored and white skin methanol extract and ascorbic acid were estimated at 0.1251, 0.1376, and 0.1095 mg.mL-1, respectively. The methanol extract of the colored skin showed the presence of alkaloid, while the white skin showed the presence of alkaloid and saponin. Conclusions: According to the results, the C. grandis extract had antioxidant and antibacterial properties due to the presence of secondary metabolites. Therefore, it is recommended that natural and rare drugs be produced to control pathogenic bacteria in the pharmaceutical industry.

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