scholarly journals Quantification of Moss-Associated Cyanobacteria Using Phycocyanin Pigment Extraction

2021 ◽  
Vol 11 ◽  
Author(s):  
Marie Renaudin ◽  
Romain Darnajoux ◽  
Jean-Philippe Bellenger
Keyword(s):  
Boreal Forest ◽  
Environmental Changes ◽  
Matrix Effects ◽  
Anthropogenic Activities ◽  
High Recovery ◽  
Efficient Tool ◽  
Pigment Extraction ◽  
The Difference ◽  
Biological Nitrogen ◽  
Microscopic Counting

In the boreal forest, cyanobacteria can establish associations with feather moss and realize the biological nitrogen fixation (BNF) reaction, consisting in the reduction of atmospheric dinitrogen into bioavailable ammonium. In this ecosystem, moss-associated cyanobacteria are the main contributors to BNF by contributing up to 50% of new N input. Current environmental changes driven by anthropogenic activities will likely affect cyanobacteria activity (i.e., BNF) and populations inhabiting mosses, leading to potential important consequences for the boreal forest. Several methods are available to efficiently measure BNF activity, but quantifying cyanobacteria biomass associated with moss is challenging because of the difficulty to separate bacteria colonies from the host plant. Attempts to separate cyanobacteria by shaking or sonicating in water were shown to be poorly efficient and repeatable. The techniques commonly used, microscopic counting and quantitative PCR (qPCR) are laborious and time-consuming. In aquatic and marine ecosystems, phycocyanin (PC), a photosynthesis pigment produced by cyanobacteria, is commonly used to monitor cyanobacteria biomass. In this study, we tested if PC extraction and quantification can be used to estimate cyanobacteria quantity inhabiting moss. We report that phycocyanin can be easily extracted from moss by freeze/thaw disturbance of cyanobacteria cells and can be quickly and efficiently measured by spectrofluorometry. We also report that phycocyanin extraction is efficient (high recovery), repeatable (relative SD < 13%) and that no significant matrix effects were observed. As for aquatic systems, the main limitation of cyanobacteria quantification using phycocyanin is the difference of cellular phycocyanin content between cyanobacteria strains, suggesting that quantification can be impacted by cyanobacteria community composition. Nonetheless, we conclude that phycocyanin extraction and quantification is an easy, rapid, and efficient tool to estimate moss-associated cyanobacteria number.

scholarly journals Forecasting Agroforestry Ecosystem Services Provision in Urban Regeneration Projects: Experiences and Perspectives from Milan

2021 ◽  
Vol 13 (5) ◽  
pp. 2434 ◽  
Author(s):  
Ambrogio Zanzi ◽  
Federico Andreotti ◽  
Valentina Vaglia ◽  
Sumer Alali ◽  
Francesca Orlando ◽  
...  
Keyword(s):  
Ecosystem Services ◽  
Food System ◽  
Environmental Changes ◽  
Urban Environments ◽  
Urban Food System ◽  
Notable Increase ◽  
Living Fences ◽  
The Difference ◽  
Pollution Removal ◽  
The Relationship

The expansion of urban agglomerates is causing significant environmental changes, while the demand and need for sustainability keep on growing. In this context, urban and peri-urban agriculture can play a crucial role, mainly if associated with an agroecological approach. Indeed, the extensive use of living fences and tree rows can improve the environmental quality, assuring ecosystem services (ES), developing a sustainable urban food system and increasing local productions and the related socio-economic improvements. This study aims to assess the benefits of an agroecological requalification of a dismissed peri-urban area in the South Milan Agricultural Regional Park (Italy), by evaluating two possible scenarios, both involving planting trees and shrubs in that area. The software I-Tree Eco simulates the ecosystem services provision of planting new hedgerows, evaluating the benefits over 30 years. The study underlines the difference between the two scenarios and how the planted area becomes an essential supplier of regulating ecosystem services for the neighbourhoods, increasing carbon storage and air pollution removal. Results were then analysed with a treemap, to better investigate and understand the relationship between the different ecosystem services, showing a notable increase in carbon sequestration at the end of the simulation (at year 30). The study shows a replicable example of a methodology and techniques that can be used to assess the ES in urban and peri-urban environments.

scholarly journals Multi-domain probiotic consortium as an alternative to chemical remediation of oil spills at coral reefs and adjacent sites

Microbiome ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Denise P. Silva ◽  
Helena D. M. Villela ◽  
Henrique F. Santos ◽  
Gustavo A. S. Duarte ◽  
José Roberto Ribeiro ◽  
...  
Keyword(s):  
Microbial Community ◽  
Coral Reefs ◽  
Environmental Changes ◽  
Oil Spills ◽  
Anthropogenic Activities ◽  
Oil Contamination ◽  
Negative Effects ◽  
Corexit 9500 ◽  
Coral Health ◽  
The Impact

Abstract Background Beginning in the last century, coral reefs have suffered the consequences of anthropogenic activities, including oil contamination. Chemical remediation methods, such as dispersants, can cause substantial harm to corals and reduce their resilience to stressors. To evaluate the impacts of oil contamination and find potential alternative solutions to chemical dispersants, we conducted a mesocosm experiment with the fire coral Millepora alcicornis, which is sensitive to environmental changes. We exposed M. alcicornis to a realistic oil-spill scenario in which we applied an innovative multi-domain bioremediator consortium (bacteria, filamentous fungi, and yeast) and a chemical dispersant (Corexit® 9500, one of the most widely used dispersants), to assess the effects on host health and host-associated microbial communities. Results The selected multi-domain microbial consortium helped to mitigate the impacts of the oil, substantially degrading the polycyclic aromatic and n-alkane fractions and maintaining the physiological integrity of the corals. Exposure to Corexit 9500 negatively impacted the host physiology and altered the coral-associated microbial community. After exposure, the abundances of certain bacterial genera such as Rugeria and Roseovarius increased, as previously reported in stressed or diseased corals. We also identified several bioindicators of Corexit 9500 in the microbiome. The impact of Corexit 9500 on the coral health and microbial community was far greater than oil alone, killing corals after only 4 days of exposure in the flow-through system. In the treatments with Corexit 9500, the action of the bioremediator consortium could not be observed directly because of the extreme toxicity of the dispersant to M. alcicornis and its associated microbiome. Conclusions Our results emphasize the importance of investigating the host-associated microbiome in order to detect and mitigate the effects of oil contamination on corals and the potential role of microbial mitigation and bioindicators as conservation tools. Chemical dispersants were far more damaging to corals and their associated microbiome than oil, and should not be used close to coral reefs. This study can aid in decision-making to minimize the negative effects of oil and dispersants on coral reefs.

Middle-to late Holocene environmental changes based on a multi-proxy lagoonal record, Klisova lagoon, Greece

2021 ◽  
Author(s):  
Alexandros Emmanouilidis ◽  
Konstantinos Panagiotopoulos ◽  
Katerina Kouli ◽  
Pavlos Avramidis
Keyword(s):  
Radiocarbon Dating ◽  
Environmental Changes ◽  
Eastern Mediterranean ◽  
Anthropogenic Activities ◽  
Eastern Mediterranean Region ◽  
Anthropogenic Activity ◽  
Palynological Analysis ◽  
Drilling Site ◽  
Geomorphological Changes ◽  
Shallow Water Depth

<p>Coastal wetlands are dynamic environments prone to climatic and anthropogenic forcing and ideal settings to study past climatic and environmental changes.  In the eastern Mediterranean region and particularly in Greece, the climate presents high spatiotemporal diversity, while human activity is a significant factor in shaping the landscape. This study presents a sediment record from Klisova lagoon, situated in central Greece, at the eastern part of Messolonghi lagoon complex. The area is recorded from antiquity to have great anthropogenic activity. The paleoenvironmental synthesis was based on standard sedimentological analysis (grain size, TOC, magnetic susceptibility), joint micropaleontological and palynological analysis, X-ray Fluorescence scanning, and radiocarbon dating. The Bayesian age-depth model is based on radiocarbon dating and yields an age of 4700 cal BP for the base of the recovered sediment sequence. For the last 4700 years, the freshwater influx, the progradation of the Evinos river delta and related geomorphological changes control the environmental conditions (e.g. depth and salinity) in the lagoon system. Prior to 4000 cal BP, a relatively shallow water depth, significant terrestrial/freshwater input and increased weathering in the lagoon area are inferred. Elemental proxies and increased dinoflagellate and foraminifera abundances, which indicate marine conditions with prominent freshwater influxes, point to the gradual deepening of the lagoon recorded at the drilling site up to 2000 cal BP. The marine and freshwater conditions equilibrium sets at 1300 cal BP, and the lagoonal system seems to reach its present state. Maxima of anthropogenic pollen indicators during the Mycenaean (~3200 cal BP), Hellenistic (~ 2200 cal BP) and Late Byzantine (~ 800 cal BP) periods suggest intervals of increased anthropogenic activities in the study area.  </p>

Late Quaternary vegetation dynamics in a biodiversity hotspot, the Uluguru Mountains of Tanzania

2009 ◽  
Vol 72 (1) ◽  
pp. 111-122 ◽  
Author(s):  
Jemma Finch ◽  
Melanie J. Leng ◽  
Rob Marchant
Keyword(s):  
Environmental Changes ◽  
Vegetation History ◽  
Late Quaternary ◽  
Anthropogenic Activities ◽  
Significant Loss ◽  
Environmental Stability ◽  
Forest Composition ◽  
Eastern Arc ◽  
Uluguru Mountains

AbstractLate Quaternary vegetation history and environmental changes in a biodiverse tropical ecosystem are inferred from pollen, charcoal and carbon isotope evidence derived from a ∼ 48,000-yr sedimentary record from the Uluguru Mountains, a component of the Eastern Arc Mountains of Kenya and Tanzania. Results indicate that Eastern Arc forest composition has remained relatively stable during the past ∼ 48,000 yr. Long-term environmental stability of the Eastern Arc forests has been proposed as a mechanism for the accumulation and persistence of species during glacial periods, thus resulting in the diverse forests observed today. The pollen and isotope data presented here indicate some marked changes in abundance but no significant loss in moist forest taxa through the last glacial maximum, thereby providing support for the long-term environmental stability of the Eastern Arc. Anthropogenic activities, including burning and forest clearance, were found to play a moderate role in shaping the mosaic of forest patches and high-altitude grasslands that characterise the site today; however, this influence was tempered by the inaccessibility of the mountain.

scholarly journals Lake water dissolved inorganic carbon dynamics revealed from monthly measurements of radiocarbon in the Fuji Five Lakes, Japan

Elem Sci Anth ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Kosuke Ota ◽  
Yusuke Yokoyama ◽  
Yosuke Miyairi ◽  
Shinya Yamamoto ◽  
Toshihiro Miyajima
Keyword(s):  
Lake Water ◽  
Inorganic Carbon ◽  
Environmental Changes ◽  
Dissolved Inorganic Carbon ◽  
Anthropogenic Activities ◽  
Aquatic Organisms ◽  
Human Society ◽  
History Of ◽  
Lake Waters ◽  
Reservoir Age

Lakes are sensitive recorders of anthropogenic activities, as human society often develops in their vicinity. Lake sediments thus have been widely used to reconstruct the history of environmental changes in the past, anthropogenic, or otherwise, and radiocarbon dating provides chronological control of the samples. However, specific values of radiocarbon in different carbon reservoirs due to the different pathways of radiocarbon from the upper atmosphere to the lake, called the radiocarbon reservoir age, is always difficult to evaluate because of dynamic processes in and around lakes. There are few systematic studies on radiocarbon reservoir ages for lakes owing to the complex radiocarbon transfer processes for lakes. Here, we investigate lake waters of the Fuji Five Lakes with monthly monitoring of the radiocarbon reservoir effects. Radiocarbon from dissolved inorganic carbon (DIC) for groundwater and river water is also measured, with resulting concentrations (Δ14C) at their lowest at Lake Kawaguchi in August 2018 (–122.4 ± 3.2‰), and at their highest at Lake Motosu in January 2019 (–22.4 ± 2.5‰), despite a distance of 25 km. However, winter values in both lakes show similar trends of rising Δ14C (about 20‰). Our lake water DIC Δ14C results are compared to previously published records obtained from sediments in Lake Motosu and Lake Kawaguchi. These suggest that total organic carbon and compound-specific radiocarbon found in sediments are heavily influenced by summer blooms of aquatic organisms that fix DIC in water. Thus, future studies to conduct similar analyses at the various lakes would be able to provide further insights into the carbon cycle around inland water, namely understanding the nature of radiocarbon reservoir ages.

scholarly journals Shallow Groundwater Quality Assessment and Its Suitability Analysis for Drinking and Irrigation Purposes

Water ◽  
2021 ◽  
Vol 13 (23) ◽  
pp. 3361
Author(s):  
Muhammad Yousuf Jat Baloch ◽  
Wenjing Zhang ◽  
Juanfen Chai ◽  
Shuxin Li ◽  
Muwaffaq Alqurashi ◽  
...  
Keyword(s):  
Quality Assessment ◽  
Environmental Changes ◽  
Carbonic Acid ◽  
Anthropogenic Activities ◽  
Hazard Index ◽  
Shallow Groundwater ◽  
The United States ◽  
Chloride Ions ◽  
Human Consumption ◽  
Water Type

For shallow groundwater, hydrogeochemical processes and quality assessment must be addressed because shallow groundwater is freely available in many parts of the globe. Due to recent anthropogenic activities and environmental changes in Sakrand, Sindh, Pakistan, the groundwater is extremely vulnerable. To provide safe drinking and agricultural water, hydrogeochemical analysis is required. Ninety-five groundwater samples were analyzed using agricultural and drinking indices to determine the hydrogeochemical parameters using multivariate analysis such as Pearson correlations, principal component cluster analysis, as well as Piper diagrams and Gibbs plot for drinking and agricultural indices. An abundance of ions was observed through the statistical summary; however, cations and anions were recorded in the orders Na+ > Ca2+ > Mg2+ > K+ and HCO3− > Cl− > SO42− > NO3− > F−. The hydrogeochemical process used to quantify the major reactions occurring in the groundwater system showed rock dominance; the Piper diagrams evaluated the water type. A mixed pattern of calcium, magnesium, and chloride ions (Ca2+−Mg2+−Cl− type) was observed. Additionally, the ion exchange method showed an excess of bicarbonate ions due to carbonic acid weathering. The water quality index (WQI) resulted 32.6% of groundwater being unsuitable for human consumption; however, the United States Salinity Laboratory (USSL) diagram showed 60% of samples fall in the irrigable category and the Wilcox diagram depicted 5% of samples lying in the unsuitable region. Most of the water samples were suitable for drinking; only a few samples were unsafe for drinking purposes for children due to the high hazard index. High salinity meant some samples were unsuitable for irrigation.

The 2018 heatwave and its implications on ozone induced damage on vegetation in a subarctic climate

2021 ◽  
Author(s):  
Stefanie Falk ◽  
Ane Victoria Vollsnes ◽  
Lisa Emberson ◽  
Connie O'Neill ◽  
Aud Else Berglen Eriksen ◽  
...  
Keyword(s):  
Damage Assessment ◽  
Environmental Changes ◽  
Ambient Air ◽  
Arctic Climate ◽  
Test Case ◽  
Future Scenario ◽  
Ozone Concentrations ◽  
Subarctic Climate ◽  
The Difference ◽  
Favorable Conditions

<p>An increased occurrence of persistent heatwaves, as one possible future scenario, generates favorable conditions for the formation of ambient air ozone. Vegetation highly specialized to sub-arctic climate is vulnerable to rapid environmental changes inflicted by global warming and might become more susceptible to ozone in the future. Over large parts of Europe the summer 2018 had been extraordinarily hot and dry and caused large wildfires in northern Sweden in particular. This can be regarded as a test case for such a future scenario. In both 2018 and 2019, we have monitored ambient air ozone concentrations at the Norwegian Institute of Bioeconomy Research (NIBIO) Environment Centre Svanhovd in Northern Norway. Due to<br>data acquisition problems, ozone concentrations for two weeks in July 2018 were missing from our record. We present a reconstruction based on probability density function with respect to the Swedish and Finnish atmospheric monitoring sites in the region. Over all, ozone concentrations did not differ significantly between the two years. While temperatures and global irradiance diverged significantly from multi annual mean, precipitation varied only to some extend. Coincidentally, we have observed ozone-induced visible injuries on clovers in the ozone garden at Svanhovd in 2018, but not in 2019. We investigate the difference in uptake of ozone using the DO3SE model, with respect to the typical vegetation (e.g., birch and conifers) at<br>the location. We assess whether critical levels on POD1 for these species were breached. We find that an unadjusted transfer of currently used standard parameters and methodes on ozone damage assessment (IPC Mapping Manual) to vegetation in the subarctics will result in an missinterpretation of POD1 values.</p>

Microbial Diversity in the Glacial Ecosystem of Antarctic, Arctic, and Tibetan Plateau: Properties and Response to the Environmental Condition

2020 ◽  
Vol 9 (1) ◽  
pp. 231-250
Author(s):  
Birendra Prasad Sharma ◽  
Subash Adhikari ◽  
Ganesh Paudel ◽  
Namita Paudel Adhikari
Keyword(s):  
Tibetan Plateau ◽  
Microbial Diversity ◽  
Bacterial Communities ◽  
Environmental Changes ◽  
Anthropogenic Activities ◽  
Relevant Literature ◽  
The Tibetan Plateau ◽  
Polar Regions ◽  
Cold Temperatures ◽  
Bacterial Phyla

Microorganisms, as successive members of the food web, play a major role in biological processes. They are found in environments ranging from extremely hot to harsh cold temperatures. Thus, the study of bacterial communities in various ecosystems is of great concern around the world. The glacier is one of the parts of the cryosphere, which is the key component and sensitive indicator of climatic and environmental changes. A glacial ecosystem is a habitat for various microorganisms, i.e., autotrophic and heterotrophic. Different physicochemical parameters like temperature, pH, electrical conductivity, the input of nutrient concentration, precipitation, ions concentrations, etc. influence the microbial diversity in the glacial ecosystem for their metabolic processes. Successive studies of bacterial communities in the Himalayan glacial ecosystem are reliable proxies to know the relationships between microbial biodiversity and climate change since the Himalayan glaciers are free from anthropogenic activities. After the study of the relevant literature, it is clear that the researches. have been carried out in the Polar Regions, and the Tibetan plateau mainly focused on the glacial ecosystem. This review concluded that Proteobacteria, Bacteroidetes, Cyanobacteria, Firmicutes, Verrucomicrobia, and Actinobacteria were the most dominant bacterial phyla via 16S rRNA clone libraries and Illumina MiSeq. Alter in landscapes, nutrient cycles, exposure of light, shifting on the concentration of different elements, glacier retreats were the major components for survival strength of dominant bacterial phyla. However, limited studies on the glacial ecosystem of the Himalayas have been published. Thus, the study of bacterial abundance, diversity, and community in the Himalayas will help plug this research gap.

Effects of lyophilization of serum on the measurement of apolipoproteins A-I and B

1990 ◽  
Vol 36 (2) ◽  
pp. 366-369 ◽  
Author(s):  
S M Marcovina ◽  
J L Adolphson ◽  
M Parlavecchia ◽  
J J Albers
Keyword(s):  
Reference Materials ◽  
Apolipoprotein B ◽  
Matrix Effects ◽  
Reference Intervals ◽  
Assay Method ◽  
Common Reference ◽  
Apolipoprotein A ◽  
Quality Control Materials ◽  
Standardization Process ◽  
The Difference

Abstract A common accuracy-based standardization program is indispensable for establishing reference intervals for the clinical use of apolipoproteins. The development and distribution of reference materials and quality-control materials that do not exhibit matrix effects between methods is essential to the standardization process. We examined the suitability of lyophilized material as a common reference material for the measurement of apolipoproteins A-I and B. We determined values for apolipoproteins A-I and B in frozen and lyophilized serum pools, using different immunochemical approaches. We found little or no differences in apolipoprotein A-I values between frozen and lyophilized pools as determined by the different methods. In contrast, values for apolipoprotein B in lyophilized samples were consistently lower than those obtained for frozen samples. After adjusting for the effect of dilution due to reconstitution, the difference in the apolipoprotein B values for lyophilized as compared with frozen samples ranged from -26% to 4%, depending upon the assay method. Evidently, serum pools in lyophilized from are not a suitable matrix for reference materials for apolipoprotein B measurements but can be used for apolipoprotein A-I measurements.

scholarly journals The role of vegetation in the CO2 flux from a tropical urban neighbourhood

2013 ◽  
Vol 13 (20) ◽  
pp. 10185-10202 ◽  
Author(s):  
E. Velasco ◽  
M. Roth ◽  
S. H. Tan ◽  
M. Quak ◽  
S. D. A. Nabarro ◽  
...  
Keyword(s):  
Soil Respiration ◽  
Anthropogenic Activities ◽  
Co2 Flux ◽  
Accurate Estimation ◽  
Urban Vegetation ◽  
Co2 Uptake ◽  
Total Contribution ◽  
The Difference ◽  
Residential Neighbourhood

Abstract. Urban surfaces are usually net sources of CO2. Vegetation can potentially have an important role in reducing the CO2 emitted by anthropogenic activities in cities, particularly when vegetation is extensive and/or evergreen. A direct and accurate estimation of carbon uptake by urban vegetation is difficult due to the particular characteristics of the urban ecosystem and high variability in tree distribution and species. Here, we investigate the role of urban vegetation in the CO2 flux from a residential neighbourhood in Singapore using two different approaches. CO2 fluxes measured directly by eddy covariance are compared with emissions estimated from emissions factors and activity data. The latter includes contributions from vehicular traffic, household combustion, soil respiration and human breathing. The difference between estimated emissions and measured fluxes should approximate the flux associated with the aboveground vegetation. In addition, a tree survey was conducted to estimate the annual CO2 sequestration using allometric equations and an alternative model of the metabolic theory of ecology for tropical forests. Palm trees, banana plants and turfgrass were also included in the survey with their annual CO2 uptake obtained from published growth rates. Both approaches agree within 2% and suggest that vegetation sequesters 8% of the total emitted CO2 in the residential neighbourhood studied. An uptake of 1.4 ton km−2 day−1 (510 ton km−2 yr−1) was estimated as the difference between assimilation by photosynthesis minus the aboveground biomass respiration during daytime (4.0 ton km−2 day−1) and release by plant respiration at night (2.6 ton km−2 day−1). However, when soil respiration is added to the daily aboveground flux, the biogenic component becomes a net source amounting to 4% of the total CO2 flux and represents the total contribution of urban vegetation to the carbon flux to the atmosphere.

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