scholarly journals Distinct Endophytic Bacterial Communities Inhabiting Seagrass Seeds

2021 ◽  
Vol 12 ◽  
Author(s):  
Flavia Tarquinio ◽  
Océane Attlan ◽  
Mathew A. Vanderklift ◽  
Oliver Berry ◽  
Andrew Bissett
Keyword(s):  
Female Flower ◽  
Amplicon Sequencing ◽  
Terrestrial Plants ◽  
Pathogenic Potential ◽  
Core Microbiome ◽  
Reproductive Tissues ◽  
Halophila Ovalis ◽  
Plant Growth Promoting ◽  
Potential Activity ◽  
Growth Promoting

Seagrasses are marine angiosperms that can live completely or partially submerged in water and perform a variety of significant ecosystem services. Like terrestrial angiosperms, seagrasses can reproduce sexually and, the pollinated female flower develop into fruits and seeds, which represent a critical stage in the life of plants. Seed microbiomes include endophytic microorganisms that in terrestrial plants can affect seed germination and seedling health through phytohormone production, enhanced nutrient availability and defence against pathogens. However, the characteristics and origins of the seagrass seed microbiomes is unknown. Here, we examined the endophytic bacterial community of six microenvironments (flowers, fruits, and seeds, together with leaves, roots, and rhizospheric sediment) of the seagrass Halophila ovalis collected from the Swan Estuary, in southwestern Australia. An amplicon sequencing approach (16S rRNA) was used to characterize the diversity and composition of H. ovalis bacterial microbiomes and identify core microbiome bacteria that were conserved across microenvironments. Distinct communities of bacteria were observed within specific seagrass microenvironments, including the reproductive tissues (flowers, fruits, and seeds). In particular, bacteria previously associated with plant growth promoting characteristics were mainly found within reproductive tissues. Seagrass seed-borne bacteria that exhibit growth promoting traits, the ability to fix nitrogen and anti-pathogenic potential activity, may play a pivotal role in seed survival, as is common for terrestrial plants. We present the endophytic community of the seagrass seeds as foundation for the identification of potential beneficial bacteria and their selection in order to improve seagrass restoration.

Community dynamics of duckweed-associated bacteria upon inoculation of plant growth-promoting bacteria

2020 ◽  
Vol 96 (7) ◽  
Author(s):  
Hidehiro Ishizawa ◽  
Masashi Kuroda ◽  
Daisuke Inoue ◽  
Masaaki Morikawa ◽  
Michihiko Ike
Keyword(s):  
Plant Growth ◽  
Bacterial Communities ◽  
Production Efficiency ◽  
Community Dynamics ◽  
Amplicon Sequencing ◽  
Plant Colonization ◽  
Plant Growth Promoting Bacteria ◽  
Short Period ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACT Plant growth-promoting bacteria (PGPB) have recently been demonstrated as a promising agent to improve wastewater treatment and biomass production efficiency of duckweed hydrocultures. With a view to their reliable use in aqueous environments, this study analysed the plant colonization dynamics of PGPB and the ecological consequences for the entire duckweed-associated bacterial community. A PGPB strain, Aquitalea magnusonii H3, was inoculated to duckweed at different cell densities or timings in the presence of three environmental bacterial communities. The results showed that strain H3 improved duckweed growth by 11.7–32.1% in five out of nine experiments. Quantitative-PCR and amplicon sequencing analyses showed that strain H3 successfully colonized duckweed after 1 and 3 d of inoculation in all cultivation tests. However, it significantly decreased in number after 7 d, and similar bacterial communities were observed on duckweed regardless of H3 inoculation. Predicted metagenome analysis suggested that genes related to bacterial chemotactic motility and surface attachment systems are consistently enriched through community assembly on duckweed. Taken together, strain H3 dominantly colonized duckweed for a short period and improved duckweed growth. However, the inoculation of the PGPB did not have a lasting impact due to the strong resilience of the natural duckweed microbiome.

scholarly journals Identification and Differentiation of Pseudomonas Species in Field Samples Using an rpoD Amplicon Sequencing Methodology

mSystems ◽  
2021 ◽  
Author(s):  
Jonas Greve Lauritsen ◽  
Morten Lindqvist Hansen ◽  
Pernille Kjersgaard Bech ◽  
Lars Jelsbak ◽  
Lone Gram ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Amplicon Sequencing ◽  
Rrna Gene ◽  
Content Type ◽  
Field Samples ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Rrna Gene Sequencing ◽  
Specific Species

A high-throughput sequencing-based method for profiling of Pseudomonas species in soil microbiomes was developed and identified more species than 16S rRNA gene sequencing or cultivation. Pseudomonas species are used as biocontrol organisms and plant growth-promoting agents, and the method will allow tracing of specific species of Pseudomonas as well as enable screening of environmental samples for further isolation and exploitation.

scholarly journals Large Rhizosphere Bacterial Diversity Exits Among Wild Progenitor Species of Modern Sugarcane (Saccharum Spp. Inter-Specific Hybrids)

2021 ◽  
Author(s):  
Mukesh Kumar Malviya ◽  
Chnag-Ning Li ◽  
Manoj Kumar Solanki ◽  
Prakash Lakshmanan ◽  
Rajesh Kumar Singh ◽  
...  
Keyword(s):  
Plant Growth ◽  
Bacterial Diversity ◽  
Bacterial Communities ◽  
Wild Progenitor ◽  
Core Microbiome ◽  
Saccharum Spp ◽  
Operational Taxonomic Units ◽  
Plant Growth Promoting ◽  
Related Wild Species ◽  
Growth Promoting

Abstract BackgroundRhizosphere is rich in highly diverse and complex microbial communities. Plant growth promoting rhizpbacteria and diazotrops are played crucial role in plant growth and development. In this study, rhizosphere soils were collected from five wild Saccharum species- S. officinarum L. cv Badila (BRS), S. barberi Jesw. cv Pansahi (PRS), S. robustum (RRS), S. spontaneum (SRS), and S. sinense Roxb. cv Uba (URS) for studied of rhizosphere and diazotroph bacterial diversity using 16S rRNA and nifH gene amplification and sequencing.ResultsWe detected a total of 6202 operational taxonomic units (OTUs) specific to the bacterial communities from all species combined. Out of the 107 bacterial communities detected among all samples, we found a core microbiome of 31 rhizobacterial families spread across all the species analyzed. A total of 1099 OTUs were identified for diazotrophs with a core microbiome of 9 families distributed among all the sugarcane species. The core microbiomes were distributed across twenty genera-Bradyrhizobium, Dechloromonas, Desulfovibrio, Stenotrophomonas, Xanthobacter, Anaeromyxobacter, Azospirillum, Pseudoacidovorax, Methylobacterium, Azoarcus, Paenibacillus, Ideonella, Beijerinckia, Paraburkholderia, Burkholderia, Ruficoccus, Geobacter, Sinorhizobium, Kosakonia, and Azotobacter. ConclusionThe results presented here advance our understanding of rhizosphere associated bacterial diversity among genetically closely related wild species and provide a knowledge base for studying the evolution of rhizobacteria-host plant association during crop domestication.

scholarly journals Arbuscular Mycorrhizal Fungi in Agriculture

Encyclopedia ◽  
2021 ◽  
Vol 1 (4) ◽  
pp. 1132-1154
Author(s):  
Thomas I. Wilkes
Keyword(s):  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Soil Disturbance ◽  
Terrestrial Plants ◽  
Fungal Host ◽  
Tillage Practices ◽  
Plant Growth Promoting ◽  
Am Fungus ◽  
Growth Promoting

Arbuscular mycorrhizal (AM) fungi are biotrophic symbionts forming close relationships with an estimated 80% of terrestrial plants suitable as their host. Via an established AM fungal–host relationship, soil-bound nutrients are made available to the host plant through root cortical arbuscules as the site of exchange. At these sites, photosynthetic carbohydrates are provided to the AM fungus—carbohydrates that cannot be produced by the fungus. AM fungal–host symbiosis is very sensitive to soil disturbance, for example, agricultural tillage practices can damage and reduce AM fungal abilities to interact with a host and provide plant growth-promoting properties.

scholarly journals Capacity of soil bacteria to reach the phyllosphere and convergence of floral communities despite soil microbiota variation

2021 ◽  
Vol 118 (41) ◽  
pp. e2100150118
Author(s):  
Julien Massoni ◽  
Miriam Bortfeld-Miller ◽  
Alex Widmer ◽  
Julia A. Vorholt
Keyword(s):  
Microbial Communities ◽  
Plant Pathogens ◽  
Relative Proportion ◽  
Environmental Filtering ◽  
Common Garden ◽  
Amplicon Sequencing ◽  
Reproductive Organs ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Common Garden Experiments

Leaves and flowers are colonized by diverse bacteria that impact plant fitness and evolution. Although the structure of these microbial communities is becoming well-characterized, various aspects of their environmental origin and selection by plants remain uncertain, such as the relative proportion of soilborne bacteria in phyllosphere communities. Here, to address this issue and to provide experimental support for bacteria being filtered by flowers, we conducted common-garden experiments outside and under gnotobiotic conditions. We grew Arabidopsis thaliana in a soil substitute and added two microbial communities from natural soils. We estimated that at least 25% of the phyllosphere bacteria collected from the plants grown in the open environment were also detected in the controlled conditions, in which bacteria could reach leaves and flowers only from the soil. These taxa represented more than 40% of the communities based on amplicon sequencing. Unsupervised hierarchical clustering approaches supported the convergence of all floral microbiota, and 24 of the 28 bacteria responsible for this pattern belonged to the Burkholderiaceae family, which includes known plant pathogens and plant growth-promoting members. We anticipate that our study will foster future investigations regarding the routes used by soil microbes to reach leaves and flowers, the ubiquity of the environmental filtering of Burkholderiaceae across plant species and environments, and the potential functional effects of the accumulation of these bacteria in the reproductive organs of flowering plants.

Aplikasi Bacillus subtilis pada beberapa Bahan Organik terhadap Pertumbuhan dan Produksi Tanaman Cabai Rawit (Capsicum frutescens L.)

2020 ◽  
Vol 21 (1) ◽  
pp. 14-19
Author(s):  
Praptiningsih Gamawati Adinurani ◽  
Sri Rahayu ◽  
Nurul Fima Zahroh
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Capsicum Frutescens ◽  
Plant Growth Promoting ◽  
Growth Promoting

Mikroba Bacillus subtilis merupakan agen pengendali hayati mempunyai kelebihan sebagai Plant Growth Promoting Rhizobacteria (PGPR) yaitu dapat berfungsi sebagai biofertilizer, biostimulan, biodekomposer dan bioprotektan. Tujuan penelitian mengetahui potensi B. subtilis dalam merombak bahan organik sebagai usaha meningkatkan ketersediaan bahan organik tanah yang semakin menurun. Penelitian menggunakan Rancangan Petak Terbagi dengan berbagai  bahan organik sebagai petak utama (B0 = tanpa bahan organik, B1 = kotoran ayam,  B2 = kotoran kambing, B3 = kotoran sapi) dan aplikasi B.subtilis sebagai anak petak (A0 = 0 cc/L, A1 = 5cc/L, A2 = 10 cc/L, Pengamatan meliputi variabel tinggi tanaman, indeks luas daun, jumlah buah per tanaman, berat buah per tanaman, dan bahan organik tanah. Data pengamatan  dianalisis ragam  menggunakan  Statistical Product and Service Solutions (SPSS) versi 25 dan dilanjutkan dengan uji Duncan untuk mengetahui signifikansi perbedaan antar perlakuan. Hasil penelitian menunjukkan tidak terdapat interaksi antara bahan organik kotoran ternak dan konsentrasi B. subtilis terhadap semua variabel pengamatan. Potensi B. subtilis sangat baik dalam mendekomposisi bahan organik yang ditunjukkan dengan peningkatan bahan organik, dan hasil terbaik pada kotoran  sapi (B3) dan konsentrasi B. subtilis 15 mL/L masing-masing sebesar 46.47 % dan 34.76 %. Variabel pertumbuhan tidak berbeda nyata kecuali tinggi tanaman dengan pertambahan tinggi paling banyak pada pemberian kotoran kambing sebesar 170.69 %.

Isolation and identification of plant growth-promoting bacteria associated with tall fescue

2009 ◽  
pp. 211-216 ◽  
Author(s):  
J. Monk ◽  
E. Gerard ◽  
S. Young ◽  
K. Widdup ◽  
M. O'Callaghan
Keyword(s):  
New Zealand ◽  
Plant Growth ◽  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Plant Growth Promoting Bacteria ◽  
Beneficial Bacteria ◽  
Isolation And Identification ◽  
Naturally Occurring ◽  
Plant Growth Promoting ◽  
Growth Promoting

Tall fescue (Festuca arundinacea) is a useful alternative to ryegrass in New Zealand pasture but it is slow to establish. Naturally occurring beneficial bacteria in the rhizosphere can improve plant growth and health through a variety of direct and indirect mechanisms. Keywords: rhizosphere, endorhiza, auxin, siderophore, P-solubilisation

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