Genome-Wide Characterization of bHLH Genes in Grape and Analysis of their Potential Relevance to Abiotic Stress Tolerance and Secondary Metabolite Biosynthesis

AbstractHops are valued for their secondary metabolites, including bitter acids, flavonoids, oils, and polyphenols, that impart flavor in beer. Previous studies have shown that hop yield and bitter acid content decline with increased temperatures and low-water stress. We looked at physiological traits and differential gene expression in leaf, stem, and root tissue from hop (Humulus lupulus) cv. USDA Cascade in plants exposed to high temperature stress, low-water stress, and a compound treatment of both high temperature and low-water stress for six weeks. The stress conditions imposed in these experiments caused substantial changes to the transcriptome, with significant reductions in the expression of numerous genes involved in secondary metabolite biosynthesis. Of the genes involved in bitter acid production, the critical gene valerophenone synthase (VPS) experienced significant reductions in expression levels across stress treatments, suggesting stress-induced lability in this gene and/or its regulatory elements may be at least partially responsible for previously reported declines in bitter acid content. We also identified a number of transcripts with homology to genes shown to affect abiotic stress tolerance in other plants that may be useful as markers for breeding improved abiotic stress tolerance in hop. Lastly, we provide the first transcriptome from hop root tissue.

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Genome-Wide Identification and Expression Profiling of the PDI Gene Family Reveals Their Probable Involvement in Abiotic Stress Tolerance in Tomato (Solanum lycopersicum L.)

Genes ◽

10.3390/genes12010023 ◽

2020 ◽

Vol 12 (1) ◽

pp. 23

Author(s):

Antt Htet Wai ◽

Muhammad Waseem ◽

A B M Mahbub Morshed Khan ◽

Ujjal Kumar Nath ◽

Do Jin Lee ◽

...

Keyword(s):

Abiotic Stress ◽

Gene Family ◽

Stress Tolerance ◽

Solanum Lycopersicum ◽

Expression Profiling ◽

Abiotic Stress Tolerance ◽

Dependent Manner ◽

Solanum Lycopersicum L ◽

Genome Wide ◽

Protein Disulfide

Protein disulfide isomerases (PDI) and PDI-like proteins catalyze the formation and isomerization of protein disulfide bonds in the endoplasmic reticulum and prevent the buildup of misfolded proteins under abiotic stress conditions. In the present study, we conducted the first comprehensive genome-wide exploration of the PDI gene family in tomato (Solanum lycopersicum L.). We identified 19 tomato PDI genes that were unevenly distributed on 8 of the 12 tomato chromosomes, with segmental duplications detected for 3 paralogous gene pairs. Expression profiling of the PDI genes revealed that most of them were differentially expressed across different organs and developmental stages of the fruit. Furthermore, most of the PDI genes were highly induced by heat, salt, and abscisic acid (ABA) treatments, while relatively few of the genes were induced by cold and nutrient and water deficit (NWD) stresses. The predominant expression of SlPDI1-1, SlPDI1-3, SlPDI1-4, SlPDI2-1, SlPDI4-1, and SlPDI5-1 in response to abiotic stress and ABA treatment suggested they play regulatory roles in abiotic stress tolerance in tomato in an ABA-dependent manner. Our results provide new insight into the structure and function of PDI genes and will be helpful for the selection of candidate genes involved in fruit development and abiotic stress tolerance in tomato.

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Molecular Characterization of a MYB Protein from Oryza sativa for its Role in Abiotic Stress Tolerance

Brazilian Archives of Biology and Technology ◽

10.1590/1678-4324-2017160352 ◽

2017 ◽

Vol 60 (0) ◽

Cited By ~ 4

Author(s):

Farah Deeba ◽

Tasawar Sultana ◽

Bushra Javaid ◽

Tariq Mahmood ◽

S. M. S. Naqvi

Keyword(s):

Oryza Sativa ◽

Abiotic Stress ◽

Stress Tolerance ◽

Molecular Characterization ◽

Abiotic Stress Tolerance ◽

Myb Protein

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From model to crop: functional characterization of SPL8 in M. truncatula led to genetic improvement of biomass yield and abiotic stress tolerance in alfalfa

Plant Biotechnology Journal ◽

10.1111/pbi.12841 ◽

2017 ◽

Vol 16 (4) ◽

pp. 951-962 ◽

Cited By ~ 20

Author(s):

Jiqing Gou ◽

Smriti Debnath ◽

Liang Sun ◽

Amy Flanagan ◽

Yuhong Tang ◽

...

Keyword(s):

Abiotic Stress ◽

Stress Tolerance ◽

Genetic Improvement ◽

Biomass Yield ◽

Abiotic Stress Tolerance ◽

Functional Characterization

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Genome wide identification of NAC transcription factors and their role in abiotic stress tolerance in Chenopodium quinoa

10.1101/693093 ◽

2019 ◽

Cited By ~ 1

Author(s):

Nouf Owdah Alshareef ◽

Elodie Rey ◽

Holly Khoury ◽

Mark Tester ◽

Sandra M. Schmöckel

Keyword(s):

Transcription Factor ◽

Transcription Factors ◽

Abiotic Stress ◽

Stress Tolerance ◽

Abiotic Stresses ◽

Abiotic Stress Tolerance ◽

Chenopodium Quinoa ◽

Phosphate Starvation ◽

Water Deficiency ◽

Genome Wide

AbstractChenopodium quinoa Willd. (quinoa) is a pseudocereal with high nutritional value and relatively high tolerance to several abiotic stresses, including water deficiency and salt stress, making it a suitable plant for the study of mechanisms of abiotic stress tolerance. NAC (NAM, ATAF and CUC) transcription factors are involved in a range of plant developmental processes and in the response of plants to biotic and abiotic stresses. In the present study, we perform a genome-wide comprehensive analysis of the NAC transcription factor gene family in quinoa. In total, we identified 107 quinoa NAC transcription factor genes, distributed equally between sub-genomes A and B. They are phylogenetically clustered into two major groups and 18 subgroups. Almost 75% of the identified CqNAC genes were duplicated two to seven times and the remaining 25% of the CqNAC genes were found as a single copy. We analysed the transcriptional responses of the identified quinoa NAC TF genes in response to various abiotic stresses. The transcriptomic data revealed 28 stress responsive CqNAC genes, where their expression significantly changed in response to one or more abiotic stresses, including salt, water deficiency, heat and phosphate starvation. Among these stress responsive NACs, some were previously known to be stress responsive in other species, indicating their potentially conserved function in response to abiotic stress across plant species. Six genes were differentially expressed specifically in response to phosphate starvation but not to other stresses, and these genes may play a role in controlling plant responses to phosphate deficiency. These results provide insights into quinoa NACs that could be used in the future for genetic engineering or molecular breeding.

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Genome-wide analysis of Myo-inositol oxygenase gene family in tomato reveals their involvement in ascorbic acid accumulation

10.21203/rs.2.14506/v1 ◽

2019 ◽

Author(s):

Shoaib Munir ◽

Muhammad Ali Mumtaz ◽

John Kojo Ahiakpa ◽

Genzhong Liu ◽

Wei Zheng ◽

...

Keyword(s):

Ascorbic Acid ◽

Abiotic Stress ◽

Gene Family ◽

Stress Tolerance ◽

Abiotic Stress Tolerance ◽

Light Response ◽

Genome Wide ◽

Genes Encoding ◽

Transgenic Lines ◽

Distribution Mapping

Abstract Background Ascorbic acid (Vitamin C, AsA) is an antioxidant metabolite involved in plant development and environmental stimuli. AsA biosynthesis has been well studied in plants and MIOX is a critical enzyme in plants AsA biosynthesis pathway. However, myo-inositol oxygenase (MIOX) gene family members and their involvement in AsA biosynthesis and response to abiotic stress remain unclear. Results In this study, five tomato genes encoding MIOX proteins and possessing MIOX motifs were identified. Structural analysis and distribution mapping showed that 5 MIOX genes contain different intron/exon patterns and unevenly distributed among four chromosomes. In addition, expression analyses indicated the remarkable expression of SlMIOX genes in different plant tissues. Furthermore, transgenic lines were obtained by over-expression of MIOX4 gene in tomato. The overexpression lines showed a significant increase in total ascorbate in leaves and red fruits compared to control. Expression analysis revealed that increased accumulation of AsA in MIOX4 overexpression lines is possible as a consequence of the multiple genes involved in AsA biosynthesis. Myo inositol (MI) feeding in leaf and fruit implied that AsA biosynthesis was mainly improved by Myo-inositol pathway in leaves and fruits. MIOX4 overexpression lines exhibited a betterlight response, abiotic stress tolerance, and AsA biosynthesis capacity. Conclusions These results showed that MIOX4 transgenic lines contribute to AsA biosynthesis, evident as better light response and improved oxidative stress tolerance. This study provides the first comprehensive analysis of MIOX gene family and their involvement in ascorbate biosynthesis in tomato.

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