scholarly journals Can Inoculation With the Bacterial Biostimulant Enterobacter sp. Strain 15S Be an Approach for the Smarter P Fertilization of Maize and Cucumber Plants?

2021 ◽  
Vol 12 ◽  
Author(s):  
Mónica Yorlady Alzate Zuluaga ◽  
André Luiz Martinez de Oliveira ◽  
Fabio Valentinuzzi ◽  
Raphael Tiziani ◽  
Youry Pii ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root System ◽  
Nutrient Status ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Inoculation ◽  
P Nutrition ◽  
Promising Alternative ◽  
P Deficiency ◽  
Remarkable Effect ◽  
Maize Plants

Phosphorus (P) is an essential nutrient for plants. The use of plant growth-promoting bacteria (PGPB) may also improve plant development and enhance nutrient availability, thus providing a promising alternative or supplement to chemical fertilizers. This study aimed to evaluate the effectiveness of Enterobacter sp. strain 15S in improving the growth and P acquisition of maize (monocot) and cucumber (dicot) plants under P-deficient hydroponic conditions, either by itself or by solubilizing an external source of inorganic phosphate (Pi) [Ca3(PO4)2]. The inoculation with Enterobacter 15S elicited different effects on the root architecture and biomass of cucumber and maize depending on the P supply. Under sufficient P, the bacterium induced a positive effect on the whole root system architecture of both plants. However, under P deficiency, the bacterium in combination with Ca3(PO4)2 induced a more remarkable effect on cucumber, while the bacterium alone was better in improving the root system of maize compared to non-inoculated plants. In P-deficient plants, bacterial inoculation also led to a chlorophyll content [soil-plant analysis development (SPAD) index] like that in P-sufficient plants (p < 0.05). Regarding P nutrition, the ionomic analysis indicated that inoculation with Enterobacter 15S increased the allocation of P in roots (+31%) and shoots (+53%) of cucumber plants grown in a P-free nutrient solution (NS) supplemented with the external insoluble phosphate, whereas maize plants inoculated with the bacterium alone showed a higher content of P only in roots (36%) but not in shoots. Furthermore, in P-deficient cucumber plants, all Pi transporter genes (CsPT1.3, CsPT1.4, CsPT1.9, and Cucsa383630.1) were upregulated by the bacterium inoculation, whereas, in P-deficient maize plants, the expression of ZmPT1 and ZmPT5 was downregulated by the bacterial inoculation. Taken together, these results suggest that, in its interaction with P-deficient cucumber plants, Enterobacter strain 15S might have solubilized the Ca3(PO4)2 to help the plants overcome P deficiency, while the association of maize plants with the bacterium might have triggered a different mechanism affecting plant metabolism. Thus, the mechanisms by which Enterobacter 15S improves plant growth and P nutrition are dependent on crop and nutrient status.

scholarly journals Forage Mass, Tillering, Nutritive Value and Root System of Ruzigrass Inoculated with Plant Growth Promoting Bacteria Associated with Doses of N-Fertilizer

2020 ◽  
Vol 8 (10) ◽  
pp. 41-55
Author(s):  
Artur Roque Domingues Barreiros ◽  
Ulysses Cecato ◽  
Camila Fernandes Domingues Duarte ◽  
Mariangela Hungria ◽  
Thiago Trento Biserra ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root System ◽  
Nutritive Value ◽  
Block Design ◽  
N Fertilizer ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting

The aim of this study was evaluating the effect of the inoculation of plant growth promoting bacteria (PGPB) in forage mass, tillering, nutritive value and root system of ruzigrass (Urochloa ruziziensis (R. Germ. & Evrard) Crins (syn. of Brachiaria ruziziensis) associated with doses of N-fertilizer. The bacteria inoculated were Azospirillum brasilense Ab-V5, Pseudomonas fluorescens CCTB03 and Pantoea ananatis AMG 521, plus the control treatment (non-inoculated), associated with doses of N-fertilizer (0, 50 and 100 kg N ha-1). The experiment was performed in a randomized block design, in a 4x3 factorial scheme, with four replicates, totaling 48 plots (12 m2). There were no effects of the PGPB and the use of N-fertilizer on the leaf blade, stem+sheath, forage mass, daily and yearly accumulation of forage mass. The PGPB did not have influence on the density of tillers. The doses of 50 and 100 kg of N ha-1 increased the amount of tillers. The AMG 521 strain associated with N-fertilizer provided heavier tillers. There was no effect of the PGPB on crude protein (CP), neutral detergent fiber (NDF), as well as acid detergent fiber (ADF), and in vitro digestibility of the dry matter (IVDDM).  The use of 100 kg of N ha-1 contributed to an increase in CP and a decrease in NDF. The AMG 521 strain contributed to a smaller diameter of the root. Strains CCTB03 and AMG 521 demonstrated a smaller area, length and root density when associated with the dose of 50kg of N ha-1. In general, the PGPB were not efficient in promoting productive increments in ruzigrass.

scholarly journals ROOT SYSTEM IN MAIZE PLANTS CULTIVATED UNDER WATER DEFICIT AND APPLICATION OF CHITOSAN

2020 ◽  
Vol 19 ◽  
pp. 11
Author(s):  
LORENA GABRIELA ALMEIDA ◽  
EDER MARCOS DA SILVA ◽  
PAULO CÉSAR MAGALHÃES ◽  
DÉCIO KARAM ◽  
CAROLINE OLIVEIRA DOS REIS ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root System ◽  
Water Deficit ◽  
Water Availability ◽  
Agricultural Production ◽  
Negative Effects ◽  
Flowering Stage ◽  
Specific Behavior ◽  
Maize Plants ◽  
Corn Plants

Low water availability is characterized as an abiotic stressthat limits the agricultural production. Due to the physical and chemicalcharacteristics of the chitosan (CHT), this substance might stimulatephysiological responses on plants to tolerate the water deficit. In this sense,we submitted corn plants to water deficit and application of chitosan on theleaves (140 mg/L) during pre flowering stage. It were analyzed two cornhybrids genotypes contrasting for water deficit tolerance: DKB 390 (tolerant)and BRS1010 (sensitive). Then, we performed evaluations on the rootsystem and production components. Corn plants submitted to the applicationof chitosan presented a specific behavior: when compared the hybrids,the tolerant one presented a root system that was more developed and anexpressive agronomical yield. These results highlight the fact that the chitosanstimulates plant growth, enhancing their root system and contributing toincrease the availability and absorption of water and nutrients. The chitosanpresents a potential to reduce the negative effects of water deficit on the rootsystems, without compromising the agronomical yield.

scholarly journals Growth response of wheat cultivars to bacterial inoculation in calcareous soil

2010 ◽  
Vol 56 (No. 12) ◽  
pp. 570-573 ◽  
Author(s):  
D. Egamberdieva
Keyword(s):  
Plant Growth ◽  
Calcareous Soil ◽  
Dry Weight ◽  
Plant Growth Promoting Bacteria ◽  
Wheat Cultivars ◽  
Bacterial Strains ◽  
Bacterial Inoculation ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Selection Of

In this study the plant growth-promoting bacteria were analysed for their growth-stimulating effects on two wheat cultivars. The investigations were carried out in pot experiments using calcareous soil. The results showed that bacterial strains Pseudomonas spp. NUU1 and P. fluorescens NUU2 were able to colonize the rhizosphere of both wheat cultivars. Their plant growth-stimulating abilities were affected by wheat cultivars. The bacterial strains Pseudomonas sp. NUU1 and P. fluorescens NUU2 significantly stimulated the shoot and root length and dry weight of wheat cv. Turon, whereas cv. Residence was less affected by bacterial inoculation. The results of our study suggest that inoculation of wheat with Pseudomonas strains can improve plant growth in calcareous soil and it depends upon wheat cultivars. Prior to a selection of good bacterial inoculants, it is recommended to select cultivars that benefit from association with these bacteria.

scholarly journals Diversity and capacity to promote maize growth of bacteria isolated from the Amazon region

2016 ◽  
Vol 46 (2) ◽  
pp. 111-118 ◽  
Author(s):  
Krisle da SILVA ◽  
Liamara PERIN ◽  
Maria de Lourdes GOMES ◽  
Alexandre Cardoso BARAÚNA ◽  
Gilmara Maria Duarte PEREIRA ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Nifh Gene ◽  
Amazon Region ◽  
Forest Area ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Maize Plants ◽  
Growth Promoting

ABSTRACT Maize plants can establish beneficial associations with plant growth-promoting bacteria. However, few studies have been conducted on the characterization and inoculation of these bacteria in the Amazon region. This study aimed to characterize endophytic bacteria isolated from maize in the Amazon region and to assess their capacity to promote plant growth. Fifty-five bacterial isolates were obtained from maize grown in two types of ecosystems, i.e., a cerrado (savanna) and a forest area. The isolates were characterized by the presence of the nifH gene, their ability to synthesize indole-3-acetic acid (IAA) and solubilize calcium phosphate (CaHPO4), and 16S rRNA partial gene sequencing. Twenty-four bacteria contained the nifH gene, of which seven were isolated from maize plants cultivated in a cerrado area and seventeen from a forest area. Fourteen samples showed the capacity to synthesize IAA and only four solubilized calcium phosphate. The following genera were found among these isolates: Pseudomonas; Acinetobacter; Enterobacter; Pantoea; Burkholderia and Bacillus. In addition, eight isolates with plant growth-promoting capacity were selected for a glasshouse experiment involving the inoculation of two maize genotypes (a hybrid and a variety) grown in pots containing soil. Inoculation promoted the development of the maize plants but no significant interaction between maize cultivar and bacterial inoculation was found. A high diversity of endophytic bacteria is present in the Amazon region and these bacteria have potential to promote the development of maize plants.

scholarly journals Plant Growth-Promoting Bacteria as an Emerging Tool to Manage Bacterial Rice Pathogens

2021 ◽  
Vol 9 (4) ◽  
pp. 682
Author(s):  
Mohamad Syazwan Ngalimat ◽  
Erneeza Mohd Hata ◽  
Dzarifah Zulperi ◽  
Siti Izera Ismail ◽  
Mohd Razi Ismail ◽  
...  
Keyword(s):  
Plant Growth ◽  
Large Scale ◽  
Plant Growth Promoting Bacteria ◽  
Promising Alternative ◽  
Rice Plants ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Rice Pathogens ◽  
Grain Losses

As a major food crop, rice (Oryza sativa) is produced and consumed by nearly 90% of the population in Asia with less than 9% produced outside Asia. Hence, reports on large scale grain losses were alarming and resulted in a heightened awareness on the importance of rice plants’ health and increased interest against phytopathogens in rice. To serve this interest, this review will provide a summary on bacterial rice pathogens, which can potentially be controlled by plant growth-promoting bacteria (PGPB). Additionally, this review highlights PGPB-mediated functional traits, including biocontrol of bacterial rice pathogens and enhancement of rice plant’s growth. Currently, a plethora of recent studies address the use of PGPB to combat bacterial rice pathogens in an attempt to replace existing methods of chemical fertilizers and pesticides that often lead to environmental pollutions. As a tool to combat bacterial rice pathogens, PGPB presented itself as a promising alternative in improving rice plants’ health and simultaneously controlling bacterial rice pathogens in vitro and in the field/greenhouse studies. PGPB, such as Bacillus, Pseudomonas, Enterobacter, Streptomyces, are now very well-known. Applications of PGPB as bioformulations are found to be effective in improving rice productivity and provide an eco-friendly alternative to agroecosystems.

ROOT SYSTEM IN MAIZE PLANTS CULTIVATED UNDER WATER DEFICIT AND APPLICATION OF CHITOSAN

2020 ◽  
Vol 19 ◽  
pp. 11
Author(s):  
LORENA GABRIELA ALMEIDA ◽  
EDER MARCOS DA SILVA ◽  
PAULO CÉSAR MAGALHÃES ◽  
DÉCIO KARAM ◽  
CAROLINE OLIVEIRA DOS REIS ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root System ◽  
Water Deficit ◽  
Water Availability ◽  
Agricultural Production ◽  
Negative Effects ◽  
Flowering Stage ◽  
Specific Behavior ◽  
Maize Plants ◽  
Corn Plants

Low water availability is characterized as an abiotic stressthat limits the agricultural production. Due to the physical and chemicalcharacteristics of the chitosan (CHT), this substance might stimulatephysiological responses on plants to tolerate the water deficit. In this sense,we submitted corn plants to water deficit and application of chitosan on theleaves (140 mg/L) during pre flowering stage. It were analyzed two cornhybrids genotypes contrasting for water deficit tolerance: DKB 390 (tolerant)and BRS1010 (sensitive). Then, we performed evaluations on the rootsystem and production components. Corn plants submitted to the applicationof chitosan presented a specific behavior: when compared the hybrids,the tolerant one presented a root system that was more developed and anexpressive agronomical yield. These results highlight the fact that the chitosanstimulates plant growth, enhancing their root system and contributing toincrease the availability and absorption of water and nutrients. The chitosanpresents a potential to reduce the negative effects of water deficit on the rootsystems, without compromising the agronomical yield.

scholarly journals Transcriptional activities of methanogens and methanotrophs vary with methane emission flux in rice soils under chronic nutrient constraints of phosphorus and potassium

2016 ◽  
Vol 13 (23) ◽  
pp. 6507-6518 ◽  
Author(s):  
Rong Sheng ◽  
Anlei Chen ◽  
Miaomiao Zhang ◽  
Andrew S. Whiteley ◽  
Deepak Kumaresan ◽  
...  
Keyword(s):  
Plant Growth ◽  
Nutrient Deficiency ◽  
Chemical Properties ◽  
Experimental System ◽  
Nutrient Status ◽  
Transcript Abundance ◽  
Available Phosphorus ◽  
Community Structures ◽  
Rice Soils ◽  
P Deficiency

Abstract. Nutrient status in soil is crucial for the growth and development of plants which indirectly or directly affect the ecophysiological functions of resident soil microorganisms. Soil methanogens and methanotrophs can be affected by soil nutrient availabilities and plant growth, which in turn modulate methane (CH4) emissions. Here, we assessed whether deficits in soil-available phosphorus (P) and potassium (K) modulated the activities of methanogens and methanotrophs in a long-term (20 year) experimental system involving limitation in either one or both nutrients. Results showed that a large amount of CH4 was emitted from paddy soil at rice tillering stage (flooding) while CH4 flux was minimum at ripening stage (drying). Compared to soils amended with NPK fertiliser treatment, the soils without P input significantly reduced methane flux rates, whereas those without K input did not. Under P limitation, methanotroph transcript copy number significantly increased in tandem with a decrease in methanogen transcript abundance, suggesting that P-deficiency-induced changes in soil physio-chemical properties, in tandem with rice plant growth, might constrain the activity of methanogens, whereas the methanotrophs might be adaptive to this soil environment. In contrast, lower transcript abundance of both methanogen and methanotrophs were observed in K-deficient soils. Assessments of community structures based upon transcripts indicated that soils deficient in P induced greater shifts in the active methanotrophic community than K-deficient soils, while similar community structures of active methanogens were observed in both treatments. These results suggested that the population dynamics of methanogens and methanotrophs could vary along with the changes in plant growth states and soil properties induced by nutrient deficiency.

scholarly journals Plant growth-promoting bacteria associated with nitrogen fertilization at topdressing in popcorn agronomic performance

Bragantia ◽  
2016 ◽  
Vol 75 (1) ◽  
pp. 33-40 ◽  
Author(s):  
Leandro Teodoski Spolaor ◽  
Leandro Simões Azeredo Gonçalves ◽  
Odair José Andrade Pais dos Santos ◽  
André Luiz Martinez de Oliveira ◽  
Carlos Alberto Scapim ◽  
...  
Keyword(s):  
Plant Growth ◽  
Grain Yield ◽  
Nitrogen Fertilization ◽  
Block Design ◽  
N Fertilization ◽  
Plant Growth Promoting Bacteria ◽  
Promising Alternative ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
N Rates

ABSTRACT The use of plant growth-promoting bacteria is a promising alternative with low environmental impact to increase the efficiency of use of chemical fertilizers, ensuring high yield with better cost-effective ratio. In maize crops, several studies have demonstrated an increased yield when Azospirillum-based inoculants are used. In the case of popcorn, there are no available studies related to use of inoculation and its response on yield parameters. Thus, the aim of this study was to evaluate the field performance of popcorn when inoculated with the commercial product Masterfix L (A. brasilense Ab-V5 and A. brasilense Ab-V6) and the non-commercial inoculant UEL (A. brasilense Ab-V5 + Rhizobium sp. 53GRM1) associated with nitrogen fertilization. The trials were conducted in Londrina and Maringá, Paraná State, Brazil, in a randomized block design with four replications, in a split plot design with the inoculation treatments located in the plots (uninoculated, Masterfix L, and UEL) and the different N rates located in the subplots where ammonium sulphate was applied in the topdressing at the V6 stage (0, 50, 100, and 150 kg∙ha–1). The variance analysis showed significant effects (p < 0.05) of inoculation (Londrina environment) and N rates (both environments) only for grain yield. There was no inoculation effect in the grain yield when inoculants were applied together with N-fertilization at topdressing. In the absence of N-fertilization at topdressing, the inoculants Masterfix L. and UEL promoted higher grain yield as compared to the uninoculated plants, with resulting increases of 13.21 and 26.61% in yield, respectively.

scholarly journals Enhancing the Rice Seedlings Growth Promotion Abilities of Azoarcus sp. CIB by Heterologous Expression of ACC Deaminase to Improve Performance of Plants Exposed to Cadmium Stress

2020 ◽  
Vol 8 (9) ◽  
pp. 1453 ◽  
Author(s):  
Helga Fernández-Llamosas ◽  
Juan Ibero ◽  
Sofie Thijs ◽  
Valeria Imperato ◽  
Jaco Vangronsveld ◽  
...  
Keyword(s):  
Plant Growth ◽  
Ethylene Production ◽  
Acc Deaminase ◽  
Cloning And Expression ◽  
Plant Production ◽  
Broad Host Range ◽  
Plant Growth Promoting Bacteria ◽  
Rice Seedlings ◽  
Plant Weight ◽  
Promising Alternative

Environmental pollutants can generate stress in plants causing increased ethylene production that leads to the inhibition of plant growth. Ethylene production by the stressed plant may be lowered by Plant Growth-Promoting Bacteria (PGPB) that metabolizes the immediate precursor of ethylene 1-aminocyclopropane-1-carboxylate (ACC). Thus, engineering PGPB with ACC deaminase activity can be a promising alternative to mitigate the harmful effects of pollutants and thus enhance plant production. Here we show that the aromatics-degrading and metal-resistant Azoarcus sp. CIB behaves as a PGP-bacterium when colonizing rice as an endophyte, showing a 30% increment in plant weight compared to non-inoculated plants. The cloning and expression of an acdS gene led to a recombinant strain, Azoarcus sp. CIB (pSEVA237acdS), possessing significant ACC deaminase activity (6716 nmol mg−1 h−1), constituting the first PGPB of the Rhodocyclaceae family equipped with this PGP trait. The recombinant CIB strain acquired the ability to protect inoculated rice plants from the stress induced by cadmium (Cd) exposure and to increase the Cd concentration in rice seedlings. The observed decrease of the levels of reactive oxygen species levels in rice roots confirms such a protective effect. The broad-host-range pSEVA237acdS plasmid paves the way to engineer PGPB with ACC deaminase activity to improve the growth of plants that might face stress conditions.

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