scholarly journals Can Spectrophotometry Be Used to Quantify Zingiberene Sesquiterpenoids in Tomato Leaflet Extracts?

Agriculture ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1037
Author(s):  
Mohammad Dawood ◽  
John C. Snyder
Keyword(s):  
Interspecific Hybrid ◽  
Quantitative Estimate ◽  
Quantitative Method ◽  
Uv Light ◽  
Uv Absorbance ◽  
Double Bonds ◽  
Multiple Wavelengths ◽  
The Relationship ◽  
Absorbance Spectra

The presence of 7-epi zingiberene in wild tomatoes has been associated with arthropod resistance. Consequently, tomato breeders are attempting to introgress 7-epi zingiberene from wild to cultivated tomato requiring quantification of zingiberene. 7-Epi zingiberene likely absorbs UV light due to its conjugated double bonds and others have claimed that measurement of absorbance at 270 nm of tomato leaflet washes can be used to quantify zingiberene. However, this claim has never been critically evaluated. We initially evaluated this claim in an interspecific hybrid tomato generation that was segregating widely for zingiberene. Results indicated that the method does not obey the Beer–Lambert law. Consequently, we examined in detail aspects of the UV-absorbance of isolated zingiberenoids and leaflet washes obtained from parents and interspecific generations that were segregating for 7-epi zingiberene. Results indicated that isolated zingiberenoids, as well as leaflet washes containing zingiberenoids, have broad absorbance spectra with a λmax of 264 nm. For isolated zingiberenoids, the relationship between abundance and absorbance at 264 nm did obey the Beer–Lambert law. Average absorbance spectra for leaflet washes from interspecific generation plants showed subtle λmax shifts. Furthermore, the relationship between absorbance at 264 nm and zingiberenoid content of these generations did not obey the Beer–Lambert law. The use of multiple wavelengths for estimation of zingiberenoids in these breeding generations was explored and the inclusion of additional absorbances at one or two wavelengths always improved estimates. However, identified wavelength(s) differed among generations. Taken together, the results indicate that measurement of absorbance of tomato leaflet washes at a single wavelength is not a reliable quantitative estimate of zingiberenoids in leaflet washes. Estimates can be improved by utilizing absorbance at multiple wavelengths, but the particular wavelengths will vary among generations. Lastly, measurement of absorbance may be useful for identifying those relatively rare individuals in a generation that is widely segregating for zingiberenoid content. However, even in this situation, the determination of the actual 7-epi zingiberene content would need to be backstopped by a valid quantitative method.

scholarly journals Can Spectrophotometry Be Used to Quantify Zingiberene Sesquiterpenoids in Tomato Leaflet Extracts?

2021 ◽  
Author(s):  
Mohammad Dawood ◽  
John C. C Snyder
Keyword(s):  
Uv Light ◽  
Spider Mites ◽  
Wild Relative ◽  
Vegetable Crop ◽  
Improve Accuracy ◽  
Multiple Wavelengths ◽  
Arthropod Pests ◽  
The Relationship ◽  
Absorbance Spectra ◽  
Uv Range

Tomato (Solanum lycopersicum), an important vegetable crop, is a host for numerous pests and pathogens. Consequently, tomato breeders are striving to improve pest resistance. Many accessions of the wild relative S. habrochaites display high resistance towards arthropod pests such as spider mites and whiteflies and the presence of the sesquiterpene hydrocarbon 7-epi-zingiberene on its trichomes has been associated with arthropod resistance. Consequently, tomato breeders are attempting to introgress 7-epi-zingiberene from wild to cultivated tomato. 7-epi-zingiberene has conjugated double bonds causing it to absorb UV light. In fact, absorbance at 270 nm of tomato leaflet washes has been used to aid introgression, but its use has not been critically evaluated. Consequently, we evaluated the relationships between UV absorbances in the 200-350 nm range and the concentration of 7-epi-zingiberene in plants from several interspecific hybrid generations of tomato. We also evaluated the absorbance spectra for isolated 7-epizingberene and it two oxidized forms. The wavelength of maximum absorbance of the isolated compounds was 264 nm. The strength of the relationship (R²) between absorbance measured at a single wavelength and quantity zingiberene ranged from 0.30 to 0.92 among generations evaluated. Additionally, the regressions of absorbance at a single wavelength on quantity always resulted in a non-zero intercept, indicating that single wavelength absorbance cannot be directly used for quantitation. Use of multiple wavelengths for quantitation was explored and their use can improve accuracy of quantitation. Measuring absorbance at multiple wavelengths or scanning in the UV range, backstopped by a method of quantitation such as gas chromatography could be a useful tool for aiding introgression.

Quantitative Assessment of Hyoid Bone Displacement From Video Images During Swallowing

1995 ◽  
Vol 38 (3) ◽  
pp. 579-585 ◽  
Author(s):  
Adrienne L. Perlman ◽  
Douglas J. VanDaele ◽  
Melissa S. Otterbacher
Keyword(s):  
Quantitative Method ◽  
Quantitative Assessment ◽  
Hyoid Bone ◽  
Video Images ◽  
Hyoid Movement ◽  
The Relationship ◽  
Male Subjects

Given the relationship between hyoid displacement and aspiration, a simple, accurate, clinically useful method for identification of reduced hyoid displacement is needed. The purpose of this investigation was to validate a quantitative method for clinically assessing hyoid bone movement during swallowing. Videofluoroscopic evaluations of 20 male subjects—10 who had been subjectively assessed as demonstrating normal hyoid displacement during swallowing, and 10 who had been subjectively assessed as demonstrating reduced displacement during swallowing—were analyzed. A video measuring gauge was used, and a software spreadsheet was developed to perform trigonometric calculations for determination of anterior/ superior hyoid trajectories. Statistically significant differences were found between groups in both the anterior and superior directions of hyoid movement. No significant differences were found between liquid and paste swallows within groups. This method is simple in that it is necessary to identify only three to five points on two video images. Those values are then input onto a preprogrammed spreadsheet that automatically performs the necessary calculations. Although the present investigation focused on the movements of the hyoid bone during swallowing, this method of tracking displacement can be used for virtually any task or structure whose movements can be captured on videotape.

Thromboplastin Related Differences in the Determination of International Normalised Ratio: A Cause for Concern?

1994 ◽  
Vol 72 (03) ◽  
pp. 426-429 ◽  
Author(s):  
S Kitchen ◽  
I D Walker ◽  
T A L Woods ◽  
F E Preston
Keyword(s):  
Patient Management ◽  
External Quality Assessment ◽  
Oral Anticoagulant Therapy ◽  
Warfarin Dosage ◽  
External Quality ◽  
External Quality Assessment Scheme ◽  
International Normalised Ratio ◽  
The Uk ◽  
The Relationship

SummaryWhen the International Normalised Ratio (INR) is used for control of oral anticoagulant therapy the same result should be obtained irrespective of the laboratory reagent used. However, in the UK National External Quality Assessment Scheme (NEQAS) for Blood Coagulation INRs determined using different reagents have been significantly different.For 18 NEQAS samples Manchester Reagent (MR) was associated with significantly lower INRs than those obtained using Diagen Activated (DA, p = 0.0004) or Instrumentation Laboratory PT-Fib HS (IL, p = 0.0001). Mean INRs for this group were 3.15, 3.61, and 3.65 for MR, DA, and IL respectively. For 61 fresh samples from warfarin-ised patients with INRs of greater than 3.0 the relationship between thromboplastins in respect of INR was similar to that observed for NEQAS data. Thus INRs obtained with MR were significantly lower than with DA or IL (p <0.0001). Mean INRs for this group were 4.01, 4.40, and 4.59 for MR, DA, and IL respectively.We conclude that the differences between INRs measured with the thromboplastins studied here are sufficiently great to influence patient management through warfarin dosage schedules, particularly in the upper therapeutic range of INR. There is clearly a need to address the issues responsible for the observed discrepancies.

Modern determinants of endometrium hyperplastic processes pathogenesis

2016 ◽  
pp. 137-142
Author(s):  
V.O. Benyuk ◽  
◽  
V.M. Goncharenko ◽  
T.R. Nykoniuk ◽  
◽  
...  
Keyword(s):  
Conservative Therapy ◽  
Solid Phase ◽  
Research Direction ◽  
Immunohistochemical Method ◽  
Results Of Treatment ◽  
Treatment And Prevention ◽  
Receptor Phenotype ◽  
The Relationship ◽  
Endometrial Proliferation

The objective: to еxplore the relationship between the activity of endometrial proliferation and the state of the local immune response in the uterus in the conditions berprestasi process. Patients and methods. Examined 228 women of reproductive and perimenopausal age with endometrial pathology using ultrasound and then performing hysteroresectoscopy. Determination of the concentrations of the cytokines IL-1, IL-2, IL-6 and TNF was performed by solid phase ELISA. Results. Found a trend that confirms the loss of sensitivity to hormones at the stage of malignancy of the endometrium and can be used as diagnostic determinants in determining the nature of intrauterine pathology and criterion of the effectiveness of conservative therapy. Conclusion. Improving etiopatogenetice approach to the therapy of hyperplastic proce.sses of endometrium with determination of receptor phenotype of the endometrium is a research direction in modern gynecology, which will help to improve the results of treatment and prevention of intrauterine pathology. Key words: endometrial hyperplasia,the receptors for progesterone and estrogen, immunohistochemical method.

Calculating the aerodynamics of vertical axis wind turbines

2012 ◽  
Vol 34 (3) ◽  
pp. 169-184 ◽  
Author(s):  
Hoang Thi Bich Ngoc
Keyword(s):  
Wind Turbine ◽  
Incidence Angle ◽  
Vertical Axis ◽  
Rotor Blades ◽  
Vertical Axis Wind Turbine ◽  
Horizontal Axis Wind Turbine ◽  
Velocity Triangle ◽  
Relationship Of ◽  
The Relationship

Vertical axis wind turbine technology has been applied last years, very long after horizontal axis wind turbine technology. Aerodynamic problems of vertical axis wind machines are discussible. An important problem is the determination of the incidence law in the interaction between wind and rotor blades. The focus of the work is to establish equations of the incidence depending on the blade azimuth, and to solve them. From these results, aerodynamic torques and power can be calculated. The incidence angle is a parameter of velocity triangle, and both the factors depend not only on the blade azimuth but also on the ratio of rotational speed and horizontal speed. The built computational program allows theoretically selecting the relationship of geometric parameters of wind turbine in accordance with requirements on power, wind speed and installation conditions.

Tire Treadwear — A Comprehensive Evaluation of the Factors: Generic Type, Aspect Ratio, Tread Pattern, and Tread Composition Part I: Introduction and Details on the Organization of the Program

1986 ◽  
Vol 14 (4) ◽  
pp. 201-218 ◽  
Author(s):  
A. G. Veith
Keyword(s):  
Aspect Ratio ◽  
Comprehensive Evaluation ◽  
Test Method ◽  
Interactive Effects ◽  
Data Analyses ◽  
Main Effect ◽  
Systematic Determination ◽  
Relationship Of ◽  
The Relationship

Abstract This four-part series of papers addresses the problem of systematic determination of the influence of several tire factors on tire treadwear. Both the main effect of each factor and some of their interactive effects are included. The program was also structured to evaluate the influence of some external-to-tire conditions on the relationship of tire factors to treadwear. Part I describes the experimental design used to evaluate the effects on treadwear of generic tire type, aspect ratio, tread pattern (groove or void level), type of pattern (straight rib or block), and tread compound. Construction procedures and precautions used to obtain a valid and functional test method are included. Two guiding principles to be used in the data analyses of Parts II and III are discussed. These are the fractional groove and void concept, to characterize tread pattern geometry, and a demonstration of the equivalence of wear rate for identical compounds on whole tread or multi-section tread tires.

The Awareness Level of the Safety and Health of the Operational Level

2017 ◽  
Vol 3 (1) ◽  
pp. 42
Author(s):  
Roshanira Che Mohd Noor ◽  
Nur Atiqah Rochin Demong
Keyword(s):  
Health And Safety ◽  
Health Conditions ◽  
Effective Strategies ◽  
Operational Level ◽  
Safety And Health ◽  
Health Factors ◽  
The Cost ◽  
The Relationship ◽  
Awareness Level

Providing a safe and healthy workplace is one of the most effective strategies in for holding down the cost of doing construction business. It was a part of the overall management system to facilitate themanagement of the occupational health and safety risk that are associated with the business of the organization. Factors affected the awareness level inclusive of safety and health conditions, dangerous working area, long wait care and services and lack of emergency communication werethe contributed factors to the awareness level for the operational level. Total of 122 incidents happened at Telekom Malaysia Berhad as compared to year 2015 only 86 cases. Thus, the main objective of this study was to determine the relationship between safety and health factors and the awareness level among operational workers.The determination of this research was to increase the awareness level among the operational level workerswho committing to safety and health environment.

PROFITABILITY ANALYSIS OF TERUBUK FARMING (SACCHARUM EDULE HASSKARL) IN SUBDISTRICT TOSA DISTRICT OF EAST TIDORE OF TIDORE ISLAND

2018 ◽  
Author(s):  
Suwandi S. Sangadji
Keyword(s):  
Data Collection ◽  
Research Method ◽  
Quantitative Method ◽  
Secondary Data ◽  
Primary Data ◽  
Profitability Analysis ◽  
Sample Method ◽  
Document Review ◽  
Revenue Production

The purpose of this researchment is to ascertain how wide the farming of species Saccharun Edule Hasskarl (terubuk) in sub district Tosa, district of East Tidore of Tidore Island through the indicator of the value revenue, production and selling prices so that the farmers will achieve The Break Event Point (BEP). The research method was used a quantitative method with the number of samples of 30 people. The determination of the sample method is using the census method or involving all members of the population into a sample of researchment. The secondary data collection was done by using library literature in the form of document review and relevant references to research object while primary data collection was done by using questionnaire. The data is using equation R /C Ratio, BEP Revenue, BEP Price, and BEP Production. Therefore from the results of the researchment it can be explained that the two of the thirty farmers come through the break event point, while the other twenty-eight farmers declared having a business that worth to be develop or experiencing profit, because the R/C ratio is above 1.0 with average profit reach Rp. 989.000, - per production / farmer.

DETERMINATION OF P53 GENE CODON 72 POLYMORPHISMS IN PATIENTS WITH GASTRIC CANCER

2013 ◽  
pp. 11-17
Author(s):  
Thi Tuy Ha Nguyen ◽  
Thi Minh Thi Ha
Keyword(s):  
Gastric Cancer ◽  
Cancer Patients ◽  
P53 Gene ◽  
Diffuse Gastric Cancer ◽  
Codon 72 ◽  
Cancer Group ◽  
Pcr Rflp ◽  
Gastric Cancer Patients ◽  
The Relationship

Background: The role of p53 gene in the gastric cancer is still controversial. This study is aimed at determining the rate of the p53 gene codon 72 polymorphisms in gastric cancer patients and evaluating the relationship between these polymorphisms and endoscopic and histopathological features of gastric cancer. Patients and methods: Sixty eight patients with gastric cancer (cases) and one hundred and thirty six patients without gastric cancer (controls) were enrolled. p53 gene codon 72 polymorphisms were determined by PCR-RFLP technique with DNA extracted from samples of gastric tissue. Results: In the group of gastric cancer, Arginine/Argnine, Arginine/Proline and Proline/Proline genotypes were found in 29.4%, 42.7% and 27.9%, respectively. The differences of rates were not statistically significant between cases and controls (p > 0,05). In males, the Proline/Proline genotype was found in 38.1% in patients with gastric cancer and more frequent in patients without gastric cancer (15.7%, p = 0,01). An analysis of ROC curve showed that the cut-off was the age of 52 in the Proline/Proline genotype, but it was 65 years old in the Arginine/Proline genotype. The Proline/Proline genotype was found in 41.9% in Borrmann III/IV gastric cancer, this rate was higher than Borrmann I/II gastric cancer (16.2%, p = 0.037) and also higher than controls (18.4%, p = 0,01). The rate of Proline/Proline genotype was 41.7% in the diffuse gastric cancer, it was higher than in controls (p = 0,023). Conclusion: No significative difference of rate was found in genotypes between gastric cancer group and controls. However, there was the relationship between Proline/Proline genotype and gastric cancer in males, Borrmann types of gastric cancer, the diffuse gastric cancer. Key words: polymorphism, codon 72, p53 gene, PCR - RFLP, gastric cancer.

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