scholarly journals Effect of Various Strains of Lactobacillus buchneri on the Fermentation Quality and Aerobic Stability of Corn Silage

Agriculture ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 95
Author(s):  
Mariele Nascimento Agarussi ◽  
Odilon Pereira ◽  
Leandro da Silva ◽  
Vanessa da Silva ◽  
Rosinea de Paula ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Production Systems ◽  
Corn Silage ◽  
Negative Consequences ◽  
Acetic Acid Production ◽  
Lactobacillus Buchneri ◽  
Randomized Design ◽  
Aerobic Stability ◽  
Wild Strains ◽  
Fermentation Profile

The aerobic deterioration of silage nutrients is inevitable in tropical countries, causing negative consequences in animal production systems. Aiming to minimize the losses, the effects of Lactobacillus buchneri strains on fermentation profile and aerobic stability of corn silages were evaluated. The experiment was conducted under a completely randomized design with 13 treatments and three replicates. The treatments were noninoculated, commercial L. buchneri (CI), and 11 wild strains of L. buchneri: LB-56.1, LB-56.2, LB-56.4, LB-56.7, LB-56.8, LB-56.9, LB-56.21, LB-56.22, LB-56.25, LB-56.26, and LB-56.27. The treatments could be divided into three different groups according to silage pH and acetic acid concentration. Silages inoculated with LB-56.1, LB-56.4, and LB-56.9 presented higher pH, whereas intermediate values were observed for LB-56.2, LB-56.7, and LB-56.8. The highest acetic acid production was observed for LB-56.1 and LB-56.7. On the other hand, lowest concentrations were found for CI, LB-56.22, LB-56.25, LB-56.26, and LB-56.27. Higher amounts of NH3–N were observed for LB-56.8, LB-56.21, LB-56.22, and LB-56.27 silages than others. Silage inoculation with CI, LB-56.1, LB-56.2, LB-56.4, LB-56.8, LB-56.9, and LB-56.25 strains had higher aerobic stability than others (59.7 vs. 41.2 h). The L. buchneri strains LB-56.1, LB-56.2, LB-56.4, LB-56.8, LB-56.9, and LB-56.25 provided potential features to improve the aerobic stability of corn silage.

scholarly journals Screening of High 1,2-Propanediol Production by Lactobacillus buchneri Strains and Their Effects on Fermentation Characteristics and Aerobic Stability of Whole-Plant Corn Silage

Agriculture ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 590
Author(s):  
Zhipeng Huang ◽  
Musen Wang ◽  
Wencan Ke ◽  
Xusheng Guo
Keyword(s):  
Acetic Acid ◽  
Dry Matter ◽  
Reference Strain ◽  
Corn Silage ◽  
Acetic Acid Production ◽  
Lactobacillus Buchneri ◽  
Aerobic Stability ◽  
Whole Plant ◽  
Fermentation Quality ◽  
Alfalfa Silage

The study was conducted to screen high 1,2-propanediol produced by Lactobacillus buchneri strains, isolated from baled silages stored for 1 or 2 years, and to evaluate their effects on fermentation quality and aerobic stability of whole-plant corn silage. In total, 31 L. buchneri strains were isolated from alfalfa, whole-plant corn and oat silages. Based on growth performance and 1,2-propanediol and acetic acid production, two strains, L. buchneri 9-2 and L. buchneri 10-1, from alfalfa silage, were further assessed in an ensiling trial on whole-plant corn. The corn silage inoculated with L. buchneri 9-2 or L. buchneri 10-1 had a higher concentration of 1,2-propanediol (34.7 or 34.6 g/kg dry matter (DM)) and acetic acid (47.2 or 45.9 g/kg DM) in comparison with L. buchneri 40788 (reference strain) treated silage (19.5 and 35.9 g/kg DM) after 90 d of fermentation. In addition, these two strains performed better in improving silage aerobic stability relative to control and L. buchneri 40788. The results above indicated that L. buchneri 9-2 and L. buchneri 10-1 could be candidate strains to increase 1,2-propanediol and acetic acid concentrations and improve the aerobic stability of whole-plant corn silage.

Do re-ensiling time and application of Lactobacillus buchneri alter the characteristics of sugarcane silage?

2020 ◽  
Vol 158 (5) ◽  
pp. 438-446
Author(s):  
E. F. S. Faria ◽  
T. C. da Silva ◽  
D. dos S. Pina ◽  
E. M. Santos ◽  
M. L. G. M. L. de Araújo ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Dry Matter ◽  
Repeated Measure ◽  
Repeated Measure Design ◽  
Air Exposure ◽  
Lactobacillus Buchneri ◽  
Aerobic Stability ◽  
Measure Design ◽  
Fermentation Profile ◽  
Set Up

AbstractThis study aimed to examine the effects of re-ensiling time and Lactobacillus buchneri on the fermentation profile, chemical composition and aerobic stability of sugarcane silages. The experiment was set up as a repeated measure design consisting of four air-exposure periods (EP)(0, 6, 12, and 24 h) microbial additive (A) (L. buchneri; or lack of there), with five replicates. Sugarcane was ground through a stationary forage chopper and ensiled in four plastic drums of 200-L capacity. After 210 days of storage, the drums were opened and half of the silage mass was treated with L. buchneri at the concentration of 105 cfu/g of forage. Subsequently, the silages were divided into stacks. The re-ensiling process was started immediately, at 0, 6, 12 and 24-hour intervals, by transferring the material to PVC mini-silos. Silos were opened after 120 days of re-ensiling. The use of L. buchneri reduced butyrate concentration but did not change ethanol or acetic acid concentrations and aerobic stability. An interaction effect between L. buchneri and re-ensiling time was observed for dry matter (DM) losses and composition. Lactobacillus buchneri is not effective in improving aerobic stability in re-ensiled sugarcane silages. However, less DM is lost in silages treated with L. buchneri and exposed to air for 24 h. Re-ensiling sugar cane in up to 24 h of exposure to air does not change final product quality.

scholarly journals Nutritional composition and aerobic stability of wheat and corn silages stored under different environmental conditions

2018 ◽  
Vol 39 (1) ◽  
pp. 253
Author(s):  
Marcos Rogério Oliveira ◽  
Antônio Vinícius Iank Bueno ◽  
Guilherme Fernando Mattos Leão ◽  
Mikael Neumann ◽  
Clóves Cabreira Jobim
Keyword(s):  
Acetic Acid ◽  
Propionic Acid ◽  
Butyric Acid ◽  
Nutritional Quality ◽  
Dry Matter ◽  
Corn Silage ◽  
Transgenic Corn ◽  
Hybrid Corn ◽  
Aerobic Stability ◽  
Fermentation Profile

We aimed to evaluate nutritional quality, fermentation profile, aerobic stability, and dry matter losses in corn (Zea mays) and wheat (Triticum aestivum 'BRS Umbu') silages. Treatments included uninoculated and inoculated (Lactobacillus plantarum and Pediococcus acidilactici, 1.0 × 105 UFC g-1) wheat silage, corn silage from a conventional hybrid and a transgenic hybrid. Nutritional quality and fermentation profile variables were tested in a completely randomized design. Means were compared using Tukey’s test at 5% significance. An aerobic stability trial was conducted in a factorial design with two silages (wheat × inoculated wheat; conventional hybrid corn × transgenic hybrid corn) and two temperatures (ambient temperature × controlled temperature at 24°C). Data were submitted to ANOVA and means were analyzed by the F test at 5% probability. Inoculation of wheat silage increased dry matter, organic matter, and total carbohydrates, but reduced crude protein by a dilution effect. Regarding the fermentation profile, inoculation reduced acetic acid and butyric acid content, whereas it increased propionic acid in wheat silage. Bt corn hybrid silage showed higher dry matter and lower neutral detergent fiber, whereas transgenic corn silage showed lower content of acetic acid, propionic acid, alcohol, and ammonia. Conversely, Bt hybrid silage showed higher butyric acid. Transgenic corn silage showed higher temperature than the conventional hybrid silage during aerobic exposure. Inoculated wheat silage experienced larger deterioration and dry matter losses during the aerobic stability trial. Temperature control worsened aerobic stability in all treatments, increasing dry matter losses and heating.

Effects of xylanase on the fermentation profile and chemical composition of sugarcane silage

2018 ◽  
Vol 156 (9) ◽  
pp. 1123-1129 ◽  
Author(s):  
T. A. Del Valle ◽  
G. Antonio ◽  
T. F. Zenatti ◽  
M. Campana ◽  
E. M. C. Zilio ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Chemical Composition ◽  
Acid Concentration ◽  
Quadratic Effect ◽  
Positive Effects ◽  
Silage Fermentation ◽  
Randomized Design ◽  
Aerobic Stability ◽  
Xylanase Enzyme ◽  
Fermentation Profile

AbstractThe current study aims to evaluate the effects of increasing levels of xylanase enzyme (XYL) on sugarcane silage fermentation, fermentative losses, chemical composition, dry matter (DM), neutral detergent fibre (NDF) degradation and aerobic stability. A completely randomized design trial was performed with five treatments and 50 experimental silos. Treatments were: 0, 100, 200, 300 and 400 mg of XYL per kg of DM. XYL contained 10 000 U/g. There was a quadratic effect of XYL on silage pH and acetic acid concentration: lower pH and higher acetic acid concentrations were found at intermediary levels of the enzyme. XYL decreased lactic acid concentration linearly. Furthermore, the enzyme had a quadratic effect on effluent and total losses, with higher losses at intermediary XYL levels. There was a quadratic effect of XYL on organic matter (OM), non-fibre carbohydrates (NFC) and crude protein (CP) content. In addition, a quadratic effect of XYL was observed on NDF content and degradation. Intermediary levels of XYL showed higher concentration of OM and NFC. The addition of XYL had no effect on silage temperature and pH after aerobic exposure. Thus, intermediate levels of XYL increased acetic acid and decreased silage pH. Besides positive effects on silage composition, intermediary XYL levels decreased NDF degradation.

scholarly journals Effects of Microbial Inoculation and Storage Length on Fermentation Profile and Nutrient Composition of Whole-Plant Sorghum Silage of Different Varieties

2021 ◽  
Vol 12 ◽  
Author(s):  
E. Cole Diepersloot ◽  
Matheus R. Pupo ◽  
Lucas G. Ghizzi ◽  
Jessica O. Gusmão ◽  
Celso Heinzen ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Sweet Sorghum ◽  
Microbial Inoculation ◽  
Randomized Design ◽  
Aerobic Stability ◽  
Fermentation Profile ◽  
Sorghum Sudangrass ◽  
Sorghum Silage ◽  
And Storage

This study aimed to assess the effects of a heterofermentative microbial inoculant and storage length on fermentation profile, aerobic stability, and nutrient composition in whole-plant sorghum silage (WPSS) from different varieties. Experiment 1, a completely randomized design with a 2 × 3 factorial treatment arrangement, evaluated microbial inoculation [CON (50 mL distilled water) or LBLD (Lactobacillus plantarum DSM 21762, L. buchneri DSM 12856, and L. diolivorans DSM 32074; 300,000 CFU/g of fresh forage)] and storage length (14, 28, or 56 d) in forage WPSS. The LBLD silage had lower pH compared to CON, and greater concentrations of succinic acid, ethanol, 1,2-propanediol (1,2-PD), 1-propanol, 2,3-butanediol and total acids. After 56 d, lactic acid concentration was greater for CON, while acetic acid and aerobic stability were greater in LBLD silage. Experiment 2, a completely randomized design with a 2 × 3 factorial treatment arrangement, evaluated effects of microbial inoculation (same as experiment 1) and storage length (14, 28, or 56 d) in WPSS of three varieties [forage sorghum (Mojo Seed, OPAL, Hereford, TX), sorghum-sudangrass (Dyna-gro Seed, Fullgraze II, Loveland, CO, United States), or sweet sorghum (MAFES Foundation Seed Stocks, Dale, MS State, MS)]. The LBLD forage sorghum had greater acetic acid and 1,2-PD concentrations at 56 d and 28 d, respectively, but lower concentrations of propionic acid at 56 d and butyric acid at 14 and 28 d. Additionally, WSC concentration was greater for CON than LBLD at 28 d. Furthermore, CON sweet sorghum had greater lactic acid, propionic acid, and butyric acid concentrations. However, greater acetic acid and 1,2-PD were observed for LBLD sweet sorghum. The CON sweet sorghum had greater concentration of WSC and yeast counts. The CON sorghum sudangrass had greater lactic and butyric acid concentrations than LBLD at 14 d, but lower acetic acid and 1,2-PD concentrations at 56 d. Yeast counts were greater for CON than LBLD sorghum sudangrass silage. Overall, results indicate inoculation of WPSS with Lactobacillus plantarum DSM 21762, L. buchneri DSM 12856, and L. diolivorans DSM 32074 improves heterofermentative co-fermentation allowing the accumulation of acetic acid concentration and increasing antifungal capacities and aerobic stability of WPSS.

scholarly journals A combination of Lactobacillus buchneri and Pediococcus pentosaceus extended the aerobic stability of conventional and BMR-corn hybrids ensiled at low dry matter concentrations by causing a major shift in their bacterial and fungal community

2021 ◽  
Author(s):  
J J Romero ◽  
J Park ◽  
Y Joo ◽  
Y Zhao ◽  
M Killerby ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Relative Abundance ◽  
Dry Matter ◽  
Nutritive Value ◽  
Microbial Populations ◽  
Corn Hybrids ◽  
Pediococcus Pentosaceus ◽  
Lactobacillus Buchneri ◽  
Randomized Design ◽  
Aerobic Stability

Abstract We evaluated the effects of applying a combination inoculant to 4 corn hybrids harvested at high moisture on their nutritive value and microbial populations. The treatment design was the factorial combination of corn hybrids ensiled with (INO) and without (CON) inoculant. The hybrids were TMF2R737 (MCN), F2F817 (MBR), P2089YHR (PCN), and PI144XR (PBR), ensiled at dry matter (DM) concentrations of 30.5, 26.3, 31.1, and 31.5%, respectively; MBR and PBR were brown midrib mutants (BMR). The inoculant contained Lactobacillus buchneri and Pediococcus pentosaceus (4 × 10 5 and 1 × 10 5 cfu/g of fresh corn). The experiment had a complete randomized design with treatments replicated 6 times. Corn was treated or not with inoculant, packed into 7.6L bucket silos, and stored for 100 d. At d 0, the relative abundance (RA, %) of Enterobacteriaceae was lower in PBR vs. the other hybrids [51.3 vs x= (average of) 58.4] and in the case of fungi, incertae sedis (i.s.) Tremellales and Mucoraceae were more and less abundant, respectively, in conventional hybrids vs. BMRs (x= 25.8 vs. x= 13.9 and x= 3.64 vs. x= 7.52; P < 0.04). After ensiling, INO had higher LAB (9.3 vs. 7.1 log cfu/g of fresh corn) and acetic acid (3.44 vs. 1.32% of DM) and lower yeast (3.1 vs. 4.6) and molds (1.5 vs. 3.0), and also extended the aerobic stability (582 vs. 111h) but decreased DM recovery (95.6 vs. 97.4%) vs. CON (P < 0.02). Inoculation reduced bacterial phylogenetic diversity (6.75 vs. 14.4) but increased fungal observed taxonomical units (46 vs. 20) vs. CON (P < 0.01). Also, a higher relative abundance (RA) for Lactobacillaceae (99.2 vs. 75.7%) and lower for Enterobacteriaceae (0.28 vs. 9.93) was observed due to inoculation (P < 0.001). For fungi, INO had a lower RA compared to CON for Monascaceae (12.6 vs. 44.7) and increased i.s. Tremellales (8.0 vs. 1.2) and i.s. Saccharomycetales (6.4 vs. 0.3%; P < 0.006). Inoculation changed the diverse bacterial community found in the phyllosphere across hybrids to a taxonomically uneven one dominated by Lactobacillaceae. In the case of fungi, INO application increased the fungal diversity at d 100 mainly by reducing the dominance of Monascaceae vs. CON. In conclusion, the INO treatment overwhelmed the disparate microbial populations found across BMR and conventional hybrids ensiled at low DM concentrations and ensured a significant concentration of acetic acid that modified fungal populations and in turn extended the aerobic stability of all hybrids.

scholarly journals Effects of a chemical additive on the fermentation, microbial communities, and aerobic stability of corn silage with or without air stress during storage

2020 ◽  
Vol 98 (8) ◽  
Author(s):  
Érica B da Silva ◽  
Rebecca M Savage ◽  
Amy S Biddle ◽  
Stephanie A Polukis ◽  
Megan L Smith ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Storage Period ◽  
Illumina Miseq ◽  
Corn Silage ◽  
Potassium Sorbate ◽  
Chemical Additive ◽  
Pichia Kudriavzevii ◽  
Aerobic Stability ◽  
Whole Plant ◽  
Fermentation Profile

Abstract We evaluated the effects of a chemical additive on the microbial communities, fermentation profile, and aerobic stability of whole-plant corn silage with or without air stress during storage. Whole-plant corn was either untreated or treated with a chemical additive containing sodium benzoate, potassium sorbate, and sodium nitrite at 2 or 3 liters/t of fresh forage weight. Ten individually treated and replicated silos (7.5 liters) were made for each treatment. Half of the silos remained sealed throughout a 63-d storage period, and the other half was subjected to air stress for 2 h/wk. The composition of the bacterial and fungal communities of fresh forage and silages untreated or treated with 2 liters/t of fresh forage weight was analyzed by Illumina Miseq sequencing. Treated silage had greater (P < 0.05) aerobic stability than untreated, even when subjected to air stress during storage, but the numbers of yeasts culturable on selective agar were not affected. However, the additive reduced the relative abundance (RA) of the lactating-assimilating yeast Candida tropicalis (P < 0.01). In air-stressed silages, untreated silage had a greater (P < 0.05) RA of Pichia kudriavzevii (also a lactate assimilator) than treated silage, whereas treated silage was dominated by Candida humilis, which is usually unable to assimilate lactate or assimilates it slowly. The additive improved the aerobic stability by specifically preventing the dominance of yeast species that can consume lactate and initiate aerobic spoilage. To the best of our knowledge, this is the first work that identifies the specific action of this additive on shifting the microbial communities in corn silage.

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