scholarly journals Influence of Physicochemical Characteristics of Bean Crop Soil in Trichoderma spp. Development

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 274
Author(s):  
Sara Mayo-Prieto ◽  
Alejandra J. Porteous-Álvarez ◽  
Sergio Mezquita-García ◽  
Álvaro Rodríguez-González ◽  
Guzmán Carro-Huerga ◽  
...  

Spain has ranked 6th on the harvested bean area and 8th in bean production in the European Union (EU). The soils of this area have mixed silt loam and sandy loam texture, with moderate clay content, neutral or acidic pH, rich in organic matter and low carbonate levels, providing beans with high water absorption capacity and better organoleptic qualities after cooking. Similar to other crops, it is attacked by some phytopathogens. Hitherto, chemical methods have been used to control these organisms. However, with the Reform of the Community Agrarian Policy in the EU, the number of authorized plant protection products has been reduced to prevail food security, as well as to be sustainable in the long term, giving priority to the non-chemical methods that use biological agents, such as Trichoderma. This study aimed to investigate the relative importance of various crop soil parameters in the adaptation of Trichoderma spp. autoclaved soils (AS) and natural soils (NS) from the Protected Geographical Indication (PGI) “Alubia La Bañeza—León” that were inoculated with Trichoderma velutinum T029 and T. harzianum T059 and incubated in a culture chamber at 25 °C for 15 days. Their development was determined by quantitative PCR. Twelve soil samples were selected and analyzed from the productive zones of Astorga, La Bañeza, La Cabrera, Esla-Campos and Páramo. Their physicochemical characteristics were different by zone, as the texture of soils ranged between sandy loam and silt loam and the pH between strongly acid and slightly alkaline, as well as the organic matter (OM) concentration between low and remarkably high. Total C and N concentrations and their ratio were between medium and high in most of the soils and the rest of the micronutrients had an acceptable concentration except for Paramo’s soil. Both Trichoderma species developed better in AS than in NS, T. velutinum T029 grew better with high levels of OM, total C, ratio C:N, P, K, Fe, and Zn than T. harzianum T059 in clay soils, with the highest values of cation exchange capacity (CEC), pH, Ca, Mg and Mn. These effects were validated by Canonical Correlation Analysis (CCA), texture, particularly clay concentration, OM, electrical conductivity (EC), and pH (physical parameters) and B and Cu (soil elements) are the main factors explaining the influence in the Trichoderma development. OM, EC, C:N ratio and Cu are the main soil characteristics that influence in T. velutinum T029 development and pH in the development of T. harzianum T059.

2019 ◽  
Vol 78 (1) ◽  
pp. 35-45 ◽  
Author(s):  
Beata Bosiacka ◽  
Helena Więcław ◽  
Paweł Marciniuk ◽  
Marek Podlasiński

Abstract The vegetation of protected salt meadows along the Baltic coast is fairly well known; however, dandelions have been so far treated as a collective species. The aim of our study was to examine the microspecies diversity of the genus Taraxacum in Polish salt and brackish coastal meadows and to analyse soil property preferences of the dandelion microspecies identified. In addition, we analysed the relations between soil properties and vegetation patterns in dandelion-supporting coastal meadows (by canonical correspondence analysis). The salt and brackish meadows along the Polish Baltic coast we visited were found to support a total of 27 dandelion microspecies representing 5 sections. Analysis of vegetation patterns showed all the soil parameters (C:N ratio, organic matter content, pH, concentration of Mg, P, K, electrolytic conductivity of the saturated soil extract ECe) to explain 32.07% of the total variance in the species data. The maximum abundance of most dandelion microspecies was associated with the highest soil fertility, moderate pH values and organic matter content, and with the lowest magnesium content and soil salinity. The exceptions were T. latissimum, T. stenoglossum, T. pulchrifolium and T. lucidum the occur-rence of which was related to the lowest soil fertility and the highest salinity. In addition, several microspecies (T. leptodon, T. gentile, T. haematicum, T. fusciflorum and T. balticum) were observed at moderate C:N ratios and ECe. Four other microspecies (T. infestum, T. cordatum, T. hamatum, T. sertatum) occurred at the lowest pH and organic matter content. The information obtained increases the still insufficient body of knowledge on ecological spectra of individual dandelion microspecies, hence their potential indicator properties.


Water ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1617 ◽  
Author(s):  
Alexandra Tiefenbacher ◽  
Gabriele Weigelhofer ◽  
Andreas Klik ◽  
Matthias Pucher ◽  
Jakob Santner ◽  
...  

Besides the importance of dissolved organic matter (DOM) in soil biogeochemical processes, there is still a debate on how agricultural intensification affects the leaching of terrestrial DOM into adjacent aquatic ecosystems. In order to close this linkage, we conducted a short-term (45 day) lysimeter experiment with silt loam and sandy loam undisturbed/intact soil cores. Mineral (calcium ammonium nitrate) or organic (pig slurry) fertilizer was applied on the soil surface with a concentration equivalent to 130 kg N ha−1. On average, amounts of leached DOC over 45 days ranged between 20.4 mg (silt loam, mineral fertilizer) and 34.4 mg (sandy loam, organic fertilizer). Both, mineral and organic fertilization of a silt loam reduced concentration of dissolved organic carbon (DOC) in the leachate and shifted its composition towards a microbial-like signature (BIX) with a higher aromaticity (Fi) and a lower molecular size (E2:E3). However, in sandy loam only mineral fertilization affected organic matter leaching. There, lowered DOC concentrations with a smaller molecular size (E2:E3) could be detected. The overall effect of fertilization on DOC leaching and DOM composition was interrelated with soil texture and limited to first 12 days. Our results highlight the need for management measures, which prevent or reduce fast flow paths leading soil water directly into aquatic systems, such as surface flow, fast subsurface flow, or drainage water.


Soil Research ◽  
2016 ◽  
Vol 54 (7) ◽  
pp. 880
Author(s):  
Mohammad Reza Chaichi ◽  
Marcus Turcios ◽  
Mina Rostamza

Non-ionic surfactants have been well researched as a tool to ameliorate water repellent conditions. However, few studies have evaluated the risks and benefits of non-ionic surfactant applications in wettable soil. The objective of this study was to evaluate the effects of a surfactant in modifying the wetting pattern in soils of different textures and organic matter contents. The experimental treatments consisted of (1) four different soil textures including sandy, sandy loam, sandy clay loam and silt loam, (2) four different organic matter contents (0.2, 0.7, 1.2 and 1.7% by weight), and (3) irrigation water treatments with or without surfactant (IrrigAid Gold). The experiment was carried out in Plexiglas boxes with one drip emitter under the soil surface. The results demonstrated the superiority of surfactant application on increasing water distribution in the soil profile for all soil textural classes. Silt loam texture had the highest side wetted area and wetting depth 45min after the initiation of irrigation. Upward capillary water movement and top wetted area significantly decreased in the surfactant treatment across all soil textures except in sandy soil. As organic matter content increased, top wetted area decreased. These findings clarified the potential ability of surfactant in increasing water infiltration in non-repellent soil in an in vitro system.


1990 ◽  
Vol 4 (2) ◽  
pp. 279-283 ◽  
Author(s):  
Michael R. Blumhorst ◽  
Jerome B. Weber ◽  
Len R. Swain

Field experiments were conducted on six loam and sandy loam soils to study the influence of various soil parameters on atrazine, cyanazine, alachlor, metolachlor, and pendimethalin efficacy. Herbicidal activity was highly correlated to the soil organic content. Humic matter content was equally or better correlated (r = 0.70 to 0.91) with herbicide bioactivity than was organic matter content (r = 0.66 to 0.84). Regression equations were determined which allow herbicide rate recommendations for 80% weed control to be calculated based on soil humic matter or organic matter levels.


2020 ◽  
Vol 12 (1) ◽  
pp. 143-153
Author(s):  
Risikat N. AHMED ◽  
Sesan M. SOBA ◽  
Mercy O. BAMIGBOYE ◽  
Kamoldeen A. AJIJOLAKEWU

The present research aimed at screening various soils within Ilorin metropolis for antibiotic producing actinomycetes. The objectives of the study were to determine physiochemical parameters of soils, the occurrence of actinomycetes in soils, antibacterial potentials and identity of isolates. Soil parameters such as pH, temperature, moisture, organic matter and soil type were evaluated following standard procedures. Selective isolation to determine the occurrence of actinomycetes was performed by soil dilution using pour plate technique on starch casein agar. Preliminary antibacterial screening against 10 clinical test bacteria was performed using cross streak method. All isolates were initially identified based on morphological and biochemical characteristics, while the most bioactive isolates were further identified by molecular means. The soils were alkaline, with temperatures between 29 °C and 31 °C, moisture was in range of 0.72 ± 0.07c and 6.62 ± 0.42b. Highest organic matter content was 32.13 ± 0.20a with soil types mostly loamy and sandy loam. Ten actinomycetes (SM1 - SM10) were isolated, with the most frequently occurring isolate being SM3 and SM5 (16.7%). SM5 was the most active, inhibiting 9 out of 10 tests, with the highest inhibition against Staphylococcus aureus 25923 (24 mm ± 0.15a). All isolates were identified as Streptomyces by morphology and biochemical tests. Based on nucleotide similarity searches and phylogeny, two bioactive Streptomycetes were suggested as novel strains and thus named as Streptomyces bottropensis UIL RNA (SM5) and S. flavoviridis UIL RNA(SM7), which may serve as promising sources of antibiotics. Actinomycetes from Ilorin metropolis demonstrated broad spectrum of antibacterial activity against clinical test bacteria.


2003 ◽  
Vol 21 (1) ◽  
pp. 11-15
Author(s):  
Todd L. Mervosh

Abstract The herbicides isoxaben (Gallery) and oryzalin (Surflan) are commonly used to prevent weed emergence around ornamental plants in nurseries and landscapes. Saturated batch experiments were conducted in the laboratory to generate equilibrium sorption isotherms for these herbicides in soils and container media. Soils consisted of [A] a sandy loam containing 1.24% organic matter (by wt) after sieving, and [B] a silt loam containing 1.36% organic matter (by wt) after sieving. Container media obtained from nurseries consisted of [C] a mixture of 50% peat and 50% sand (by vol) containing 18.5% organic matter (by wt) after sieving, and [D] a mixture of 70% bark, 25% peat, and 5% fine stone (by vol) containing 34.9% organic matter (by wt) after sieving. Sorption isotherms were generated from these data. Based on sorption coefficients (Kd, Kf), container mix D sorbed both herbicides to a much greater extent than did the other substrates. Oryzalin sorption was greater than isoxaben sorption in each soil and container mix. For oryzalin, organic carbon partition coefficient (Koc) values in the soils were greater than those in the container mixes. Compositional differences in organic matter between soils and container media may account for differences in herbicide sorption in these media.


Soil Research ◽  
2008 ◽  
Vol 46 (7) ◽  
pp. 636
Author(s):  
J. M. Xue ◽  
P. W. Clinton ◽  
R. Sands ◽  
T. W. Payn ◽  
M. F. Skinner

Biuret (C2H5N3O2) priming effect on mineralisation of native soil N has not been precisely quantified in previous studies, although it is a potential microbial activity regulator and slow-release N fertiliser. Following application of biuret at concentrations of 0 (B0) and 100 (B100) mg/kg (oven-dried) soil, we measured the dynamics of biuret-derived 15N in soil N pools, soil C mineralisation, and microbial biomass C in a sandy loam and a silt loam during a 112-day-long incubation to investigate the fate of biuret 15N and its effect on net mineralisation of native soil N. Biuret was decomposed faster in the sandy loam soil than the silt loam soil. In the sandy loam soil, the stabilised N pool was a strong sink for the biuret-derived 15N and accumulated about half of the applied 15N at the end of incubation. In the silt loam soil, 68% of the 15N applied was recovered in the NO3−-N pool and the stabilised N pool accumulated only about 25% of the applied 15N at the end of incubation. Biuret addition increased the turnover rate constant of soil organic matter and caused a real priming effect on net mineralisation of native soil N in both soils. The additional mineralisation of native soil N was 20.1 mg/kg (equivalent to 27.3 kg N/ha) in the sandy loam soil and 20.5 mg/kg (equivalent to 57.3 kg N/ha) in the silt loam soil. Biuret priming effect was related to the acceleration of soil organic matter decomposition by increased microbial activity at an early stage and the death/decay of microbes at a later stage of incubation. The native soil N released through the priming effect was partially from soil non-biomass organic matter and partially from soil microbial biomass.


2004 ◽  
Vol 3 (1) ◽  
pp. 316
Author(s):  
M. Saleem Akhtar ◽  
Tammo S. Steenhuis ◽  
Brian K. Richards ◽  
Murray B. McBride

2020 ◽  
Vol 7 (03) ◽  
Author(s):  
PREM PANDEY ◽  
G. C. SAGAR ◽  
SUNDARMAN SHRESTHA2 ◽  
HIRAKAJI MANANDHAR ◽  
RITESH K. YADAV ◽  
...  

Nine isolates of Trichoderma spp. were isolated from different agro- ecological regions of Nepal viz; Jumla, Palpa, Chitwan, Tarahara, Banke, Illam and Salyan and screened against Sclerotium rolfsii Sacc. Adreded soil borne phytopathogen causing collar rot of chickpea in chickpea; In-vitro efficacy of nine fungal antagonist (Trichoderma spp.) against Sclerotium rolfsii were screened. Pot experiment was done to find out the effective management of S. rolfsi through Tricoderma using different methods i.e. Seed treatment, soil drenching and soil application. All the tested isolates of Trichoderma spp. were found effective on mycelial growth inhibition and sclerotial parasitization of S. rolfsii. Trichoderma isolated from Palpa district showed maximum growth inhibition (%) of pathogen periodically after 48(93.78%), 72(96.00%), 96(97.96%) and 120(100.00%) hours of inoculation. Parasitized sclerotium showed minimum sclerotial germination on agar plates. Moreover, Trichoderma species isolated from Palpa districts showed second best percent mycelial growth inhibition periodically at 72(25.00%), 120(29.16%), 168(29.16%) and 216(29.16%).In pot experiment at 40 days after sowing, Seedling height was maximum in soil drenching with 30g per 100ml of water (22.27cm) and Mortality percentage of seedlings was least or highest disease control was observed in seed treated with 109cfu/ml (0.000%).


Agronomy ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1970
Author(s):  
Barbara Sawicka ◽  
Barbara Krochmal-Marczak ◽  
Piotr Pszczółkowski ◽  
Elżbieta Jolanta Bielińska ◽  
Anna Wójcikowska-Kapusta ◽  
...  

The experiment was conducted between 2015–2017 in the Research Station for Cultivar Testing in Uhnin (51°34′ N, 23°02′ E), in Luvisols developed from sandy loam soils. Soil samples for the tests of enzymatic activity were collected after the crop was harvested. The measurements included: the content of dehydrogenases, phosphatases, urease and protease, as well as total organic carbon, total nitrogen and mineral nitrogen in soil, based on standard methods. The research results point to changes in the enzymatic activity of light soil under the influence of varied nitrogen fertilization. In objects fertilized with this ingredient, the activity of the analysed enzymes was significantly higher than in the control soil, except for combinations fertilised with 150 kg ha−1 N characterised by the highest accumulation of N-NO3− in soil. The activity of dehydrogenases, phosphatases and urease changed as the nitrogen dose increased. The polynomial regression analysis enabled a better understanding of those dependences. In the case of dehydrogenases, phosphatases and urease, a third-degree curvilinear relation of enzymatic activity to nitrogen fertilisation was observed (a fourth-degree relation was found, with a coefficient R2 in those equations amounting to =0.958, 0.977, 0.979, respectively) and in the case of protease activity, a fourth-degree relation, with coefficient R2 = 0.989. However, soil acidity did not have a significant influence on either the enzymatic activity or physico-chemical characteristics of soil under the cultivation of sweet potatoes. The C:N ratio turned out to be significantly negatively related to the content of the enzymes dehydrogenase (Adh), phosphatase (AF), urease (AU) and protease (AP) as well as the content of total nitrogen, especially its ammonium form (N-NH4). The obtained results indicate the usefulness of research on enzymatic activity as an indicator of soil reaction to nitrogen fertilization and will enable maintenance of the optimal biological balance of cultivated soils.


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