scholarly journals The Survival and Parasitism Rate of Tamarixia radiata (Hymenoptera: Eulophidae) on Its Host Exposed to Beauveriabassiana (Ascomycota: Hypocreales)

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1496
Author(s):  
Luis Carlos Ramos Aguila ◽  
Komivi Senyo Akutse ◽  
Hafiza Javaria Ashraf ◽  
Bamisope Steve Bamisile ◽  
Jingyi Lin ◽  
...  
Keyword(s):  
Developmental Process ◽  
Extended Period ◽  
Diaphorina Citri ◽  
Parasitism Rate ◽  
Negative Interaction ◽  
Antagonistic Effects ◽  
Significant Difference ◽  
Fungus Beauveria Bassiana ◽  
Fungal Suspension ◽  
F1 Generation

The effect of the entomopathogenic fungus Beauveria bassiana (BB-12) on the biological characteristics of Tamarixia radiata parasitizing Diaphorina citri was studied under laboratory conditions. Twenty 3rd–5th instar nymphs were exposed to a single already-mated female parasitoid (1 day old) and removed after 24 h. Subsequently, the nymphs were sprayed at 1, 24 and 48 h post-exposure with 1 × 108 conidial mL−1 suspension. The percentage of parasitism recorded was 22%, 35% and 41% at 1, 24 and 48 h, respectively. The emergence rate varied between 28%, 51% and 49% at 1, 24 and 48 h, respectively. In a subsequent experiment, nymphs of D. citri were sprayed with 1 × 108 conidial mL−1 suspension and then exposed to T. radiata at 1, 24 and 48 h post-spraying to allow for parasitism to occur. The percentage of parasitism recorded was 18%, 27% and 28% at 1, 24 and 48 h, respectively, significantly lower than the parasitism rate recorded in the control (48%). The percentage of emergence varied between 24%, 44% and 45% at 1, 24 and 48 h, respectively. In both experiments, no significant difference was observed in the pre-adult duration (days) and the sex ratio of F1 generation. Meanwhile, significant differences were observed in the longevity of the F1 generation of females and males of T. radiata in a treatment consisting of spraying the fungal suspension and the control. Overall, the findings of the current study revealed a negative interaction between T. radiata and B. bassiana in controlling D. citri nymphs. This outcome is believed to be a result of the antagonistic effects of B. bassiana on the developmental process of the pre-adult stages of the parasitoid. However, our results also show that with a properly timed application (allowing parasitism to occur over an extended period of time before the application of the fungus), T. radiata could potentially be used in combination with B. bassiana for the successful biological control of D. citri. This should be carried out in order to minimize the potentially negative interactions between these two biological agents.

(Preprint)

2018 ◽  
Author(s):  
Natalia Banasik ◽  
Dariusz Jemielniak ◽  
Wojciech P?dzich
Keyword(s):  
Medical Condition ◽  
Extended Period ◽  
Well Being ◽  
Marginal Effect ◽  
Life Duration ◽  
Significant Difference ◽  
The Mean ◽  
Academic Teachers ◽  
Length Of Life ◽  
The Impact

BACKGROUND There have been mixed results of the studies checking whether prayers do actually extend the life duration of the people prayed for. Most studies on the topic included a small number of prayers and most of them focused on people already struggling with a medical condition. Intercessory prayer’s influence on health is of scholarly interest, yet it is unclear if its effect may be dependent on the number of prayers for a named individual received per annum. OBJECTIVE We sought to examine if there is a noticeable increased longevity effect of intercessory prayer for a named individual’s well-being, if he receives a very high number of prayers per annum for an extended period. METHODS We retrieved and conducted a statistical analysis of the data about the length of life for 857 Roman Catholic bishops, 500 Catholic priests, and 3038 male academics from the US, France, Italy, Poland, Brazil, and Mexico. We obtained information for these individuals who died between 1988 and 2018 from Wikidata, and conducted an observational cohort study. Bishops were chosen for the study, as they receive millions of individual prayers for well being, according to conservative estimates. RESULTS There was a main effect for occupation F(2, 4391) = 4.07, p = .017, ηp 2 = .002, with pairwise comparisons indicating significant differences between the mean life duration of bishops (M=30489) and of priests (M=29894), but none between the academic teachers (M=30147) and either of the other groups. A comparison analysis between bishops from the largest and the smallest dioceses showed no significant difference t(67.31)=1.61, p = .11. Our main outcome measure is covariance of the mean length of life in each of the categories: bishops, priests, academic teachers, controlled for nationality. CONCLUSIONS The first analysis proved that bishops live longer than priests, but due to a marginal effect size this result should be treated with caution. No difference was found between the mean length of life of bishops from the largest and the smallest dioceses. We found no difference between bishops and male academics. These results show that the impact of intercessory prayers on longevity is not observable.

scholarly journals Selection responses for egg production of Fayoumi and Rhode Island Red breeds

2016 ◽  
Vol 19 (1-2) ◽  
pp. 66-73
Author(s):  
ME Haque ◽  
GK Deb ◽  
MN Hasan ◽  
MH Ali
Keyword(s):  
Rhode Island ◽  
Genetic Gain ◽  
Egg Production ◽  
Management Practices ◽  
Selection Responses ◽  
Lower Productivity ◽  
Significant Difference ◽  
F2 Generation ◽  
Foundation Stock ◽  
F1 Generation

This experiment estimated the selection responses for egg production of Fayoumi (Fay) and Rhode Island Red (RIR) breeds. A total of 3000; belonging Fay 2000 & RIR 1000 day old chicks were collected from Egypt and Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka respectively, considered as foundation stock. The chicks were brooded for 5 weeks and reared up to 14 weeks of age providing standard feeding and management. After brooding, 640 Fay and 504 RIR pullets were reared in 40 and 36 individual pens respectively, considering as a family. Finally, 30 and 24 best families of Fay and RIR respectively were selected with 420 Fay and 288 RIR pullets at 15 weeks of age, on the basis of age at 1st egg lay, egg production and fertility. Salmonella and mycoplasma free eggs were collected on the basis of blood test (agglutinate test) from the selected families of both the breeds and hatched out for next generation. The experiment was continued up to 40 weeks of age for each generation. The study revealed that hen day egg production of Fay and RIR was higher in Foundation stock (64.39% in Fay and 68.54% in RIR) than that of F1 generation (58.6% in Fay and 46.8% in RIR) but no significant difference (P > 0.05) was observed for livability between Foundation stock and F1 generation. Reasons for lower productivity in F1 generation may be the poor management practices during growing period. In F2 generation, egg production was higher in both the breeds (64.09% in Fay and 62.05% in RIR) than in F1 generation. Egg production of RIR was not recorded for F3 generation, as their ability to adopt under farmers condition was very poor. However, in F3 generation, egg production of Fay was higher (65.82%) than in F2 generation. Genetic gain in Fay was 0.06%, 0.11% and 0.12% for F1, F2 and F3 generation, respectively. In case of RIR genetic gain was 0.07% in F1 and 0.18% in F2 generation. Due to selection slight genetic gain for egg production was obtained in Fay and RIR but further research with larger stock is needed for better understanding of selection responses of these breeds.Bangladesh J. of Livestock Res. 19(1-2): 66-73, Jan-Dec 2012

160 COMPARATIVE STUDY OF IN VITRO CULTURE MEDIA AND ASSISTED HATCHING TECHNIQUES ON MOUSE EMBRYOS

2017 ◽  
Vol 29 (1) ◽  
pp. 188
Author(s):  
N. C. Negota ◽  
L. P. Nethenzheni ◽  
M. L. Mphaphathi ◽  
D. M. Barry ◽  
T. L. Nedambale
Keyword(s):  
In Vitro Culture ◽  
Chorionic Gonadotropin ◽  
Culture Medium ◽  
Culture Media ◽  
Assisted Hatching ◽  
Hatching Rate ◽  
Significant Difference ◽  
Equine Chorionic Gonadotropin ◽  
F1 Generation

The in vitro culture media and assisted hatching techniques remain challenging obstacles to be utilised widely. Mechanical, chemical, enzymatic thinning, and laser-assisted techniques have been used previously but information is still lacking on its application in livestock. The aim of this study was to compare the effect of 2 in vitro culture media (Hamster F10 and TMC-199) and 4 (mechanical, chemical, enzymatic, and laser) assisted hatching techniques on blastocyst formation and hatching rate using murine embryos as a model. The C57/b and Balb/c breeds were raised until they reached maturity and bred naturally to produce F1 generation. The light in the breeding house was controlled at 14 h light and 10 h dark. Feed and water were provided ad libitum for the mice. Superovulation of females were stimulated using equine chorionic gonadotropin and human chorionic gonadotropin. The F1 generation was used for the collection of the 400 blastocysts and randomly allocated into 4 assisted hatching techniques. Blastocysts were paired into a group of 10 and replicated 4 times for each assisted hatching technique. The general linear model of SAS version 9.4 (SAS Institute Inc., Cary, NC, USA) was used to analyse the data. Assisted hatching techniques of laser, mechanical, enzymatic, and chemical yielded 46.9 ± 37.1, 51.1 ± 40.2, 39.1 ± 35.8, and 33.3 ± 4.5%, respectively, under in vitro culture of Hamster F10. The TCM-199, laser, mechanical, enzymatic, and chemical assisted hatching techniques yielded 56.3 ± 43.3, 52.6 ± 35.5, 49.2 ± 37.5, and 33.9 ± 35.5%, respectively, with a significant difference. There was no significant difference observed in assisted hatching techniques and Hamster F10 culture medium. However, the hatching rate of embryos for all techniques was higher when in vitro cultured in TCM than cultured in Hamster F10. Hatching rate of blastocysts increased from chemical, enzymatic, mechanical, and laser with response to Hamster F10 and TCM; thus, laser is a suitable assisted hatching technique with TCM-199.

scholarly journals Acmella oleracea (L) R. K. Jansen Reproductive Toxicity in Zebrafish: An In Vivo and In Silico Assessment

2019 ◽  
Vol 2019 ◽  
pp. 1-19 ◽  
Author(s):  
Gisele Custodio de Souza ◽  
Arlindo César Matias Pereira ◽  
Muller Duarte Viana ◽  
Adriana Maciel Ferreira ◽  
Ianna Dias Ribeiro da Silva ◽  
...  
Keyword(s):  
Embryonic Development ◽  
In Silico ◽  
Reproductive Toxicity ◽  
Control Group ◽  
The North ◽  
Significant Difference ◽  
Hydroethanolic Extract ◽  
Primary Compound ◽  
F1 Generation

The plant species Acmella oleracea L. is used in the north of Brazil for the treatment of a range of illnesses, such as tuberculosis, flu, cough, and rheumatism and as an anti-inflammatory agent; besides, hydroethanolic formulations with this species are popularly used as a female aphrodisiac agent. However, currently, there are no studies performed evaluating its effect on embryonic development. Hence, this research aimed to evaluate the effects of the hydroethanolic extract of A. oleracea (EHFAo) on the reproductive performance (parental) and embryonic development (F1 generation) of zebrafish, at concentrations of 50, 100, and 200 μg/L. Histopathology of parental gonads after 21 days of exposure to EHFAo reveals few alterations in the ovaries and testes, not impairing the reproduction; an increase of eggs deposition was observed in animals treated with EHFAo at the highest concentrations. Nevertheless, concerning the embryonic development of F1, teratogenic effects were observed including tail deformation, cardiac and yolk edema, scoliosis, and growth retardation; these alterations were more prominent in the groups born from progenitors exposed to the highest concentrations (100 and 200 μg/L.); but only the occurrence of yolk and cardiac edema had a statistically significant difference when compared to the control group. The chromatographic analysis shows that spilanthol (affinin) was the primary compound found in the EHFAo. Hence, in silico assessment was performed to evaluate the pharmacokinetic and toxicological properties of this molecule and 37 metabolites derived from it. Overall, our data show that the treatment caused no detrimental changes in progenitors regarding their gonads or fertility but caused some potentially teratogenic activity in embryos, which may be due to the action of spilanthol’s metabolites M3, M6, M7, M8, M16, M28, and M31.

scholarly journals Selection, assessment of virulence to Alphitobius diaperinus, and Pr1 enzyme production of Beauveria bassiana (Bals.) Vuill. isolates cultured at stress temperatures

2015 ◽  
Vol 36 (6) ◽  
pp. 3529
Author(s):  
Kelly Christiane Constanski ◽  
Janaina Zorzetti ◽  
Pedro Manuel Oliveira Janeiro Neves
Keyword(s):  
Beauveria Bassiana ◽  
Alphitobius Diaperinus ◽  
Control Programs ◽  
Colony Forming Units ◽  
Significant Difference ◽  
Before And After ◽  
Insect Mortality ◽  
Selection Test ◽  
High And Low Temperatures ◽  
Fungus Beauveria Bassiana

The entomopathogenic fungus Beauveria bassiana is a promising agent for use in insect control. Its pathogenic activity, as well as other factors such as temperature that can interfere with its development, should be assessed, thus, establishing the foundations for B. bassiana use in biological control programs. The objective of this study was to select and induce tolerance of B. bassiana isolates to high and low temperatures and to assess their virulence before and after exposure to those temperatures. A pre-selection test was performed, in which the tolerance of isolates to stress temperatures was tested and compared to the ideal growth temperature of 25 °C for this organism. For the isolates/temperature combinations resulting in growth, conidia germination and colony-forming units (CFUs) were assessed. The isolates Unioeste 4 and Unioeste 40 exhibited >95% germinated conidia at 16 and 31 °C. Thereafter, they underwent four consecutive passages at maximum and minimum tolerated temperatures (10 and 37 °C). A significant difference in germination was observed between the two isolates at all temperatures tested. More CFUs were observed for Unioeste 4 compared to Unioeste 40 at all temperatures, and in the case of the latter, there was no difference in CFU formation at 10 and 25 °C. For both isolates, decreased vegetative growth was observed at 37 °C. Recovery of virulence was observed in both isolates, as determined by insect mortality. No relationship was observed between production of the enzyme Pr1 and the virulence of the isolates.

scholarly journals Interaction between the entomopathogenic fungus, Beauveria bassiana (Ascomycota: Hypocreales) and the parasitoid wasp, Aphidius colemani Viereck (Hymenoptera: Braconidae)

2013 ◽  
Vol 45 (1) ◽  
pp. 4 ◽  
Author(s):  
F. Emami ◽  
M. Alichi ◽  
K. Minaei
Keyword(s):  
Beauveria Bassiana ◽  
Myzus Persicae ◽  
Entomopathogenic Fungus ◽  
Green Peach Aphid ◽  
Parasitoid Wasp ◽  
Lethal Concentration ◽  
Aphidius Colemani ◽  
Parasitoid Development ◽  
Fungus Beauveria Bassiana ◽  
F1 Generation

The effect of the entomopathogenic fungus, <em>Beauveria bassiana</em>, on the biological characteristics of <em>Aphidius colemani</em>, a parasitoid of the green peach aphid, <em>Myzus persicae</em>, was studied under laboratory conditions. Third-instar nymphs of green peach aphid were infected with 5/3&times;105 conidia/mL of <em>B. bassiana</em>, which was determined to be the lethal concentration 50 dose. They were then offered to mated female parasitoids for 24 h at different intervals. Results showed that by prolonging the release intervals of parasitoids, the number of mummies and percent emergence of parasitoids were reduced. Moreover, production of male offspring increased in the F1 generation of parasitoids. The interference of <em>B. bassiana</em> with parasitoid development was also studied by first exposing the aphid hosts to the parasitoids for 24 h and subsequently spraying them with <em>B. bassiana</em> 24, 48, 72, and 96 h after exposure. Results showed that by prolonging fungal spraying intervals, the number of mummies and percent emergence of parasitoids were increased. It appeared that the best time for applying <em>B. bassiana</em> would be three to four days after parasitisation.

The artificial rearing of calves and their growth on grass diets: II. The effect of length of period of feeding cold milk substitute to spring–born calves at pasture

1969 ◽  
Vol 73 (3) ◽  
pp. 483-488 ◽  
Author(s):  
C. R. Lonsdale ◽  
J. C. Tayler
Keyword(s):  
Compensatory Growth ◽  
Live Weight ◽  
Extended Period ◽  
Winter Period ◽  
Artificial Rearing ◽  
Significant Difference ◽  
Weight Gains ◽  
Milk Substitute ◽  
Reconstituted Milk ◽  
Milk Feeding

SummaryAn experiment was conducted with 32 spring-born Friesian heifer calves, purchased at an average age of 10 days and fed on cold reconstituted milk substitute at pasture.The milk feeding treatments were 4, 4½, 6½ and 9½ weeks in length, during which time calves consumed an average of 17, 23, 43 and 70 kg dry matter/head of milk substitute respectively. The differences between treatment mean live-weight gains for 10½ weeks of the experiment were highly significant (P < O·OOl): the gains were 0·49, 0·43, 0·64 and 0·78 kg/head/day. There was no significant difference between mean rates of live-weight gain after the tenth week so that the overall treatment—mean live-weight gains for the first 23 weeks, which were 0·65, 0·61, 0·72 and 0·74 kg/head/day, strongly reflected the length of the milk-feeding period and demonstrated no significant compensatory growth by the calves.Twenty of these animals were housed for the subsequent winter period and were returned to pasture for a second grazing season. These were artificially inseminated as their individual live weights reached approximately 320 kg. Animals weaned after only 4 weeks of milk feeding took an average of 9½ weeks longer to reach live weights of 320 kg than these receiving milk for 9½ weeks. There were no marked indications of compensatory growth during this extended period of observations.

scholarly journals The effect of genotype on dressing percentage and tissue content of beef carcasses

2007 ◽  
Vol 23 (5-6-2) ◽  
pp. 17-23 ◽  
Author(s):  
D. Ostojic-Andric ◽  
V. Bogdanovic ◽  
S. Aleksic ◽  
M.M. Petrovic ◽  
B. Miscevic ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Bone Tissue ◽  
Muscle Tissue ◽  
Genetic Group ◽  
Tissue Content ◽  
Beef Carcasses ◽  
Significant Difference ◽  
Dressing Percentage ◽  
Different Tissues ◽  
F1 Generation

Differences in dressing percentage and content of different tissues in carcasses of Domestic Spotted breed and crosses F1 generation with Charolais and Limousine breed were investigated. Total of 30 carcass sides were dissected, 10 in each genetic group. Genotype exhibited very significant effect (p<0,01) on dressing percentage and content of tissues, except for connective tissue. The highest dressing percentage was determined in crosses with Charolais (59,92%) followed by crosses with Limousine (59,85%), with statistically significant difference (p<0,01) to dressing percentage established for heads of domestic Simmental breed (55,71%). Crosses with Charolais and Limousine realized considerably higher content of muscle tissue (81,5% and 80,7%) compared to heads of domestic Simmental breed (77,86%). Content of bone tissue of 16,45% in carcasses of domestic Simmental breed was considerably higher (p<0,01) compared to both groups of crosses, whereas the content of fat and connective tissue was considerably higher (p<0,05) only in relation to crosses with Limousine.

The dynamics of word retrieval in major depression

2012 ◽  
Vol 47 (3) ◽  
pp. 276-283 ◽  
Author(s):  
Katie M Douglas ◽  
Richard J Porter ◽  
Robert G Knight ◽  
Brent Alsop
Keyword(s):  
Major Depression ◽  
Autobiographical Memory ◽  
Time Course ◽  
Antidepressant Medication ◽  
Extended Period ◽  
General Characteristic ◽  
Long Term Memory ◽  
Retrieval Processes ◽  
Depressed Group ◽  
Significant Difference

Objective: Impairment in the retrieval of specific episodes from autobiographical memory is commonly observed in major depression. However, it is unclear whether impairment in retrieval processes is a general characteristic of major depression or is confined to the recollection of personal memories. This study examined the time course of the retrieval of words from semantic memory. Method: A letter fluency test was administered to 65 inpatients with major depression and 50 healthy controls. A two-parameter model was fit to the decay curve representing the production of words over a 90-second period. One parameter, N, is an estimate of the total number of words that would be generated if the respondent was given unlimited time. The other, tau, is the average of the difference in time between the first word generated and each subsequent word. Results: There was evidence of a deficit in the retrieval of words from long-term memory in depressed patients. The significant difference between groups suggested that even if given an extended period of time in which to respond to compensate for possible slowness, the depressed group would not retrieve as many words as the controls. The retrieval failure could not be attributed solely to cognitive slowing or the effects of antidepressant medication. Conclusions: The results extend findings of a deficit in the process of retrieving specific episodes from autobiographical memory and suggest that a generalised impairment in memory retrieval may be characteristic of major depression.

Withdrawal of Neuroleptic Medications from Institutionalized Dementia Patients: Results of a Double-Blind, Baseline-Treatment—Controlled Pilot Study

1997 ◽  
Vol 10 (3) ◽  
pp. 119-126 ◽  
Author(s):  
Sarah Bridges-Parlet ◽  
David Knopman ◽  
Susan Steffes
Keyword(s):  
Aggressive Behavior ◽  
Objective Assessment ◽  
Extended Period ◽  
Good Clinical Practice ◽  
Chi Square ◽  
Double Blind ◽  
Term Care ◽  
Dementia Patients ◽  
Significant Difference ◽  
The Difference

Among dementia patients in long-term care facilities, neuroleptics (NL) are frequently prescribed for the treatment of agitation. Although good clinical practice and federal law mandate attempts at withdrawal of these medications, empiric data regarding the cessation of NL treatment are limited in this population. The objective of the present study was to assess through direct observation the effects of short-term NL withdrawal on physically aggressive behavior and other aspects of agitation. We carried out a randomized, double-blind, baseline NL-controlled 4-week trial of the effects of NL withdrawal in 36 institutionalized patients with possible or probable Alzheimer's disease. Patients were directly observed for four 2-hour sessions during baseline, a prerandomization week, and during weeks 1, 2, and 4 of the double-blind portion of the study. Completion of the 4 weeks of double-blind medication and number of observed episodes of physically aggressive behavior (PAB) were the two primary outcome measures. Of the 22 patients who were withdrawn from NL, 20 (91%) completed the 4-week double-blinded withdrawal. Two patients were discontinued from the study due to unacceptable levels of agitation at the request of their nursing staff. Of the 14 patients not withdrawn, all completed the 4-week trial. The chi-square test for the difference between groups was not significant ( P > .05). Based on the observed instances of PAB, there was no significant difference ( P > .05) between withdrawn and not-withdrawn subjects. Half of the withdrawn patients remained off NLs for an extended period of time after the end of the study, even after the blind was broken. Withdrawing institutionalized dementia patients from NLs was successful in most but not all study patients. Generalization of these results is limited by the highly selected nature of the participants. Unmasking of unmanageable agitation and physical aggressiveness in a small minority must be weighed against the benefits of removing unnecessary medication in the majority of dementia patients in whom NL withdrawal is attempted. PAB itself should not drive continuing NL use without regard to objective assessment of efficacy of the NL treatment.

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