Dietary ARA Improves COX Activity in Broodstock and Offspring Survival Fitness of a Model Organism (Medaka Oryzias latipes)

A 3-week feeding trial was conducted in medaka broodstock (age five months) to examine the effect of dietary arachidonic acid (ARA) level (range: 4–23 mg g−1 of total fatty acids (TFAs)) on fertility, cyclooxygenase (COX) activity, egg size, sperm motility parameters, hatching rate and weight of hatch, survival and growth of larvae. After spawn induction and dietary exposure to 4 mg ARA g−1 TFA, broodstock were fed a diet containing ARA in the amounts: 4 (continued, as control), 5, 14 and 23 mg g−1 TFA. COX1 activity in the liver and the number of COX2-positive cells in the ovaries was increased in females fed the diets containing the two highest amounts of ARA. The highest sperm motility parameters were observed in males fed a diet containing 23 mg ARA g−1 TFA. The hatchability rate and bodyweight of hatchlings were higher in the group obtained from broodstock fed a diet containing 23 mg ARA g−1 TFA (79% and 0.66 mg fish−1, respectively) compared with 4 mg ARA g−1 TFA (50% and 0.40 mg fish−1). The average mortality of offspring obtained from this group at 7 days post hatching was significantly higher than that of all other groups.

Download Full-text

Role of Ca2+ in the IVM of spermatozoa from the sterlet Acipenser ruthenus

Reproduction Fertility and Development ◽

10.1071/rd16145 ◽

2017 ◽

Vol 29 (7) ◽

pp. 1319 ◽

Cited By ~ 4

Author(s):

Olga Bondarenko ◽

Borys Dzyuba ◽

Marek Rodina ◽

Jacky Cosson

Keyword(s):

Sperm Motility ◽

Seminal Fluid ◽

Mature Male ◽

Sperm Maturation ◽

Wolffian Duct ◽

Testicular Spermatozoa ◽

Acipenser Ruthenus ◽

Sterlet Acipenser Ruthenus ◽

Motility Parameters

The role of Ca2+ in sturgeon sperm maturation and motility was investigated. Sperm from mature male sterlets (Acipenser ruthenus) were collected from the Wolffian duct and testis 24 h after hormone induction. Testicular spermatozoa (TS) were incubated in Wolffian duct seminal fluid (WDSF) for 5 min at 20°C and were designated ‘TS after IVM’ (TSM). Sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. To investigate the role of calcium transport in the IVM process, IVM was performed (5 min at 20°C) in the presence of 2 mM EGTA, 100 µM Verapamil or 100 µM Tetracaine. No motility was observed in the case of TS (10 mM Tris, 25 mM NaCl, 50 mM Sucr with or without the addition of 2 mM EGTA). Both incubation of TS in WDSF and supplementation of the activation medium with Ca2+ led to sperm motility. The minimal Ca2+ concentration required for motility activation of Wolffian duct spermatozoa, TS and TSM was determined (1–2 nM for Wolffian duct spermatozoa and TSM; approximately 0.6 mM for TS). Motility was obtained after the addition of verapamil to the incubation medium during IVM, whereas the addition of EGTA completely suppressed motility, implying Ca2+ involvement in sturgeon sperm maturation. Further studies into the roles of Ca2+ transport in sturgeon sperm maturation and motility are required.

Download Full-text

Characteristics of Siberian sturgeon and sterlet sperm motility parameters compared using CASA

Archives of Polish Fisheries ◽

10.2478/v10086-012-0016-0 ◽

2012 ◽

Vol 20 (2) ◽

Cited By ~ 4

Author(s):

Piotr Sieczyński ◽

Jan Glogowski ◽

Beata Cejko ◽

Cezary Grygoruk

Keyword(s):

Sperm Motility ◽

Siberian Sturgeon ◽

Motility Parameters

Download Full-text

ANALISIS KARAKTER REPRODUKSI DAN PERFORMA BENIH HIBRID IKAN NILA PANDU F6 DENGAN IKAN NILA NILASA (Oreochromis niloticus) Analysis of the Character Reproduction and Seed Hybrid Performance 0f Tilapia Fish F6 Pandu with Nilasa Tilapia (Oreochromis niloticus)

SAINTEK PERIKANAN Indonesian Journal of Fisheries Science and Technology ◽

10.14710/ijfst.12.1.19-23 ◽

2017 ◽

Vol 12 (1) ◽

pp. 19

Author(s):

Dio Patria Yustysi ◽

Fajar Basuki ◽

Titik Susilowati ◽

Tristiana Yuniarti

Keyword(s):

Water Quality ◽

Growth Rate ◽

Survival Rate ◽

Specific Growth Rate ◽

Oreochromis Niloticus ◽

Egg Size ◽

Specific Growth ◽

Fish Farming ◽

Hatching Rate ◽

Feed Conversion

ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh dari hibridisasi ikan nila Pandu F6 dengan nila Nilasa terhadap karakter reproduksinya dan performa benih yang dihasilkan. Penelitian ini dilakukan dengan metode eksperimental menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 kali ulangan. Perlakuan dalam penelitian ini: perlakuan A (Pandu F6 ♀><Pandu F6 ♂), B (Nilasa ♀><Nilasa ♂), C (Pandu F6 ♀>< Nilasa ♂), dan D (Nilasa ♀><Pandu F6 ♂). Data yang diamati meliputi fekunditas, daya tetas telur, diameter dan bobot telur, panjang dan bobot larva TL, panjang dan bobot larva lepas kuning telur, kelulushidupan, laju pertumbuhan spesifik, konversi pakan dan kualitas air. Hasil penelitian menunjukan nilai terbaik pada fekunditas sebesar 1191,67 ± 239,45 butir/200 g, daya tetas telur sebesar 80,93 ± 3,90%, kelulushidupan sebesar 81,55 ± 7,54%, laju pertumbuhan spesifik sebesar 7,26 ± 0,25, konversi pakan 0,54 ± 0,02 didapatkan pada perlakuan C, akan tetapi untuk diameter dan bobot telur, panjang dan bobot larva Kuning Telur, dan panjang dan bobot larva lepas kuning telur tidak berbeda nyata. Hasil tersebut menunjukan bahwa hibridisasi (perlakuan C) memberikan pengaruh yang nyata (P < 0,05) terhadap fekunditas, daya tetas telur, kelulushidupan, laju pertumbuhan spesifik, dan konversi pakan tetapi tidak berpengaruh terhadap diameter dan bobot telur, panjang dan bobot larva, dan panjang dan bobot larva lepas kuning telur. Kata kunci: Hibridisasi, Ikan Nila, Karakter Reproduksi, Performa Benih ABSTRACT This research aims to know the influence of hybridization tilapia fish Pandu F6 with nila Nilasa against the character of the reproduction and seed performance. This research was conducted with the experimental method using Random Design complete (RAL) with 4 treatments and three replicates. The treatments in this study: the treatment A (Pandu F6 F6 ♀ >< Pandu ♂), B (Nilasa ♀>< Nilasa ♂), C (Pandu F6 ♀>< Nilasa ♂), and D (Nilasa ♀ >< Pandu F6 ♂). The observed data covering fecundity, hatching rate, egg size, yolk sack larva length and weight, length and weight of the egg yolk off larvae, survival rate, specific growth rate, feed conversion rate, and water quality. The results showed the best value on the fecundity of 1191.67±239.45 eggs/200 g, Hatching rate 80.93 ± 3.90%, Survival rate of 81.55 ± 7.54%, specific growth rate of 7.26 ± 0.25, conversion fodder 0.54 ± 0.02 obtained at the treatment C, but for the diameter and weight of egg, larval length and weight TL, and the length and weight of the yolk egg off larvae for each treatment do not differ markedly. The results showed that hybridization (treatment C) gives a real influence (P < 0.05) of fecundity,hatching rate, survival rate but not with the egg size, larva weight and length, and the length and weight of larvae off yolk. Water quality on the spawning, hatching eggs and larvae found on the maintenance of a decent range for tilapia fish farming Keywords: hybridization, tilapia fish, character of the reproduction, seed performance

Download Full-text

OPTIMAL ELECTROPORATION CONDITION FOR SPERM MEDIATED GENE TRANSFER IN STRIPPED CATFISH (Pangasionodon hypophthalmus)

Indonesian Aquaculture Journal ◽

10.15578/iaj.5.1.2010.1-10 ◽

2010 ◽

Vol 5 (1) ◽

pp. 1

Author(s):

Raden Roro Sri Pudji Sinarni Dewi ◽

Alimuddin Alimuddin ◽

Agus Oman Sudrajat ◽

Komar Sumantadinata ◽

Sularto Sularto

Keyword(s):

Sperm Motility ◽

Fluorescent Protein ◽

Pulse Length ◽

Transgenic Fish ◽

Pulse Number ◽

Pulse Interval ◽

Hatching Rate ◽

Exogenous Dna ◽

Motility Of Sperm ◽

Green Fluorescent

The success of transgenic fish production has been achieved through eggs fertilization using electroporated sperms carrying exogenous DNA. This study was conducted in order to obtain the optimal electroporation condition for stripped catfish sperm. A plasmid containing green fluorescent protein (GFP) gene driven by carp β-actin promoter was transferred into sperm using electrophoresis method towards transgenic stripped catfish (Pangasionodon hypophthalmus) production. Electroporation was carried out using square wave shock with pulse length of 30 ms and pulse interval of 0.1 sec. Treatments are combination between voltage (50 V, 75 V, and 100 V) and pulse number (1 and 3). Exogenous DNA concentration used was 10 μg/mL of Tris-EDTA. Results showed that increasing the voltage from 50 to 100 decreased sperm motility, while pulse number did not affect sperm motility. Voltage of 50 gave the best motility of sperm, although sperm viability relatively similar between treatments and control except at 100 V with 3 pulses number. Further, electroporation-treated sperms were able to fertilize eggs. Higher hatching rate of eggs was obtained in electroporation treatment at 50 V with pulse number of 1 and 3. The persistence of transferred GFP was detected in electroporated and incubated sperms (control). However, GFP was only detected in larvae from eggs that were fertilized by electroporated sperm. Thus, electroporation could be applied to produce transgenic stripped catfish.

Download Full-text

Preliminary Study Rearing of Oryzias sp. from Tunda Island, Indonesia, Under Laboratory Condition

Musamus Fisheries and Marine Journal ◽

10.35724/mfmj.v2i1.1872 ◽

2019 ◽

pp. 24-34

Author(s):

Muh. Herjayanto ◽

Annisa Misykah Mauliddina ◽

Esa Rama Widiyawan ◽

Nugroho Agung Prasetyo ◽

Lukman Anugrah Agung ◽

...

Keyword(s):

Laboratory Condition ◽

Model Organism ◽

Ornamental Fish ◽

Fish Mortality ◽

Controlled Environment ◽

Hatching Rate ◽

Basic Information ◽

Hatching Process ◽

Preliminary Study

Oryzias sp. from Tunda Island, Indonesia has the potential as a model organism in laboratories and also ornamental fish. Basic information regarding maintenance during adaptation post-transportation from its habitat in nature, embryo incubation and maintenance of larvae is needed as a basis for breeding activities. The results showed that Oryzias sp. from Tunda Island can adapt, spawning and produce a total 262 eggs (diameter 0.88-1.02 mm) in a controlled environment. Eggs from spawning in nature begin to hatch on the 11th incubation day, the hatching peak occurs on the 13th day with a hatching process 4-9 days at 25-27oC. The hatching rate ranges from 57.14-100% and the survival of 5 days old lavae after hatching is 72.72-100.00%. The transportation technique in this study has not been able to suppress fish mortality during post-transportation rearing.

Download Full-text

Impacts of dietary exposure to sodium or potassium salts of nitrate and nitrite on the development of Drosophila melanogaster

Interdisciplinary Toxicology ◽

10.1515/intox-2017-0012 ◽

2017 ◽

Vol 10 (2) ◽

pp. 70-78 ◽

Cited By ~ 1

Author(s):

Ashim Kumar Basak ◽

Tridip Chatterjee ◽

Swapan Kumar Ghosh ◽

Amit Chakravarty

Keyword(s):

Young Adults ◽

Food Additives ◽

Polytene Chromosomes ◽

Model Organism ◽

Developmental Outcomes ◽

Dietary Exposure ◽

Potassium Nitrate ◽

Toxicological Studies ◽

Nitrate And Nitrite ◽

Third Instar Larvae

AbstractThe effects of four food additives, namely sodium nitrite (NaNO2), sodium nitrate (NaNO3), potassium nitrite (KNO2), and potassium nitrate (KNO3), on animal development were evaluated by using Drosophila melanogster, a model organism. Adult male and female flies were allowed to breed in culture medium, each containing one of 4 concentrations,i.e.10, 20, 30 or 40 mM of the above mentioned salts. The concentration of 40 mM, NaNO2and KNO2 completely arrested the development of the flies. Of the different concentrations of the four salts tested, exposure of flies to 30 mM NaNO2exhibited only significant delays in the initial appearances of third instar larvae, pupae and young adults, along with huge reduction in the number of pupae and young adults compared to controls. Rearrangements like inversions, deletion looping, regional shrinking, as well as highly enlarged puffing,etc.were also observed in the polytene chromosomes of the third instar larvae exposed to 30 mM NaNO2. Developmental outcomes of the flies exposed to varying concentrations of NaNO3and KNO3 did not differ significantly from the controls. Owing to the extensive genetic homology between Drosophila and human and the successful uses of this fly as models in developmental and toxicological studies, we speculate that the experimental results exhibited by this organism in our study strongly advocate for abstaining from the dietary use of NaNO2and KNO2 during human pregnancies to avoid possible negative developmental outcomes.

Download Full-text

Zebrafish (Danio rerio) and the egg size versus egg number trade off: effects of ration size on fecundity are not mediated by orthologues of the Fec gene

Reproduction Fertility and Development ◽

10.1071/rd09257 ◽

2010 ◽

Vol 22 (6) ◽

pp. 1015 ◽

Cited By ~ 8

Author(s):

Erin L. Forbes ◽

Craig D. Preston ◽

P. Mark Lokman

Keyword(s):

Danio Rerio ◽

Egg Size ◽

Egg Quality ◽

Quantitative Polymerase Chain Reaction ◽

Gonadosomatic Index ◽

Hatching Rate ◽

Mrna Levels ◽

Trophic Conditions ◽

Trade Off ◽

Ration Size

Few studies have demonstrated plasticity of egg size within the confines of an egg size–number trade-off in response to trophic conditions in fishes. Moreover, the physiological mechanisms that govern this plasticity are not known. Growth differentiation factor 9 (Gdf9) and bone morphogenetic factor 15 (Bmp15) are oocyte-specific factors implicated in follicular growth and ovulation in mammals. In order to investigate whether expression levels of these genes were correlated with ration-dependent changes in fecundity in fish, zebrafish (Danio rerio) were subjected to four different feeding regimens. Counts of spawned eggs or vitellogenic follicles were used to estimate fecundity, whereas quantitative polymerase chain reaction analyses were performed to determine Gdf9 and Bmp15 mRNA levels in response to changes in ration size. Both relative fecundity and gonadosomatic index increased significantly with increased ration size, whereas egg size and hatching rate decreased significantly. No significant differences in Gdf9 or Bmp15 transcript abundance were evident between feeding regimens, suggesting that these growth factors do not govern fecundity in fish. However, favourable trophic conditions markedly affected follicle or egg size and number, with important implications for downstream egg quality and survival.

Download Full-text

Effect of albumin and casein supplementation on the common carp Cyprinus carpio L. sperm motility parameters measured by CASA

Aquaculture International ◽

10.1007/s10499-013-9673-2 ◽

2013 ◽

Vol 22 (1) ◽

pp. 123-129 ◽

Cited By ~ 7

Author(s):

Radosław Kajetan Kowalski ◽

Beata Irena Cejko ◽

Sławomir Krejszeff ◽

Beata Sarosiek ◽

Sylwia Judycka ◽

...

Keyword(s):

Common Carp ◽

Sperm Motility ◽

Cyprinus Carpio ◽

Common Carp Cyprinus Carpio ◽

Carp Cyprinus Carpio ◽

The Common ◽

Motility Parameters

Download Full-text

Effects of Pyriproxyfen Exposure on Reproduction and Gene Expressions in Silkworm, Bombyx mori

Insects ◽

10.3390/insects11080467 ◽

2020 ◽

Vol 11 (8) ◽

pp. 467

Author(s):

He-Ying Qian ◽

Xiao Zhang ◽

Guo-Dong Zhao ◽

Hui-Min Guo ◽

Gang Li ◽

...

Keyword(s):

Bombyx Mori ◽

Model Organism ◽

Ovary Development ◽

Control Group ◽

Hatching Rate ◽

Hormone Regulation ◽

Concentration Effects ◽

Gene Expressions ◽

Silkworm Larvae ◽

Silkworm Bombyx Mori

The silkworm, Bombyx mori Linnaeus, is an important economic insect and a representative model organism of Lepidoptera, which has been widely used in the study of reproduction and development. The development of the silkworm’s reproductive gland is easily affected by many external factors, such as chemical insecticides. After the silkworm larvae were treated with different concentrations of pyriproxyfen, the results showed that the number of eggs and hatching rate of eggs in the silkworm can be reduced by pyriproxyfen, and the concentration effects were displayed. Pyriproxyfen exposure could affect the normal development of the ovary tissue by reducing the number of oocytes and oogonia in the ovaries of silkworm fed with pyriproxyfen. We employed qRT-PCR, to detect the expressions of genes related to ovary development (Vg, Ovo, Otu, Sxl-S and Sxl-L) and hormone regulation (EcR and JHBP2) in silkworm. Our study showed that the transcription levels of Vg, Ovo, Otu, Sxl-S and Sxl-L in the treatment group were lower than those in the control group (6.08%, 61.99%, 83.51%, 99.31% and 71.95%, respectively). The transcription level of ECR was 70.22% for the control group, while that of JHBP2 was upregulated by 3.92-fold. Changes of transcription levels of these genes caused by pyriproxyfen exposure ultimately affect the absorption of nutrients, energy metabolism, ovary development and egg formation of the silkworm, thus leading to reproductive disorders of the silkworm. In general, our study revealed the response of silkworm reproduction to pyriproxyfen exposure and provided a certain reference value for the metabolism of the silkworm to pyriproxyfen.

Download Full-text