scholarly journals Performance and Milk Composition of Nubian Goats as Affected by Increasing Level of Nannochloropsis oculata Microalgae

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2453
Author(s):  
Ahmed E. Kholif ◽  
Gouda A. Gouda ◽  
Hatem A. Hamdon
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Nutritive Value ◽  
Unsaturated Fatty Acids ◽  
Ammonia Nitrogen ◽  
Nannochloropsis Oculata ◽  
Nutrient Digestibility ◽  
Atherogenic Index ◽  
Control Treatment ◽  
Lactating Goats

Fat supplementation affects the lactational performance of goats and dramatically changes milk nutritive value. In the present experiment, two levels of Nannochloropsis oculata microalgae, a natural source of rumen-protected eicosapentaenoic acid (EPA), were studied in the diet of Nubian goats. Using quintuplicated 3 × 3 Latin square design, fifteen lactating goats, (14 ± 2 months old and 33.0 ± 1.3 kg) after kidding, were randomly assigned into three treatments in an 84-d assay. Goats were offered a basal diet comprising berseem clover, wheat straw and concentrates in 3:2:5, respectively, (control treatment-no supplementation). The other two treatments were supplemented with N. oculata microalgae at 5 g (NOM5 treatment) or 10 g (NOM10 treatment)/doe/d. Without affecting intake, treatments improved (p < 0.01) nutrient digestibility. Supplementations had no effect on ruminal pH and ammonia-nitrogen, however, NOM5 and NOM10 linearly improved (p < 0.05) total volatile fatty acids and propionic acids. N. oculata supplementation linearly increased (p < 0.01) milk yield and lactose content. Supplementation reduced atherogenic index (p = 0.004) and enhanced the concentrations of unsaturated fatty acids and C20:5n3 (EPA). Conclusively, feeding Nubian goats on diet supplemented with N. oculata at 5 and 10 g improved milk production and the nutritive value. No improvements in the performance were observed when N. oculata dose was increased from 5 g to 10 g/doe; thus, 5 g dose is recommended for use.

scholarly journals Insects as Novel Ruminant Feed and a Potential Mitigation Strategy for Methane Emissions

Animals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2648
Author(s):  
Eslam Ahmed ◽  
Naoki Fukuma ◽  
Masaaki Hanada ◽  
Takehiro Nishida
Keyword(s):  
Fatty Acids ◽  
Methane Production ◽  
Volatile Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Ammonia Nitrogen ◽  
Nutrient Digestibility ◽  
Acheta Domesticus ◽  
Environmental Benefit ◽  
Grass Hay

This study is the first to evaluate the chemical composition and impacts of four different edible insects, Acheta domesticus (A.d), Brachytrupes portentosus (B.p), Gryllus bimaculatus (G.b), and Bombyx mori (B.m), on the digestibility, rumen fermentation, and methane production when used as a substitute for 25% of the soybean meal (SBM) in a ruminant diet through in vitro incubation. The dietary treatments were 100% grass hay, 60% grass hay + 40% SBM, 60% grass hay + 30% SBM + 10% A.d, 60% grass hay + 30% SBM + 10% B.p, 60% grass hay + 30% SBM + 10% G.b, and 60% grass hay + 30% SBM + 10% B.m. The experiment was conducted as a short-term batch culture for 24 h at 39 °C, and the incubation was repeated in 3 consecutive runs. Chemical analysis of the insects showed that they were rich in fat (14–26%) with a high proportion of unsaturated fatty acids (60–70%). Additionally, the insects were rich in protein (48–61%) containing all essential amino acids and the amino acid profiles of the insects were almost the same as that of SBM. The inclusion of insects did not affect nutrient digestibility or the production of volatile fatty acids but did increase the production of ammonia-nitrogen. The addition of G.b and B.m led to decrease in methane production by up to 18% and 16%, respectively. These results reveal that substitution of 25% SBM in the diet with the tested insects had no negative impacts, and their potential to reduce methane production is an environmental benefit.

scholarly journals PSX-1 A newly developed pectinase from Aspergillus terreus enhanced feed utilization and milk production of Damascus goats fed pectin-rich diet

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 451-451
Author(s):  
Uchenna Anele ◽  
Hossam Azzaz ◽  
Ahmed Kholif ◽  
Hussein Murad ◽  
Nasr El-Bordeny ◽  
...  
Keyword(s):  
Milk Production ◽  
Production Efficiency ◽  
Aspergillus Terreus ◽  
Nutritive Value ◽  
Saturated Fatty Acids ◽  
Nutrient Digestibility ◽  
The Other ◽  
Atherogenic Index ◽  
Control Treatment ◽  
Lactating Goats

Abstract A newly developed pectinase from Aspergillus terreus was compared with a commercially available pectinase at 600 IU/kg feed. Thirty (one-week postpartum) Damascus goats stratified by previous milk production, body weight and parity were divided into three experimental treatments. Does were fed a basal diet containing concentrates, orange silage, sugar beet pulp and wheat straw at 50:20:20:10, respectively with (a newly developed pectinase or commercial pectinase) or without a supplement (control treatment). No difference (P &gt;0.05) was noted for feed intake but the newly developed pectinase increased (P &lt; 0.01) nutrient digestibility, diet nutritive value and milk production efficiency more than the other treatments. Out of all the blood parameters, only serum glucose was affected by the treatments with highest (P = 0.025) value noted for the new pectinase. Similarly, the new pectinase increased daily milk production (P &lt; 0.005) and the concentrations of milk components compared to the other two treatments. Additionally, pectinase (both the commercial and new) inclusion increased (P &lt; 0.05) the concentrations of total conjugated linoleic acid and unsaturated/saturated fatty acids ratio, and decreased atherogenic index (P = 0.01) compared with control treatment. It is concluded that the supplementation of the diet of lactating goats with pectinase at 600 IU/kg feed will enhance feed digestion and milk production. The newly developed pectinase performed better than the commercial pectinase.

scholarly journals Effects of garlic supplementation on in vitro nutrient digestibility, rumen fermentation, and gas production

2021 ◽  
Vol 51 (2) ◽  
pp. 271-279
Author(s):  
M.R. Kekana ◽  
D. Luseba ◽  
M.C. Muyu
Keyword(s):  
Fatty Acids ◽  
Crude Protein ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Garlic Powder

Garlic contains secondary metabolites with antimicrobial properties that can alter nutrient digestibility and rumen fermentation, similar to other antimicrobial products. The objectives of the study were to evaluate the effects of garlic powder and garlic juice on in vitro nutrient digestibility, rumen fermentation, and gas production. The treatments consisted of control with no additives, garlic powder, and garlic juice at 0.5 ml and 1 ml. The digestibility of dry matter, crude protein and neutral detergent fibre were determined after 48 hours incubation. Rumen ammonia nitrogen and volatile fatty acids were determined at 12 hours and 24 hours incubation. The cumulative gas production was recorded periodically over 48 hours. The in vitro dry matter disappearance decreased with 1 ml of garlic juice compared with control. The crude protein degradability in garlic powder and garlic juice was lower than in control. Volatile fatty acids increased in all treatments. Individual volatile fatty acids were significantly different, especially propionate, whereas the acetate to propionate ratio was reduced by garlic juice, and ammonia nitrogen was reduced by garlic powder and 0.5 ml of garlic juice. The cumulative gas production increased significantly with both levels of garlic juice. The addition of garlic juice at 0.5 mL/100 ml could enhance the production of propionate, and reduce the acetate to propionate ratio, implying that the supply of hydrogen for methanogens was limited.

Chlorella vulgaris microalgae and copper mixture supplementation enhanced the nutrient digestibility and milk attributes in lactating boer goats

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Ahmed E. Kholif ◽  
Ayman Y. Kassab ◽  
Hatem A. Hamdon
Keyword(s):  
Fatty Acids ◽  
Milk Production ◽  
Chlorella Vulgaris ◽  
Feed Efficiency ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Nutrient Digestibility ◽  
Atherogenic Index ◽  
Treatment Groups ◽  
Boer Goats

AbstractThirty-two lactating Boer goats (35.2 ± 1.4 kg body weight) were grouped into control and three treatment groups in completely randomised design. In treatment groups, supplementation was done as a mixture of 5 g Chlorella vulgaris + 4.5 mg of supplemental Cu/kg diet from CuSO4 (Alg5 treatment), 10 g C. vulgaris + 9 mg of supplemental Cu/kg diet (Alg10 treatment), or a mixture of 15 g C. vulgaris + 13.5 mg of supplemental Cu/kg diet (Alg15 treatment). Treatments did not affect feed intake; however, Alg10 treatment increased (P<0.001) nutrient digestibility. Treatments did not affect ruminal pH, ammonia-N, butyrate; however, the Alg10 treatment increased (P<0.01) ruminal total volatile fatty acids, propionate and acetate concentrations. Without affecting other blood measurements, the Alg10 treatment quadratically increased (P<0.001) serum glucose and Cu. The Alg10 treatments increased (P<0.001) daily milk production and the concentration of fat, and enhanced milk (feed) efficiency. The Alg10 treatment decreased (P<0.05) milk saturated fatty acids and the atherogenic index, and increased the proportions of total conjugated linoleic acids, C18:1n9t, odd fatty acids and total unsaturated fatty acids compared with the control treatment. Present study concluded that inclusion of a mixture of 10 g C. vulgaris + 9 mg Cu/kg diet in the diet of lactating Boer goats enhanced nutrient digestibility, ruminal fermentation, milk production, feed efficiency as well as milk nutritive value. Increasing the dose of the mixture to 15 g C. vulgaris + 13.5 mg Cu/kg diet is not recommended in the diet of lactating Boer goats.

scholarly journals A new pectinase produced from Aspergillus terreus compared with a commercial pectinase enhanced feed digestion, milk production and milk fatty acid profile of Damascus goats fed pectin-rich diet

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Hossam H. Azzaz ◽  
Ahmed E. Kholif ◽  
Hussein A. Murad ◽  
Nasr E. El-Bordeny ◽  
Hossam M. Ebeid ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Milk Production ◽  
Aspergillus Terreus ◽  
Nutrient Digestibility ◽  
Sugar Beet Pulp ◽  
The Other ◽  
Atherogenic Index ◽  
Control Treatment ◽  
Lactating Goats ◽  
Beet Pulp

AbstractPectinase hydrolyses pectin and increases the utilization of agriculture byproducts as feeds for ruminants. A newly developed pectinase from Aspergillus terreus was compared with a commercially available pectinase at 600 IU/kg feed on feed utilization and lactational performance of Damascus goat fed orange pulp and sugar beet pulp based diet (i.e., pectin-rich diet) for 12 weeks. Thirty (one week postpartum) Damascus goats stratified by previous milk production, body weight and parity were divided into three experimental groups. Does were fed a basal diet containing concentrates, orange silage, sugar beet pulp and wheat straw at 50:20:20:10, respectively without a supplement (control treatment) or supplemented with a newly developed pectinase (New treatment) or commercial pectinase (Commercial treatment). With similar (P>0.05) feed intake, the new pectinase increased (P<0.01) nutrient digestibility and milk production efficiency more than the other treatments. Out of all the blood parameters, only serum glucose was affected by the treatments with highest (P=0.025) value noted for the new pectinase. Similarly, the new pectinase increased daily milk production (P<0.005) and the concentrations of milk components compared to the other two treatments. Additionally, pectinase (both the commercial and new) inclusion increased (P<0.05) the concentrations of total conjugated linoleic acid and unsaturated/saturated fatty acids ratio, and decreased atherogenic index (P=0.01) compared with control treatment. It is concluded that the supplementation of the diet of lactating goats with pectinase at 600 IU/kg feed will enhance feed digestion and milk production. The newly developed pectinase performed better than the commercial pectinase.

scholarly journals Effects of garlic supplementation on in vitro nutrient digestibility, rumen fermentation and gas production.

2021 ◽  
Author(s):  
M. R. Kekana ◽  
D. Luseba ◽  
M. C. Muyu
Keyword(s):  
Fatty Acids ◽  
Crude Protein ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Garlic Powder

Abstract Garlic has secondary metabolites that possess antimicrobial properties that can alter nutrient digestibility and rumen fermentation similar to known antimicrobial products. The objectives of the study were to evaluate the effects of garlic powder and garlic juice on in vitro nutrient digestibility, rumen fermentation and gas production. The treatments were control no additives, garlic powder and garlic juice of 0.5 ml and 1 ml. The digestibility of dry matter, crude protein and neutral detergent fibre were determined after 48h incubation. Rumen ammonia nitrogen and volatile fatty acids were determined at 12h and 24h incubation. The cumulative gas production was recorded periodically over 48h. The in vitro dry matter disappearance decreased with 1 ml of garlic juice compared to control. The crude protein degradability in garlic powder and garlic juice was lower than in control. The volatile fatty acids increased in all the treatments. Individual volatile fatty acids were significantly different, especially propionate, while the acetate:propionate ratio was reduced by garlic juice, and ammonia nitrogen was reduced by garlic powder and 0.5 ml of garlic juice. The cumulative gas production increased significantly with both levels of garlic juice. The addition of garlic juice at 0.5 mL/100 mL could enhance the production of propionate, and reduce the acetate:propionate ratio implying that the supply of hydrogen for methanogens is limited.

scholarly journals Inclusion of rapeseed and pumpkin seed cakes in diets for Murciano-Granadina goats alters the fatty acid profile of milk

2021 ◽  
Vol 51 (2) ◽  
pp. 262-270
Author(s):  
I.M. Boldea ◽  
C. Dragomir ◽  
M.A. Gras ◽  
M. Ropotă
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Unsaturated Fatty Acids ◽  
Methyl Esters ◽  
Control Diet ◽  
Dietary Lipids ◽  
Italian Ryegrass ◽  
Pumpkin Seed ◽  
Lactating Goats

The objective of this research was to assess the effects of including oil-rich feedstuffs in diets for lactating goats on the fatty acid (FA) profile of their milk. Thirty-six Murciano-Granadina goats were randomly assigned to three treatment groups, namely a control diet (CTRL), a diet based on whole rapeseed (RS), and a diet based on pumpkin seed cake (PSC). The diets were composed of 1 kg hay (70 % Italian ryegrass, 30% alfalfa) and 1.24 kg concentrate, and were formulated to be isoenergetic and isonitrogenous. Milk yield and its contents of protein, fat and lactose did not differ significantly among the groups. However, including oil-rich feeds in the diet altered the fatty acid profile of the milk significantly, decreasing its saturated fatty acid (SFA) content and increasing its content of unsaturated fatty acids (UFAs). Effects on polyunsaturated fatty acids (PUFAs), conjugated linoleic acid (CLA), and the n-6 to n-3 ratio depended on the source of dietary lipids. The PSC augmented diet increased the relative amount of PUFAs and fatty acid methyl esters (FAME) in milk (+25 %) significantly In comparison with CTRL, whereas the RS diet produced a limited and statistically insignificant increase (+7.5%). The concentration of CLA was higher in milk from does fed the PSC diet, whereas the n-6 to n-3 ratio was lower in milk from does fed RS. These preliminary results form the basis for developing premium dairy products that are enriched in fatty acids that are more favourable for human health.

scholarly journals Using glycerin with chitosan extracted from shrimp residue to enhance rumen fermentation and feed use in native Thai bulls

2021 ◽  
pp. 1158-1164
Author(s):  
Anuthida Seankamsorn ◽  
Anusorn Cherdthong ◽  
Sarong So ◽  
Metha Wanapat
Keyword(s):  
Volatile Fatty Acids ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Nutrient Digestibility ◽  
Crude Glycerin ◽  
Ruminal Fermentation ◽  
Design Factor ◽  
Ch4 Production ◽  
High Level

Background and Aim: Crude glycerin is changed to propionate in the rumen, while chitosan can be used as a feed supplement to increase propionic acid concentration and decrease methane (CH4) production. We hypothesized that supplementation with a combination of a high level of crude glycerin with chitosan could have a beneficial effect on ruminal fermentation and mitigate CH4 production. This study aimed to explore the combined effects of crude glycerin and chitosan supplementation on nutrient digestibility, rumen fermentation, and CH4 calculation in native Thai bulls. Materials and Methods: Four 2-year-old native Thai bulls, weighing 150±20 kg, were kept in a 2×2 factorial arrangement in a 4×4 Latin square design. Factor A represented the incorporation of crude glycerin at 10.5% and 21% of the dry matter (DM) of a total mixed ration (TMR), and factor B represented the supplementation of chitosan at 1% and 2% DM of a TMR. Results: Increasing levels of crude glycerin at 21% decreased DM intake by 0.62 kg/day compared with 10.5% crude glycerin (p<0.05), whereas nutrient digestibility did not change (p>0.05). The incorporated crude glycerin and supplemented chitosan levels did not affect the pH, temperature, concentrations of ammonia-nitrogen, microbial population, and blood urea nitrogen (p>0.05). Supplemented chitosan and incorporated crude glycerin did not show any interaction effects on the molar portions and total volatile fatty acids (VFAs), except estimated CH4. Increasing the incorporated crude glycerin levels increased propionate and decreased the ratio of acetate to propionate ratio, whereas levels of butyrate, acetate, and total VFAs were unchanged. The combination of crude glycerin at 21% in the TMR with chitosan at 2% reduced CH4 estimation by 5.08% compared with the other feed treatment. Conclusion: Increasing incorporated crude glycerin levels in a TMR significantly elevated the propionate concentration, whereas combining 21% crude glycerin in the TMR diet with 2% chitosan supplementation could depress CH4 estimation more effectively than adding one of these supplements alone.

The effect of lipid sources on intake, rumen fermentation parameters and microbial protein synthesis in Nellore steers supplemented with glycerol

2014 ◽  
Vol 54 (10) ◽  
pp. 1871 ◽  
Author(s):  
Arturo Samuel Gomez Insuasti ◽  
Yury Tatiana Granja Salcedo ◽  
Pablo de Souza Castagnino ◽  
Bruno Ramalho Vieira ◽  
Euclides Braga Malheiros ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Ether Extract ◽  
Microbial Protein ◽  
Molar Proportion ◽  
Fermentation Parameters

The effects of glycerol with fat sources as a feed alternative were investigated in the ration for Nellore steers (Bos indicus). Eight cannulated steers at 30 months of age with initial bodyweight of 554 ± 36.0 kg were used in a double 4 by 4 Latin square design with four consecutive 16-day periods. Treatments were three different diets with lipid sources (soybean, soybean oil and calcium salts of fatty acids) and one diet control without lipid sources. All diets formulated contained 10% crude glycerol and 5% ether extract with a forage:concentrate ratio of 35:65; corn silage was used as forage. The ruminal liquid was sampled for 24 h and ruminal fermentations were monitored by measuring pH, concentrations of ammonia nitrogenand volatile fatty acids in rumen fluid. Urine samples were obtained from the total collection for 24 h for estimation of rumen microbial protein supply using urinary purine derivatives. Our results showed that the use of lipid sources combined with glycerol did not induce significant changes in rumen pH, acetate molar proportion, ruminal microbial protein or dry matter intake. Although the acetate molar proportion was kept constant within normal parameters, the propionate molar proportion was increased by the diet containing lipid sources. Moreover, we found that there was a negative effect of lipid sources on crude protein and neutral detergent fibre corrected for ash and protein intake, and one positive effect on ether extract intake. We believe that association between fat and glycerol may affect rumen fermentation parameters through reducing fibre intake and increasing propionate production and ammonia nitrogen.

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