scholarly journals Effects of Multi-Species Direct-Fed Microbial Products on Ruminal Metatranscriptome and Carboxyl-Metabolome of Beef Steers

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 72
Author(s):  
Megan McCoun ◽  
Adeoye Oyebade ◽  
Zaira M. Estrada-Reyes ◽  
Andres A. Pech-Cervantes ◽  
Ibukun M. Ogunade
Keyword(s):  
Fatty Acid ◽  
Amino Acid ◽  
Succinic Acid ◽  
Propionic Acid ◽  
Basal Diet ◽  
Beef Steers ◽  
Fermentation Products ◽  
Microbial Species ◽  
Amino Acid Degradation ◽  
Fatty Acid Peroxidation

We examined the effects of two direct-fed microbial (DFM) products containing multiple microbial species and their fermentation products on ruminal metatranscriptome and carboxyl-metabolome of beef steers. Nine ruminally-cannulated Holstein steers were assigned to 3 treatments arranged in a 3 × 3 Latin square design with three 21-d periods. Dietary treatments were (1) Control (CON; basal diet without additive), (2) Commence (PROB; basal diet plus 19 g/d of Commence), and (3) RX3 (SYNB; basal diet plus 28 g/d of RX3). Commence and RX3 are both S. cerevisiae-based DFM products containing several microbial species and their fermentation products. Mixed ruminal contents collected multiple times after feeding on day 21 were used for metatranscriptome and carboxyl-metabolome analysis. Partial least squares discriminant analysis revealed a distinct transcriptionally active taxonomy profiles between CON and each of the PROB and SYNB samples. Compared to CON, the steers fed supplemental PROB had 3 differential (LDA ≥ 2.0; p ≤ 0.05) transcriptionally active taxa, none of which were at the species level, and those fed SYNB had eight differential (LDA > 2.0, p ≤ 0.05) transcriptionally active taxa, but there was no difference (p > 0.05) between PROB and SYNB. No functional microbial genes were differentially expressed among the treatments. Compared with CON, 3 metabolites (hydroxylpropionic acid and 2 isomers of propionic acid) were increased (FC ≥ 1.2, FDR ≤ 0.05), whereas 15 metabolites, including succinic acid and fatty acid peroxidation and amino acid degradation products were reduced (FC ≤ 0.83, FDR ≤ 0.05) by supplemental PROB. Compared with CON, 2 metabolites (2 isomers of propionic acid) were increased (FC ≥ 1.2, FDR ≤ 0.05), whereas 2 metabolites (succinic acid and pimelate) were reduced (FC ≤ 0.83, FDR ≤ 0.05) by supplemental SYNB. Compared to SYNB, supplemental PROB reduced (FC ≤ 0.83, FDR ≤ 0.05) the relative abundance of four fatty acid peroxidation products in the rumen. This study demonstrated that dietary supplementation with either PROB or SYNB altered the ruminal fermentation pattern. In addition, supplemental PROB reduced concentrations of metabolic products of fatty acid peroxidation and amino acid degradation. Future studies are needed to evaluate the significance of these alterations to ruminal fatty acid and amino acid metabolisms, and their influence on beef cattle performance.

scholarly journals PSVI-3 Effects of dietary supplementation of multi-species direct-fed microbial products on energy status, apparent nutrient digestibility, and rumen metatranscriptome of beef steers

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 435-436
Author(s):  
Ibukun M Ogunade ◽  
Andres A Pech-Cervantes ◽  
Devan M Compart
Keyword(s):  
Bacillus Subtilis ◽  
Fatty Acid ◽  
Plasma Glucose ◽  
Basal Diet ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Beef Steers ◽  
Energy Status ◽  
Fermentation Products ◽  
Microbial Products

Abstract This study evaluated the effects of two different multi-strain direct-fed microbial products on energy status, nutrient digestibility, and ruminal metatranscriptome of beef steers. Nine rumen-cannulated Holstein steers were assigned to 3 treatments arranged in a 3 × 3 Latin square design with three 21-d periods. Dietary treatments were (1) CON (basal diet without additive), (2) PROB (basal diet plus 19 g/d of Commence), and (3) SYNB (basal diet plus 28 g/d of RX3). Commence is a blend of S. cerevisiae, Enterococcus lactis, Bacillus subtilis, Enterococcus faecium, and L. casei. RX3 is a blend of S. cerevisiae and the fermentation products of S. cerevisiae, Enterococcus lactis, Bacillus licheniformis, and Bacillus subtilis. Rumen fluid (for metatranscriptomics analysis) and blood samples (for analysis of plasma glucose and non-esterified fatty acid) were collected on d 21 of each period. From d 16 – 20, TMR and fecal samples were collected daily to determine apparent total tract digestibility of nutrients using indigestible neutral detergent fiber (iNDF) method. The data were analyzed using the GLIMMIX procedure of SAS. The model included the effects of treatment, period, and random effects of cow and square. There were no effects on DMI and non-esterified fatty acid. Compared with CON, steers fed either additives had greater (P = 0.02) plasma glucose concentrations. Results of metatranscriptome analysis revealed no differentially expressed functional genes among the treatments. Apparent total-tract digestibility of nutrients were also similar among treatments. These results demonstrated that supplemental PROB and SYNB improved the plasma glucose concentration, but had no effects on the functional capacity of the ruminal microbiome and apparent digestibility of nutrients in beef steers.

scholarly journals 396 Effect of zinc amino acid complexes on growth performance and carcass characteristics of finishing beef steers fed with or without ractopamine hydrochloride- a nine study pooled analysis

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 159-159
Author(s):  
Christopher Reinhardt ◽  
Allison Millican ◽  
Tryon Wickersham ◽  
Connie Larson ◽  
Mark Branine
Keyword(s):  
Amino Acid ◽  
Growth Performance ◽  
Basal Diet ◽  
Pooled Analysis ◽  
Feed Ratio ◽  
Experimental Unit ◽  
Beef Steers ◽  
Acid Complex ◽  
Main Effect ◽  
Ractopamine Hydrochloride

Abstract A nine-study retrospective pooled analysis was conducted to evaluate supplemental Zn provided as an amino acid complex (ZnAA) on growth performance, carcass quality and yield in finishing beef steers fed with or without ractopamine hydrochloride (RAC) prior to slaughter. Data from nine well-controlled studies conducted at university and commercial feedlot research facilities in AB, IA, KS, OK and TX were evaluated using 249 pens ranging from 6 to 275 steers /pen (Total number=14,096 animals.; initial BW = 340± 6.2 kg). Treatments evaluated were: (1) Control (CON) =basal diet with 0 to 11 mg-1·kg-1 DM ZnAA and no RAC; (2) RAC = 200 to 320 mg RAC ∙hd-1∙d-1 fed 28 to 42 d prior to slaughter; (3) ZnAA only =30 to 120 mg-1·kg-1 DM fed throughout finishing period; (4) ZnAA + RAC. Main effects of feeding RAC, ZnAA and RAC × ZnAA interaction were determined for growth performance and carcass data with pen as experimental unit and initial BW as covariate. Combined analyses indicated no RAC × ZnAA interactions (P ≥ 0.23). RAC main effect improved carcass-adjusted growth performance and increased HCW and ribeye area (P ≤ 0.01). Main effect ZnAA increased carcass-adjusted final BW, ADG, and HCW (P ≤ 0.01); improved carcass-adjusted Gain: DM feed ratio (P = 0.06), dressing percentage (P = 0.02), and increased calculated yield grade, backfat thickness and percentage of carcass internal fat (P ≤ 0.10). A RAC × ZnAA interaction (P ≤ 0.10) was observed for USDA quality and yield grade distribution of carcasses. Incidence and severity of liver abscesses were reduced (P ≤ 0.05) by feeding either ZnAA or RAC. Feeding ZnAA and RAC to finishing cattle separately or in combination were additive for producing incremental improvements in production and carcass traits of economic importance.

scholarly journals Comparative effects of two multispecies direct-fed microbial products on energy status, nutrient digestibility, and ruminal fermentation, bacterial community, and metabolome of beef steers

2020 ◽  
Vol 98 (9) ◽  
Author(s):  
Ibukun M Ogunade ◽  
Megan McCoun ◽  
Modoluwamu D Idowu ◽  
Sunday O Peters
Keyword(s):  
Bacterial Community ◽  
Relative Abundance ◽  
Plasma Glucose ◽  
Basal Diet ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Beef Steers ◽  
Ruminal Fermentation ◽  
Energy Status ◽  
Fermentation Products

Abstract We examined the effects of two direct-fed microbials (DFM) containing multiple microbial species and their fermentation products on energy status, nutrient digestibility, and ruminal fermentation, bacterial community, and metabolome of beef steers. Nine ruminally cannulated Holstein steers (mean ± SD body weight: 243 ± 12.4 kg) were assigned to three treatments arranged in a triplicated 3 × 3 Latin square design with three 21-d periods. Dietary treatments were 1) control (CON; basal diet), 2) Commence (PROB; basal diet plus 19 g/d of Commence), and 3) RX3 (SYNB; basal diet plus 28 g/d of RX3). Commence and RX3 are both multispecies DFM products. From day 16 to 20 of each period, feed and fecal samples were collected daily to determine the apparent total tract digestibilities of nutrients using indigestible neutral detergent fiber method. On day 21 of each period, blood samples were collected for analysis of plasma glucose and nonesterified fatty acid. Ruminal contents were collected at approximately 1, 3, 6, 9, 12, and 18 h after feeding on day 21 for analysis of volatile fatty acids (VFA), lactate, ammonia-N concentrations, bacterial community, and metabolome profile. Total tract digestibilities of nutrients did not differ (P > 0.05) among treatments. Compared with CON, steers fed either supplemental PROB or SYNB had greater (P = 0.04) plasma glucose concentrations. Compared with CON, total ruminal VFA, propionate, isovalerate, and valerate concentrations increased (P ≤ 0.05) or tended to increase (P ≤ 0.10) with either supplemental PROB or SYNB, but were not different (P > 0.05) between PROB and SYNB. Compared with CON, PROB reduced (P ≤ 0.05) the relative abundance of Prevotella 1 and Prevotellaceae UCG-001 but increased (P ≤ 0.05) the relative abundance of Rikenellaceae RC9, Succinivibrionaceae UCG-001, Succiniclasticum, and Ruminococcaceae UCG-002. Supplemental SYNB decreased (P ≤ 0.05) the relative abundance of Prevotella 1 and Prevotellaceae UCG-001 but increased (P ≤ 0.05) the relative abundance of Prevotella 7, Succinivibrio, Succiniclasticum, and Ruminococcaceae UCG-014. Compared with CON, metabolome analysis revealed that some amino acids were increased (P ≤ 0.05) in steers fed PROB. This study demonstrated that, compared with CON, supplementation of either PROB or SYNB altered the ruminal bacterial community and metabolome differently; however, their effects on the ruminal VFA profile and energy status of the steers were not different from each other.

scholarly journals PSII-6 Comparative effects of two multi-species direct-fed microbial products on rumen fermentation, bacterial community and metabolome of beef steers

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 398-398
Author(s):  
Ibukun M Ogunade ◽  
Devan M Compart ◽  
Andres A Pech-Cervantes
Keyword(s):  
Bacillus Subtilis ◽  
Bacterial Community ◽  
Relative Abundance ◽  
Isotope Labeling ◽  
Latin Square ◽  
Basal Diet ◽  
Beef Steers ◽  
Ruminal Fermentation ◽  
Metabolome Analysis ◽  
Fermentation Products

Abstract We examined the effects of two direct-fed microbials (DFM) containing multiple microbial species and their fermentation products on ruminal fermentation, bacterial community and metabolome of beef steers. Nine rumen-cannulated Holstein steers were assigned to 3 treatments arranged in a 3 × 3 Latin square design with three 21-d periods. Dietary treatments were (1) CON (basal diet without additive), (2) PROB (basal diet plus 19 g/d of Commence), and (3) SYNB (basal diet plus 28 g/d of RX3). Commence is a blend of active S. cerevisiae, Enterococcus lactis, Bacillus subtilis, Enterococcus faecium, and L. casei, and their fermentation products. RX3 is a blend of active S. cerevisiae and the fermentation products of S. cerevisiae, Enterococcus lactis, Bacillus licheniformis, and Bacillus subtilis. Daily dry matter intake was calculated. On d 21 of each period, ruminal contents were sampled at 3, 6, 12, and 18 h after feeding for analysis of VFA, bacterial community via 16S rRNA sequencing, and metabolome via chemical isotope labeling liquid chromatography mass spectrometry. There was no effect on DMI. Compared to CON, supplementation of either PROB or SYNB increased or tended to increase (P ≤ 0.10) total VFA, propionate, and valerate concentrations. Compared to CON, PROB reduced (P ≤ 0.05) the relative abundance of Prevotella 1 and Prevotellaceae UCG-001, but increased those of Rikenellaceae RC9, Succinivibrionaceae UCG-001, Succiniclasticum, Ruminococcaceae UCG-014, and Ruminococcaceae UCG-002, whereas SYNB decreased (P ≤ 0.05) the relative abundance of Prevotella 1 and Prevotellaceae UCG-001, and increased those of Prevotella 7, Succinivibrio, Succiniclasticum, and Ruminococcaceae UCG-014. Metabolome analysis revealed that 8 ruminal metabolites, including some amino acids, were increased (P ≤ 0.05) by PROB, whereas no differences were found for SYNB. This study demonstrated that supplemental PROB or SYNB altered the ruminal bacterial community and metabolome differently to achieve a similar ruminal fermentation pattern.

Maternal supplementation with uridine influences fatty acid and amino acid constituents of offspring in a sow–piglet model

2020 ◽  
pp. 1-14 ◽  
Author(s):  
Lu-min Gao ◽  
Yi-lin Liu ◽  
Xihong Zhou ◽  
Yan Zhang ◽  
Xin Wu ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Amino Acid ◽  
Excitatory Amino Acid ◽  
Basal Diet ◽  
Late Gestation ◽  
Hormone Sensitive Lipase ◽  
Amino Acid Transporters ◽  
Fatty Acid Elongase ◽  
Neonatal Piglets ◽  
Maternal Supplementation

Abstract To investigate the cumulative effects of maternal supplementation with nucleotides in the form of uridine (UR) on fatty acid and amino acid constituents of neonatal piglets, fifty-two sows in late gestation were assigned randomly into the control (CON) group (fed a basal diet) or UR group (fed a basal diet with 150 g/t UR). Samples of neonates were collected during farrowing. Results showed that supplementing with UR in sows’ diet significantly decreased the birth mortality of pigs (P = 0·05), and increased serum total cholesterol, HDL and LDL of neonatal piglets (P < 0·05). Moreover, the amino acid profile of serum and liver of neonatal piglets was affected by the addition of UR in sows’ diets (P < 0·05). Furthermore, an up-regulation of mRNA expression of energy metabolism-related genes, including fatty acid elongase 5, fatty acid desaturase 1, hormone-sensitive lipase and cholesterol-7a-hydroxylase, was observed in the liver of neonates from the UR group. Additionally, a decrease in placental gene expression of excitatory amino acid transporters 2, excitatory amino acid transporter 3 and neutral AA transporter 1 in the UR group was concurrently observed (P < 0·05), and higher protein expression of phosphorylated protein kinase B, raptor, PPARα and PPARγ in placenta from the UR group was also observed (P < 0·05). Together, these results showed that maternal UR supplementation could regulate placental nutrient transport, largely in response to an alteration of mTORC1–PPAR signalling, thus regulating the nutrition metabolism of neonatal piglets and improving reproductive performance.

Identification of the gene encoding 3-(5-oxo-2-thioxoimidazolidin-4-yl) propionic acid desulfhydrase in Burkholderia sp. HME13

2020 ◽  
Vol 85 (3) ◽  
pp. 626-629
Author(s):  
Hisashi Muramatsu ◽  
Hiroki Maguchi ◽  
Taisuke Harada ◽  
Takehiro Kashiwagi ◽  
Chul-Sa Kim ◽  
...  
Keyword(s):  
Amino Acid ◽  
Sequence Analysis ◽  
Amino Acid Sequence ◽  
Propionic Acid ◽  
Unknown Function ◽  
Protein Family ◽  
Amino Acid Sequence Analysis ◽  
Gene Encoding

ABSTRACT Here, we report the identification of the gene encoding a novel enzyme, 3-(5-oxo-2-thioxoimidazolidin-4-yl) propionic acid desulfhydrase, in Burkholderia sp. HME13. The enzyme converts 3-(5-oxo-2-thioxoimidazolidin-4-yl) propionic acid and H2O to 3-(2,5-dioxoimidazolidin-4-yl) propionic acid and H2S. Amino acid sequence analysis of the enzyme indicates that it belongs to the DUF917 protein family, which consists of proteins of unknown function.

scholarly journals Saccharomyces cerevisiae Fermentation of 28 Barley and 12 Oat Cultivars

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 59
Author(s):  
Timothy J. Tse ◽  
Daniel J. Wiens ◽  
Jianheng Shen ◽  
Aaron D. Beattie ◽  
Martin J. T. Reaney
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Lactic Acid ◽  
Succinic Acid ◽  
Alcoholic Fermentation ◽  
Ethanol Yield ◽  
Cereal Crops ◽  
Fermentation Products ◽  
Barley Cultivars ◽  
Oat Cultivars

As barley and oat production have recently increased in Canada, it has become prudent to investigate these cereal crops as potential feedstocks for alcoholic fermentation. Ethanol and other coproduct yields can vary substantially among fermented feedstocks, which currently consist primarily of wheat and corn. In this study, the liquified mash of milled grains from 28 barley (hulled and hull-less) and 12 oat cultivars were fermented with Saccharomyces cerevisiae to determine concentrations of fermentation products (ethanol, isopropanol, acetic acid, lactic acid, succinic acid, α-glycerylphosphorylcholine (α-GPC), and glycerol). On average, the fermentation of barley produced significantly higher amounts of ethanol, isopropanol, acetic acid, succinic acid, α-GPC, and glycerol than that of oats. The best performing barley cultivars were able to produce up to 78.48 g/L (CDC Clear) ethanol and 1.81 g/L α-GPC (CDC Cowboy). Furthermore, the presence of milled hulls did not impact ethanol yield amongst barley cultivars. Due to its superior ethanol yield compared to oats, barley is a suitable feedstock for ethanol production. In addition, the accumulation of α-GPC could add considerable value to the fermentation of these cereal crops.

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