scholarly journals PSVI-3 Effects of dietary supplementation of multi-species direct-fed microbial products on energy status, apparent nutrient digestibility, and rumen metatranscriptome of beef steers

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 435-436
Author(s):  
Ibukun M Ogunade ◽  
Andres A Pech-Cervantes ◽  
Devan M Compart
Keyword(s):  
Bacillus Subtilis ◽  
Fatty Acid ◽  
Plasma Glucose ◽  
Basal Diet ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Beef Steers ◽  
Energy Status ◽  
Fermentation Products ◽  
Microbial Products

Abstract This study evaluated the effects of two different multi-strain direct-fed microbial products on energy status, nutrient digestibility, and ruminal metatranscriptome of beef steers. Nine rumen-cannulated Holstein steers were assigned to 3 treatments arranged in a 3 × 3 Latin square design with three 21-d periods. Dietary treatments were (1) CON (basal diet without additive), (2) PROB (basal diet plus 19 g/d of Commence), and (3) SYNB (basal diet plus 28 g/d of RX3). Commence is a blend of S. cerevisiae, Enterococcus lactis, Bacillus subtilis, Enterococcus faecium, and L. casei. RX3 is a blend of S. cerevisiae and the fermentation products of S. cerevisiae, Enterococcus lactis, Bacillus licheniformis, and Bacillus subtilis. Rumen fluid (for metatranscriptomics analysis) and blood samples (for analysis of plasma glucose and non-esterified fatty acid) were collected on d 21 of each period. From d 16 – 20, TMR and fecal samples were collected daily to determine apparent total tract digestibility of nutrients using indigestible neutral detergent fiber (iNDF) method. The data were analyzed using the GLIMMIX procedure of SAS. The model included the effects of treatment, period, and random effects of cow and square. There were no effects on DMI and non-esterified fatty acid. Compared with CON, steers fed either additives had greater (P = 0.02) plasma glucose concentrations. Results of metatranscriptome analysis revealed no differentially expressed functional genes among the treatments. Apparent total-tract digestibility of nutrients were also similar among treatments. These results demonstrated that supplemental PROB and SYNB improved the plasma glucose concentration, but had no effects on the functional capacity of the ruminal microbiome and apparent digestibility of nutrients in beef steers.

scholarly journals Comparative effects of two multispecies direct-fed microbial products on energy status, nutrient digestibility, and ruminal fermentation, bacterial community, and metabolome of beef steers

2020 ◽  
Vol 98 (9) ◽  
Author(s):  
Ibukun M Ogunade ◽  
Megan McCoun ◽  
Modoluwamu D Idowu ◽  
Sunday O Peters
Keyword(s):  
Bacterial Community ◽  
Relative Abundance ◽  
Plasma Glucose ◽  
Basal Diet ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Beef Steers ◽  
Ruminal Fermentation ◽  
Energy Status ◽  
Fermentation Products

Abstract We examined the effects of two direct-fed microbials (DFM) containing multiple microbial species and their fermentation products on energy status, nutrient digestibility, and ruminal fermentation, bacterial community, and metabolome of beef steers. Nine ruminally cannulated Holstein steers (mean ± SD body weight: 243 ± 12.4 kg) were assigned to three treatments arranged in a triplicated 3 × 3 Latin square design with three 21-d periods. Dietary treatments were 1) control (CON; basal diet), 2) Commence (PROB; basal diet plus 19 g/d of Commence), and 3) RX3 (SYNB; basal diet plus 28 g/d of RX3). Commence and RX3 are both multispecies DFM products. From day 16 to 20 of each period, feed and fecal samples were collected daily to determine the apparent total tract digestibilities of nutrients using indigestible neutral detergent fiber method. On day 21 of each period, blood samples were collected for analysis of plasma glucose and nonesterified fatty acid. Ruminal contents were collected at approximately 1, 3, 6, 9, 12, and 18 h after feeding on day 21 for analysis of volatile fatty acids (VFA), lactate, ammonia-N concentrations, bacterial community, and metabolome profile. Total tract digestibilities of nutrients did not differ (P > 0.05) among treatments. Compared with CON, steers fed either supplemental PROB or SYNB had greater (P = 0.04) plasma glucose concentrations. Compared with CON, total ruminal VFA, propionate, isovalerate, and valerate concentrations increased (P ≤ 0.05) or tended to increase (P ≤ 0.10) with either supplemental PROB or SYNB, but were not different (P > 0.05) between PROB and SYNB. Compared with CON, PROB reduced (P ≤ 0.05) the relative abundance of Prevotella 1 and Prevotellaceae UCG-001 but increased (P ≤ 0.05) the relative abundance of Rikenellaceae RC9, Succinivibrionaceae UCG-001, Succiniclasticum, and Ruminococcaceae UCG-002. Supplemental SYNB decreased (P ≤ 0.05) the relative abundance of Prevotella 1 and Prevotellaceae UCG-001 but increased (P ≤ 0.05) the relative abundance of Prevotella 7, Succinivibrio, Succiniclasticum, and Ruminococcaceae UCG-014. Compared with CON, metabolome analysis revealed that some amino acids were increased (P ≤ 0.05) in steers fed PROB. This study demonstrated that, compared with CON, supplementation of either PROB or SYNB altered the ruminal bacterial community and metabolome differently; however, their effects on the ruminal VFA profile and energy status of the steers were not different from each other.

scholarly journals PSII-6 Comparative effects of two multi-species direct-fed microbial products on rumen fermentation, bacterial community and metabolome of beef steers

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 398-398
Author(s):  
Ibukun M Ogunade ◽  
Devan M Compart ◽  
Andres A Pech-Cervantes
Keyword(s):  
Bacillus Subtilis ◽  
Bacterial Community ◽  
Relative Abundance ◽  
Isotope Labeling ◽  
Latin Square ◽  
Basal Diet ◽  
Beef Steers ◽  
Ruminal Fermentation ◽  
Metabolome Analysis ◽  
Fermentation Products

Abstract We examined the effects of two direct-fed microbials (DFM) containing multiple microbial species and their fermentation products on ruminal fermentation, bacterial community and metabolome of beef steers. Nine rumen-cannulated Holstein steers were assigned to 3 treatments arranged in a 3 × 3 Latin square design with three 21-d periods. Dietary treatments were (1) CON (basal diet without additive), (2) PROB (basal diet plus 19 g/d of Commence), and (3) SYNB (basal diet plus 28 g/d of RX3). Commence is a blend of active S. cerevisiae, Enterococcus lactis, Bacillus subtilis, Enterococcus faecium, and L. casei, and their fermentation products. RX3 is a blend of active S. cerevisiae and the fermentation products of S. cerevisiae, Enterococcus lactis, Bacillus licheniformis, and Bacillus subtilis. Daily dry matter intake was calculated. On d 21 of each period, ruminal contents were sampled at 3, 6, 12, and 18 h after feeding for analysis of VFA, bacterial community via 16S rRNA sequencing, and metabolome via chemical isotope labeling liquid chromatography mass spectrometry. There was no effect on DMI. Compared to CON, supplementation of either PROB or SYNB increased or tended to increase (P ≤ 0.10) total VFA, propionate, and valerate concentrations. Compared to CON, PROB reduced (P ≤ 0.05) the relative abundance of Prevotella 1 and Prevotellaceae UCG-001, but increased those of Rikenellaceae RC9, Succinivibrionaceae UCG-001, Succiniclasticum, Ruminococcaceae UCG-014, and Ruminococcaceae UCG-002, whereas SYNB decreased (P ≤ 0.05) the relative abundance of Prevotella 1 and Prevotellaceae UCG-001, and increased those of Prevotella 7, Succinivibrio, Succiniclasticum, and Ruminococcaceae UCG-014. Metabolome analysis revealed that 8 ruminal metabolites, including some amino acids, were increased (P ≤ 0.05) by PROB, whereas no differences were found for SYNB. This study demonstrated that supplemental PROB or SYNB altered the ruminal bacterial community and metabolome differently to achieve a similar ruminal fermentation pattern.

scholarly journals Effects of Multi-Species Direct-Fed Microbial Products on Ruminal Metatranscriptome and Carboxyl-Metabolome of Beef Steers

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 72
Author(s):  
Megan McCoun ◽  
Adeoye Oyebade ◽  
Zaira M. Estrada-Reyes ◽  
Andres A. Pech-Cervantes ◽  
Ibukun M. Ogunade
Keyword(s):  
Fatty Acid ◽  
Amino Acid ◽  
Succinic Acid ◽  
Propionic Acid ◽  
Basal Diet ◽  
Beef Steers ◽  
Fermentation Products ◽  
Microbial Species ◽  
Amino Acid Degradation ◽  
Fatty Acid Peroxidation

We examined the effects of two direct-fed microbial (DFM) products containing multiple microbial species and their fermentation products on ruminal metatranscriptome and carboxyl-metabolome of beef steers. Nine ruminally-cannulated Holstein steers were assigned to 3 treatments arranged in a 3 × 3 Latin square design with three 21-d periods. Dietary treatments were (1) Control (CON; basal diet without additive), (2) Commence (PROB; basal diet plus 19 g/d of Commence), and (3) RX3 (SYNB; basal diet plus 28 g/d of RX3). Commence and RX3 are both S. cerevisiae-based DFM products containing several microbial species and their fermentation products. Mixed ruminal contents collected multiple times after feeding on day 21 were used for metatranscriptome and carboxyl-metabolome analysis. Partial least squares discriminant analysis revealed a distinct transcriptionally active taxonomy profiles between CON and each of the PROB and SYNB samples. Compared to CON, the steers fed supplemental PROB had 3 differential (LDA ≥ 2.0; p ≤ 0.05) transcriptionally active taxa, none of which were at the species level, and those fed SYNB had eight differential (LDA > 2.0, p ≤ 0.05) transcriptionally active taxa, but there was no difference (p > 0.05) between PROB and SYNB. No functional microbial genes were differentially expressed among the treatments. Compared with CON, 3 metabolites (hydroxylpropionic acid and 2 isomers of propionic acid) were increased (FC ≥ 1.2, FDR ≤ 0.05), whereas 15 metabolites, including succinic acid and fatty acid peroxidation and amino acid degradation products were reduced (FC ≤ 0.83, FDR ≤ 0.05) by supplemental PROB. Compared with CON, 2 metabolites (2 isomers of propionic acid) were increased (FC ≥ 1.2, FDR ≤ 0.05), whereas 2 metabolites (succinic acid and pimelate) were reduced (FC ≤ 0.83, FDR ≤ 0.05) by supplemental SYNB. Compared to SYNB, supplemental PROB reduced (FC ≤ 0.83, FDR ≤ 0.05) the relative abundance of four fatty acid peroxidation products in the rumen. This study demonstrated that dietary supplementation with either PROB or SYNB altered the ruminal fermentation pattern. In addition, supplemental PROB reduced concentrations of metabolic products of fatty acid peroxidation and amino acid degradation. Future studies are needed to evaluate the significance of these alterations to ruminal fatty acid and amino acid metabolisms, and their influence on beef cattle performance.

scholarly journals Effects of a blend of mannan and glucan on growth performance, apparent nutrient digestibility, energy status and whole-blood immune gene expression of beef steers during a 42-d receiving period

2020 ◽  
Author(s):  
Ibukun M Ogunade ◽  
Godstime Taiwo ◽  
Zaira M Estrada-Reyes ◽  
Y Jiang ◽  
Andres A Pech-Cervantes ◽  
...  
Keyword(s):  
Gene Expression ◽  
Plasma Glucose ◽  
Basal Diet ◽  
Immune Gene ◽  
Beef Steers ◽  
Energy Status ◽  
Metabolome Analysis ◽  
Blood Samples ◽  
Immune Gene Expression ◽  
Immune Related Genes

Abstract We examined the effects of dietary supplementation of a blend of mannan and glucan on the growth performance, energy status, and whole-blood immune gene expression of newly weaned beef steers during a 42 d receiving period. Forty eight newly weaned Angus crossbred steers (2 d post-weaning; 199 ± 13 kg of initial BW) from a single source were stratified by BW and randomly assigned to 1 of 2 treatments: basal diet with no additive (CON; n = 24) or a basal diet top-dressed with 5 g of a blend of mannan and glucan (MANGLU; n = 24). Average daily gain (ADG) and feed efficiency (FE) from days 1 – 14, 15 – 42, and 1 – 42 were calculated from daily dry matter intake (DMI) and weekly BW. Blood samples were collected on days 0, 14, and 42 for measurement of plasma glucose and NEFA. Blood samples collected on days 14 and 42 were composited for each steer for untargeted carbonyl-metabolome analysis (measurement of carbonyl-containing metabolites). Expression of 84 immune-related genes was analyzed on blood samples collected on day 42. Beginning on days 37 to 42, total mixed ration, refusals, and fecal samples were collected once daily to determine apparent total tract digestibility of DM, CP, NDF, and ADF using indigestible NDF as an internal marker. Over the 42-d feeding trial, supplemental MANGLU tended to increase final BW (P = 0.07) and ADG (P = 0.06). Compared to CON, beef steers fed supplemental MANGLU had greater (P = 0.01) DMI during the first 14 d, greater DM digestibility (P = 0.03), and tended to have greater NDF digestibility (P = 0.09). No treatment effects (P > 0.10) on plasma glucose and NEFA on d 14 and 42 were detected; however, carbonyl-metabolome analysis revealed increased (FDR ≤ 0.05) plasma concentrations of galactose and glyceraldehydes, and altered (FDR ≤ 0.05) concentrations of some microbiome-derived metabolites in beef steers fed MANGLU. Compared with CON, MANGLU increased (P ≤ 0.05) the expression of five immune-related genes involved in recognition of and mounting immune defense against microbial pathogens. In conclusion, the results of this study demonstrated that supplemental MANGLU enhances beef cattle immunocompetence and productivity during feedlot receiving period.

scholarly journals Nutritional value of high moisture corn silage in the diet of Holstein cows

2014 ◽  
Vol 66 (5) ◽  
pp. 1495-1503 ◽  
Author(s):  
P. Persichetti Júnior ◽  
G.A. Almeida Júnior ◽  
C. Costa ◽  
P.R.L. Meirelles ◽  
J.P.F. Silveira ◽  
...  
Keyword(s):  
Plasma Glucose ◽  
Latin Square ◽  
Nutrient Digestibility ◽  
Corn Silage ◽  
Neutral Detergent Fiber ◽  
Holstein Cows ◽  
High Moisture ◽  
Linear Effect ◽  
Five Phases ◽  
High Moisture Corn

Five Holstein cows were distributed in a 5x5 latin square design to assess the effect of replacement levels of dry ground corn grain (DGCG) by high moisture corn silage (HMCS) on intake, total nutrient digestibility and plasma glucose, according to the following treatments: 1) 100% DGCG; 2) 75% DGCG and 25% HMCS; 3) 50% DGCG and 50% HMCS; 4) 25% DGCG and 75% HMCS; 5) 100% HMCS. The experiment lasted 70 days, divided into five phases of 14 days each. The digestibility was obtained using chromic oxide (Cr2O3) as the indicator. Fecal samples were collected twice daily and blood samples were collected on the last day of each period before the first meal (0h) and 2h, 4h, 6h and 12h after the meal. There was no effect (P>0.05) on the intake of dry matter (DM), neutral detergent fiber (NDF), acid detergent fiber (ADF) and starch. The total apparent digestibility of DM, crude protein (CP), NDF and ADF were not affected (P>0.05) by the treatments, as well as the plasma glucose concentration. However, there was a decreased linear effect (P<0.05) for the protein intake and increased linear effect (P<0.05) for starch digestibility, as the level of HMCS was increased in the diets.

339 Effects of a Blend of Mannan and Glucan on Growth Performance and Immunocompetence of Newly Weaned Beef Steers

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 189-190
Author(s):  
Godstime A Taiwo ◽  
Ibukun M Ogunade
Keyword(s):  
Gene Expression ◽  
Growth Performance ◽  
Plasma Glucose ◽  
Basal Diet ◽  
Immune Defense ◽  
Immune Gene ◽  
Beef Steers ◽  
Blood Samples ◽  
Immune Gene Expression ◽  
Immune Related Genes

Abstract We examined the effects of dietary supplementation of a blend of mannan and glucan on the growth performance, diet digestibility, and whole-blood immune gene expression of newly weaned beef steers during a 42-d receiving period. Forty-eight newly weaned Angus crossbred steers (2 d post-weaning; 199 ± 13 kg of initial BW) were randomly assigned to 1 of 2 treatments: basal diet with no additive (CON; n = 24) or a basal diet top-dressed with 5 g of a blend of mannan and glucan (MANGLU; n = 24). Average daily gain (ADG) was calculated from weekly BW. Blood samples were collected on days 0, 14, and 42 for measurement of plasma glucose and NEFA. Expression of 84 immune-related genes was analyzed on blood samples collected on day 42. Beginning on days 37 to 42, total mixed ration, refusals, and fecal samples were collected once daily to determine apparent total tract digestibility of DM, CP, NDF, and ADF using indigestible NDF as an internal marker. Immune gene expression data was analyzed using a GeneGlobe Data Analysis Center. All other data were analyzed as a randomized complete block design using the GLIMMIX procedure of SAS. Over the 42-d feeding trial, MANGLU tended to increase final BW (P = 0.07) and ADG (P = 0.06). Compared to CON, beef steers fed supplemental MANGLU had greater DM digestibility (P = 0.03) and tended to have greater NDF digestibility (P = 0.09). No treatment effects (P &gt; 0.10) on plasma glucose and NEFA on d 14 and 42 were detected. Compared with CON, MANGLU increased (P ≤ 0.05) the expression of five immune-related genes involved in recognition of and mounting immune defense against microbial pathogens. In conclusion, this study demonstrated that supplemental MANGLU enhances beef cattle immunocompetence and productivity during feedlot receiving period.

scholarly journals Apparent total tract digestibility, ruminal fermentation, and blood metabolites in beef steers fed green-chopped cool-season forages

2020 ◽  
Vol 98 (6) ◽  
Author(s):  
Tessa M Schulmeister ◽  
Martin Ruiz-Moreno ◽  
Mariana E Garcia-Ascolani ◽  
Francine M Ciriaco ◽  
Darren D Henry ◽  
...  
Keyword(s):  
Lolium Multiflorum ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Blood Metabolites ◽  
Beef Steers ◽  
Annual Ryegrass ◽  
Ruminal Fermentation ◽  
Cool Season ◽  
Plasma Urea ◽  
Ruminal Ph

Abstract An experiment was conducted during the winter of two consecutive years to evaluate the effects of feeding green-chopped cool-season forages on digestibility, ruminal fermentation, and blood parameters in beef steers. Nine ruminally cannulated Angus crossbred steers (year 1: 359 ± 79 kg; year 2: 481 ± 105 kg) received ad libitum green-chopped forages from pastures planted with one of the following mixtures: 1) OAT = Horizon 201 oats (Avena sativa L.)/Prine annual ryegrass (Lolium multiflorum Lam.) at 95 and 17 kg/ha, respectively; 2) RYE = FL401 cereal rye (Secale cereale L.)/Prine annual ryegrass (Lolium multiflorum Lam.) at 78 and 17 kg/ha, respectively; or 3) TRIT = Trical 342 triticale (X Triticosecale spp.)/Prine annual ryegrass (Lolium multiflorum Lam.) at 95 and 17 kg/ha, respectively. Intake was measured using the GrowSafe system and orts were discarded prior to subsequent feeding. After a 14-d adaptation, feed and fecal samples were collected twice daily for 4 d to determine apparent total tract nutrient digestibility using indigestible neutral detergent fiber (NDF) as an internal marker. On day 19, blood and ruminal fluid samples were collected every 3 h during a 24-h period to analyze plasma urea nitrogen (PUN) and glucose, ruminal pH, and concentration of ruminal ammonia nitrogen (NH3-N) and volatile fatty acids (VFA). Data were analyzed as a generalized randomized block design with repeated measures using the PROC MIX of SAS. No effect of treatment (P &gt; 0.05) was observed for intake of dry matter, organic matter (OM), crude protein, NDF, or acid detergent fiber. Apparent total tract digestibility of nutrients was greater (P &lt; 0.05) for OAT and TRIT when compared with RYE, with OM digestibility being 82.7%, 79.6%, and 69.5%, respectively. An effect of time (P &lt; 0.01) was observed for ruminal pH. Plasma concentration of glucose was greater (P &lt; 0.01) in steers consuming OAT, whereas steers fed RYE had greater (P &lt; 0.05) concentrations of ruminal NH3-N and PUN, and the least concentration of total ruminal VFA (P &lt; 0.05), despite having the greatest (P &gt; 0.05) molar proportion of acetate, branched-chain VFA, and acetate:propionate. Increased nutrient digestibility and favorable ruminal fermentation and blood metabolites of OAT and TRIT are potentially conducive to enhanced growth performance when compared with RYE.

Effects of processing and fat content of coarse canola screenings on voluntary intake and total tract nutrient digestibility of beef steers

2000 ◽  
Vol 80 (1) ◽  
pp. 153-159 ◽  
Author(s):  
S. Pylot ◽  
J. J. McKinnon ◽  
A. F. Mustafa ◽  
V. J. Racz ◽  
D. A. Christensen
Keyword(s):  
Dietary Fat ◽  
Protein Digestibility ◽  
Nutrient Utilization ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Beef Steers ◽  
Dry Matter Digestibility ◽  
Fiber Digestibility ◽  
Fat Level ◽  
Voluntary Intake

The effects of feeding canola screenings on voluntary intake (VI) and nutrient digestibility by growing steers were determined in two experiments. Twelve steers were involved in the first experiment to study the effects of processing (grinding and pelleting) of canola screenings on VI and apparent total tract dry matter digestibility (DMD), crude protein digestibility (CPD), fatty acid digestibility (FAD), neutral detergent fiber digestibility (NDFD), and acid detergent fiber digestibility (ADFD). In the second experiment, the effects of fat level from canola screenings on VI and total tract nutrient digestibilities were determined using 12 steers. The fat levels tested were 67, 100, 128, and 162 g kg−1 ether extract (EE). Feeding processed canola screenings (176 g kg−1 EE) reduced (P < 0.05) VI by 31% and improved (P < 0.05) DMD, CPD, and FAD by 18, 19, and 28%, respectively. Processing of canola screenings reduced (P < 0.05) ADFD by 15% with no effect on NDFD. Increasing dietary fat level from 67 to 162 g kg−1 reduced VI in a quadratic manner (P < 0.05) and improved NDFD in a linear (P < 0.05) fashion. However, DMD, CPD, and ADFD were not affected by fat level in the diet. Total tract digestibility of fatty acids decreased linearly (P < 0.05) from 822 to 640 g kg−1 with increasing dietary fat level. The results of the study indicate that dietary fat from canola screenings at levels up to 100 g kg−1 can be fed to growing steers without detrimental effects on feed intake. Key words: Canola screenings, fat, intake, nutrient utilization, steers.

scholarly journals COMPLETE RUMEN MODIFIER SUPPLEMENTATION IN CORN COB SILAGE BASAL DIET OF LAMB REDUCES METHANE EMISSION

2017 ◽  
Vol 18 (1) ◽  
pp. 33
Author(s):  
Dwi Yulistiani ◽  
Wisri Puastuti ◽  
Budi Haryanto ◽  
Agung Purnomoadi ◽  
M. Kurihara ◽  
...  
Keyword(s):  
Methane Emission ◽  
Methane Production ◽  
Basal Diet ◽  
Rumen Fermentation ◽  
Nutrient Digestibility ◽  
The Body ◽  
Neutral Detergent Fiber ◽  
Feed Consumption ◽  
Feed Additive ◽  
Corn Cob

<p>Feeding animal with fibrous materials such as corn cob will emit methane. Complete rumen modifier (CRM) is an improved feed additive comprised a mixture of Sapindus rarak, sesbania, albizia leaves and minerals that functions as a methane inhibitor. The study aimed to determine the effect of CRM supplementation on the feed intake, nutrient digestibility, rumen fermentation, methane emission and growth of lambs. The experiment was designed in a complete randomized block, four levels of CRM (0%, 1%, 2%, and 3%), six group of 24 male lambs per treatment based on the body weight.  Basal diet used was corn cob silage ad libitum and concentrate (500 g/day) as a supplement. The results showed that CRM supplementation did not affect feed consumption and average daily gain, but significantly decreased the dry matter, as well as organic matter and protein digestibility.  The neutral detergent fiber (NDF) and acid detergent fiber (ADF) digestibility linearly decreased with increasing level of CRM. Ruminal pH, ammonia concentration and volatile fatty acid (VFA) concentration were not affected by the CRM supplementation. Methane production expressed in kJ/MJ gross energy (GE) or digestible energy (DE) intake significantly decreased by 32% at the 2–3% CRM supplementation and reduced by 39% when methane production was expressed in g/kg digested NDF. It can be concluded that 2% CRM supplementation in the corn cob basal diet did not affect nutrient intake and growth rate of the lamb, as well as rumen fermentation.  The study suggests that CRM is an environmentally friendly feed additive for lamb</p>

scholarly journals Intake and nutrient digestibility of tropical grasses and legume hay supplemented with tilapia fish silage

1969 ◽  
Vol 92 (1-2) ◽  
pp. 27-38
Author(s):  
Héctor L. Díaz-Ríos ◽  
Abner A. Rodríguez-Carias
Keyword(s):  
Crude Protein ◽  
Latin Square ◽  
Basal Diet ◽  
Protein Digestibility ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Arachis Glabrata ◽  
Fiber Digestibility ◽  
Digitaria Eriantha ◽  
Voluntary Intake

Two experiments were conducted to determine the effect of supplementation with tilapia fish silage (FS) prepared in laboratory scale silos with addition of cane molasses, on intake and digestibility of tropical grass hay (GH) (Digitaria eriantha, 80%, and Urochloa maximum, 20%), and rhizome perennial peanut hay (RPPH) (Arachis glabrata). In Experiment I, GH was the basal diet and treatments were Control or T1 = GH, and GH supplemented daily with FS at 0.45% (T2) and 0.90% (T3) of the animal body weight (BW). In Experiment II, the basal diet was RPPH and treatments were Control or T1 = RPPH;T2 = RPPH supplemented daily with FS at 0.225% BW; and T3 = RPPH with 0.45% BW. In both experiments nine adult intact rams were used. The animals were fed for eight days of adaptation and six days of data collection in each period. Both experiments used a 3 X 3 Latin Square design. In experiment I dry matter (DM) intake and digestibility of GH increased (P less than 0.05) with FS supplementation, which also increased crude protein (CP) intake. CP digestibility increased (P < 0.05) at each level of supplementation compared to that of the control, but T2 and T3 did not differ significantly. Neutral detergent fiber (NDF) intake was higher (P < 0.05) in T1 than in T3 but similar in T1 and T2. Fiber digestibility was not affected (P greater than 0.05) by the treatments. It was concluded that supplementation of a GH basal diet with FS at 0.90% of BW is the best alternative. In Experiment II, DM digestibility of T3 differed (P less than 0.05) from that in T1 but in T2 did not differ from DM digestibility either in T1 or T3. Intake of DM, CP and fiber were similar in all treatments (P greater than 0.05). Crude protein digestibility was similar at both levels of supplementation and superior (P < 0.05) to that of the control, but fiber digestibility was not affected (P > 0.05). It is concluded that daily supplementation with FS for sheep fed a basal diet of RPPH should be limited to 0.225% of BW. Upon comparing both experiments, voluntary intake of DM, NDF and CP from RPPH was higher than from GH.The legume also showed a higher (P < 0.05) digestibility of DM and CP.

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