scholarly journals The Expression of Selected Wnt Pathway Members (FZD6, AXIN2 and β-Catenin) in Canine Oral Squamous Cell Carcinoma and Acanthomatous Ameloblastoma

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1615
Author(s):  
Barbora Putnová ◽  
Iveta Putnová ◽  
Miša Škorič ◽  
Marcela Buchtová
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Wnt Pathway ◽  
Human Cancer ◽  
Expression Patterns ◽  
Wnt Signaling Pathway ◽  
Biological Behavior ◽  
Cytokeratin Expression

The Wnt signaling pathway is well known to be involved in many types of human cancer; however, in veterinary medicine, the investigation of individual Wnt members’ expression, and their role in or association with oral tumor pathogenesis, is still underevaluated. We aim to determine the expression pattern of Frizzled-6 (FZD6) as one of the Wnt receptors in two of the most common canine oral neoplastic lesions—canine oral squamous cell carcinoma (COSCC) and canine acanthomatous ameloblastoma (CAA). While COSCC is a malignant tumor with aggressive biological behavior and a tendency to metastasize, CAA is a benign tumor with high local invasiveness. In CAA, the expression of FZD6 was mostly located in the center of the epithelial tumorous tissue, and cells exhibiting features of squamous metaplasia were strongly positive. In well-differentiated COSCC, FZD6 was expressed in the tumorous epithelium as well as the tumorous stroma. There was a negative correlation between cytokeratin expression and FZD6 expression in COSCC, where the central parts of the epithelial tumorous tissue were often FZD6-negative. The non-differentiated COSCC with low expression of cytokeratin exhibited a diffuse FZD6 signal. The invasive front with areas of tumor budding exhibited high FZD6 expression with a loss of cytokeratin expression. Moreover, the expression of β-catenin and AXIN2 was increased in comparison to gingiva. In conclusion, our study revealed significant differences in the expression patterns and the levels of FZD6 between COSCC and CAA, indicating the differential engagement of the Wnt pathway in these tumors.

Relationship Between mir15b and Sal-Like Protein 4 and Biological Behavior of Oral Squamous Cell Carcinoma

2021 ◽  
Vol 11 (2) ◽  
pp. 308-314
Author(s):  
Zengbo Wu ◽  
Yan Yan ◽  
Xianzhuo Chen ◽  
Yanling Liu ◽  
Dinggen Chen
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Normal Mucosa ◽  
Clinicopathological Features ◽  
Biological Behavior ◽  
Control Group ◽  
Tumor Tissues ◽  
Proliferation And Invasion

miR15b and SALL4 are involved in a variety of tumor progression. The roles of miR15b and SALL4 in oral squamous cell carcinoma (OSCC) remains unclear. The tumors and normal mucosa of OSCC patients were collected to detect miR15b and SALL4 level by Real-time PCR and analyze their correlation with OSCC clinicopathological features. Oral cancer Tca8113 cells were separated into control group; miR15b mimics group and miR15b inhibitor group followed by analysis of SALL4 expression, cell survival by MTT assay; cell invasion by Transwell chamber assay, as well as expression of N-cadherin and Vimentin and correlated with TNM stage, tumor volume and metastasis, and positively with differentiation TGF-β by Western blot. miR15b expression was decreased and SALL4 expression was increased in OSCC tumor tissues. miR15b was negatively degree (P < 0.05), whereas, opposite correlation of SALL4 with the above parameters was found (P < 0.05). miR15b and SALL4 were negatively correlated. MiR15b mimics significantly up-regulated MiR15b, decreased SALL4 expression, inhibited Tca8113 cell proliferation and invasion, as well as reduced N-cadherin, Vimentin and TGF-βexpression (P < 0.05). Opposite results were found in MiR15b inhibitor group. MiR15b expression is decreased and SALL 4 is increased in OSCC tumor tissues. MiR15b and SALL4 is closely related to OSCC clinicopathological features. MiR15b regulates the expression of EMT-related genes and TGF-β, thereby altering the proliferation and invasion of OSCC cells.

scholarly journals miR-222 regulates the cell biological behavior of oral squamous cell carcinoma by targeting PUMA

2014 ◽  
Vol 31 (3) ◽  
pp. 1255-1262 ◽  
Author(s):  
FANGFANG JIANG ◽  
WEI ZHAO ◽  
LIJIE ZHOU ◽  
LIN ZHANG ◽  
ZIFENG LIU ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Biological Behavior

scholarly journals Roles of the Wnt Signaling Pathway in Head and Neck Squamous Cell Carcinoma

2021 ◽  
Vol 7 ◽  
Author(s):  
Jing Xie ◽  
Li Huang ◽  
You-Guang Lu ◽  
Da-Li Zheng
Keyword(s):  
Squamous Cell Carcinoma ◽  
Wnt Signaling ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Signaling Pathway ◽  
Squamous Cell ◽  
Wnt Pathway ◽  
Wnt Signaling Pathway ◽  
Neck Squamous Cell Carcinoma ◽  
Upstream Gene

Head and neck squamous cell carcinoma (HNSCC) is the most common type of head and neck tumor. It is a high incidence malignant tumor associated with a low survival rate and limited treatment options. Accumulating conclusions indicate that the Wnt signaling pathway plays a vital role in the pathobiological process of HNSCC. The canonical Wnt/β-catenin signaling pathway affects a variety of cellular progression, enabling tumor cells to maintain and further promote the immature stem-like phenotype, proliferate, prolong survival, and gain invasiveness. Genomic studies of head and neck tumors have shown that although β-catenin is not frequently mutated in HNSCC, its activity is not inhibited by mutations in upstream gene encoding β-catenin, NOTCH1, FAT1, and AJUBA. Genetic defects affect the components of the Wnt pathway in oral squamous cell carcinoma (OSCC) and the epigenetic mechanisms that regulate inhibitors of the Wnt pathway. This paper aims to summarize the groundbreaking discoveries and recent advances involving the Wnt signaling pathway and highlight the relevance of this pathway in head and neck squamous cell cancer, which will help provide new insights into improving the treatment of human HNSCC by interfering with the transcriptional signaling of Wnt.

scholarly journals Elucidation of β-Catenin Cyclin D1 Pathway in Oral Squamous Cell Carcinoma

2018 ◽  
Vol 2 (2) ◽  
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cyclin D1 ◽  
Squamous Cell ◽  
Cell Cycle Regulation ◽  
Mrna Level ◽  
Wnt Signaling Pathway ◽  
Cycle Regulation

Background: Oral cancer is sixth most common cancer in India with poor overall disease free survival. In last decade major changes in the cancer management has happened but no such advantage has been seen in the survival of oral cancer patients. One major reason for the poor survival of head and neck squamous cell carcinoma (HNSCC) is lack of good predictive and prognostic biomarkers. Different studies have shown that in cancer cells, cell-cycle regulatory protein expression is altered. Cyclin D1 is a key regulatory molecule in cell cycle regulation. Many of the molecular alterations that cause abnormal biologic behaviour of cancer cells are based on aberrations of cell cycle regulation. Studies have demonstrated that Cyclin D1, c-Myc and MMP7 were important target genes of WNT signaling pathway and overexpression of them was highly associated with accumulation of β-Catenin and mutational defects of the WNT signaling pathway in numerous tumor types. Aim: This study was planned to characterize the β-Catenin and Cyclin D1 transcript level expression pattern in oral squamous cell carcinoma (OSCC) samples. Materials and Methods: Expression patterns of β-Catenin and Cyclin D1 were studied in OSCC at the transcript and protein levels by using qRT-PCR and immunohistochemistry (IHC) respectively. χ2, t-tests and ANOVA were used for the statistical analyses. Results: β-Catenin and Cyclin D1 were significantly overexpressed in oral squamous cell carcinoma cases when compared to normal. Correlation regression analysis showed the expression of Cyclin D1 and β-Catenin at mRNA level were positively correlated. Further, in immunohistochemical analysis β-Catenin showed cytoplasmic staining rather than nuclear. Conclusion: It is concluded that β-Catenin and Cyclin D1 mRNA level analysis using Real-time PCR could serve as biomarkers in oral squamous cell carcinoma since their expression is consistently altered in majority of the oral squamous cell carcinoma samples.

scholarly journals Immunoexpression of P16 and Ki 67 in oral squamous cell carcinoma: Association with clinicopathological parameters.

2020 ◽  
Vol 3 (2) ◽  
pp. 135-144
Author(s):  
Shadan Omer ◽  
Payman Rashid
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Biological Behavior ◽  
Clinicopathological Parameters ◽  
Statistical Association ◽  
Ki 67 ◽  
Lower Lip ◽  
Significant Statistical Association

Background and Objectives: Oral squamous cell carcinoma (OSCC) is considered as a major health problem worldwide and has been associated with high recurrence rate and poor progno-sis. Advances in understanding of OSCC have not improved the outcome in their management significantly. Many studies have focused on the roles of biomolecular markers in OSCC. The use of p16 and Ki67 as biomarkers of biological behavior of oral squamous cell carcinoma is contro-versial. This study aimed to determine immunoexpression of P16 and Ki67 in oral squamous cell carcinoma and to evaluate their association with various clinicopathological parameters. Materials and Methods: Fifty cases of squamous cell carcinoma from different locations in the oral cavity were included in this cross sectional study. The cases were collected from Rizgary Teaching Hospital and Private Laboratories in Erbil city during a period of eight months from October 2018 to May 2019. The expression of p16 and Ki 67 were evaluated immunohistochem-ically; the findings were correlated with the age of the patients, gender, site of the tumor and grade of the tumor. Result: A total of 50 patients with oral squamous cell carcinoma were enrolled in this study the age ranged from 33 to 89 years, with a mean age ± SD of (64.24 ±12.01) years and more than half (52.0%) of them were males. Lower lip was the most common site of the tumor followed by upper lip and tongue (42.0%, 26.0% and 18.0%, respectively). Histopathological findings of the tumor showed that (54.0%) of the patients had moderately differentiated squamous cell carci-noma. However, (84.0%) of the patients showed negative expression of P 16, while Ki 67 ex-pression was positive among (76.0%) of them. No significant statistical association were found between immunoexpression of p16 and age, sex of patient, site of the tumor and grade of the tumor (P=0.67, P=0,095, P=0.696, P=0.454 respectively). No significant statistical association were found between immunoexpression of Ki67 and age, sex of patient, site of the tumor and grade of the tumor (P=0.637, P=0,411, P=0.353, P=1.00 respectively). Conclusion: in relation to the results obtained in this study no significant association were found between P16 and Ki 67 immunoexpression in oral squamous cell carcinoma with clinicopatho-logical parameters. Further researches have to be designed to better understand the role of p16 and Ki 67 in OSCC. Keywords: oral squamous cell carcinoma, immunoexpression, P16, Ki67.

scholarly journals Tumor-associated tissue eosinophilia in oral squamous cell carcinoma – A predictable biological behavior

2020 ◽  
Vol 3 ◽  
pp. 3-8
Author(s):  
Parikshya Shrestha ◽  
Keerthi Narayan ◽  
Varsha Vimal Kumar ◽  
Girish C. Hemadala ◽  
Sanjay Murgod
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Eosinophil Count ◽  
Malignant Neoplasm ◽  
Biological Behavior ◽  
Eosinophil Infiltration ◽  
Tissue Eosinophilia ◽  
Special Stain

Objectives: Squamous cell carcinoma of the oral cavity is the most common aggressive epithelial malignant neoplasm. Its biological behavior is influenced by the host immune cells, such as multifaceted eosinophils, associated with wound healing and tissue damage processes. Their presence within a variety of human cancers raises queries about their role. The infiltrations of tumor stroma by eosinophils are believed to play a significant role in progression of the carcinoma and could be either a potential diagnostic tool for stromal invasion or as a prognostic indicator. Its role in cancer still remains unclear since in the literature, there are very few studies showing improved prognosis and few contradictory studies showing poor prognosis. This study was conducted with an aim to compare the tumor-associated tissue eosinophilia in oral squamous cell carcinoma (OSCC) and normal tissue and to correlate the expression in different grades of carcinoma using a special stain that targets eosinophils exclusively and vividly. Materials and Methods: The study includes 30 samples, six normal, and 24 histopathologically diagnosed with OSCC. 5 μ thick sections were made and stained using special stain and examined under high power (×40), ten consecutive microscopic fields were studied. The average numbers of eosinophils were statistically analyzed. Results: Eosinophil count for carcinoma was higher compared to normal mucosa, but the comparison in different grades of cancer did not show much difference. Conclusion: Since eosinophil count was higher in carcinoma, eosinophil infiltration in dysplastic cases should prompt thorough evaluation for invasiveness.

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