scholarly journals Bayesian Analysis of the Effects of Olive Oil-Derived Antioxidants on Cryopreserved Buck Sperm Parameters

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2032
Author(s):  
Ander Arando Arbulu ◽  
Francisco Javier Navas González ◽  
Alejandra Bermúdez-Oria ◽  
Juan Vicente Delgado Bermejo ◽  
África Fernández-Prior ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Bayesian Inference ◽  
Bayesian Analysis ◽  
Protective Effect ◽  
Olive Oil ◽  
Sperm Motility ◽  
Mitochondrial Potential ◽  
Low Concentrations ◽  
Acrosome Integrity ◽  
Positive Effect

The present study evaluates the effect of olive oil-derived antioxidants, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), on cryopreserved caprine sperm using Bayesian inference of ANOVA. For this proposal, sperm was collected, pooled and diluted in freezing media supplemented with different concentrations of HT, DHPG and the mixture (MIX) of both antioxidants. Sperm motility, viability, acrosome integrity, mitochondrial status, and lipid peroxidation (LPO) were assessed in fresh and frozen-thawed sperm samples. The results provided evidence that HT at low concentrations improves sperm motility and viability, and reduces the LPO. Contrastingly, DHPG and MIX exert a positive effect by reducing LPO values as concentration increases. Additionally, mitochondrial potential was reduced when samples were supplemented with HT at low concentrations and mixture of both antioxidants. Conclusively, the addition of olive oil-derived antioxidants (HT at 10 µg/mL and DHPG at 30 µg/mL) implements a protective effect in cryopreserved buck sperm. Bayesian analysis alternatives offer new possibilities to determine the repercussion of antioxidants on sperm, both quantitatively and qualitatively.

Effect of docosahexanoic acid on quality of frozen–thawed bull semen in BioXcell extender

2017 ◽  
Vol 29 (3) ◽  
pp. 490 ◽  
Author(s):  
Asmatullah Kaka ◽  
Wahid Haron ◽  
Rosnina Yusoff ◽  
Nurhusien Yimer ◽  
A. M. Khumran ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Membrane Integrity ◽  
Optimum Level ◽  
Docosahexanoic Acid ◽  
Normal Morphology ◽  
Bull Semen ◽  
Acrosome Integrity

This study was conducted to investigate the effect of docosahexanoic acid (DHA) supplementation in BioXcell extender on the quality of frozen–thawed bull semen. Twenty-four ejaculates were collected from three bulls (eight from each bull). Ejaculates with motility ≥70% and normal morphology ≥80% were extended into BioXcell extender to which 0 (control), 3, 5, 10 or 15 ng mL–1 DHA was added. The supplemented semen samples were incubated at 37°C for 15 min for DHA uptake by spermatozoa. Later, samples were cooled for 2 h at 5°C and packaged into 0.25-mL straws, frozen in liquid nitrogen for 24 h and subsequently thawed for evaluation. Results are presented as percentages ± s.e.m. Supplementation with DHA at 3 ng mL–1 significantly improved sperm functional parameters including sperm motility, normal morphology, viability, acrosome integrity and membrane integrity when compared with other supplemented groups and the control. Lipid peroxidation increased as the incorporation of DHA supplementation increased. In conclusion, 3 ng mL–1 concentration of DHA resulted in superior quality of frozen–thawed bull spermatozoa and is suggested as the optimum level of DHA to be added into BioXcell extender.

315 REACTIVE OXYGEN SPECIES AND OXIDATIVE STRESS IN CANINE SEMEN FRACTIONS

2010 ◽  
Vol 22 (1) ◽  
pp. 313
Author(s):  
C. F. Lucio ◽  
M. Nichi ◽  
F. M. Regazzi ◽  
T. F. Rück ◽  
L. C. G. Silva ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Free Radicals ◽  
Sperm Motility ◽  
Seminal Plasma ◽  
Pearson Correlation ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Low Concentrations ◽  
Viable Sperm

Reactive oxygen species (ROS) are physiologically produced by spermatozoa and leukocytes present in the seminal plasma. In low concentrations, ROS play an important role in sperm function because they are required for sperm fertilizing capacity, mainly during sperm capacitation and hyperactivation (Saleh RA and Agarwal A 2002 J. Androl. 23, 737-752). However, an imbalance between the formation of free radicals and the capacity for defense of the antioxidant mechanisms may lead to cell damage (Rover Jr L et al. 2001 Química Nova 24, 112-119). Spermatozoa are sensitive to lipid peroxidation due to its high content of polyunsaturated fatty acids and low concentrations of protective enzymes (Sharma RK and Agarwa LA 1996 Urology 48, 835-850). The aims of the present study were to compare ROS content among the 3 fractions of canine semen and to correlate these values with sperm variables. Semen samples were collected from 15 healthy dogs of distinct breeds aged 2 to 6 years. Sperm analysis was performed through motility, forward progressive velocity, morphology, and the percentage of viable sperm with the use of the eosin/nigrosin stain. The determination of thiobarbituric acid reactive substances (TBARS) was used to estimate the degree of lipid peroxidation in each sperm fraction. Values were compared using ANOVA and Tukey’s test for multiple comparisons at a significance level of 5%. Pearson correlation was used to calculate the relationship between sperm variables in the second fraction. Sperm motility, velocity, and percentage of viable sperm were within the normal range for canine semen: 84±2%, 3.4±0.1%, and 83 ± 2%, respectively. The sperm-rich fraction presented statistically higher concentrations of TBARS (1474.19 ± 245.78 ng mL-1) compared to the first and third fractions (579.41 ± 171.23 and 399.62 ± 58.08 ng mL-1, respectively; P < 0.05), indicating that spermatozoa and epididymal secretions are the main source of free radicals. No statistical correlation among TBARS and sperm motility and velocity were verified. However, a positive correlation was observed between the percentage of sperm proximal droplets and TBARS (r = 0.44, P = 0.7). This result suggests that a high incidence of sperm proximal droplets can enhance ROS formation in seminal plasma. Hence, canine sperm presenting delayed maturation in the epididymes produce higher concentrations of free radicals. In fact, sperm production of ROS occurs mainly by abnormal cells, especially the ones containing cytoplasm residues (Gomez E et al. 1998 Int. J. Androl. 21, 81-94). However, no protective effect of the sperm distal droplets was verified in canine semen as observed elsewhere for the bovine spermatozoa (Nichi M et al. 2007 Theriogenology 67, 334-340). In conclusion, spermatozoa and epididymal fluids are the primordial source of free radicals in canine seminal plasma, mainly when sperm proximal droplets are present.

scholarly journals The Effect of L-Carnitine, Hypotaurine, and Taurine Supplementation on the Quality of Cryopreserved Chicken Semen

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Agnieszka Partyka ◽  
Olga Rodak ◽  
Joanna Bajzert ◽  
Joanna Kochan ◽  
Wojciech Niżański
Keyword(s):  
Lipid Peroxidation ◽  
Flow Cytometry ◽  
Sperm Motility ◽  
Control Group ◽  
Taurine Supplementation ◽  
Mitochondrial Potential ◽  
Membrane Reorganization ◽  
Viable Sperm ◽  
Live Sperm

The objective of this study was to investigate the effect of L-carnitine (LC), hypotaurine (HT), and taurine (T) on the quality of frozen-thawed chicken semen. Pooled semen samples were divided into seven aliquots (control, 1 mM LC, 5 mM LC, 1 mM HT, 10 mM HT, 1 mM T, and 10 mM T) and subjected to cryopreservation. Postthaw sperm motility was determined by IVOS system and sperm characteristics were assessed with fluorochromes and flow cytometry. The highest sperm motility and the highest percentage of viable sperm were in the HT1 group (P<0.01andP<0.05) following cryopreservation. After thawing, we observed a higher percentage of sperm without apoptosis and membrane reorganization changes in the LC1 and T1 group when compared to the control (P<0.05). There was a higher percentage of live sperm without lipid peroxidation (LPO) in all treatments (P<0.01;P<0.05), when compared to the control group. The percentage of sperm with high mitochondrial potential significantly increased with LC1, T1, and T10 (P<0.05). Supplementation of the diluent with LC1, LC5, and T1 significantly (P<0.05) reduced DNA susceptibility to fragmentation, compared to the control and HT1 groups. These results indicate that the addition of examined antioxidants improves the quality of cryopreserved chicken semen.

scholarly journals Effect of semen extender supplementation with sericin on post-thaw dairy bull sperm quality and lipid peroxidation

2021 ◽  
Vol 66 (No. 1) ◽  
pp. 13-20
Author(s):  
Yotsapon Yangnam ◽  
Suttiwat Chapanya ◽  
Thevin Vongpralub ◽  
Wuttigrai Boonkum ◽  
Vibuntita Chankitisakul
Keyword(s):  
Lipid Peroxidation ◽  
Sperm Motility ◽  
Mammalian Cells ◽  
Semen Quality ◽  
Sperm Quality ◽  
Protective Role ◽  
Holstein Friesian ◽  
Acrosome Integrity ◽  
Bull Sperm ◽  
Dairy Bull

Silk sericin plays a protective role in a variety of mammalian cells during cryopreservation. This study aimed to determine the effects of sericin supplementation to a freezing extender on sperm quality and lipid peroxidation in dairy bull sperm cryopreservation. Each semen sample of five Holstein-Friesian crossbred bulls was divided into four aliquots and diluted in a tris-egg yolk extender supplemented with different concentrations of sericin [0%, 0.25%, 0.5%, and 1.0% (wt/vol)]. Sperm motility (CASA), viability, acrosome integrity, mitochondrial membrane potential (fluorescent staining) and lipid peroxidation (malondialdehyde – MDA test) were analysed. The results show that the 0.25% and 0.5% sericin groups had the highest total sperm motility (P &lt; 0.05). Sperm viability, acrosome integrity, and mitochondrial function were higher in the group supplemented with 0.25% sericin compared to the control and 1.0% (P &lt; 0.05). Sericin supplementation with 0.25% and 0.5% significantly decreased MDA concentrations compared with the control (P &lt; 0.05). In conclusion, supplementation of the semen freezing extender with sericin at the concentration of 0.25% significantly improved the post-thaw semen quality and reduced lipid peroxidation in Holstein-Friesian crossbred bulls.

Protective effect of simple phenols from extravirgin olive oil against lipid peroxidation in intestinal Caco-2 cells

2010 ◽  
Vol 48 (10) ◽  
pp. 3008-3016 ◽  
Author(s):  
Monica Deiana ◽  
Giulia Corona ◽  
Alessandra Incani ◽  
Debora Loru ◽  
Antonella Rosa ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Protective Effect ◽  
Olive Oil

scholarly journals Short term administration of cyproterone acetate for contraception: Effects on testosterone secretion and semen characteristics in rams (Ovis aries) and bucks (Capra hircus)

2021 ◽  
Vol 18 (4) ◽  
pp. e0407
Author(s):  
Viviana N. Flores-Gil ◽  
Rosario Velázquez ◽  
Adolfo Toledano-Díaz ◽  
Julián Santiago-Moreno ◽  
Antonio López-Sebastián
Keyword(s):  
Olive Oil ◽  
Sperm Motility ◽  
Cyproterone Acetate ◽  
Ovis Aries ◽  
Short Term ◽  
Morphological Abnormalities ◽  
Testosterone Secretion ◽  
Progressive Motility ◽  
Semen Characteristics ◽  
Acrosome Integrity

Aim of study: To examine the influence of administering cyproterone acetate (CPA), at the beginning of the mating season, on the testosterone concentration and morphometric and functional characteristics of ram and buck semen.Area of study: Madrid, SpainMaterial and methods: Five rams and five bucks were intramuscularly administered 200 mg of CPA in 2 mL of olive oil twice per week - from July 1st to 31st in the rams, and from August 1st to 31st in the bucks. Five control animals of each species were administered 2 mL of olive oil. Blood samples and ejaculates analysed from the start of treatment until eight weeks after the last day of treatment.Main results: GLM-ANOVA showed the interaction species × CPA treatment to have effect (p<0.05) on sperm motility, progressive motility and acrosome integrity; and greater effect (p<0.01) on curvilinear velocity (VCL), straight-line velocity (VSL), viability, and morphological abnormalities. In both the rams and bucks, plasma testosterone levels fell from the first week from the start of CPA administration until three weeks after the end of treatment. In rams, the total sperm count, sperm motility, progressive motility, viability, morphological abnormalities, VCL and VSL were all negatively affected by the treatment (p<0.001); acrosome integrity was also affected (p<0.05). In bucks, sperm motility, progressive motility, VCL, VSL and morphological abnormalities were negatively affected (p<0.05).Research highlights: Treatment with CPA affected testosterone secretion, semen characteristics and sperm morphometry in both the rams and bucks, and thus it might be used as short term contraceptive protocol in small ruminants.

scholarly journals Effect of Pseudomonas moorei KB4 Cells’ Immobilisation on Their Degradation Potential and Tolerance towards Paracetamol

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 820
Author(s):  
Robert Surma ◽  
Danuta Wojcieszyńska ◽  
Jagna Karcz ◽  
Urszula Guzik
Keyword(s):  
Protective Effect ◽  
Kinetic Parameters ◽  
Toxicity Assessment ◽  
Bacterial Cells ◽  
Low Concentrations ◽  
Toxicity Analysis ◽  
Immobilised Cells ◽  
Degradation Activity ◽  
High Concentrations ◽  
The Hill

Pseudomonas moorei KB4 is capable of degrading paracetamol, but high concentrations of this drug may cause an accumulation of toxic metabolites. It is known that immobilisation can have a protective effect on bacterial cells; therefore, the toxicity and degradation rate of paracetamol by the immobilised strain KB4 were assessed. Strain KB4 was immobilised on a plant sponge. A toxicity assessment was performed by measuring the concentration of ATP using the colony-forming unit (CFU) method. The kinetic parameters of paracetamol degradation were estimated using the Hill equation. Toxicity analysis showed a protective effect of the carrier at low concentrations of paracetamol. Moreover, a pronounced phenomenon of hormesis was observed in the immobilised systems. The obtained kinetic parameters and the course of the kinetic curves clearly indicate a decrease in the degradation activity of cells after their immobilisation. There was a delay in degradation in the systems with free cells without glucose and immobilised cells with glucose. However, it was demonstrated that the immobilised systems can degrade at least ten succeeding cycles of 20 mg/L paracetamol degradation. The obtained results indicate that the immobilised strain may become a useful tool in the process of paracetamol degradation.

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