scholarly journals Preliminary Test of the Reduction Capacity for the Intestinal Adsorption of Skatole and Indole in Weaning Piglets by Pure and Coated Charcoal

Animals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2720
Author(s):  
Franziska Witte ◽  
Aleksandar Pajic ◽  
Florian Menger ◽  
Igor Tomasevic ◽  
Dana Carina Schubert ◽  
...  
Keyword(s):  
Small Intestine ◽  
Large Intestine ◽  
High Capacity ◽  
Preliminary Test ◽  
Boar Taint ◽  
Intact Male ◽  
Body Weights ◽  
Weaning Piglets ◽  
Reduction Capacity

To reduce the risk of boar taint, intact male piglets are immuno- or surgically castrated. One alternative is reducing skatole by adding skatole reducing or adsorbing substances to the boars’ diet. Charcoal with a high capacity for adsorbing skatole and indole in vitro (tested before, data not shown) was fed to the boars to test the hypothesis that a fat coating prevents the unspecific adsorption of charcoal before entry into the large intestine while increasing skatole adsorption. Twelve male and six female weaning piglets with initial body weights of 7.74 ± 0.75 kg were fed for 18 (or 19) days with either 2% pure (untreated) charcoal or 4% coated (50% charcoal + 50% fat-coating) charcoal or no charcoal. After euthanasia, skatole and indole were quantified in caecum and colon chyme. Skatole and indole contents in caecum chyme were significantly lower (p < 0.05) in the group fed with coated charcoal (33 ± 4.2, 7 ± 2.8 µg/gDM, respectively) than in the group fed with pure charcoal (51 ± 7.3, 14 ± 3.0 µg/gDM) or with no charcoal (73 ± 12.6, 15 ± 1.7 µg/gDM). Similar effects were obvious for colon chyme. The results indicate that a fat coating of charcoal might prevent unspecific adsorption in the small intestine and might consequently lead to a higher adsorption capacity for skatole and indole in the large intestine, as skatole and indole concentrations in the chyme of caecum and colon were approximately 50% lower in the piglets who received coated charcoal.

scholarly journals Effect of diet on disaccharidase activity in the chick

1972 ◽  
Vol 27 (2) ◽  
pp. 343-352 ◽  
Author(s):  
R. C. Siddons
Keyword(s):  
Small Intestine ◽  
Large Intestine ◽  
Marked Decrease ◽  
Specific Effect ◽  
Dietary Carbohydrate ◽  
Sucrase Activity ◽  
Disaccharidase Activity ◽  
Maltase Activity ◽  
Body Weights ◽  
Protein Free Diet

1. The influence of diets, particularly dietary carbohydrate, on the development of the intestinal disaccharidases of the chick was studied.2. The maltase activity in the small intestine was similar in groups of 25-d-old chicks that had been fed, from hatching, on diets containing either starch, glucose, maltose, sucrose, or a mixture of 50% glucose + 50% lactose, as the source of carbohydrate. The sucrase activity in the small intestine was also similar in the different groups, as was the palatinase (the enzyme that hydrolyses palatinose, i.e. 6-o-α-D-glucopyranosyl-D-fructose) activity. The maltase activity in the large intestine of the group receiving the starch-containing diet was significantly increased. The lactase activity in the large intestine was significantly higher in the group receiving the 50% glucose + 50% lactose and in the group receiving glucose than in the other groups. Body- weights were similar with all the diets.3. The fasting of chicks for a period of 3 d caused a marked decrease in the activity of the disaccharidases and in the protein content of the homogenates of the small intestine.4. The maltase activity was similar in the small intestine of chicks that had been fasted for 3 d and subsequently given diets containing either starch, glucose, maltose, sucrose or fructose for 5 d. The sucrase activity, the isomaltase activity and the palatinase activity were also similar in the small intestine of the chicks given the different diets. Feeding with a fat-free or protein-free diet did not affect the development of disaccharidases in the small intestine, but feeding with a carbohydrate-free diet resulted in reduced disaccharidase activity.5. The results suggest that, in the chick, dietary carbohydrate is necessary for the development of the disaccharidases but the form of the carbohydrate is not important. None of the sugars tested had a specific effect on a particular disaccharidase.

Evaluation of moist heat treatment of canola meal on digestion in the rumen, small intestine, large intestine and total digestive tract of steers

1995 ◽  
Vol 75 (3) ◽  
pp. 279-283 ◽  
Author(s):  
S. A. Moshtaghi Nia ◽  
J. R. Ingalls
Keyword(s):  
Heat Treatment ◽  
Small Intestine ◽  
Digestive Tract ◽  
Large Intestine ◽  
Canola Meal ◽  
Glucosinolate Content ◽  
Moist Heat ◽  
Rumen Degradation ◽  
Heat Treated

The effect of heat treatment on canola meal (CM) dry matter (DM) and nitrogen (N) degradability in the rumen and digestion in the small and large intestines and total digestive tract of steers was determined. CM was moist-heat-treated at 127 °C for 15 and 45 min. The degradation of DM and N was estimated using small nylon bags in the rumen for 0.1, 4, 8, 12, 16, 24, 48 and 72 h. The mobile bag technique with an in vitro incubation in an acid-pepsin solution was used with a separate set of bags to estimate the digestion of each nutrient in the small and large intestines as well as the total digestive tract for rumen incubation times of 8, 16 and 24 h. Chemical analysis of heated CM showed a significant reduction in soluble N, sucrose and glucosinolate content while the concentration of ADIN increased. Treatment significantly reduced the degradation of DM and N in the rumen while the digestibility of DM and N in the small intestine increased with both treatments proportional to the decreased rumen degradation. Heat treatment had no effect on DM and N digestibility at the large intestine site. Total tract disappearance of DM and N were not affected by 15 min heating; however, after 45 min heating, DM and N disappearances were reduced. Moist heat treatment was effective in reducing rumen disappearance of CM nitrogen while it increased the N digestion in the small intestine. Key words: Heat treatment, canola meal, ruminant intestine, protein digestion

Kinetics of hexose uptake by the small and large intestine of the chicken

1996 ◽  
Vol 271 (4) ◽  
pp. R1085-R1089 ◽  
Author(s):  
C. Amat ◽  
J. M. Planas ◽  
M. Moreto
Keyword(s):  
Large Intestine ◽  
Diffusion Constant ◽  
High Capacity ◽  
Minor Role ◽  
Passive Mechanism ◽  
Hexose Uptake ◽  
Kinetics Of ◽  
Distal Direction ◽  
Small And Large Intestine

The kinetic parameters of hexose uptake by the small and large intestine of the chicken have been determined in vitro. Rates of initial influx of alpha-methyl-D-glucoside and L-glucose were measured in everted sleeves of the duodenum, jejunum, ileum, proximal cecum, and rectum. Results show the following. 1) Maximal transport capacity values for alpha-methyl-D-glucoside show that the jejunum is the segment that is best suited for Na(+)-mediated uptake. 2) The calculated apparent Michaelis constant values were (in mmol/l) 11.6 for duodenum, 7.8 for jejunum, 3.5 for ileum, 2.4 for proximal cecum, and 7.1 for rectum. This suggests that, with the exception of the rectum, the affinity of the carrier for alpha-methyl-D-glucoside progressively increases in the distal direction. 3) Diffusion constant values indicate that influx of hexoses by a passive mechanism in the duodenum and proximal cecum is significantly higher than in the other segments. 4) The sum of passive and mediated mechanisms confers to the duodenum and jejunum a high capacity to absorb hexoses. The ileum, proximal cecum, and rectum have a quantitatively minor role, albeit significant, in completing the absorptive function.

scholarly journals Broiler chicken body weights, feed intakes, plasma lipid and small-intestinal bile acid concentrations in response to feeding of chitosan and pectin

1997 ◽  
Vol 78 (2) ◽  
pp. 283-291 ◽  
Author(s):  
A. Razdan ◽  
D. Pettersson ◽  
J. Pettersson
Keyword(s):  
Small Intestine ◽  
Bile Acid ◽  
Broiler Chicken ◽  
Plasma Cholesterol ◽  
Control Diet ◽  
Plasma Lipid ◽  
Cholesterol Ratio ◽  
Body Weights

One-day-old broiler chickens were fed on a control diet based on maize and maize starch or diets containing 30g/kg of 89 % deacetylated chitin (chitosan) or low-methoxyl (34 % degree of esterification) pectin. Feeding of the chitosan diet to chickens significantly reduced body weights and feed intakes compared with animals fed on control or pectin diets on days 5 and 11 of the experiment. On day 12, significant reductions in total plasma cholesterol and HDL-cholesterol concentrations were observed among birds fed on the chitosan but not the pectin diet in relation to control-fed animals. A concomitant increase in the plasma HDL-cholesterol:total cholesterol ratio was observed among chitosan-fed chickens. The generally reduced concentrations of primary and total bile acids in the duodenum of birds fed on the fibre-containing diets on day 13 may have been an indication of a delay in the production and/or secretion of bile. Viscosity of the three broiler-chicken diets was measured after suspension in water, acidification and finally neutralization of the suspensions, in an attempt to simulate the effect of changes in pH and dilution of diets occurring in the gizzard and small intestine of chickens. Viscosity of the chitosan diet was significantly elevated after acidification and significantly reduced at neutralization in comparison with the control and pectin-containingdiets suggesting that the hypolipidaemic influence of chitosan observed in the present study may be due to interruption of enterohepatic bile acid circulation rather than increased viscosity in the small intestine of chickens. The low viscosity of the pectin dietin vitrotogether with the absence of a hypocholesterolaemic effect of this diet when fedin vivoprecludes any conclusion regarding the hypocholesterolaemic mechanism of pectin observed in earlier studies.

scholarly journals Potential Rates of Fermentation in Digesta from the Gastrointestinal Tract of Pigs: Effect of Feeding Fermented Liquid Feed

2003 ◽  
Vol 69 (1) ◽  
pp. 408-418 ◽  
Author(s):  
Ole Højberg ◽  
Nuria Canibe ◽  
Bettina Knudsen ◽  
Bent Borg Jensen
Keyword(s):  
Small Intestine ◽  
Lactic Acid ◽  
Gastrointestinal Tract ◽  
Large Intestine ◽  
Distal Part ◽  
Standing Stock ◽  
Short Chain ◽  
Coliform Bacteria ◽  
Liquid Feed

ABSTRACT Microbial catabolic capacity in digesta from the gastrointestinal tract of pigs fed either dry feed or fermented liquid feed (FLF) was determined with the PhenePlate multisubstrate system. The in vitro technique was modified to analyze the kinetics of substrate catabolism mediated by the standing stock of enzymes (potential rates of fermentation), allowing a quantitative evaluation of the dietary effect on the catabolic capacity of the microbiota. In total, the potential rates of fermentation were significantly reduced in digesta from the large intestine (cecum, P < 0.1; colon, P < 0.01; and rectum, P < 0.0001) of pigs fed FLF compared to pigs fed dry feed. No effect of diet was observed in the stomach (P = 0.71) or the distal part of the small intestine (P = 0.97). The highest rates of fermentation and the most significant effect of diet were observed for readily fermentable carbohydrates like maltose, sucrose, and lactose. Feeding FLF to pigs also led to a reduction in the large intestine of the total counts of anaerobic bacteria in general and lactic acid bacteria specifically, as well as of microbial activity, as determined by the concentration of ATP and short-chain fatty acids. The low-molecular-weight carbohydrates were fermented mainly to lactic acid in the FLF before being fed to the animals. This may have limited microbial nutrient availability in the digesta reaching the large intestine of pigs fed FLF and may have caused the observed reduction in activity and density of the cecal and colonic microbial population. On the other hand, feeding FLF to pigs reduced the viable counts of coliform bacteria (indicator of Escherichia coli and Salmonella spp.) most profoundly in the stomach and the distal part of the small intestine, probably due to the bactericidal effect of lactic acid and low pH. The results presented clearly demonstrate that feeding FLF to pigs had a great impact on the indigenous microbiota, as reflected in bacterial numbers, short-chain fatty acid concentration, and substrate utilization. However, completely different mechanisms may be involved in the proximal and the distal parts of the gastrointestinal tract. The present study illustrates the utility of the PhenePlate system for quantifying the catabolic capacity of the indigenous gastrointestinal tract microbiota.

scholarly journals Effect of previous nutrition on body composition and maintenance energy costs of growing lambs

1986 ◽  
Vol 56 (3) ◽  
pp. 595-605 ◽  
Author(s):  
C. L. Ferrell ◽  
L. J. Koong ◽  
J. A. Nienaber
Keyword(s):  
Body Composition ◽  
Body Weight ◽  
Small Intestine ◽  
Large Intestine ◽  
Heat Production ◽  
Intact Male ◽  
Period 2 ◽  
Similar Weight ◽  
Free Body ◽  
Fasting Heat Production

1. Forty-eight intact male lambs (30 kg) were fed to gain 16 (H), 5 (M) or –6 (L) kg during a 42 d interval (period 1). Lambs from each of the H and M groups were fed to gain either 16 (HH, MH), 5 (HM, MM) or –6 (HL, ML) kg and lambs from the L group were fed to gain 27 (LS), 16 (LH) or 5 (LM) kg during the ensuing 42 d (period 2).2. Fasting heat production (FHP) of four lambs from each treatment was determined at the end of period 2.3. Weights and compositions of the carcass, offal and digesta-free body as well as weights of major internal organs were determined for four lambs of each treatment at the end of periods 1 and 2.4. Within groups of lambs of similar weight at the end of period 2, body composition was, in general, similar, but FHP was greater in lambs that had been on higher planes of nutrition during period 2.5. Within groups of lambs of similar weight, lambs that were fed at higher planes of nutrition during period 2 had greater weights or proportions of liver, small intestine, large intestine and stomach.6. Neither weight of the liver, kidney, stomach, small intestine, large intestine nor daily fasting heat production were constant functions of body-weight. Relations of these traits to body-weight changed with rate of gain.7. Regression analysis indicated that the feeding of lambs at higher planes of nutrition during period 1 resulted in higher maintenance requirements of those lambs during period 2.

Electron probe x-ray microanalysis and electron microscopy of amino acid absorption and transport by the small intestine: inhibition by chemical poisons and correlation to the elemental composition of the epithelial cells

1986 ◽  
Vol 44 ◽  
pp. 134-135
Author(s):  
A. J. Tousimis
Keyword(s):  
Small Intestine ◽  
Amino Acid ◽  
Epithelial Cells ◽  
Elemental Composition ◽  
Isotope Labeling ◽  
Structural Components ◽  
X Ray ◽  
Human Small Intestine ◽  
Transport Studies

The elemental composition of amino acids is similar to that of the major structural components of the epithelial cells of the small intestine and other tissues. Therefore, their subcellular localization and concentration measurements are not possible by x-ray microanalysis. Radioactive isotope labeling: I131-tyrosine, Se75-methionine and S35-methionine have been successfully employed in numerous absorption and transport studies. The latter two have been utilized both in vitro and vivo, with similar results in the hamster and human small intestine. Non-radioactive Selenomethionine, since its absorption/transport behavior is assumed to be the same as that of Se75- methionine and S75-methionine could serve as a compound tracer for this amino acid.

Anatomi Saluran Pencernaan biawak air (Varanus salvator) (Reptil: Varanidae)

2019 ◽  
Author(s):  
Mahfud Mahfud ◽  
Ihwan
Keyword(s):  
Small Intestine ◽  
Digestive Tract ◽  
Large Intestine ◽  
Scientific Information ◽  
Tissue Perfusion ◽  
Animal Population ◽  
Varanus Salvator ◽  
Commercial Purpose ◽  
Left And Right ◽  
Biology Laboratory

Excessive hunting and poaching for commercial purpose of Varanus salvator in Indonesia can cause a decline in this animal population. However, the scientific information of this animal especially about the biologic of organ system is rarely reported. Therefore, this case opens up opportunities for researching, which aims to study the anatomy of digestive tract of water monitor macroscopically. This research has been conducted in Biology Laboratory, University of Muhammadiyah Kupang for 5 months from March to August 2016. The digestive organ of this animal that has been preserved in alcohol 70% was obtained before from two males of water monitors. Preservation process: the animal were anesthetized, exsanguinated, and fixated in 4 paraformaldehyde by tissue perfusion method. Observations were performed to the visceral site and morphometrical of digestive tract. The resulted data was analysed descriptively and presented in tables and figures. The digestive tract of water monitor consist of esophagus, stomach, small intestine, large intestine and cloaca. The dimension of each organ is different based on its structures and functions. The esophagus of water monitor connects the mouth cavity and the stomach and also as the entrance of food to the stomach. Water monitor stomach were found in cranial part of abdomen, in left side of liver. The small intestine was longer than stomach and it is a winding muscular tube in abdomen in posterior side of liver. The large intestine consist of colon and cloaca, while cecum was not found. This channel was extend lateromedially in abdomen to cloaca between left and right kidneys. The cloaca was the end of digestive tract which excreted feces and urine. From this research, we can conclude that the digestive tract of water monitor consists of esophagus, stomach, small intestine, and large intestine. It’s difficult to differentiate small intestine and large intestine because there are no cecum.

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