scholarly journals Impact of Storage Conditions on Equine Fecal Inoculum for Estimating In Vitro Digestibility

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3195
Author(s):  
Delaney O’Donnell ◽  
Lacy Sukovaty ◽  
Gary Webb
Keyword(s):  
Regression Model ◽  
Storage Condition ◽  
Storage Conditions ◽  
In Vitro Digestibility ◽  
Fecal Material ◽  
Ols Regression ◽  
Quarter Horse

This study evaluated the effect of storage conditions of equine fecal material on the viability of microbial inoculum used for in vitro equine digestibility trials. Pooled fecal material from three mature Quarter Horse geldings was stored at 39 °C anaerobically for 15 min (control), while aerobic samples were stored at 22 °C for 6 h (SC1), 3 °C for 6 h (SC2), and −18 °C for 24 h (SC3). Following storage, the feces were utilized to prepare microbial inoculum for the digestion of six different forages using the Daisy II Incubator. After incubation, DM, NDF, and ADF compositions were determined and used to calculate DMD, NDFD, and ADFD. Analysis using the OLS regression model for differences in DMD, NDFD, and ADFD across the storage conditions found significant interactions between the forage sample and the storage condition (p < 0.05). The results between the control and SC1, SC2, and SC3 were not different (p < 0.8). Fecal material stored aerobically for six hours at 22 °C provided similar digestibility estimates compared to the control, while DMD decreased by 3.86% in SC2 and by 4.08% in SC3.

scholarly journals Botanicals to Control Soft Rot Bacteria of Potato

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
M. M. Rahman ◽  
A. A. Khan ◽  
M. E. Ali ◽  
I. H. Mian ◽  
A. M. Akanda ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Curcuma Longa ◽  
Soft Rot ◽  
Storage Condition ◽  
Storage Conditions ◽  
Leaf Extracts ◽  
Corchorus Capsularis ◽  
Rot Disease ◽  
And Storage

Extracts from eleven different plant species such as jute (Corchorus capsularisL.), cheerota (Swertia chiraitaHam.), chatim (Alstonia scholarisL.), mander (Erythrina variegata), bael (Aegle marmelosL.), marigold (Tagetes erecta), onion (Allium cepa), garlic (Allium sativumL.), neem (Azadiracta indica), lime (Citrus aurantifolia), and turmeric (Curcuma longaL.) were tested for antibacterial activity against potato soft rot bacteria,E. carotovorasubsp.carotovora (Ecc)P-138, underin vitroand storage conditions. Previously,EccP-138 was identified as the most aggressive soft rot bacterium in Bangladeshi potatoes. Of the 11 different plant extracts, only extracts from dried jute leaves and cheerota significantly inhibited growth ofEccP-138in vitro. Finally, both plant extracts were tested to control the soft rot disease of potato tuber under storage conditions. In a 22-week storage condition, the treated potatoes were significantly more protected against the soft rot infection than those of untreated samples in terms of infection rate and weight loss. The jute leaf extracts showed more pronounced inhibitory effects onEcc-138 growth both inin vitroand storage experiments.

scholarly journals COLD STORAGE OF IN VITRO RUBUS GERMPLASM

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 695f-695
Author(s):  
Barbara M. Reed
Keyword(s):  
Tissue Culture ◽  
Cold Storage ◽  
Survival Rates ◽  
Storage Condition ◽  
Storage Conditions ◽  
Shoot Cultures ◽  
Ms Medium ◽  
Glass Tubes ◽  
Plastic Tissue Culture

In vitro cold storage of Rubus germplasm was investigated using several environmental conditons and types of storage containers. Shoot cultures of Rubus species and cultivars were grown in either tissue culture bags or 20 × 150 mm glass tubes and compared for plant condition and survival under various storage conditions. Cultures stored at 10 C in the dark were in poor condition after 6 months. Cultures kept at 4 C were in much better condition and had higher survival rates after 18 months when stored with a 12 h daylength rather than total darkness. Overall there were no differences in survival or condition between cultures in tubes and bags. Contamination rates were 15% in tubes and 0% in bags. Plants in tissue culture bags could be stored for 9 months at 25 C with 16 h light when the nitrogen level of the MS medium was reduced to 25% and the medium volume was increased from 10 to 20 ml per bag. Genotype differences were apparent under all conditions tested. The best storage condition for Rubus germplasm was 4 C with 12 h light. Plastic tissue culture bags were preferred over tubes due to lower contamination rates.

Duddingtonia flagranschlamydospores in nutritional pellets: effect of storage time and conditions on the trapping ability againstHaemonchus contortuslarvae

2013 ◽  
Vol 89 (1) ◽  
pp. 13-18 ◽  
Author(s):  
J.A. Fitz-Aranda ◽  
P. Mendoza-de-Gives ◽  
J.F.J. Torres-Acosta ◽  
E. Liébano-Hernández ◽  
M.E. López-Arellano ◽  
...  
Keyword(s):  
Storage Time ◽  
Agar Plate ◽  
Storage Condition ◽  
Storage Conditions ◽  
Crushed Material ◽  
Respective Control ◽  
The Mean ◽  
Control Batch

AbstractThe study evaluated the effect of storage time and conditions of nutritional pellets (NP) containingDuddingtonia flagranschlamydospores on itsin vitrotrapping ability againstHaemonchus contortusL3 larvae. The treated batch (200 NP) contained 4 ×  106chlamydospores of the FTH0-8 strain, whereas the control batch (200 NP) was produced without spores. Both NP batches were exposed to four experimental storage conditions: (T1) shelves (indoors); (T2) refrigeration (4°C); (T3) outdoors under a roof; and (T4) 100% outdoors. Each group comprised 48 NP with spores and 48 NP without spores (control). The ability ofD. flagransspores to trapH. contortusL3 larvae was evaluated for 8 weeks for each storage condition. For that purpose, six randomly selected NP with spores were compared to their respective control NP. Each NP was individually crushed. The crushed material (1 g) was placed on the surface of a 2% water agar plate with 200H. contortusL3 larvae. Plates were sealed and were incubated at room temperature for 8 days. The whole content of every plate was transferred to a Baermann apparatus to recover the remaining larvae. There was a clear larval reduction in the NP with spores, compared to the respective control NP in the four storage conditions (P< 0.05). The mean reductions ( ± SEM) of the storage conditions were 67 ± 4.9 (T2), 77 ± 6.1 (T1), 81.5 ± 3.8 (T4) and 82.1 ± 2.5 (T3). Larval reductions were similar at all times and were not affected by storage conditions or storage time (R2< 0.2;P>0.05). The long-term shelf-life of the chlamydospores in the NP suggests that this spore dosage technology is a viable option.

Revision: Indicadores del deterioro de la calidad proteica y del valor nutritivo de la leche / Review: Indicators of damage of protein quality and nutritional value of milk

1999 ◽  
Vol 5 (6) ◽  
pp. 447-461 ◽  
Author(s):  
E. Ferrer ◽  
A. Alegría ◽  
R. Farré ◽  
P. Abellán ◽  
F. Romero
Keyword(s):  
Maillard Reaction ◽  
Nutritional Value ◽  
Protein Quality ◽  
Storage Conditions ◽  
In Vitro Digestibility ◽  
Thermal Treatments ◽  
Maillard Reactions ◽  
Reducing Substances ◽  
Subsequent Storage

The nutritional value of milk can be negatively affected by thermal treatments. The quality of the proteins can be modified as a consequence of a set of reactions, the most important of which are the interactions between protein and carbohydrates, also known as Maillard reactions. These reactions can also occur when the storage conditions of moisture and temperature are unfavorable. The differ ent thermal treatments involved in processing milk (pasteurization, sterilization, drying, etc.) lead to different steps of the Maillard reaction and therefore to the formation of different compounds that can be useful as chemical markers of milk alteration, or more exactly of protein alteration. Estimating these markers is useful in monitoring the changes, especially those related to proteins, that happen during processing and storage. A review of the literature on the markers used to evaluate the modi fications that milk undergoes during thermal treatment and subsequent storage was carried out, focusing mainly on proteins. Special attention was paid to the analytical aspects. The chemical mark ers taken into account can be classified into two groups according to their relation with the Maillard reaction: 1. Specific markers of Maillard reaction: undesirable compounds (furosine, lisinoalanine, histidinalanine, furfurals, melanoidins); loss of nutrients (available lysine). 2. Non-markers of Maillard reaction: galactose; lactulose; protein-reducing substances; denaturation of protein; in vitro digestibility of proteins; others—pH, viscosity, free fatty acids.

Equine in Vitro Digestibility Using Different Cecal Fluid Dilutions

2021 ◽  
Vol 99 (Supplement_2) ◽  
pp. 46-47
Author(s):  
Cody Davis ◽  
Russell C Carrell ◽  
Serena Thompson ◽  
Madison Cole ◽  
S Leanne L Dillard
Keyword(s):  
Nutritive Value ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Fecal Material ◽  
Abdominal Incision ◽  
Fiber Analysis ◽  
Three Samples ◽  
Plastic Bag ◽  
Veterinary School

Abstract In vitro digestibility (IVTD) is a useful tool for determining nutritive value of forages. The majority of equine forage digestion occurs in the cecum; however, many studies use fecal inoculum. The current study aimed to determine the optimal buffer:cecal fluid for batch culture fermentation and compare this method with fecal IVTD. Three horses were sourced from the Auburn University Veterinary School. Fecal material was collected via a rectal grab sample, placed in a plastic bag, and into a 39°C water bath. The cecal contents were collected using an abdominal incision within 5 min of termination. Contents were placed in a pre-warmed (39°C) thermos. Cecal fluid was strained and proportioned into 3 treatments (100:0, 75:25, and 50:50 cecal fluid:buffer). Fecal material was weighed and mixed with buffer to achieve a 10:90 by weight. Three samples of alfalfa, bermudagrass, and perennial peanut hay were randomly assigned to a fluid treatment and placed in the respective jar, digested for 48h, then underwent neutral detergent fiber analysis. The experiment was designed as a 4 × 3 factorial with horse considered random (n = 9). Statistical analysis was conducted using Proc Mixed (SAS Inst, Cary, NC) with α = 0.05. There was no effect of fluid treatment on IVTD (P = 0.12). However, a forage effect was observed (P &lt; 0.001). Alfalfa and perennial peanut IVTD were not different (75.6%; P = 0.40), but greater (P &lt; 0.001) than bermudagrass (48.1%). This study showed that no difference in cecal fluid and fecal material occurred when used as microbial inoculant sources for a Daisy-II batch fermentation for determination of equine IVTD.

scholarly journals COMPARISON OF SIX COMMERCIAL HAY PRESERVATIVES UNDER SIMULATED STORAGE CONDITIONS

1988 ◽  
Vol 68 (4) ◽  
pp. 1195-1207 ◽  
Author(s):  
V. S. BARON ◽  
G. G. GREER
Keyword(s):  
Organic Acids ◽  
Propionic Acid ◽  
Dry Matter ◽  
Storage Conditions ◽  
Sodium Sulphate ◽  
Aerobic Bacteria ◽  
In Vitro Digestibility ◽  
Mould Growth ◽  
Dry Matter Loss

Rehydrated alfalfa (Medicago sativa L.) hay was used to test the effectiveness of six commercial hay additives in the laboratory. The additives were designated B1 (live bacterial culture, protease and cellulose enzymes) P1 (67% propionic acid partly neutralized with ammonia) R2 (25% propionic acid and unspecified amounts of other organic acids and flavorings), S (92% sodium sulphate), P3 (19% propionic acid and unspecified amounts of other organic acids and flavorings) and B2 (12% lactic acid and fermentation extract). The additives were assessed on their ability to prevent deterioration in in vitro digestible organic matter (IVDOM), heating and mould growth, loss of dry matter and associated parameters at hay moistures ranging from 15 to 35% moisture with additive rates specified by the manufacturer. After 21 d of storage and at all moisture levels P1 (1.25% wt wt−1) maintained IVDOM and most other parameters at levels similar (P ≤ 0.05) to an unspoiled Control at 15% moisture. P2 (0.2% wt wt−1) reduced heating and mould growth and maintained IVDOM to the levels of unspoiled hay up to 25% moisture. P3 (0.3% wt wt−1) provided slight improvements in IVDOM up to 25% moisture, but did not prevent heating. S (0.1% wt wt−1) limited dry matter loss at 20% moisture. Alfalfa hays treated with B1 (0.1% wt wt−1) or B2 (80 or 100 mL t−1 of dry matter) were not different from the control hay for any parameters. At 25% moisture B1 and B2 did not affect the growth of anaerobic and aerobic bacteria or moulds while P1 inhibited the growth of moulds and aerobic bacteria. Based primarily on maintaining consistently high IVDOM at ≥ 25% moisture the order of effectiveness was P1 > P2 = P3 > S = B1 = B2. The slight effects of P2 and P3 on IVDOM may not be evident in the field. Key words: Rehydrated alfalfa hay, in vitro digestibility, microbial changes

In vitro digestibility of tempeh flours and preparation of instant soupmixes of greengram- Rice tempeh flour

2016 ◽  
Author(s):  
P. S. Lakshmy ◽  
K. T. Suman
Keyword(s):  
Chemical Modification ◽  
Storage Conditions ◽  
Green Gram ◽  
In Vitro Digestibility ◽  
Soybean Product ◽  
Genetic Breeding ◽  
Fermented Soybean ◽  
Dietary Starch

Dietary starch and proteins varies greatly in digestibility and its effects on the utilization of other nutrients. The variation appears to be due to differences in starch, protein and antinutrients affect the digestibility of foods. Processing treatments, storage conditions, chemical modification, and genetic breeding influence the digestibility of foods. Tempeh is a solid fermented soybean product that is consumed widely in Indonesia. Fresh tempeh using soyabean, green gram and rice were prepared. Tempeh flours were prepared from fresh tempeh. Invitro starch (IVSD) and protein (IVPD) digestibility of tempeh flours were analysed. The IVSD of different tempeh flours in the present study ranged from 72.18 to 83.86 per cent and IVPD ranged 75.12 to 90.86 per cent. Instant soup mixes were formulated using the tempeh flours.

Evaluation of Microbial Load from Canned Soya Milk Drinks in Malaysia

2016 ◽  
Vol 2 (1) ◽  
pp. 22-25
Author(s):  
Nur Amalina binti Mustafa ◽  
Muhammad Ashraf bin Redzuan ◽  
Muhamad Hazim bin Zuraimi ◽  
Muhamad Shuhaimi bin Shuib ◽  
Shahnaz Majeed ◽  
...  
Keyword(s):  
Culture Media ◽  
Storage Condition ◽  
Storage Conditions ◽  
Nutrient Agar ◽  
Microbial Load ◽  
Blood Agar ◽  
Human Consumption ◽  
Processing Unit ◽  
Soya Milk ◽  
Two Samples

Objective: Owing to the habit of consuming ready food among the citizens of Malaysia a study was conducted to evaluate 20 samples of canned soya milk for the presence of possible microbial content. The samples were collected randomly from shopping malls, restaurants and kiosk in Ipoh Malaysia. Methods: All samples collected across Ipoh, were subjected to test for presence bacteria in nutrient agar, blood agar and macConkey media. The possible microbial load was swapped from surface and soya milk content with a sterile cotton and streaked on nutrient agar, blood agar and macConkey culture media. The streaked petri plates were incubated for 48 hours at 37oC. Results: The study revealed negative microbial growth in all except two samples from the surface and soya milk content collected from a restaurant in nutrient agar and blood agar medium. The presence of microbes was conformed as gram positive staphylococcus sp. through gram staining. The positive growth may be imputed to poor storage condition at the restaurant. Conclusion: It can be computed from the study that the majority of the samples were free from bacterial growth, suggesting strong in house quality control mechanism at the processing unit and exquisite storage conditions in malls and kiosk suggesting that soya milk available in malls and kiosk are fit for human consumption.

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