Evaluation of Microbial Load from Canned Soya Milk Drinks in Malaysia

2016 ◽  
Vol 2 (1) ◽  
pp. 22-25
Author(s):  
Nur Amalina binti Mustafa ◽  
Muhammad Ashraf bin Redzuan ◽  
Muhamad Hazim bin Zuraimi ◽  
Muhamad Shuhaimi bin Shuib ◽  
Shahnaz Majeed ◽  
...  
Keyword(s):  
Culture Media ◽  
Storage Condition ◽  
Storage Conditions ◽  
Nutrient Agar ◽  
Microbial Load ◽  
Blood Agar ◽  
Human Consumption ◽  
Processing Unit ◽  
Soya Milk ◽  
Two Samples

Objective: Owing to the habit of consuming ready food among the citizens of Malaysia a study was conducted to evaluate 20 samples of canned soya milk for the presence of possible microbial content. The samples were collected randomly from shopping malls, restaurants and kiosk in Ipoh Malaysia. Methods: All samples collected across Ipoh, were subjected to test for presence bacteria in nutrient agar, blood agar and macConkey media. The possible microbial load was swapped from surface and soya milk content with a sterile cotton and streaked on nutrient agar, blood agar and macConkey culture media. The streaked petri plates were incubated for 48 hours at 37oC. Results: The study revealed negative microbial growth in all except two samples from the surface and soya milk content collected from a restaurant in nutrient agar and blood agar medium. The presence of microbes was conformed as gram positive staphylococcus sp. through gram staining. The positive growth may be imputed to poor storage condition at the restaurant. Conclusion: It can be computed from the study that the majority of the samples were free from bacterial growth, suggesting strong in house quality control mechanism at the processing unit and exquisite storage conditions in malls and kiosk suggesting that soya milk available in malls and kiosk are fit for human consumption.

scholarly journals Effect of Pineapple Pulp on Sensory Quality and Shelf-life of Pineapple Cake

2015 ◽  
Vol 8 (1) ◽  
pp. 7-11
Author(s):  
MA Haque ◽  
S Afrin ◽  
AZ Shibly ◽  
FT Zohora ◽  
MM Sultana
Keyword(s):  
Shelf Life ◽  
Bacterial Load ◽  
Chemical Properties ◽  
Storage Condition ◽  
Storage Conditions ◽  
Food Samples ◽  
Viable Count ◽  
Microbial Load ◽  
Load Range ◽  
Pineapple Pulp

The experimental study on performance formulation and analysis of pineapple pulp fortified cake considering physical, chemical, sensorial and microbial quality. Cake was fortified with 5%, 10%, 15% and 20% pineapple pulp incorporated into wheat flour. The pineapple pulps used in the cake preparation were analyzed for proximate composition. The physical and chemical properties of cakes were analyzed. Quantitive microbial analysis of food samples were done on the basis of total viable count. Bacterial load range from 1.22×102(cfug-1) to 9.54×103(cfug-1) on different storage condition. Zero present pineapple pulp incorporated cake sample showed the highest microbial load 9.54×103(cfug-1).Total fungal count ranged from 0.97×102 to 5.04×103 (cfug-1)on different storage conditions. Fortified cake with 10% pineapple pulp contains lowest amount of fungi and bacteria after 8 days. So its shelf life will be enhanced about 6-7 days. Further 5%, 15%, and 20% pineapple pulp incorporated cake sample contain high microbial load, hence they will get shelf life of 5-6 days. Fortification of cake with 5%, 10%, 15%, 20%, pineapple pulp increasedthe value of ash, fiber and moisture in comparing with control cake. The statistical analysis of organoleptic test response of sensory attributes revealed that color, flavor, texture and overall acceptability were decreased with the increase of pineapple pulp in the cake formulation.Symmetry, crust and crumb quality, crust color and consistency,color,texture of the crumb and microbial load of the cakes containing 10% pulp were better than those of cakes containing above 10% pineapple pulp.J. Environ. Sci. & Natural Resources, 8(1): 7-11 2015

scholarly journals Stability and Influence of Storage Conditions on Nanofibrous Film Containing Tooth Whitening Agent

Pharmaceutics ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 449
Author(s):  
Siriporn Okonogi ◽  
Adchareeya Kaewpinta ◽  
Pisaisit Chaijareenont
Keyword(s):  
Water Absorption ◽  
Storage Condition ◽  
Storage Conditions ◽  
Scanning Calorimetry ◽  
Adhesive Properties ◽  
Tooth Whitening ◽  
Electrospinning Technique ◽  
Long Term Storage ◽  
Whitening Agent ◽  
Nanofibrous Structure

Carbamide peroxide (CP), a tooth whitening agent, is chemically unstable. The present study explores stability enhancement of CP by loading in a nanofibrous film (CP-F) composed of polyvinyl alcohol/polyvinylpyrrolidone/silica mixture, using an electrospinning technique. Kept at a temperature range of 60–80 °C for 6 h, CP in CP-F showed significantly higher stability than that in a polymer solution and in water, respectively. Degradation of CP in CP-F could be described by the first order kinetics with the predicted half-life by the Arrhenius equation of approximately 6.52 years. Physicochemical properties of CP-F after long-term storage for 12 months at different temperatures and relative humidity (RH) were investigated using scanning electron microscopy, X-ray diffractometry, differential scanning calorimetry, and Fourier transform infrared spectroscopy. It was found that high temperature and high humidity (45 °C/75% RH) could enhance water absorption and destruction of the nanofibrous structure of CP-F. Interestingly, kept at 25 °C/30% RH, the nanofibrous structure of CP-F was not damaged, and exhibited no water absorption. Moreover, the remaining CP, the mechanical properties, and the adhesive properties of CP-F were not significantly changed in this storage condition. It is concluded that the developed CP-F and a suitable storage condition can significantly improve CP stability.

scholarly journals Refrigeration of COVID-19 Vaccines: Ideal Storage Characteristics, Energy Efficiency and Environmental Impacts of Various Vaccine Options

Energies ◽  
2021 ◽  
Vol 14 (7) ◽  
pp. 1849
Author(s):  
Alexandre F. Santos ◽  
Pedro D. Gaspar ◽  
Heraldo J. L. de Souza
Keyword(s):  
Energy Efficiency ◽  
Environmental Impacts ◽  
Thermal Load ◽  
Storage Condition ◽  
Storage Conditions ◽  
Energy Simulation ◽  
Energy Usage ◽  
Impact Simulation ◽  
Storage Characteristics ◽  
The Ideal

This article considers the ideal storage conditions for multiple vaccine brands, such as Pfizer, Moderna, CoronaVac, Oxford–AstraZeneca, Janssen COVID-19 and Sputnik V. Refrigerant fluid options for each storage condition, thermal load to cool each type of vaccine and environmental impacts of refrigerants are compared. An energy simulation using the EUED (energy usage effectiveness design) index was developed. The Oxford–AstraZeneca, Janssen COVID-19 and CoronaVac vaccines show 9.34-times higher energy efficiency than Pfizer. In addition, a TEWI (total equivalent warming impact) simulation was developed that prioritizes direct environmental impacts and indirect in refrigeration. From this analysis, it is concluded that the cold storage of Oxford–AstraZeneca, Janssen COVID-19 and CoronaVac vaccines in Brazil generates 35-times less environmental impact than the Pfizer vaccine.

Effect of Different Storage Conditions on Soybean Oil Acid Value

2014 ◽  
Vol 962-965 ◽  
pp. 1235-1238
Author(s):  
Mei Mei Hao ◽  
Xi Hong Li ◽  
Hai Dong Liu ◽  
Wei Qiao Yang ◽  
Chong Xiao Shao ◽  
...  
Keyword(s):  
Moisture Content ◽  
Soybean Oil ◽  
Acid Value ◽  
Storage Condition ◽  
Storage Conditions

Under the condition of nine kinds of storage, In this thesis, through the determination of acid value of soybean oil, to search for the best storage condition, used to prevent the soybean oil acid value rise, through the analysis of the acid value data, We find the best storage condition, is 0°C, 6% moisture content, and PVC plastic wrap packaging.

scholarly journals Botanicals to Control Soft Rot Bacteria of Potato

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
M. M. Rahman ◽  
A. A. Khan ◽  
M. E. Ali ◽  
I. H. Mian ◽  
A. M. Akanda ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Curcuma Longa ◽  
Soft Rot ◽  
Storage Condition ◽  
Storage Conditions ◽  
Leaf Extracts ◽  
Corchorus Capsularis ◽  
Rot Disease ◽  
And Storage

Extracts from eleven different plant species such as jute (Corchorus capsularisL.), cheerota (Swertia chiraitaHam.), chatim (Alstonia scholarisL.), mander (Erythrina variegata), bael (Aegle marmelosL.), marigold (Tagetes erecta), onion (Allium cepa), garlic (Allium sativumL.), neem (Azadiracta indica), lime (Citrus aurantifolia), and turmeric (Curcuma longaL.) were tested for antibacterial activity against potato soft rot bacteria,E. carotovorasubsp.carotovora (Ecc)P-138, underin vitroand storage conditions. Previously,EccP-138 was identified as the most aggressive soft rot bacterium in Bangladeshi potatoes. Of the 11 different plant extracts, only extracts from dried jute leaves and cheerota significantly inhibited growth ofEccP-138in vitro. Finally, both plant extracts were tested to control the soft rot disease of potato tuber under storage conditions. In a 22-week storage condition, the treated potatoes were significantly more protected against the soft rot infection than those of untreated samples in terms of infection rate and weight loss. The jute leaf extracts showed more pronounced inhibitory effects onEcc-138 growth both inin vitroand storage experiments.

scholarly journals CHARACTERIZATION OF BACTERIA ASSOCIATED WITH PNEUMONIA IN BLACK BENGAL GOATS

2012 ◽  
Vol 9 (1) ◽  
pp. 67-71 ◽  
Author(s):  
MA Momin ◽  
MA Islam ◽  
MM Khatun ◽  
MM Rahman ◽  
MA Islam
Keyword(s):  
Antimicrobial Agents ◽  
Research Work ◽  
Nutrient Agar ◽  
Blood Agar ◽  
Biochemical Tests ◽  
Highly Sensitive ◽  
Golden Yellow ◽  
Agar Media ◽  
Staphylococcus Spp

The present research work was undertaken for the characterization of the bacterial pathogens responsible for pneumonia in black Bengal goats. Nasal swab samples (n = 50) were collected from the pneumonic black Bengal goats in Mymensingh and Sirajgonj districts. Samples were inoculated onto nutrient agar, eosin methylene blue (EMB) agar, MacConkey agar, and blood agar media for isolation of bacteria. Identification of bacteria was performed by the Gram's staining method, cultural properties and biochemical tests. Antibiotic sensitivity of bacterial isolates was performed against 11 antimicrobial agents. Pasteurella spp were isolated from 25 cases, and Staphylococcus spp from 13 cases. Mixed infection caused by the Pasteurella spp and Staphylococcus spp. were recorded in 12 cases. Pasteurella spp produced whitish, opaque circular and translucent colonies on nutrient agar, smooth, convex, glistening colonies on EMB agar and no hemolysis on blood agar. Staphylococcus spp have shown gray white or golden yellowish colonies on  nutrient agar. Golden yellow colonies without hemolysis or whitish colonies with hemolysis were also produced by Staphylococcus spp. on the blood agar media. Pasteurella spp were indole positive, MR-VP negative and ferment dextrose, sucrose and mannitol with the production of acid. The Staphylococcus spp were positive to MR-VP, coagulase and catalase reactions, negative to indole test and fermented five basic sugars with acid production. Results of cultural and biochemical tests supported that these two isolates belonged to P. multocida and S. aureus. P. multocida were highly sensitive to ciprofloxacin and resistant to penicillin. S. aureus found to be highly sensitive to erythromycin, tetracycline, enrofloxacin, and norfloxacin and less sensitive to amoxicillin. DOI = http://dx.doi.org/10.3329/bjvm.v9i1.11215Bangl. J. Vet. Med. (2011). 9(1): 67-71 

scholarly journals A review on potato microtuber storability and dormancy

2016 ◽  
Vol 8 (4) ◽  
pp. 2319-2324 ◽  
Author(s):  
Murlidhar J. Sadawarti ◽  
K. K. Pandey ◽  
B. P. Singh ◽  
R. K. Samadiya
Keyword(s):  
Significant Role ◽  
Carbon Disulphide ◽  
Potato Production ◽  
Storage Condition ◽  
Storage Conditions ◽  
Dormancy Breaking ◽  
Tuber Dormancy ◽  
Seed Potato Production ◽  
Sprouting Ability ◽  
And Storage

Potato microtubers plays important role in seed potato production technology as it has great advantage of storage, transport and mechanization due to their little size and reduced weight. Dormancy in potato microtubers is very important and storage conditions as well as size of microtubers influences the dormancy in microtubers. Increasing size of the micro-tuber resulted in significant increase in the viability and sprouting ability of microtubers with reduced durations of dormancy and weight loss at the end of storage. Small microtubers are more vulnerable to storage damage. The larger microtubers lost moisture content more slowly and retained firmness longer when stored at 40C. Development of dormancy during storage strongly affected by the storage condition especially the temperature regime, the presence of light and the relative humidity. The dormancy duration was linearly and inversely correlated with the length of storage. Storage containers and conditions are also important for microtuber storage. Endogenous hormones ABA, ethylene, cyokinin and gibberllic acid play a significant role in tuber dormancy regulation.Microtubers with thick diameter which have passed more times in dormancy and have better functionality than small microtubers with less time in dormancy. Growth regulators like gibberellic acid, thiourea, gibberllic acid + thiourea, randite and carbon disulphide plays significant role in dormancy breaking of microtubers.

Correlation between mastitis occurrence and the count of microorganisms in bulk raw milk of bovine dairy herds in four selective culture media

2009 ◽  
Vol 77 (1) ◽  
pp. 63-70 ◽  
Author(s):  
Luís I M Souto ◽  
Clarice Y Minagawa ◽  
Evelise O Telles ◽  
Márcio A Garbuglio ◽  
Marcos Amaku ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Negative Correlation ◽  
Culture Media ◽  
Agar Plate ◽  
Raw Milk ◽  
Blood Agar ◽  
Plate Count ◽  
Dairy Herds ◽  
Potassium Tellurite ◽  
Positive Correlations

Milk is the normal secretion of the mammary gland, practically free of colostrum and obtained by the complete milking of one or more healthy animals. Mastitis is an inflammatory process of the mammary gland and it may cause alterations in the milk. The present work aimed to verify whether it is possible, by means of the counts of microorganism in the bulk raw milk in four selective culture media, to establish a correlation with the occurrence of mastitis and therefore, to monitor this disease in bovine dairy herds. The following selective culture media were used: KF Streptococcus Agar, Edwards Agar, Baird-Parker Agar, Blood Agar plus potassium tellurite. Spearman's correlation coefficient was calculated in order to compare the occurrence of mastitis (percentage) in each herd with respective selective culture media counts of microorganisms in bulk raw milk. Thirty-six possibilities were analysed (Tamis and CMT-positive rates were compared with the log-transformed count in four selective culture media) and there was a negative correlation between Tamis 3 and the Baird-Parker Agar plate count. The total results of microbiological tests showed that there were three correlations of the counts in selective culture media. Fifty-two possibilities were analysed and there was a negative correlation between no-bacterial-growth mastitis rates and log10 of KF Streptoccocus Agar plate count and there were two positive correlations between coagulase-positive staphylococci and log10 of Baird-Parker Agar plate count and Blood Agar plus potassium tellurite plate count.

scholarly journals Microbiological quality of commercially available poultry feeds sold in Bangladesh

2017 ◽  
Vol 3 (1) ◽  
pp. 52-60
Author(s):  
Nigar Sultana ◽  
Md Atiqul Haque ◽  
Md Mostafizer Rahman ◽  
Mir Rowshan Akter ◽  
Mst Deloara Begum ◽  
...  
Keyword(s):  
Public Health ◽  
Methylene Blue ◽  
Culture Media ◽  
Microbiological Quality ◽  
Control Measures ◽  
Nutrient Agar ◽  
Poultry Feed ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
E Coli

The study was conducted aiming at the isolation and identification of pathogens from poultry feed manufactured by four different brands namely A (broiler starter), B (broiler finisher), C (layer starter) and D (layer finisher) sold in retail stores of Rangpur city of Bangladesh. All these samples were collected from four randomly chosen outlets and analyzed by culturing in different culture media such as Nutrient broth (NB), Nutrient agar (NA), Salmonella-Shigella (SS) agar, Eosin methylene blue (EMB) agar, MacConkey agar, Triple sugar iron (TSI) agar slant, Motility, Indole, Urease (MIU) and Saboraud Dextrose agar (SDA) media. The bacterial agents were isolated and examined under light microscope for their gross morphological and conventional biochemical characteristics. The bacteriological analyses were done at the Microbiology Laboratory of Hajee Mohammad Danesh Science and Technology University, Dinajpur during the period of January to June, 2014. Total bacterial colonies of all the samples were counted separately according to the American Public Health Association, using nutrient agar medium for total viable count (TVC), Eosine methylene blue (EMB) agar media for total E. coli count (TEC) and Salmonella-Shigella agar for TSC (total salmonella count). Saboraud Dextrose agar (SDA) media was used for detection of fungus. The virulence effect of the organism present in feed were observed by inoculating the organism in poultry. Recorded result showed that average TVC of feed sample A, B, C and D were 5.45x106, 3.28x105, 5.14x106 and 4.53x105 CFU/gm (colony forming unit per gram) respectively. TEC of feed sample A, B, C and D were recorded 6.25x105, 8.26x103, 5.52x105 and 5.65x104 CFU/gm respectively. TSC of feed sample A, B, C and D were recorded 3.15x104, 2.68x103, 4.46x103 and 1.19x104 CFU/gm respectively. The highest TVC, TEC and TSC were found in broiler starter (feed sample A) and lowest TVC, TEC and TSC were found in broiler finisher (feed sample B). Fungal count was 1.85x105 CFU/ gm in layer finisher (feed sample D) could be as a result of their high pathogenecity as reported by researchers elsewhere. These organisms can cause several poultry and farm animal infections specially mycotoxicosis having public health significance to both human and poultry. The presence of high numbers of E. coli and Salmonella spp. in poultry feed were indicative of poor hygienic practices during manufacture, post process contamination and unsatisfactory transportation and reservation. Therefore reinforce the need for preventive control measures, hygienic handling and processing of feeds to reduce the risk of potential human health hazards.Asian J. Med. Biol. Res. March 2017, 3(1): 52-60

scholarly journals PHYSIOLOGICAL AND SANITARY QUALITY AND OIL CONTENT OF CASTOR BEAN SEEDS UNDER DIFFERENT STORAGE CONDITIONS?

2019 ◽  
Vol 32 (1) ◽  
pp. 131-142
Author(s):  
Heloisa Oliveira dos Santos ◽  
Maria Laene Moreira de Carvalho ◽  
Camila Aparecida Lopes ◽  
Édila Vilela de Resende Von Pinho ◽  
Stefânia Vilas Boas Coelho
Keyword(s):  
Castor Bean ◽  
Oil Content ◽  
Seed Quality ◽  
Ricinus Communis ◽  
Storage Condition ◽  
Storage Conditions ◽  
Ideal Condition ◽  
Randomized Design ◽  
Cold Room ◽  
Bean Plants

ABSTRACT The objective of this work was to evaluate the effects of different storage conditions on the physiological and sanitary quality and oil content of castor bean (Ricinus communis) seeds. Seeds of castor bean plants of the Guarani, and IAC-80 cultivars were stored in two environments (cold room, and room conditions), using three package types (multifoliate Kraft paper bag, and polyethylene bag, and polyethylene bag with vacuum at 1 atm). In addition, another storage condition was evaluated: cryopreservation (-196 °C) in foil paper bags. Seed quality was evaluated before storage and at 4, 8, and 12 months after storage by testing their 7-day and 14-day germination, emergence, health, water content, and oil content. The experiment was conducted in a completely randomized design, with 7×4 factorial arrangement consisting of seven storage conditions and four evaluation times. Cryopreservation is the ideal condition for maintaining the seed physiological quality of the Ricinus communis cultivars used throughout storage. The oil content of the R. communis seeds decreases, and the incidence of Aspergillus spp. and Fusarium spp. fungi increases throughout storage, regardless of the storage conditions.

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