scholarly journals Induction of Spermiation in Sterlet Acipenser ruthenus by PLGA Microparticle Delivery with Sustained Alarelin Release

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3305
Author(s):  
Peter Podhorec ◽  
Jindřiška Knowles ◽  
Jakub Vysloužil ◽  
Sergii Boryshpolets ◽  
Kateřina Kubová ◽  
...  
Keyword(s):  
Body Weight ◽  
Glycolic Acid ◽  
Saline Solution ◽  
Slow Release ◽  
Post Treatment ◽  
Sperm Production ◽  
Plga Microparticle ◽  
Acipenser Ruthenus ◽  
Optimal Effect ◽  
Sterlet Acipenser Ruthenus

Carp pituitary treatment versus poly (lactiac-co-glycolic acid) microparticles with slow release of Alarelin at 35 µg kg−1 or 200 µg kg−1 body weight to induce spermiation was compared in sterlet Acipenser ruthenus. All hormone treatments initially increased testosterone and 11-ketotestosterone, with a subsequent decline in testosterone but consistent high levels of 11-ketotestosterone at 48 and 72 h post-treatment. Spermiation did not differ between hormone-treated groups, and was not detected in controls receiving saline solution. Administration of the carp pituitary led to maximum sperm production 24 h post-treatment, followed by a decrease at 48 h post-treatment, with no sperm obtained at 72 h. The effect of Alarelin at 35 µg kg−1 bw and carp pituitary did not differ at 24 and 48 h post-treatment, whereas 200 µg kg−1 bw Alarelin was associated with significantly lower spermatozoon concentration 24 h post-treatment compared to carp pituitary, with no difference in milt volume. Higher relative sperm production was observed 48 h after injection of Alarelin at 200 µg kg−1 bw compared to carp pituitary. Spermatozoon motility was significantly higher in fish receiving Alarelin at 35 µg kg−1 bw than 200 µg kg−1 bw. The treatment with optimal effect on inducing spermiation was poly (lactic-co-glycolic acid) microparticles with slow release of Alarelin at 35 µg kg−1 bw.

Effect of mouse epidermal growth factor on fertility in rams

1990 ◽  
Vol 41 (6) ◽  
pp. 1147 ◽  
Author(s):  
BW Brown ◽  
PE Mattner ◽  
BA Panaretto ◽  
GH Brown ◽  
BD Gream ◽  
...  
Keyword(s):  
Body Weight ◽  
Survival Rate ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Post Treatment ◽  
Sperm Production ◽  
Teratogenic Effects ◽  
Progressive Motility ◽  
Epidermal Growth ◽  
Daily Sperm Production

Seminal characteristics and flock fertility were studied in Merino rams treated with mouse epidermal growth factor (EGF). Intravenous infusions of 105 8g EGF/kg body weight over 24 h in five rams had no statistically significant effect on daily sperm production measured from 6-10 weeks after dosing or on the percentages of spermatozoa that were unstained (nigrosinleosin stain) or morphologically normal. At 7-8 weeks post treatment, the percentage of spermatozoa exhibiting progressive motility was significantly reduced (P<0.05). By 9-10 weeks after treatment, the seminal characteristics were normal in all rams. In 1987, eight rams and in 1988, six ofthe same rams, were joined with ewes for 6 weeks (at a rate of 2%) 12 weeks after s.c. injection of 150 8g/kg body weight. Compared with the performance of control rams, EGF had no significant effect on ram fertility, nor did it produce any teratogenic effects in their progeny or effect their survival rate.

scholarly journals Ploidy Levels and Fitness-Related Traits in Purebreds and Hybrids Originating from Sterlet (Acipenser ruthenus) and Unusual Ploidy Levels of Siberian Sturgeon (A. baerii)

Genes ◽  
2020 ◽  
Vol 11 (10) ◽  
pp. 1164
Author(s):  
Sahana Shivaramu ◽  
Ievgen Lebeda ◽  
Doi Thi Vuong ◽  
Marek Rodina ◽  
David Gela ◽  
...  
Keyword(s):  
Body Weight ◽  
Ecological Interactions ◽  
Time Intervals ◽  
Ploidy Levels ◽  
Cumulative Survival ◽  
Reciprocal Hybrids ◽  
Acipenser Ruthenus ◽  
Sampling Points ◽  
Males And Females ◽  
Sterlet Acipenser Ruthenus

The present study aimed to investigate and compare fitness-related traits and ploidy levels of purebreds and hybrids produced from sturgeon broodstock with both normal and abnormal ploidy levels. We used diploid Acipenser ruthenus and tetraploid A. baerii males and females to produce purebreds and reciprocal hybrids of normal ploidy levels. Likewise, we used diploid A. ruthenus and tetraploid A. baerii females mated to pentaploid and hexaploid A. baerii males to produce hybrids of abnormal ploidy levels. Fertilization of ova of A. ruthenus and A. baerii of normal ploidy with the sperm of pentaploid and hexaploid A. baerii produced fully viable progeny with ploidy levels that were intermediate between those of the parents as was also found in crosses of purebreds and reciprocal hybrids of normal ploidy levels. The A. ruthenus × pentaploid A. baerii and A. ruthenus × hexaploid A. baerii hybrids did not survive after 22 days post-hatch (dph). Mean body weight and cumulative survival were periodically checked at seven-time intervals. The recorded values of mean body weight were significantly higher in A. baerii × pentaploid A. baerii hybrids than other groups at three sampling points (160, 252 and 330 dph). In contrast, the highest cumulative survival was observed in A. baerii × A. ruthenus hybrids at all sampling points (14.47 ± 5.70 at 497 dph). Overall, most of the studied sturgeon hybrids displayed higher mean BW and cumulative survival compared to the purebreds. The utilization of sturgeon hybrids should be restricted to aquaculture purposes because they can pose a significant genetic threat to native populations through ecological interactions.

scholarly journals Ancient Sturgeons Possess Effective DNA Repair Mechanisms: Influence of Model Genotoxicants on Embryo Development of Sterlet, Acipenser ruthenus

2020 ◽  
Vol 22 (1) ◽  
pp. 6
Author(s):  
Ievgeniia Gazo ◽  
Roman Franěk ◽  
Radek Šindelka ◽  
Ievgen Lebeda ◽  
Sahana Shivaramu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Damage ◽  
Dna Repair ◽  
Embryo Development ◽  
Hatching Rate ◽  
Embryo Survival ◽  
Dna Repair Mechanisms ◽  
Acipenser Ruthenus ◽  
Repair Mechanisms ◽  
Sterlet Acipenser Ruthenus

DNA damage caused by exogenous or endogenous factors is a common challenge for developing fish embryos. DNA damage repair (DDR) pathways help organisms minimize adverse effects of DNA alterations. In terms of DNA repair mechanisms, sturgeons represent a particularly interesting model due to their exceptional genome plasticity. Sterlet (Acipenser ruthenus) is a relatively small species of sturgeon. The goal of this study was to assess the sensitivity of sterlet embryos to model genotoxicants (camptothecin, etoposide, and benzo[a]pyrene), and to assess DDR responses. We assessed the effects of genotoxicants on embryo survival, hatching rate, DNA fragmentation, gene expression, and phosphorylation of H2AX and ATM kinase. Exposure of sterlet embryos to 1 µM benzo[a]pyrene induced low levels of DNA damage accompanied by ATM phosphorylation and xpc gene expression. Conversely, 20 µM etoposide exposure induced DNA damage without activation of known DDR pathways. Effects of 10 nM camptothecin on embryo development were stage-specific, with early stages, before gastrulation, being most sensitive. Overall, this study provides foundational information for future investigation of sterlet DDR pathways.

Role of Ca2+ in the IVM of spermatozoa from the sterlet Acipenser ruthenus

2017 ◽  
Vol 29 (7) ◽  
pp. 1319 ◽  
Author(s):  
Olga Bondarenko ◽  
Borys Dzyuba ◽  
Marek Rodina ◽  
Jacky Cosson
Keyword(s):  
Sperm Motility ◽  
Seminal Fluid ◽  
Mature Male ◽  
Sperm Maturation ◽  
Wolffian Duct ◽  
Testicular Spermatozoa ◽  
Acipenser Ruthenus ◽  
Sterlet Acipenser Ruthenus ◽  
Motility Parameters

The role of Ca2+ in sturgeon sperm maturation and motility was investigated. Sperm from mature male sterlets (Acipenser ruthenus) were collected from the Wolffian duct and testis 24 h after hormone induction. Testicular spermatozoa (TS) were incubated in Wolffian duct seminal fluid (WDSF) for 5 min at 20°C and were designated ‘TS after IVM’ (TSM). Sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. To investigate the role of calcium transport in the IVM process, IVM was performed (5 min at 20°C) in the presence of 2 mM EGTA, 100 µM Verapamil or 100 µM Tetracaine. No motility was observed in the case of TS (10 mM Tris, 25 mM NaCl, 50 mM Sucr with or without the addition of 2 mM EGTA). Both incubation of TS in WDSF and supplementation of the activation medium with Ca2+ led to sperm motility. The minimal Ca2+ concentration required for motility activation of Wolffian duct spermatozoa, TS and TSM was determined (1–2 nM for Wolffian duct spermatozoa and TSM; approximately 0.6 mM for TS). Motility was obtained after the addition of verapamil to the incubation medium during IVM, whereas the addition of EGTA completely suppressed motility, implying Ca2+ involvement in sturgeon sperm maturation. Further studies into the roles of Ca2+ transport in sturgeon sperm maturation and motility are required.

Ultrastructural Localization of Intracellular Calcium During Spermatogenesis of Sterlet (Acipenser ruthenus)

2016 ◽  
Vol 22 (6) ◽  
pp. 1155-1161 ◽  
Author(s):  
Amin Golpour ◽  
Martin Pšenička ◽  
Hamid Niksirat
Keyword(s):  
Intracellular Calcium ◽  
Developmental Stages ◽  
Physiological Function ◽  
Male Gamete ◽  
Ultrastructural Localization ◽  
Acrosomal Vesicle ◽  
Acipenser Ruthenus ◽  
Calcium Deposits ◽  
Late Stages ◽  
Sterlet Acipenser Ruthenus

AbstractCalcium regulates many intracellular events such as growth and differentiation during different stages of gamete development. The aim of this study was to localize and quantify the intracellular distribution of calcium during different developmental stages of spermatogenesis in sterlet, Acipenser ruthenus, using a combined oxalate–pyroantimonate technique. The distribution of calcium was described in spermatogonium, spermatocyte, spermatid, and spermatozoon stages. In the spermatogonium and spermatocyte, calcium deposits were mainly localized in the nucleus and cytoplasm. The spermatid had calcium in the nucleus, developing acrosomal vesicle, and cytoplasm. Intracellular calcium transformed from scattered deposits in spermatogonia and spermatocyte stages into an unbound form in spermatid and the spermatozoon. The proportion of area covered by calcium increased significantly (p<0.05) from early to late stages of spermatogenesis. The largest proportion of area covered by calcium was observed in the nucleus of the spermatozoon. In conclusion, although most of the intracellular calcium is deposited in limited areas of the spermatogonium and spermatocyte, it is present an unbound form in the larger area of spermatids and spermatozoa which probably reflects changes in its physiological function and homeostasis during the process of male gamete production in spermatogenesis.

The role of the IGF-I system for vitellogenesis in maturing female sterlet, Acipenser ruthenus Linnaeus, 1758

2007 ◽  
Vol 150 (1) ◽  
pp. 140-150 ◽  
Author(s):  
S. Wuertz ◽  
A. Nitsche ◽  
M. Jastroch ◽  
J. Gessner ◽  
M. Klingenspor ◽  
...  
Keyword(s):  
Acipenser Ruthenus ◽  
Igf I ◽  
Sterlet Acipenser Ruthenus

scholarly journals Daunorubicin associated to lipid core nanoparticles reduces atherosclerotic lesions, inflammation and cardiotoxicity in atherosclerosis rabbit model

2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
C.I Albuquerque ◽  
E.R Tavares ◽  
M.C Guido ◽  
N.M Lopes ◽  
R.V Baroni ◽  
...  
Keyword(s):  
Body Weight ◽  
Protein Expression ◽  
Post Treatment ◽  
Heart Weight ◽  
Funding Source ◽  
Lipid Core ◽  
Atherosclerotic Lesions ◽  
Apoptotic Factors ◽  
Aortic Lesions ◽  
Core Nanoparticles

Abstract Introduction Antiproliferative agents used in cancer chemotherapy have low toxicity and increased pharmacological action when carried into lipid core nanoparticles (LDE). LDE has similar structure to LDL and binds to LDL receptors through apo E it acquires in contact with plasma. Previously, we showed that treatment of rabbits with atherosclerosis with drugs such as taxanes and etoposide associate to LDE had pronounced reduction of the atherosclerotic lesions. In this study, we hypothesized whether daunorubicin (DNR) that belongs to another class of anti-cancer drugs, with a distinct mechanism of action and that has strong cardiotoxicity could also have effects against atherosclerosis. Methods Sixteen New Zealand male rabbits were fed with a 1% cholesterol diet for 8 weeks to induce atherosclerosis. After 4 weeks, the animals were treated weekly with LDE-DNR (6mg/kg, iv, n=9) or with LDE only (n=7). In addition, 3 animals without any intervention were used as a control group. Chow consumption, lipid and hematological profiles, body weight, and echocardiography were evaluated at baseline, pre-treatment and post-treatment. Morphometry and protein expression were performed to analyze the aortas. Results There was no difference in food intake and body weight for control, LDE and LDE-DNR groups. Total cholesterol, HDL-C, non-HDL-C and triglycerides increased in LDE and LDE-DNR groups when comparing baseline and post-treatment. Red blood cells decreased in LDE group at post-treatment in comparison to baseline, while LDE-DNR presented no such difference. Regarding aortic lesions of LDE-DNR group was 50% lower than LDE group. The protein expression of the inflammatory markers CD68, TNF-α and IL-6 in LDE-DNR was lower than LDE. Pro-apoptotic factors caspase 3, caspase 9 and BAX were also lower in LDE-DNR compared to LDE. Protein expression of vascular endothelial growth factor (VEGF) and vascular cell adhesion molecule (VCAM) was lower in the LDE-DNR group when compared to the LDE group. To test the cardiotoxicity of LDE-DNR, echocardiography was performed and observed that under LDE-DNR both systolic and diastolic function were preserved with no difference in cardiac mass in LDE-DNR when compared to control and LDE groups. However, LDE showed a significant increase in relative heart weight compared to control while LDE-DNR had no such difference. Treatment with LDE-DNR did not induce observable hematological toxicity. Conclusion The treatment with LDE-DNR reduced aortic lesions, diminished inflammatory and pro-apoptotic factors, diminished VCAM expression in the aorta and promoted cardiac preservation in a rabbit atherosclerosis model. In conclusion, LDE-DNR can be eligible for future developments in the quest for novel effective and safe treatments for atherosclerosis. Funding Acknowledgement Type of funding source: Public grant(s) – National budget only. Main funding source(s): FAPESP (Fundaçao de Amparo a Pesquisa do Estado de Sao Paulo)

Effects of antifreeze proteins on cryopreserved sterlet, Acipenser ruthenus sperm quality

Cryobiology ◽  
2018 ◽  
Vol 85 ◽  
pp. 184
Author(s):  
Miaomiao Xin ◽  
Jan Sterba ◽  
Anna Shaliutina-Kolesova ◽  
Borys Dzyuba ◽  
Serhii Boryshpolets ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Antifreeze Proteins ◽  
Acipenser Ruthenus ◽  
Sterlet Acipenser Ruthenus

scholarly journals The Effect of Venom of Egyptian Spitting Cobra Naja nubiae on Vascular Permeability of Hepatic and Renal Tissues

2021 ◽  
Vol 9 (1) ◽  
pp. 22-25
Author(s):  
Asmaa Saad Mahmoud Shokhba ◽  
Mohamed A. Abdel-Rahman ◽  
Mohammed Alaa El-Deen A. Omran ◽  
Nahla Soliman El-Shenawy
Keyword(s):  
Body Weight ◽  
Vascular Permeability ◽  
Saline Solution ◽  
Public Awareness ◽  
Control Group ◽  
Albino Rats ◽  
Colorimetric Determination ◽  
Crude Venom ◽  
Treatment Groups ◽  
Renal Vascular

Background: Among venomous elapid snakes, cobras have the highest public awareness, as their venom represents a combination of proteins, peptides, and enzymes that have a range of biochemical and pharmacological roles and are also the main constitutes of biological activity and lethal toxicity. Objectives: The study aimed to evaluate the effect of the venom of Egyptian Spitting Cobra, Naja nubiae, on the vascular permeability based on the extravasation of the azo dye Evans blue (EB) into the tissues of the liver and kidneys of animals envenomed with low (¼ LD50; 0.32 mg/kg) and high (½ LD50; 0.65 mg/ kg) doses at three sampling times (30, 120, 360 min) post-injection of the venom. Methods: Fifty-four adult male Albino rats (8 weeks old and 180±2 0 g body weight) were divided into three main groups (n=6). In the control group, rats were subcutaneously (SC) injected with saline solution. Envenomed groups were SC injected, one group with 0.32 mg/kg and the other group with 0.65 mg/kg body weight of crude venom, respectively. Rats were I.V injected with EB dye 20 minutes before SC injection with saline solution as control animals and with Naja nubiae venom as treatment groups. Results: The results illustrated a high significant rate of EB extravasation to hepatic and renal tissues by the colorimetric determination of EB dye concentration. Conclusion: The venom of Naja nubiae can cause increased hepatic and renal vascular permeability which may explain the inflammatory effect induced by this venom.

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