scholarly journals The Use of κ-Carrageenan in Egg Yolk Free Extender Improves the Efficiency of Canine Semen Cryopreservation

Animals ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 88
Author(s):  
Eunji Kim ◽  
Areeg Almubarak ◽  
Nabeel Talha ◽  
Il-Jeoung Yu ◽  
Yubyeol Jeon
Keyword(s):  
Egg Yolk ◽  
Control Group ◽  
Spermine Oxidase ◽  
Plant Polysaccharide ◽  
Red Seaweeds ◽  
Spermine Synthase ◽  
Progressive Motility ◽  
Overall Efficiency ◽  
Lower Expression

κ-Carrageenan is a plant polysaccharide derived from red seaweeds reported to possess potential medicinal and antioxidants activities. The present study aimed to identify the cryoprotective effects of κ-carrageenan on the quality of frozen-thawed canine semen. Twenty-eight ejaculates were collected and diluted in a Tris egg-yolk-free extender supplemented with various concentrations of κ-carrageenan (0.0%, 0.1%, 0.2%, 0.3%, and 0.5%). The addition of κ-carrageenan to the extender at a 0.2% concentration induced a significant increase in the total motility (TM) and the rapid progressive motility (RPM) of canine sperm. Among the experimental groups, the highest percentage of sperms with intact acrosomes was found in the 0.5% κ-carrageenan group (p < 0.05). Apoptosis levels were significantly lower in the 0.1% and 0.2% κ-carrageenan treatment. Moreover, sperm in the κ-carrageenan supplemented group showed a significantly higher expression of antiapoptotic (Bcl-2) and lower expression of NADPH oxidase (NOX5), spermine synthase (SMS), and spermine oxidase (SMOX) genes than those in the control group. In conclusion, the addition of κ-carrageenan to the freezing extender improved the overall efficiency of frozen-thawed dog spermatozoa.

COMPARATIVE CHARACTERISTICS OF RAM SEMEN FROZEN IN DIFFERENT EXTENDERS

2021 ◽  
pp. 63-78
Author(s):  
M.M. AYBAZOV ◽  
◽  
A.N. SHEVCHENKO ◽  
M.I. SELIONOVA ◽  
T.V. MAMONTOVA
Keyword(s):  
Egg Yolk ◽  
Quality Parameters ◽  
Semen Parameters ◽  
Progressive Motility ◽  
Kinematic Performance ◽  
Synthetic Media ◽  
Ram Sperm

Numerous studies have proved the necessity of egg yolk in synthetic media to dilute semen before its cryopreservation. However, at the same time, it has been demonstrated that its use can adversely affect the quality of frozen-thawed sperm. The present study compares the main quality parameters of ram sperm frozen using TRIS-based diluent with egg yolk and two egg yolk-free diluents (OvixCell® and AndroMed®). A slower deterioration in the kinematic performance of sperm cryopreserved in TRIS diluent with native egg yolk confirmed higher cryoprotective performance compared to commercial extenders containing no egg yolk. Significantly higher total and progressive motility was observed in TRIS-based medium with egg yolk (P< 0.05). This advantage was maintained after four hours of incubation and became more significant at the end of cultivation (after six hours) (P< 0.01). Thus, ram sperm frozen in egg yolk medium retained better motility than in egg yolk-free extenders, which allows predicting its higher bioavailability. Assessment of some semen parameters using CASA showed that there were no significant differences in motility between the three extenders immediately after thawing the straws. When assessed two hours after thawing, a diluent containing egg yolk (TRIS-based) was found to have higher results for some of the examined traits than phospholipid diluents.

The use of butylated hydroxytoluene in cryopreservation of boar semen

2016 ◽  
Vol 12 (2) ◽  
pp. 21-28
Author(s):  
Monika Trzcińska ◽  
Magdalena Baryła
Keyword(s):  
Dna Fragmentation ◽  
Sperm Quality ◽  
Annexin V ◽  
Egg Yolk ◽  
Butylated Hydroxytoluene ◽  
Preimplantation Embryos ◽  
Progressive Motility ◽  
Boar Semen ◽  
Boar Spermatozoa

The objective of the study was to determine the effect of butylated hydroxytoluene (BHT) on the quality and fertilizing capacity of frozen-thawed (FT) boar semen. Semen from five boars (36 ejaculates) was resuspended in lactose-egg yolk-glycerol extender supplemented with 0 (control), 1.0 (R1), 1.5 (R2) or 2.0 mM BHT (R3). Sperm quality was assessed based on motility (CASA; TM: total motility; PM: progressive motility), phosphatidylserine (PS) translocation across the plasma membrane (Annexin-V-FLuos Staining Kit) and DNA fragmentation (TUNEL Assay). The FT semen was also used for intrauterine artificial insemination (AI) of synchronized gilts. The fertilizing capacity of the FT semen was assessed on the basis of the gilt insemination rate and the number of morphologically normal embryos. The quality of the preimplantation embryos was determined by observing a TUNEL-positive reaction. The highest percentage of progressive motile and viable spermatozoa was noted in extender R3 (74.8 ±4.4% and 63.7 ±5.8%), as compared with the control (38.3 ±2.8% and 36.1 ±2.6%). The addition of BHT to the extender did not increase early apoptotic changes in the frozen-thawed spermatozoa with respect to the control. Irrespective of the variant of the extender, cryopreservation and thawing did not induce fragmentation in the boar spermatozoa. The highest number of morphologically normal embryos from inseminated gilts was observed in the case of semen cryopreserved in extender supplemented with 1.5 mM BHT. No significant differences were observed in DNA fragmentation in the expanded blastocysts from gilts inseminated with FT semen cryopreserved in the extenders analysed.

scholarly journals A 150 kDa Protein Derived from Bull Seminal Plasma Extended the Survival Time of Kacang Goat Sperm Stored at 5°C

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Suherni Susilowati ◽  
Imam Mustofa ◽  
Wurlina Wurlina ◽  
Indah Norma Triana ◽  
Suzanita Utama ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Artificial Insemination ◽  
Skim Milk ◽  
Egg Yolk ◽  
Control Group ◽  
Genetic Quality ◽  
Treatment Groups ◽  
Igf I ◽  
Complex Protein

Artificial insemination has proven to be an effective method for increasing population size and genetic quality of Kacang goats. However, innovation is required to maintain the quality of Kacang goat semen in storage. This study aimed to examine the effects of supplementing the 150 kDa protein assumed as IGF-I complex derived from bull seminal plasma in skim milk-egg yolk extender on the quality of Kacang goat sperm stored at 5°C. Twelve ejaculates collected from three Kacang goats were divided into three groups. In the control group (T0), the ejaculates were extended with skim milk-egg yolk only. In the treatment groups (T1 and T2), the ejaculates were extended with skim milk-egg yolk supplemented with the IGF-I complex protein at 12 μg and 24 μg/100 mL, respectively. The extended semen was stored at 5°C, and the viability, motility, intactness of the plasma membrane, malondialdehyde concentration, and apoptotic sperm percentage were evaluated daily for five days. The results showed that the T1 was the most effective treatment for maintaining Kacang goat semen at a quality acceptable for artificial insemination over five days of storage at 5°C. However, the T0 and T2 groups retained acceptable qualities for only three days at 5°C. It could be concluded that supplementation of 12 μg of the 150 kDa protein derived from bull seminal plasma per 100 mL extender successfully extended the life span of Kacang goat sperm for five days.

34 The effect of dilution method of beagle dog semen on the survival rate of cryopreserved spermatozoa after thawing

2019 ◽  
Vol 31 (1) ◽  
pp. 143
Author(s):  
S. W. Kim ◽  
C.-L. Kim ◽  
I. S. Jeon ◽  
Y. G. Ko ◽  
I.-S. Hwang
Keyword(s):  
Drug Testing ◽  
Semen Analysis ◽  
Egg Yolk ◽  
Control Group ◽  
Dilution Method ◽  
Computer Assisted ◽  
Motile Sperm ◽  
Optimal Method ◽  
Beagle Dog ◽  
Progressive Motility

The successful cryopreservation of spermatozoa of the beagle dog for AI is essential for the establishment of the genetic banks of drug detection dogs. The beagle dog is widely used for drug testing and chosen for breeding by breeders. However, the use of cryopreserved beagle semen is limited by the lower number of offspring of dog species. In this study, 3 highly trained beagle dogs were chosen and their semen was cryopreserved for the next generation. The effects of dilution methods of beagle semen were tested using a direct dilution method at RT and a 2-step dilution method at 5°C. As a control group, the effects of a direct dilution method of semen on the percentage of motile sperm and progressive motility were analysed by computer-assisted semen analysis system (SAIS, Korea), and abnormality of spermatozoa was examined by Diff Quik staining. A total of 9 samples from 3 dogs were extended in 4% glycerol containing Tris-egg yolk diluents at approximately 22 to 25°C. The diluted semen was cooled to 5°C within 2h. The packed 0.5-mL straws were placed 5cm above the surface of LN for 10min and then plunged in. A 2-step dilution method was conducted using the same procedures of freezing, but the first dilution was done with glycerol-free diluent. After cooling to 5°C within 2h, the second diluent with 8% glycerol was added to the same volume of diluted semen at 5°C and stabilised for 1h. After thawing for 45s at 37°C, the semen from the 2-step dilution method showed the higher percentage of motile sperm (65.4±6% v. 45.3±8%; P&lt;0.05) and progressive motility (41.6±5.3% v. 32.3±3.7%; P&lt;0.05). However, the abnormalities between groups showed no differences. The results suggest that the optimal method for freezing beagle dog spermatozoa is a 2-step dilution process that consists of the first dilution at RT and the second dilution with glycerol at 5°C into diluted semen.

Effect of different extenders for preservation of ram semen at 4°C

2016 ◽  
Author(s):  
Haneef A. Rather ◽  
Rafiqul Islam ◽  
Asloob A. Malik ◽  
Farooz A. Lone ◽  
Mohamad Naiem Banday
Keyword(s):  
Citric Acid ◽  
Sperm Motility ◽  
Egg Yolk ◽  
Morphological Abnormalities ◽  
Progressive Motility ◽  
Hypoosmotic Swelling Test ◽  
Live Sperm

The aim of this study was to investigate the effects of different extenders viz. Tris citric acid fructose egg yolk (TCFEY), Tris citric acid glucose egg yolk (TCGEY), Egg yolk citrate fructose (EYCF) and Egg yolk citrate glucose (EYCG) on the quality of ram spermatozoa during preservation at 4°C. Semen samples showing more than 3+ mass motility and 70% progressive motility were pooled and subsequently divided into four aliquots. Each aliquot was extended separately in four different extenders viz. TCFEY, TCGEY, EYCF and EYCG and stored at 4°C up to 72h. The quality of spermatozoa on the basis of percentage of sperm motility, live sperm, morphological abnormalities, intact acrosome and hypoosmotic swelling test (HOST) reacted spermatozoa was evaluated immediately after extension in particular extenders (0 h), 24 h, 48 h and 72 h after preservation at 4°C. The percent sperm motility was significantly (P<0.01) higher for TCFEY and TCGEY than EYCF and EYCG at 72 h of preservation at 4°C. The percent HOST reacted spermatozoa and intact acrosomes were significantly (P<0.01) higher and morphological abnormalities were significantly (P<0.01) lower for Tris based fructose extender than other three extenders at 72 h at 4°C. In conclusion, Tris citric acid fructose egg yolk (TCFEY) was found the best in maintaining the quality of ejaculated ram spermatozoa during preservation for 72 h at 4°C. 

scholarly journals Physical Quality of the First Egg of Japanese Quail (Coturnix japonica L.) after Given Liquid Herbal Concoction

Mangifera Edu ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 121-130
Author(s):  
Hasan Basri ◽  
Meilinda Pahriana Sulastri
Keyword(s):  
Drinking Water ◽  
Quality Index ◽  
Egg Quality ◽  
Egg Yolk ◽  
Coturnix Japonica ◽  
Control Group ◽  
Shell Weight ◽  
Egg Weight ◽  
Physical Quality

This study aims to determine the first egg's physical quality in Coturnix japonica L. after being given a liquid. The study is an experimental study with a Completely Randomized Design. In this study, the test animals were 24 Coturnix japonica L. randomly selected from 42 quail with 12 days old and average body weight of ± 37.67 g. They have been selected are divided into four treatment groups, i.e., P0: a control group with untreated drinking water, P1: 3 ml/liter water, P2: 6 ml/liter water, P3: 9 ml/liter water. Each treatment group consisted of 6 replications. Variables analyzed included egg weight, eggshell weight, yolk weight, albumen weight, egg yolk index, and egg quality index. The results showed that the addition of herbal concoction with the treatment of 3 ml, 6 ml, and 9 ml per 1 liter of drinking water on the physical quality of the first egg of Coturnix japonica L. including egg weight, yolk weight, albumen weight, shell weight, egg quality index, and yolk index statistically showed results were not significantly different (P>0.05 ), but the addition of herbal concoction did not have a negative effect on the physical quality of the first eggs of Coturnix japonica L.

scholarly journals Ram semen preserved at 0°C with soybean lecithin Tris-based extender substituted for egg yolk

2021 ◽  
Vol 34 (2) ◽  
pp. 192-197
Author(s):  
Jian-qing Zhao ◽  
Guo-liang Xiao ◽  
Wen-liang Zhu ◽  
Di Fang ◽  
Na Li ◽  
...  
Keyword(s):  
Soybean Lecithin ◽  
Egg Yolk ◽  
Control Group ◽  
Progressive Motility ◽  
Significant Difference ◽  
Acrosome Integrity ◽  
Normal Offspring ◽  
And Control ◽  
Ram Sperm ◽  
Artificial Vagina

Objective: The present study evaluated the preservation of ram semen at 0°C using soybean lecithin with a Tris-fructose extender.Methods: Semen was collected by artificial vagina ejaculation from six rams with proven fertility. High quality ejaculates were diluted by soybean lecithin (0.25%, 0.5%, 0.75%, 1.0%, 1.25%) using Tris-fructose extender and control (Tris-fructose egg yolk extender), respectively. The ejaculates were diluted to a concentration of 5×10<sup>8</sup> sperm/mL, followed by cooling to 0°C in 90 min and maintaining the temperature for 12 days. The diluted semen samples were examined and recorded for sperm progressive motility, acrosome integrity at 0, 24, 72, 144, 216, 288 h, respectively. Two hundred and twenty-three ewes were inseminated for 216 h with optimal soybean lecithin concentrated semen or control via trans-cervical insemination.Results: The results showed that there were no differences in sperm progressive motility at 0, 24, 72, and 144 h (p>0.05). After 216 h, the sperm progressive motility in the control group and 0.5% concentration groups was significantly higher when compared to 0.25% concentration (p<0.05). The 0.5% concentration group demonstrated the highest survival rate and had no difference with the control group (p>0.05). At 216 h, the sperm progressive motility of all groups was still above 50%. The acrosome integrity of all groups was decreased with prolongation of storage time, but there was no difference at each time point (p>0.05). There was no significant difference in the lambing rate and pregnancy rate between the 0.5% concentration group and the control group (p>0.05).Conclusion: These results suggest that ram sperm is capable of fertilization after preservation at 0°C with 0.5% of soybean lecithin in Tris-based extender substituted for egg yolk and produce normal offspring after insemination.

scholarly journals Influence of Chromium and Cadmium Addition on Quality of Japanese Quail Eggs

2008 ◽  
Vol 77 (4) ◽  
pp. 503-508 ◽  
Author(s):  
M. Skalická ◽  
B. Koréneková ◽  
P. Naď ◽  
J. Šály
Keyword(s):  
Japanese Quail ◽  
Egg Yolk ◽  
Control Group ◽  
Egg Mass ◽  
Negative Effect ◽  
Group 3 ◽  
Group 2 ◽  
Positive Effect ◽  
Group 1

The experiment was conducted to evaluate the effect of chromium (Cr) and cadmium (Cd) on the quality of Japanese quail eggs. The birds (n = 60) were divided into 3 groups of 20 animals each. Group 1 was the control group. Group 2 was administered 0.12 mg Cr/day (Cr group) per quail in drinking water, whereas Group 3 was given a combination of 0.12 mg Cd and 0.12 mg Cr/ day (Cd-Cr group). The distribution of Cd in eggs, the egg mass, and the strength and thickness of shells was determined after after Day 35 and 58 of administration. Addition of Cr had a positive effect on the weight of eggs. A significant decrease of eggshell strength (p < 0.05) and thickness was found after Day 35 and 58 of the experiment in the Cd-Cr group (3) in comparison to the Cr-only group (2) and the control group (1). The content of Cd was significantly lower (p < 0.05) in eggshells and in the egg yolk from the Cr-only group (2) in comparison to the Cd-Cr group (3) and the control group (1). The data show that addition of Cr can improve quality indicators of quail eggs and eliminate the negative effect of Cd.

scholarly journals Effect of outdoor access and increased amounts of local feed materials in the diets of hens covered by the gene-pool protection programme for farm animals in Poland on quality of eggs during peak egg production / Wpływ dostępu do wybiegu i zwiększonego udziału krajowych komponentów paszowych w żywieniu rodów kur objętych programem ochrony na jakość jaj w szczycie nieśności

2013 ◽  
Vol 13 (2) ◽  
pp. 327-339 ◽  
Author(s):  
Józefa Krawczyk ◽  
Zofia Sokołowicz ◽  
Sylwester Świątkiewicz ◽  
Ewa Sosin-Bzducha
Keyword(s):  
Rhode Island ◽  
Egg Production ◽  
Laying Hens ◽  
Egg Yolk ◽  
Farm Animals ◽  
Control Group ◽  
Standard Diet ◽  
Native Breed ◽  
Outdoor Area

Abstract The objective of the study was to test the hypothesis that eggs from native breed laying hens fed a diet containing increased amounts of local feed materials are not inferior in quality to eggs from laying hens receiving a standard diet but raised without outdoor access. The study involved Greenleg Partridge (Z-11) and Rhode Island Red hens (R-11). Within each breed, the control group (C) consisted of 60 hens kept on litter without outdoor access, stocked at 5 birds/m2 and fed a diet containing 65.3% of local feed materials. The experimental group (E) contained 60 layers maintained on litter with access to an outdoor area (11 m2 per bird) and fed a diet containing 77.1% of local feed materials. Eggs from hens of both breeds, which received diets containing increased proportions of local feed materials had lower weight but higher yolk percentage. The quality of eggshells from hens fed the diet with increased amounts of local materials was similar to that of eggshells from confined hens. Egg yolk lipids from experimental groups were characterized by a more beneficial n-6/n-3 acid ratio and elevated vitamin A levels. These eggs had better sensory scores for colour, flavour and aroma, which suggests that it is appropriate to raise native breeds of chickens with outdoor access and local feed materials can be used in extensive husbandry systems.

scholarly journals The effect of L-carnitine in Tris egg yolk-based diluent on the quality of Pasundan bull semen preserved in chilled condition

2020 ◽  
Vol 45 (3) ◽  
pp. 197-205
Author(s):  
I. Darussalam ◽  
R. I. Arifiantini ◽  
I. Supriatna ◽  
R. S. D. Rasad
Keyword(s):  
Sperm Concentration ◽  
Egg Yolk ◽  
Carnitine Supplementation ◽  
Equal Part ◽  
Liquid Form ◽  
Sperm Analysis ◽  
Progressive Motility ◽  
Bull Semen ◽  
Sperm Abnormalities

The study was conducted to investigatethe effect of L-Carnitine supplementation in Tris-eggyolk (TEY) diluents to optimize the quality of Pasundan bull liquid semen. Semen samples were collected from three Pasundan bulls (6 – 7 years old)usinganartificial vagina. Semen samples were evaluated macroscopically and microscopically, semen having ≥70% progressive motility, ≥500×106ml-1 sperm concentration and ≤ 20% sperm abnormalities were divided into 5 equal part. Each part  wasdiluted with TEYand supplemented with0 mM(control), 1 mM, 2 mM, 3 mM and 4 mM L-Carnitine. Liquidsemen was storedat5°C.Sperm motility was evaluated every 12 h  objectively using Computer Asissted Sperm Analysis (CASA) until progressive motilityreached 40%. The best diluent was demonstratedby progressive motility (PM)by TEY supplemented with 1 mM L-Carnitine(43.08±0.49%) that remained up to 108 h compared tothecontrol group (43.63±0.70%) that remained up to 72 h (P<0.05). In conclusion, 1 mM L-Carnitine in TEY was the best concentration for preservation of Pasundan bull semen stored in the liquid form. 

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