scholarly journals The Use of Biochemical Measurements to Identify Pre-Slaughter Stress in Pasture Finished Beef Cattle

Animals ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 503 ◽  
Author(s):  
Kate M.W. Loudon ◽  
Garth Tarr ◽  
David W. Pethick ◽  
Ian J. Lean ◽  
Rod Polkinghorne ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Beef Cattle ◽  
Muscle Glycogen ◽  
Rest Period ◽  
Stress Indicators ◽  
Blood Samples ◽  
Plasma Biomarkers ◽  
Biochemical Measurements ◽  
The Relationship ◽  
Muscle Glycogen Concentration

This study considered the relationship between pre-slaughter stressors and plasma biomarkers in 488 pasture-raised cattle across two experiments. The design aimed to test groups consisting of steer only, heifer only, and mixed sex cattle under direct kill versus rested (14 days in abattoir holding paddocks) protocols. In Experiment One, cattle were sourced from four farms, and transported by trucks and ships on the same day. In Experiment Two, cattle were sourced from four farms where a comparison was made between marketing via two commercial saleyards or direct farm gate consignment to abattoir. Blood samples were collected at exsanguination for subsequent analyses and relation to meat quality attributes. Muscle damage, as indicated by creatine kinase, is the biomarker most correlated to ultimate pH and muscle glycogen concentrations. A two-week rest period is effective for lowering this enzyme and improving muscle glycogen concentration. Although the cattle was subjected to a range of stress inducing treatments, we found that plasma biomarkers alone appeared insufficient for use as diagnostic stress indicators.

Effects of season and stocking density during transport on live weight and biochemical measurements of stress, dehydration and injury of rabbits at time of slaughter

2004 ◽  
Vol 78 (2) ◽  
pp. 285-292 ◽  
Author(s):  
J. de la Fuente ◽  
M. I. Salazar ◽  
M. Ibáñez ◽  
E. González de Chavarri
Keyword(s):  
Muscle Glycogen ◽  
Plasma Concentrations ◽  
Stocking Density ◽  
Live Weight ◽  
Blood Parameters ◽  
Blood Samples ◽  
Weight Losses ◽  
Ldh Activity ◽  
Biochemical Measurements ◽  
Muscle Glycogen Concentration

AbstractThe effects on rabbit welfare of transport in two seasons (summer and winter) and at two stocking densities (high stocking density: 12 rabbits per cage; low stocking density: 8 rabbits per cage) were studied. The loss of live weight during transport was calculated. At slaughter, blood samples were obtained and some physiological parameters related to stress were analysed: cortisol, creatine kinase (CK), lactate dehydrogenase (LDH), lactate, glucose, packed cell volume (PCV), osmolarity, and albumin and globulin concentrations. In addition, liver and muscle samples were taken and immediately frozen in liquid nitrogen for subsequent glycogen measurements.Season significantly affected the live-weight losses and plasma concentrations of cortisol (P < 0.01), CK, LDH, lactate (P < 0.001) and glucose (P < 0.01), as well as osmolarity (P < 0.001). Liver and muscle glycogen concentration were also affected by the season (P < 0.001). The loss of live weight was higher in rabbits transported in winter than in summer. The plasma concentrations of cortisol, lactate and glucose, CK and LDH activity, and osmolarity, as well as liver and muscle glycogen concentrations were higher in rabbits transported in summer than in winter. Stocking density had no effect on the analysed parameters. The high levels of the analysed blood parameters, which are related to stress, lead to the conclusion that the welfare of the rabbits transported in summer was more affected than those transported in winter.

Serum mineral concentrations in relation to parturition in beef heifers and cows fed conserved forage

1997 ◽  
Vol 77 (1) ◽  
pp. 63-68 ◽  
Author(s):  
Julie A. Small
Keyword(s):  
Beef Cattle ◽  
Beef Heifers ◽  
Serum Progesterone ◽  
Blood Samples ◽  
Calving Ease ◽  
Serum Zn ◽  
Ad Libitum ◽  
Calving Season ◽  
Mineral Concentrations ◽  
The Relationship

A study was undertaken to examine the relationship between serum mineral concentrations and calving ease in beef cattle fed conserved forage. Jugular blood samples were taken from multiparous cows (n = 6) and 2-yr-old primparous heifers (n = 9) that calved without assistance and 2-yr-old primparous heifers that required assistance with calving (n = 6) on days −7, 0 and +7 after parturition. Sera were analyzed for Ca, P, Mg, K, S, Na, Cu, Zn and B by ICAP and for progesterone by RIA. From 2 mo before and throughout the calving season cattle were given ad libitum round bale grass-legume silage (2.89% K; 3.4% P; 13% CP, 40% ADF; 38% DM). Heifers were also given 0.5 kg hd−1 d−1 of a grain supplement beginning 3 wk before calving. At calving, cows and heifers were in moderate body condition (5.26 ± 0.14 BCS) and heifers had reached 85% of mature body weight (475 ± 26 kg). Serum progesterone concentrations were highest (P < 0.05) at −7 d and dropped below 1 ng mL−1 at parturition and +7 d. Concentrations of serum Mg, S and K were highest (P < 0.05) whilst concentrations of P, B and Cu were lowest (P < 0.05) at parturition. However, serum Mg and S also differed (P < 0.05) among calving groups because concentrations were lowest for heifers with assisted calvings and, serum K concentrations were influenced (P < 0.05) by interaction between calving group and time because K concentrations increased at parturition only in cows and heifers with unassisted calvings. Serum Zn, Ca, and Na were not (P < 0.05) influenced by calving group or time. It is concluded that Mg, S, K, P, B and Cu metabolism are involved in the physiology of parturition and Mg, S and K metabolism may be particularly important for calving ease in primparous beef heifers fed conserved forage. Key words: Parturition, minerals, beef cattle, potassium, boron, magnesium

The Relationship of Serum Protein Bound Iodine Levels to Rates of Gain in Beef Cattle

1953 ◽  
Vol 12 (1) ◽  
pp. 3-9 ◽  
Author(s):  
H. O. Kunkel ◽  
R. W. Colby ◽  
Carl M. Lyman
Keyword(s):  
Beef Cattle ◽  
Serum Protein ◽  
Relationship Of ◽  
The Relationship

scholarly journals Factors Influencing Creatine Kinase Response in Youth National Team Soccer Players

2021 ◽  
pp. 194173812199938
Author(s):  
Gabor Schuth ◽  
Gyorgy Szigeti ◽  
Gergely Dobreff ◽  
Peter Revisnyei ◽  
Alija Pasic ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Prediction Models ◽  
Soccer Players ◽  
Linear Regression Models ◽  
Level Of Evidence ◽  
Internal Load ◽  
Youth Soccer ◽  
National Team ◽  
Weekly Training ◽  
The Relationship

Background: Previous studies have examined the relationship between external training load and creatine kinase (CK) response after soccer matches in adults. This study aimed to build training- and match-specific CK prediction models for elite youth national team soccer players. Hypothesis: Training and match load will have different effects on the CK response of elite youth soccer players, and there will be position-specific differences in the most influential external and internal load parameters on the CK response. Study Design: Prospective cohort study. Level of Evidence: Level 4. Methods: Forty-one U16-U17 youth national team soccer players were measured over an 18-month period. Training and match load were monitored with global positioning system devices. Individual CK values were measured from whole blood every morning in training camps. The dataset consisted of 1563 data points. Clustered prediction models were used to examine the relationship between external/internal load and consecutive CK changes. Clusters were built based on the playing position and activity type. The performance of the linear regression models was described by the R2 and the root-mean-square error (RMSE, U/L for CK values). Results: The prediction models fitted similarly during games and training sessions ( R2 = 0.38-0.88 vs 0.6-0.77), but there were large differences based on playing positions. In contrast, the accuracy of the models was better during training sessions (RMSE = 81-135 vs 79-209 U/L). Position-specific differences were also found in the external and internal load parameters, which best explained the CK changes. Conclusion: The relationship between external/internal load parameters and CK changes are position specific and might depend on the type of session (training or match). Morning CK values also contributed to the next day’s CK values. Clinical Relevance: The relationship between position-specific external/internal load and CK changes can be used to individualize postmatch recovery strategies and weekly training periodization with a view to optimize match performance.

scholarly journals Correlation between L-Lactate Concentrations in Beef Cattle, Obtained Using a Hand-Held Lactate Analyzer and a Lactate Assay Colorimetric Kit

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 926
Author(s):  
Daniela M. Meléndez ◽  
Sonia Marti ◽  
Luigi Faucitano ◽  
Derek B. Haley ◽  
Timothy D. Schwinghamer ◽  
...  
Keyword(s):  
Statistical Significance ◽  
Road Transportation ◽  
Long Distance ◽  
Suitable Alternative ◽  
Blood Samples ◽  
Time Points ◽  
Pooled Data ◽  
Red Angus ◽  
Relationship Of ◽  
The Relationship

Lactate is a product of anaerobic glycolysis, used in animal research as an indicator of muscle fatigue. Therefore, it has been used as an indicator of cattle response to long distance transportation. The aim of this study was to assess the relationship of L-lactate concentrations measured using a Lactate Scout+ analyzer and a traditional lactate assay colorimetric kit. Blood samples were collected by venipuncture from 96 steers (Black or Red Angus × Hereford/Simmental and Black or Red Angus × Charolais; 247 ± 38.2 kg BW) prior to loading (LO1) and after 36 h of transport, and prior to reloading and after an additional 4 h of road transportation, and on d 1, 2, 3, 5, 14, and 28 after transport. The Lactate Scout+ analyzer strip was dipped in blood at the time of sampling, while blood samples were collected into sodium fluoride tubes for use in the colorimetric analysis. Pearson correlations were calculated to assess the strength of the relationship between the experimental methods for the quantification of L-lactate concentrations. The magnitude and direction of the correlation, and the level of statistical significance varied over the observed time points, ranging from r = −0.03 (p = 0.75; LO1) to r = 0.75 (p < 0.0001; d 3). The correlation for the pooled data was weak but statistically significant (r = 0.33, p < 0.0001). Based on the low magnitude of the correlation due to variability across sampling time points in this study, the Lactate Scout+ analyzer is not a suitable alternative to a lab-based assay (considered the gold standard) for measuring L-lactate in transported cattle.

scholarly journals PSVII-3 Correlations between L-lactate concentrations obtained using a handheld lactate analyser and a lactate assay colorimetric kit in beef cattle transported by road

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 296-297
Author(s):  
Daniela M Meléndez ◽  
Sonia Marti ◽  
Luigi Faucitano ◽  
Derek B Haley ◽  
Timothy D Schwinghamer ◽  
...  
Keyword(s):  
Statistical Significance ◽  
Cost Effective ◽  
Road Transportation ◽  
Long Distance ◽  
Suitable Alternative ◽  
Blood Samples ◽  
Handheld Device ◽  
Time Points ◽  
Relationship Of ◽  
The Relationship

Abstract Blood metabolites are used to assess a variety of animal conditions for veterinary diagnosis and research. Concentration of metabolites in blood can be measured using a commercially-available lab-based assay or in real-time using a handheld device developed to be more time- and cost-effective than the lab-based method. Lactate is a product of anaerobic glycolysis, used in animal research as an indicator of muscle fatigue. Therefore, it has been used as an indicator of cattle response to long distance transportation. The aim of this study was to assess the relationship of L-lactate concentrations measured using a Lactate Scout+ analyzer (Lactate Scout, EFK Diagnostics, Barleben, Germany) and a lactate assay colorimetric kit (Lactate Assay Kit, Cell Biolabs Inc., San Diego, CA). Blood samples were collected by venipuncture from 96 steers (245 ± 35.7 kg BW) prior to (L1) and after 36 h, and prior to and after an additional 4 h of road transportation, and on d 1, 2, 3, 5, 14, and 28 after transport. The Lactate Scout+ analyzer strip was dipped in blood at the time of sampling, while blood samples were collected into sodium fluoride tubes for use in colorimetric analysis. Pearson correlations were calculated to determine the relationship between the experimental methods for the quantification of L-lactate concentrations. The strengths and levels of statistical significance of the correlation varied over the observed time points, r = -0.03, P = 0.75 (L1) to r = 0.75, P = &lt; 0.0001 (d 3). The correlation for the pooled data was weak but statistically significant (r = 0.33, P &lt; 0.001). Based on the experimental results, the Lactate Scout+ analyzer is not a suitable alternative to a lab-based assay for measuring L-lactate in transported cattle, due to variability across sampling time points and weak correlation with the traditional enzymatic method.

The Relationship of Certain Blood Constituents to Rate and Economy of Gain in Beef Cattle

1959 ◽  
Vol 18 (1) ◽  
pp. 314-322 ◽  
Author(s):  
R. L. Arthaud ◽  
A. B. Schultze ◽  
R. M. Koch ◽  
V. H. Arthaud
Keyword(s):  
Beef Cattle ◽  
Blood Constituents ◽  
Relationship Of ◽  
The Relationship

1 Effect of Conditioning, Rest, and Post-rest Transport Duration on Welfare Indicators of Beef Cattle Transported by Road

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 2-3
Author(s):  
Daniela M Melendez ◽  
Sonia Marti ◽  
Timothy D Schwinghamer ◽  
Derek B Haley ◽  
Karen S Schwartzkopf-Genswein
Keyword(s):  
Beef Cattle ◽  
Factorial Design ◽  
Random Effects ◽  
Fixed Effects ◽  
Rest Period ◽  
Flight Speed ◽  
Beef Calves ◽  
Welfare Indicators ◽  
Preliminary Results ◽  
And Performance

Abstract The aim of this study was to assess the effects of conditioning, rest, and post-rest transport duration on welfare indicators of 6–7 mo old beef calves. Three hundred and twenty-eight weaned calves (237 ± 29.7 kg BW) were randomly assigned to a 2 × 2 × 2 nested factorial design: conditioning, conditioned (C) or non-conditioned (N); rest, 0 (R0) or 8 (R8) h, and post-rest transport, 4 (T4) or 15 (T15) h. Calves were sampled prior to the first loading (L1), after 20h of transport, prior to and after the additional 4 or 15-h transport, and at 1, 2, 3, 5, 14, and 28 d after transport ended. Data were analyzed using the GLIMMIX procedure of SAS. Fixed effects included conditioning, transport and time nested within rest period, while random effects included animal and pen. Greater shrink (P &lt; 0.01) was observed in C than N calves after the initial 20-h transport. The N calves had greater (P &lt; 0.01) ADG than C calves between L1 and d 5, while C had greater (P &lt; 0.01) ADG than N calves between 14 and 28 d. L-lactate concentrations and flight speed were greater (P ≤ 0.05) in C than N calves between L1 and d 5. The R8-T4 calves had greater (P &lt; 0.01) ADG than R8-T15 calves between L1 and d 5. The R0-T4 calves had greater (P = 0.02) L-lactate concentrations than R0-T15 and R8-T4 calves on d 1. The R0 calves had greater (P &lt; 0.01) ADG than R8 calves between 14 and 28 d. Preliminary results show physiological, behavioral, and performance differences across treatments, however, additional indicators are required to accurately assess the effect of conditioning, rest, and post-rest transport durations on calf welfare.

scholarly journals Creatine Kinase and Lactate Dehydrogenase Responses After Different Resistance and Aerobic Exercise Protocols

2017 ◽  
Vol 58 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Gustavo A. Callegari ◽  
Jefferson S. Novaes ◽  
Gabriel R. Neto ◽  
Ingrid Dias ◽  
Nuno D. Garrido ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Resistance Exercise ◽  
Aerobic Exercise ◽  
Aerobic Training ◽  
Body Height ◽  
Blood Samples ◽  
Set Protocol ◽  
Experimental Protocols ◽  
Elevate Plasma

AbstractThe aim of this study was to investigate the responses of creatine kinase (CK) and lactate dehydrogenase (LDH) after performing different resistance and aerobic exercise protocols. Twelve recreationally trained men (age, 23.2 ± 5.6 years; body mass, 84.3 ± 9.3 kg; body height, 178.9 ± 4.5 cm; and BMI, 26.3 ± 2.3 kg·m2) volunteered to participate in this study. All subjects were randomly assigned to four experimental protocols (crossover): (a) aerobic training at 60% of VO2max, (b) aerobic training at 80% of VO2max, (c) a resistance exercise (RE) session with a bi-set protocol, and (d) an RE session with a multiple sets protocol. Blood samples were collected before, immediately after and 24 hours following the experimental protocols. After 24 hours, there was a significant increase in CK for the 80% of VO2max protocol vs. the bi-set RE session (p = 0.016). Immediately after the protocols, we observed a significant increase in LDH among certain groups compared to others, as follows: multiple sets RE session vs. 60% of VO2max, bi-set RE session vs. 60% of VO2max, multiple sets RE session vs. 80% of VO2max, and bi-set RE session vs. 80% of VO2max (p = 0.008, p = 0.013; p = 0.002, p = 0.004, respectively). In conclusion, aerobic exercise performed at 80% of VO2max appears to elevate plasma CK levels more than bi-set RE sessions. However, the bi-set and multiple sets RE sessions appeared to trigger greater levels of blood LDH compared to aerobic protocols performed at 60% and 80% of VO2max.

Role of glycogen concentration and epinephrine on glucose uptake in rat epitrochlearis muscle

1997 ◽  
Vol 272 (4) ◽  
pp. E649-E655 ◽  
Author(s):  
J. Jensen ◽  
R. Aslesen ◽  
J. L. Ivy ◽  
O. Brors
Keyword(s):  
Glucose Uptake ◽  
Muscle Glycogen ◽  
Similar Treatment ◽  
Glycogen Concentration ◽  
Spearman's Rho ◽  
Spearman’S Rho ◽  
Tracer Amount ◽  
Muscle Glycogen Concentration ◽  
Basal Glucose Uptake

The effects of diet-manipulated variations in muscle glycogen concentration and epinephrine on glucose uptake were studied in epitrochlearis muscles from Wistar rats. Both basal and insulin-stimulated glucose uptake [measured with a tracer amount of 2-[1,2-3H(N)]deoxy-D-glucose] inversely correlated with initial glycogen concentration (glycogen concentration vs. basal glucose uptake: Spearman's rho = -0.76, n = 84, P < 0.000001; glycogen concentration vs. insulin-stimulated glucose uptake: Spearman's rho = -0.67, n = 44, P < 0.00001). Two fasting-refeeding procedures were used that resulted in differences in muscle glycogen concentrations, although with similar treatment for the last 48 h before the experiment. In the rats with the lower glycogen concentration, basal as well as insulin-stimulated glucose uptake was elevated. The muscle glycogen concentration had no effect on epinephrine-stimulated glycogenolysis. Epinephrine, however, was found to reduce basal glucose uptake in all groups. These results suggest that 1) the glycogen concentration participates in the regulation of both basal and insulin-stimulated glucose uptake in skeletal muscle, 2) the magnitude of epinephrine-stimulated glycogen breakdown is independent of the glycogen concentration, and 3) epinephrine inhibits basal glucose uptake at all glycogen concentrations.

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