scholarly journals Species Distribution, Antimicrobial Resistance, and Enterotoxigenicity of Non-aureus Staphylococci in Retail Chicken Meat

Antibiotics ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 809
Author(s):  
Soo In Lee ◽  
Sun Do Kim ◽  
Ji Heon Park ◽  
Soo-Jin Yang
Keyword(s):  
Antimicrobial Resistance ◽  
Opportunistic Infections ◽  
Food Poisoning ◽  
Tetracycline Resistance ◽  
Chicken Meat ◽  
Fluoroquinolone Resistance ◽  
Coagulase Negative Staphylococci ◽  
Meat Samples ◽  
Resistance Rates ◽  
Retail Chicken Meat

Non-aureus staphylococci (NAS), including coagulase-negative staphylococci, have emerged as important causes of opportunistic infections in humans and animals and a potential cause of staphylococcal food poisoning. In this study, we investigated (i) the staphylococcal species profiles of NAS in in retail chicken meat, (ii) the phenotypic and genotypic factors associated with antimicrobial resistance in the NAS isolates, and (iii) the prevalence of classical and newer staphylococcal enterotoxin (SE) genes. A total of 58 NAS of nine different species were isolated from retail raw chicken meat samples. The occurrence of multidrug resistance in the NAS, particularly S. agnetis and S. chromogenes, with high resistance rates against tetracycline or fluoroquinolones were confirmed. The tetracycline resistance was associated with the presence of tet(L) in S. chromogenes and S. hyicus or tet(K) in S. saprophyticus. The occurrence of fluoroquinolone resistance in S. agnetis and S. chromogenes was usually associated with mutations in the quinolone resistance determining regions (QRDR) of gyrA and parC. In addition, the frequent presence of SE genes, especially seh, sej, and sep, was detected in S. agnetis and S. chromogenes. Our findings suggest that NAS in raw chicken meat can have potential roles as reservoirs for antimicrobial resistance and enterotoxin genes.

scholarly journals Prevalence and antimicrobial resistance of Salmonella serotypes isolated from retail chicken meat and giblets in Iran

2015 ◽  
Vol 9 (05) ◽  
pp. 463-469 ◽  
Author(s):  
Hamid Reza Sodagari ◽  
Zohreh Mashak ◽  
Amir Ghadimianazar
Keyword(s):  
Antimicrobial Resistance ◽  
Foodborne Pathogens ◽  
Chicken Meat ◽  
Resistance Testing ◽  
Retail Markets ◽  
Predominant Type ◽  
Amoxicillin Clavulanic Acid ◽  
Resistant Strains ◽  
Resistance Rates ◽  
Retail Chicken Meat

Introduction: Salmonella is one of the major foodborne pathogens responsible for outbreaks of foodborne illness in humans worldwide. Methodology: A total of 560 samples of chicken meat and giblets were collected from retail markets for Salmonella identification, serotyping, and antimicrobial resistance testing. Results: Salmonella was detected in 19.8% of samples. Among the five serotypes identified, S. Thompson was the predominant type (48.7%). High antimicrobial resistance rates were observed to nalidixic acid (92.8%), tetracycline (81%), trimethoprim (68.4%), sulfamethoxazole / trimethoprim (61.2%), streptomycin (56.7%), and kanamycin (36.9%). Although resistance to chloramphenicol (3.6%), amoxicillin-clavulanic acid (5.4%), and ampicillin (11.7%) was detected, none of the isolates were resistant to ceftazidime, ceftriaxone, cefotaxime, ciprofloxacin, colistin, gentamicin, nor imipenem. Conclusions: Restrictions on the irrational use of antibiotics in humans and animals are suggested for the reduction of resistant strains.

scholarly journals Profiles of coagulase-positive and -negative staphylococci in retail pork: prevalence, antimicrobial resistance, enterotoxigenicity, and virulence factors

2021 ◽  
Vol 34 (4) ◽  
pp. 734-742
Author(s):  
Gi Yong Lee ◽  
Soo-Jin Yang
Keyword(s):  
Antimicrobial Resistance ◽  
Zinc Chloride ◽  
Food Poisoning ◽  
Pork Meat ◽  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant ◽  
Chloride Resistance ◽  
Antimicrobial Resistance Genes ◽  
Meat Samples ◽  
High Level

Objective: The present study aimed to investigate the occurrence and species of coagulasepositive staphylococci (CoPS) and coagulase-negative staphylococci (CoNS) in retail pork meat samples collected during nationwide monitoring. The staphylococcal isolates were characterized for antimicrobial and zinc chloride resistance and enterotoxigenic potential.Methods: A total of 260 pre-packaged pork meat samples were collected from 35 retail markets in 8 provinces in Korea for isolation of staphylococci. Antimicrobial and zinc chloride resistance phenotypes, and genes associated with the resistance phenotypes were determined on the isolates. Furthermore, the presence and distribution of 19 staphylococcal enterotoxin (SE) genes and enterotoxin-like genes among the pork-associated staphylococci were determined by multiplex polymerase chain reaction-based assays using the specific primer sets.Results: A total of 29 staphylococcal strains (29/260, 11.1%) were isolated from samples of retail pork meat, 24 (83%) of which were CoNS. The four CoNS species identified were <i>S. saprophyticus</i> (n = 16, 55%), <i>S. sciuri</i> (n = 3, 10%), <i>S. warneri</i> (n = 3, 10%), and <i>S. epidermidis</i> (n = 2, 7%). Among the 29 isolates, four methicillin-resistant CoNS (MR-CoNS; three <i>S. sciuri</i> and one <i>S. epidermidis</i>) and one methicillin-resistant CoPS (MR-CoPS; one <i>S. aureus</i>) were identified. In addition, a relatively high level of tetracycline (TET) resistance (52%) was confirmed in CoNS, along with a predominant distribution of <i>tet</i>(K). The most prevalent SEs were <i>sep</i> (45%), and <i>sen</i> (28%), which were carried by 81% of S. saprophyticus.Conclusion: These findings suggest that CoNS, especially <i>S. saprophyticus</i> strains, in raw pork meat could be a potential risk factor for staphylococcal food poisoning (SFP), and therefore, requires further investigation to elucidate the role of SEls in SFP and virulence of the pathogen. Our results also suggest that CoNS from raw pork meat may act as a source for transmission of antimicrobial resistance genes such as staphylococcal cassette chromosome <i>mec</i> and <i>tet</i>(K).

scholarly journals Profiles of coagulase-positive and -negative staphylococci in retail pork: prevalence, antimicrobial resistance, enterotoxigenicity, and virulence factors

2021 ◽  
Vol 34 (4) ◽  
pp. 734-742
Author(s):  
Gi Yong Lee ◽  
Soo-Jin Yang
Keyword(s):  
Antimicrobial Resistance ◽  
Zinc Chloride ◽  
Food Poisoning ◽  
Pork Meat ◽  
Coagulase Negative Staphylococci ◽  
Methicillin Resistant ◽  
Chloride Resistance ◽  
Antimicrobial Resistance Genes ◽  
Meat Samples ◽  
High Level

Objective: The present study aimed to investigate the occurrence and species of coagulasepositive staphylococci (CoPS) and coagulase-negative staphylococci (CoNS) in retail pork meat samples collected during nationwide monitoring. The staphylococcal isolates were characterized for antimicrobial and zinc chloride resistance and enterotoxigenic potential.Methods: A total of 260 pre-packaged pork meat samples were collected from 35 retail markets in 8 provinces in Korea for isolation of staphylococci. Antimicrobial and zinc chloride resistance phenotypes, and genes associated with the resistance phenotypes were determined on the isolates. Furthermore, the presence and distribution of 19 staphylococcal enterotoxin (SE) genes and enterotoxin-like genes among the pork-associated staphylococci were determined by multiplex polymerase chain reaction-based assays using the specific primer sets.Results: A total of 29 staphylococcal strains (29/260, 11.1%) were isolated from samples of retail pork meat, 24 (83%) of which were CoNS. The four CoNS species identified were <i>S. saprophyticus</i> (n = 16, 55%), <i>S. sciuri</i> (n = 3, 10%), <i>S. warneri</i> (n = 3, 10%), and <i>S. epidermidis</i> (n = 2, 7%). Among the 29 isolates, four methicillin-resistant CoNS (MR-CoNS; three <i>S. sciuri</i> and one <i>S. epidermidis</i>) and one methicillin-resistant CoPS (MR-CoPS; one <i>S. aureus</i>) were identified. In addition, a relatively high level of tetracycline (TET) resistance (52%) was confirmed in CoNS, along with a predominant distribution of <i>tet</i>(K). The most prevalent SEs were <i>sep</i> (45%), and <i>sen</i> (28%), which were carried by 81% of S. saprophyticus.Conclusion: These findings suggest that CoNS, especially <i>S. saprophyticus</i> strains, in raw pork meat could be a potential risk factor for staphylococcal food poisoning (SFP), and therefore, requires further investigation to elucidate the role of SEls in SFP and virulence of the pathogen. Our results also suggest that CoNS from raw pork meat may act as a source for transmission of antimicrobial resistance genes such as staphylococcal cassette chromosome <i>mec</i> and <i>tet</i>(K).

scholarly journals Antimicrobial Susceptibility Pattern of Gram-Negative Bacterial Isolates from Raw Chicken Meat Samples

2019 ◽  
Vol 6 ◽  
pp. 89-95
Author(s):  
Neha Gautam ◽  
Rojan Poudel ◽  
Binod Lekhak ◽  
Milan Kumar Upreti
Keyword(s):  
Antimicrobial Resistance ◽  
Pathogenic Bacteria ◽  
Chicken Meat ◽  
Food Microbiology ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Kathmandu Valley ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Meat Samples

Objectives: This research aims to study the microbial quality of chicken meat available in retail shop of Kathmandu Valley. Methods:  This Study was conducted from June to December 2018 in three different districts of Kathmandu Valley. Samples were collected in sterile plastic bags, labeled properly and stored in an icebox and transported to the Food Microbiology laboratory of Golden Gate International College.  During sample preparation, 25 grams of each sample was taken and transferred to sterile flasks containing 225 ml of buffered peptone water. Potential pathogenic Gram-negative bacteria were isolated by using respective selective media and identified by biochemical test. Antibiotic susceptibility profile of isolates was carried out by Kirby-Bauer disc diffusion method according to CLSI 2017 guideline. Results: Of total 81 chicken meat samples processed; 201 Gram negative bacteria were isolated.  E. coli (100%) was the dominant Gram-negative isolates, followed by Citrobacter spp (62.96%), Pseudomonas spp (40.74%), Proteus spp (19.75%), Salmonella spp (16.04%) and Klebsiella spp (8.64%) respectively. No any multidrug isolates were detected. Conclusion: The study showed that the raw chicken meat samples of Kathmandu valley was highly contaminated with Gram negative potential pathogenic bacteria. Antimicrobial resistance pattern shown by the isolates may indicates that there is not overuse of drug in animals and the less chance of risk of increasing antimicrobial resistance.

Occurrence and enterotoxin gene profiles of Staphylococcus aureus isolated from retail chicken meat

2020 ◽  
pp. 108201322098020
Author(s):  
Wilfred Ruban Savariraj ◽  
Narendra Babu Ravindran ◽  
Porteen Kannan ◽  
Veluru Appa Rao
Keyword(s):  
Staphylococcus Aureus ◽  
Biochemical Characterization ◽  
Chicken Meat ◽  
Gene Profiling ◽  
Enterotoxin Gene ◽  
Specific Pcr ◽  
Gene Profiles ◽  
Retail Outlets ◽  
Meat Samples ◽  
Retail Chicken Meat

The present study was conducted to evaluate the prevalence and enterotoxin gene profiles of Staphylococcus aureus isolated from 120 chicken meat marketed in retail outlets of Chennai, India. It was observed that total of 120 meat samples collected from different retail outlets, 66.67% (80/120) of the samples were positive for the presence of S. aureus based on biochemical characterization and species specific PCR based on thermonuclease gene ( nuc). Enterotoxin gene profiling of the isolates for 9 genes ( sea- sej) revealed that 52.50% (42/80) of the isolates in the present study were enterotoxigenic harboring either one or more gene. It was evident that majority of the isolates harbored seb, followed by seg, sei, sec, sed and sej either alone or in combination. None of the isolates harbored sea, see and seh either alone or in combination. The results of the study clearly indicated higher prevalence of enterotoxigenic S. aureus in retail meat marketed in Chennai, India indicating the potential of retail chicken meat to act as vehicle for food borne intoxication and a major public health threat.

scholarly journals Campylobacter jejuni from Canine and Bovine Cases of Campylobacteriosis Express High Antimicrobial Resistance Rates against (Fluoro)quinolones and Tetracyclines

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 691
Author(s):  
Sarah Moser ◽  
Helena Seth-Smith ◽  
Adrian Egli ◽  
Sonja Kittl ◽  
Gudrun Overesch
Keyword(s):  
Veterinary Medicine ◽  
Antimicrobial Resistance ◽  
Treatment Options ◽  
Tetracycline Resistance ◽  
Antimicrobial Treatment ◽  
Human Infection ◽  
Quinolone Resistance ◽  
European Program ◽  
Resistance Rates ◽  
Human Campylobacteriosis

Campylobacter (C.) spp. from poultry is the main source of foodborne human campylobacteriosis, but diseased pets and cattle shedding Campylobacter spp. may contribute sporadically as a source of human infection. As fluoroquinolones are one of the drugs of choice for the treatment of severe human campylobacteriosis, the resistance rates of C. jejuni and C. coli from poultry against antibiotics, including fluoroquinolones, are monitored within the European program on antimicrobial resistance (AMR) in livestock. However, much less is published on the AMR rates of C.jejuni and C. coli from pets and cattle. Therefore, C. jejuni and C. coli isolated from diseased animals were tested phenotypically for AMR, and associated AMR genes or mutations were identified by whole genome sequencing. High rates of resistance to (fluoro)quinolones (41%) and tetracyclines (61.1%) were found in C. jejuni (n = 29/66). (Fluoro)quinolone resistance was associated with the known point mutation in the quinolone resistance-determining region (QRDR) of gyrA, and tetracycline resistance was mostly caused by the tet(O) gene. These high rates of resistance, especially to critically important antibiotics in C. jejuni and C. coli, are worrisome not only in veterinary medicine. Efforts to preserve the efficacy of important antimicrobial treatment options in human and veterinary medicine have to be strengthened in the future.

Prevalence and Multidrug Resistance Pattern of Salmonella Carrying Extended-Spectrum β-Lactamase in Frozen Chicken Meat in Bangladesh

2020 ◽  
Vol 83 (12) ◽  
pp. 2107-2121
Author(s):  
MST. SONIA PARVIN ◽  
MD. MEHEDI HASAN ◽  
MD. YAMIN ALI ◽  
EMDADUL HAQUE CHOWDHURY ◽  
MD. TANVIR RAHMAN ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Foodborne Pathogen ◽  
Multidrug Resistant ◽  
Chicken Meat ◽  
Resistance Pattern ◽  
Salmonella Spp ◽  
Policy Makers ◽  
Disk Diffusion Test ◽  
Extended Spectrum ◽  
Meat Samples

ABSTRACT Salmonella is an important foodborne pathogen that causes public health problems globally, and the increase of antimicrobial resistance in Salmonella has intensified the problem. Chicken meat is an important reservoir and disseminator of Salmonella to humans. This study aimed at estimating the burden of Salmonella carrying extended-spectrum β-lactamase (ESBL) and their antimicrobial resistance pattern in 113 domestic frozen chicken meat samples purchased from supershops available in five divisional megacities of Bangladesh. The study also focused on the determination of β-lactamase–, and plasmid-mediated quinolone resistance–encoding genes. All samples were analyzed for the presence of Salmonella using selective media and PCR assay. Antimicrobial susceptibility test was done by disk diffusion test, and ESBL screening was performed by double-disk synergy tests. Resistance genes were detected using multiplex PCR. Of samples, 65.5% were positive for Salmonella spp., and, of these, 58.1% isolates were ESBL producers. All the isolates were multidrug resistant (MDR): 40.5% were resistant to both three to five and six to eight antimicrobial classes; 17.6% were resistant to 9 to 11 classes, and 1.4% isolates to 12 to 15 classes. The highest rates of resistance were observed against oxytetracycline (100%), followed by trimethoprim-sulfamethoxazole (89.2%), tetracycline (86.5%), nalidixic acid (83.8%), amoxicillin (74.3%), and pefloxacin (70.3%). Notably, 48.6% of isolates demonstrated resistance to imipenem. One (1.4%) isolate was possibly extensively drug resistant. All the isolates were positive for the blaTEM gene, 2.7% were positive for blaCTX-M-1, and 20.3% for blaNDM-1. The prevalence of qnrA and qnrS genes was 4.1 and 6.8%, respectively. This study shows that ESBL-producing Salmonella are widespread in frozen chicken meat in Bangladesh, which puts greater responsibility on food processors and policy makers to ensure food safety. HIGHLIGHTS

Antimicrobial Resistance Types and Genes in Salmonella enterica Infantis Isolates from Retail Raw Chicken Meat and Broiler Chickens on Farms

2006 ◽  
Vol 69 (1) ◽  
pp. 214-216 ◽  
Author(s):  
TETSUO ASAI ◽  
MICHIYO ITAGAKI ◽  
YUTAKA SHIROKI ◽  
MAKIKO YAMADA ◽  
MITSUO TOKORO ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Salmonella Enterica ◽  
Broiler Chickens ◽  
Point Mutations ◽  
Acid Resistance ◽  
Chicken Meat ◽  
Class 1 Integron ◽  
Antimicrobial Resistance Genes ◽  
Class 1 ◽  
Retail Chicken Meat

Salmonella enterica subsp. enterica serotype Infantis isolates from retail raw chicken meat (n = 98) and broiler chickens on farms (n = 70) were examined for antimicrobial susceptibility and antimicrobial resistance genes. A total of 15 antimicrobial resistance types, 14 in meat and 10 in broiler isolates, were identified, and 9 of the 15 types were indistinguishable between meat and broiler isolates. Resistance to both oxytetracycline and dihydrostreptomycin accounted for 94.0% of the resistance types in meat and broiler isolates, and each type harbored aadA1 within 1.0 kb of class 1 integron and tetA. Of nalidixic acid resistance types, point mutations at 87Asp (GAC) to Tyr (TAC) in the quinolone resistance-determining region of gyrA was detected in 10 of 13 meat isolates and at 87Asp to Asn (AAC) in four of seven broiler isolates. These findings suggest that the antimicrobial resistance of Salmonella Infantis in retail chicken meat predominantly originates from broiler chickens.

Sign in / Sign up

Export Citation Format

Share Document