Occurrence and enterotoxin gene profiles of Staphylococcus aureus isolated from retail chicken meat

2020 ◽  
pp. 108201322098020
Author(s):  
Wilfred Ruban Savariraj ◽  
Narendra Babu Ravindran ◽  
Porteen Kannan ◽  
Veluru Appa Rao
Keyword(s):  
Staphylococcus Aureus ◽  
Biochemical Characterization ◽  
Chicken Meat ◽  
Gene Profiling ◽  
Enterotoxin Gene ◽  
Specific Pcr ◽  
Gene Profiles ◽  
Retail Outlets ◽  
Meat Samples ◽  
Retail Chicken Meat

The present study was conducted to evaluate the prevalence and enterotoxin gene profiles of Staphylococcus aureus isolated from 120 chicken meat marketed in retail outlets of Chennai, India. It was observed that total of 120 meat samples collected from different retail outlets, 66.67% (80/120) of the samples were positive for the presence of S. aureus based on biochemical characterization and species specific PCR based on thermonuclease gene ( nuc). Enterotoxin gene profiling of the isolates for 9 genes ( sea- sej) revealed that 52.50% (42/80) of the isolates in the present study were enterotoxigenic harboring either one or more gene. It was evident that majority of the isolates harbored seb, followed by seg, sei, sec, sed and sej either alone or in combination. None of the isolates harbored sea, see and seh either alone or in combination. The results of the study clearly indicated higher prevalence of enterotoxigenic S. aureus in retail meat marketed in Chennai, India indicating the potential of retail chicken meat to act as vehicle for food borne intoxication and a major public health threat.

Molecular detection of enterotoxigenic Staphylococcus aureus isolated from mutton marketed in retail outlets of Chennai, India

2017 ◽  
Author(s):  
S. Wilfred Ruban ◽  
R. Narendra Babu ◽  
J. J. Abraham Robinson ◽  
Senthil Kumar ◽  
JT.M. A.P. Kumarasamy ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Detection ◽  
Toxin Gene ◽  
Specific Marker ◽  
Enterotoxin Gene ◽  
Gene Profiles ◽  
Retail Outlets ◽  
Meat Samples ◽  
Species Specific ◽  
Six Genes

The present study was aimed at detection of enterotoxigenic S. aureus in mutton marketed in retail outlets of Chennai. A total of 120 meat samples were collected from across Chennai for isolation of S. aureus and it was observed that 66.28 per cent of the samples were contaminated with S. aureus. The S. aureus count in mutton samples ranged from 1.8 x 102 to 4.9 x 104 CFU/ g with an overall average of 1.30 x 104 CFU/ g. All the isolates presumptively identified as S. aureus biochemically, amplified 181 bp product specific for nuc gene by PCR, which is species specific marker for S. aureus. Enterotoxin gene profiles (multiplex PCR) results revealed that 70.17 percent of the isolates were enterotoxigenic carrying only six genes (seb, sed, seg, seh, sei and sej) either alone or in combination, whereas none of these isolates harbored sea, sec and see. It was clear that seb (72.5 %) was the predominant enterotoxin gene followed by seg and sei, seh, sej and sed. Six different toxin gene profiles were exhibited by different isolates and majority of the isolates (55 %) carried two or more genes as compared to only one toxin gene.

scholarly journals Occurrence and antimicrobial susceptibility of Salmonella isolates from grilled chicken meat sold at retail outlets in Erbil City, Kurdistan region, Iraq

2019 ◽  
Vol 8 (2) ◽  
Author(s):  
Dhary A. Almashhadany
Keyword(s):  
Public Health ◽  
Health Hazard ◽  
Public Health Problem ◽  
Chicken Meat ◽  
Major Public Health Problem ◽  
Antibiotic Resistance Profile ◽  
Retail Outlets ◽  
Disk Diffusion Assay ◽  
Antibiotics Sensitivity ◽  
Meat Samples

Food borne salmonellosis is a major public health problem worldwide. This study aimed to detect the occurrence and antibiotics sensitivity of Salmonella species in grilled chicken meat sold at retail outlets in Erbil City, Kurdistan, Iraq. Two hundred and twenty-five (225) samples were aseptically collected from central and suburb retail outlets. For isolation of salmonellae, samples were cultured on selective media and tested for their susceptibility to common antibiotics by disk diffusion assay. The results revealed that the overall prevalence of Salmonella among grilled chicken meat samples was 7.1%. The isolates belonged to eight different serotypes of Salmonella. These include S. Typhimurium, S. Tennessee, S. Newport, S. Enteritidis, S. Anatum, S. Arizona, S. Muenchen, and S. Montevideo. The antibiotic resistance profile revealed a total resistance to Levofloxacin and total sensitivity to Cefotaxime, Amoxicillin, and Cefadroxil. This resistance among Salmonella may pose a public health hazard that requires effective precautions and response.

scholarly journals Antibiotic Resistance in Escherichia coli and Staphylococcus aureus from Retail Chicken Meat in Surabaya, Indonesia

2020 ◽  
Vol 3 (2) ◽  
pp. 80
Author(s):  
Annisa Aulia Maghfirani Sudarmadi ◽  
Subur Prajitno ◽  
Agung Dwi Wahyu Widodo
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Growth Promotion ◽  
Infectious Agent ◽  
Sampling Technique ◽  
Chicken Meat ◽  
Meat Sample ◽  
Meat Samples ◽  
And Staphylococcus Aureus

Introduction: Antimicrobial resistance is becoming a problem in public health. Zoonotic food-borne bacteria is infectious agent that can be transferred from animal to human through the food-producing animal we consume. Nowadays, antibiotic used for human and animal is not only to cure infection but also to aim animal’s growth promotion. It is known as non therapeutic antimicrobial agent (NTA) leading to antibiotic resistance. The third generation cephalosporins, cefotaxime, and also cefoxitin are included as important antibiotic for human. This study aims to identify the presence of cefotaxime-resistant Escherichia coli and cefoxitin-resistant Staphylococcus aureus isolated from chicken meat of both traditional and modern market in Surabaya.Methods: This is descriptive post test only experimental research. We used 8 samples of chicken meat from 4 different market using purposive sampling technique. We cultured Escherichia coli and Staphylococcus aureus from the chicken meat. Sensitivity test was done using Kirby-bauer disk-diffusion method.Results: All chicken meat sample bought from traditional market in Surabaya are contaminated by cefotaxime-sensitive Escherichia coli (n=4/4) while chicken meat sample bought from modern market are not contaminated by Escherichia coli (n=0/4). All chicken meat sample bought from traditional (n=4/4) are also contaminated by cefoxitin-sensitive Staphylococcus aureus. Half of chicken meat sample bought from modern market (n=2/4) are contaminated by cefoxitin-sensitive Staphylococcus aureus, while the other half (n=2/4) are contaminated by cefoxitin-resistant Staphylococcus aureus.Conclusion: Antibiotic resistance is found and all chicken meat samples have been highly contaminated with bacteria therefore food-processing should be done correctly.

scholarly journals Molecular Typing and Drug Resistance Patterns of Staphylococcus aureus Isolated From Raw Beef and Chicken Meat Samples

2020 ◽  
Vol 14 (5) ◽  
pp. 478-489
Author(s):  
Samaneh Farahmand ◽  
Mehri Haeili ◽  
Davood Darban-Sarokhalil ◽  
◽  
◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Drug Resistance ◽  
Molecular Typing ◽  
Chicken Meat ◽  
Resistance Patterns ◽  
Meat Samples

scholarly journals Comparative bacteriological analysis of frozen and fresh chicken meats sold around old site Bayero University Kano

2021 ◽  
Vol 14 (1) ◽  
pp. 258-262
Author(s):  
Habibu Usman Abdu ◽  
Aisha Mustapha Abubakar
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Species ◽  
Bacterial Count ◽  
Chicken Meat ◽  
Salmonella Spp ◽  
E Coli ◽  
Meat Samples ◽  
And Staphylococcus Aureus ◽  
Klebsiella Spp ◽  
Campylobacter Spp

Foodborne pathogens are serious public healthproblems that greatly affect the cost of food production and healthcare while poultry are often associated with foodborne disease outbreaks. This study aimed at investigating the occurrence and distribution of bacterial pathogens associated with chicken meats sold around Bayero University, Kano old site. A total of twenty (20) meat samples comprised of 10 frozen and ten freshly slaughtered chicken were purchased from different selling outlets around the study area. The samples were processed using standard cultural and biochemical procedures. The results of the study showed the mean aerobic mesophilic bacterial count of 5.93 x 106 CFU/g and 3.12 x 106  CFU/g for frozen and freshly slaughtered chicken meat samples respectively. The coliform counts were 420.1 and 347.7 CFU/100g for frozen and freshly slaughtered chicken meat samples respectively. The distribution of different bacterial species revealed E. coli (26.0%), Klebsiella spp (4.0%), Salmonella spp (19.5%), Campylobacter spp. (14.6%) and Staphylococcus aureus (30%) in frozen chicken meat samples while freshly slaughtered chicken meat samples revealed E. coli (21.0%), Klebsiella spp (8.9%), Salmonella spp (30%), Campylobacter spp. (15.0%) and Staphylococcus aureus (24.0%). This showed that frozen chicken meats were more contaminated than freshly slaughtered chicken meats which might be associated with poor handling and storage conditions.

Development of a Rapid On-Site Method for the Detection of Chicken Meat in Processed Ground Meat Products by Using a Direct Ultrafast PCR System

2020 ◽  
Vol 83 (6) ◽  
pp. 984-990 ◽  
Author(s):  
SUYEON SUL ◽  
MI-JU KIM ◽  
JUNG-MIN LEE ◽  
SUNG-YEON KIM ◽  
HAE-YEONG KIM
Keyword(s):  
Limit Of Detection ◽  
Meat Products ◽  
Accurate Method ◽  
Chicken Meat ◽  
Rrna Gene ◽  
Target Sequence ◽  
Ground Meat ◽  
Specific Pcr ◽  
Pcr Method ◽  
Meat Samples

ABSTRACT In this study, we developed a rapid on-site detection method by using direct ultrafast PCR coupled with a microfluidic chip to identify the presence of chicken meat in processed ground meat products. Chicken-specific PCR primer targeting mitochondrial 16S rRNA gene was newly designed, and its specificity was confirmed against 17 other animal species and 4 different chicken meat samples from different countries of origin. The sensitivity of the chicken-specific ultrafast PCR was 0.1 pg of chicken DNA. To evaluate the limit of detection of the direct ultrafast PCR method, different percentages of chicken meat mixed with pork or beef were prepared. The limit of detection of the direct ultrafast PCR method for the chicken meat–pork and chicken meat–beef mixtures was 0.1% for both raw meat and autoclaved meat. This method was used for 15 commercialized processed ground meat products. In this method, the target sequence was successfully amplified, and the presence of chicken meat in processed ground meat products was identified within approximately 25 min, including the time for sample preparation. Thus, our study shows that this developed direct ultrafast PCR method is a rapid and accurate method for on-site detection of chicken DNA in commercial food products. HIGHLIGHTS

scholarly journals Molecular Detection and Prevalence of Non-O157 Shiga Toxigenic Escherichia Coli in Selected Chicken Abattoirs and Retail Outlets in Osogbo, Osun State Nigeria

2021 ◽  
Author(s):  
Femi Ayoade ◽  
Judith Oguzie ◽  
Kazeem Akano ◽  
Testimony Olumade ◽  
Philomena Eromon ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Best Practices ◽  
Molecular Detection ◽  
E Coli ◽  
Retail Outlets ◽  
The Mean ◽  
Meat Samples ◽  
Retail Chicken Meat ◽  
Post Hoc ◽  
Specific Virulence

Abstract Three selected chicken abattoirs and two retail locations were studied to determine the prevalence and profile for Shiga toxin-producing Escherichia coli (STEC) present in abattoirs and retail (frozen) chicken carcasses in Osun state, Nigeria. Samples were plated on differential and selective media for the presence of E. coli. Multiplex PCR was used to check for specific virulence factors in the isolated E. coli samples. The mean colony count results showed that effluent water samples from the Ikirun slaughter slab type abattoir were the highest at 25 cfu/ml. A post hoc comparison showed that this value was significantly higher than that of the slaughtering table at Oluode-1 (P = 0.04) and retail chicken meat samples at Igbona (P = 0.01). These results suggest inadequate waste management and biological waste disposal systems and guides on best practices to limit the spread of potentially virulent pathogens into the runoff and groundwater.

scholarly journals Phenotypes, antibacterial-resistant profile, and virulence-associated genes of Salmonella serovars isolated from retail chicken meat in Egypt

2020 ◽  
Vol 13 (3) ◽  
pp. 440-445
Author(s):  
Amal Awad ◽  
Mayada Gwida ◽  
Eman Khalifa ◽  
Asmaa Sadat
Keyword(s):  
Virulence Genes ◽  
Antimicrobial Agents ◽  
Chicken Meat ◽  
Poultry Meat ◽  
Diffusion Method ◽  
Poultry Production ◽  
Production Chain ◽  
Salmonella Serovars ◽  
Meat Samples ◽  
Retail Chicken Meat

Aim: The present study was designed to investigate the occurrence and distribution of Salmonella serotypes in chicken meat samples, and to explore the susceptibility of the strains to antimicrobials, as well as their virulence-associated genes. Materials and Methods: Two-hundred retail chicken meat samples from different shops, as well as 25 stool specimens from retail shop workers, were included in the study. The collected samples were examined bacteriologically for the presence of salmonellae. Salmonella isolates were serotyped using a slide agglutination test for O and H antigens and were screened for the presence of five virulence genes (stn, pef, invA, sopB, and avrA) using a uniplex polymerase chain reaction assay and for their susceptibility to 18 antimicrobial agents using the disk diffusion method. Results: Thirty-one Salmonella isolates belonging to 12 different serovars were identified. Salmonella Enteritidis and Salmonella Kentucky were the dominant serovars (22.6% each). Salmonella isolates displayed a high antibiotic resistance against erythromycin, sulfamethoxazole/trimethoprim, doxycycline, cephalexin, cefaclor, tetracycline, polymyxin B, cefuroxime, vancomycin, and streptomycin. All Salmonella isolates exhibited multidrug resistance (MDR) and demonstrated different virulence genes. The majority of Salmonella serovars (87.1%) harbored sopB gene, 54.8% carried avrA and pef genes, while all isolates carried invA and stn genes. Conclusion: The presence of virulent MDR Salmonellae in raw chicken meat could allow the possibility of transmission of these resistant serovars to humans. Therefore, strict hygienic measures should be followed on the whole poultry production chain to decrease the potential transmission of Salmonella infection from poultry meat to humans.

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