Screening of Antioxidative Properties and Inhibition of Inflammation-Linked Enzymes by Aqueous and Ethanolic Extracts of Plants Traditionally Used in Wound Healing in Poland

A wide range of plant-derived preparations have been used against skin inflammatory disorders and as wound healing agents in traditional medicine. The purpose of the study was to determine the antioxidant activity of aqueous and 70% ethanolic extracts from eleven species of plants traditionally used in Poland to treat inflammatory skin diseases. The ability of extracts to scavenge 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), hydrogen peroxide (H2O2), and superoxide anion (O2•−), was studied. In non-cellular studies, an analysis of the anti-inflammatory effect on the activity of enzymes, such as hyaluronidase (HYAL) and lipoxygenase (LOX), was also performed. The chemical profiles of the most active extracts were achieved by applying the UHPLC-DAD-MSn method, and the sum of polyphenols in all tested extracts was determined by the colorimetric method with the Folin–Ciocalteu reagent. The scope of the extracts’ influence on enzyme activity was significantly lower than their antioxidant activity. All extracts have shown high activity in free radical scavenging against DPPH. The ethanolic extracts have shown high potential to scavenge H2O2. The study of composition showed that the main components of the tested extracts were flavonoids, such as luteolin, apigenin, kaempferol, and quercetin derivatives, as well as caffeoylquinic acids, caffeic acid, and its conjugates.

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IN VITRO ANTIOXIDANT ACTIVITY AND WOUND HEALING ACTIVITY OF WHEATGRASS BY 1,1- DIPHENYL, 2 PICRYLHYDRAZYL METHOD

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i12.27705 ◽

2018 ◽

Vol 11 (12) ◽

pp. 170

Author(s):

Veermaneni Alekhya ◽

Thiyagarajan Deepan ◽

Magharla Dasaratha Dhanaraju

Keyword(s):

Antioxidant Activity ◽

Wound Healing ◽

Skin Diseases ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Wheat Plant ◽

Wide Range ◽

Wound Healing Activity ◽

In Vitro Antioxidant Activity

Objective: This study was designed to evaluate in vitro antioxidant activity and wound healing activity in Triticum aestivum (wheat grass).Methods: T. aestivum commonly known as Wheatgrass had a wide range of health benefits among the young grass of common wheat plant components includes chlorophyll, flavonoids, and Vitamins A, C, and E. Wheatgrass is used in Folklore medicine for treatment of skin diseases and wound healing. In our present study, petroleum ether, ethanol and aqueous extracts of T. aestivum have been evaluated for in vitro antioxidant activity and wound healing activity by 1,1- diphenyl, 2 Picrylhydrazyl radical scavenging activity, and Chick chorioallantoic method, respectively.Results: The results of both the assay showed that all the extracts of T. aestivum have significant antioxidant and wound healing activity on dose-dependent manner.Conclusion: The wheatgrass has antioxidant and wound healing activity.

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Bioactive Compounds of Selected Fruit Juices

Natural Product Communications ◽

10.1177/1934578x0900400505 ◽

2009 ◽

Vol 4 (5) ◽

pp. 1934578X0900400 ◽

Cited By ~ 1

Author(s):

Aneta Wojdyłto ◽

Jan Oszmiański

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Fruit Juice ◽

Biological Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Sea Buckthorn ◽

Quercetin Derivatives ◽

Reducing Ability ◽

Wide Range

Fruits are one of the most important sources of phenolic compounds in our diet. Many of these compounds exhibit a wide range of biological activities, especially antioxidant activity. The objective of this study was to determine the composition and antioxidant activity of phenolic compounds found in fruit juice obtained from buckthorn, flowering quince, rowanberry and hawthorn. Hydroxycinnamic acids (neochlorogenic and chlorogenic acid) and procyanidins were the main phenolics in rowanberry juice. The highest level of flavonols (quercetin derivatives) was found in sea buckthorn. All juices showed strong free-radical scavenging activity against ABTS•+ radicals and ferric reducing ability measured by the FRAP method. The strongest antioxidant activity was found in rowanberry juice, but the lowest was measured in hawthorn and sea buckthorn juices.

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Evaluation of Antioxidant and Wound Healing Potentials ofSphaeranthus amaranthoidesBurm.f.

BioMed Research International ◽

10.1155/2013/607109 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 34

Author(s):

R. Geethalakshmi ◽

C. Sakravarthi ◽

T. Kritika ◽

M. Arul Kirubakaran ◽

D. V. L. Sarada

Keyword(s):

Antioxidant Activity ◽

Wound Healing ◽

Skin Diseases ◽

Methanolic Extract ◽

Radical Scavenging ◽

Silver Sulfadiazine ◽

Wound Contraction ◽

Total Phenolic ◽

Flavonoid Fraction ◽

Wound Healing Activity

Background.Sphaeranthus amaranthoidescommonly known as sivakaranthai is used in folklore medicine for the treatment of skin diseases.Methods. The antioxidant activity of the extract and its fraction was evaluated by using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, total antioxidant capacity, and total phenolic content. The tested plant extracts showed variable degrees of antioxidant activity. In the present study, methanolic extract of the whole plant ofS. amaranthoidesand a flavonoid fraction obtained from column chromatography were studied for wound healing activity by incorporating the sample in simple ointment base. Wound healing activity was studied in excision wound model in rats, following which, wound contraction, period of epithelization, hydroxyproline content, and collagen levels in the scab were studied.Results. Methanolic extract showed the highest antioxidant effect (72.05%) and diethyl ether extract has the least (29.34%) compared to the standard (74.53%). Treatment of wound with ointment containing 5% (w/w) methanolic extract and 5% (w/w) flavonoid fraction exhibited better wound healing activity than positive control (silver sulfadiazine). Finally, histopathology studies conformed wound healing activity inSphaeranthus amaranthoides. The methanolic extract and flavonoid fraction exhibited good wound healing activity probably due to the presence of phenolic and flavonoid constituents. The methanolic extract and flavonoid fraction significantly enhanced the rate of wound contraction and the period of epithelialization comparable to silver sulfadiazine.

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Aktywność przeciwutleniająca i przeciwbakteryjna miodu, propolisu oraz pyłku kwiatowego

Postępy Fitoterapii ◽

10.25121/pf.2020.21.3.123 ◽

2020 ◽

Vol 21 (3) ◽

Author(s):

Magdalena Woźniak ◽

Lucyna Mrówczyńska ◽

Anna Sip ◽

Marta Babicka ◽

Tomasz Rogoziński ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Pathogenic Bacteria ◽

Radical Scavenging Activity ◽

Colorimetric Method ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Total Content ◽

Diffusion Method ◽

Bacterial Strains

Introduction. Honey, propolis and pollen belong to bee products that have beneficial biological properties. These products exhibit e.g. antibacterial, antifungal and antioxidant properties. Due to biological activity and natural origin, bee products are used, e.g. in the food industry, cosmetology and pharmacy. Aim. The aim of the study was to compare the antioxidant and antibacterial activity of honey, propolis and pollen from an apiary located in Wielkopolska Province. Material and methods. Honey, propolis and pollen used in this study came from the same apiary located in Wielkopolska Province. The antioxidant potential of bee products was evaluated applying DPPH· free radical scavenging activity assay. The antimicrobial activity of the tested bee products was determined by the point-diffusion method against 13 strains of pathogenic and potentially pathogenic bacteria. In addition, the total content of phenolic compounds in honey, propolis and pollen was determined by the colorimetric method. Results. Propolis exhibited higher antioxidant activity, in comparison to honey and pollen. The antiradical activity of propolis was equal to 80% approx. activity of Trolox, the standard antioxidant. Among tested bee products, propolis was characterized by the highest total phenols content. In addition, honey, propolis and pollen showed antagonistic activity against tested bacterial strains. Conclusions. The obtained results indicate that among the tested bee products of native origin, i.e. honey, propolis and pollen, propolis characterized by the highest antioxidant activity and the total content of phenolic compounds. In addition, all bee products showed bactericidal activity against the tested bacterial strains.

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RELATIONSHIP BETWEEN ANTIOXIDANT PROPERTIES AND NUTRITIONAL COMPOSITION OF SOME GALACTOPOIETICS HERBS USED IN INDONESIA: A COMPARATIVE STUDY

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2016v8i12.14964 ◽

2016 ◽

Vol 8 (12) ◽

pp. 236 ◽

Cited By ~ 4

Author(s):

Ade Chandra Iwansyah ◽

Rizal M. Damanik ◽

Lilik Kustiyah ◽

Muhammad Hanafi

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Total Phenolics ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Nutritional Composition ◽

Total Flavonoids ◽

Wide Range ◽

Phenolics Content

Objective: The objectives of this research were to evaluate and compare the antioxidant activity; phenolics and flavonoids contents; and chemical composition of some galactopoietic herbs used in Indonesia and to find a possible relationship between antioxidant activity; phenolics, flavonoids and chemical composition of these plants.Methods: A wide range of analytical parameters was studied, including yield; pH; water activity (aw); total color difference; phenolics and flavonoids content; moisture; ash; protein; fat; carbohydrate; fiber; calcium; zinc; magnesium; potassium; iron content; and the data were analyzed by partial least squares (PLS) regression analysis and principal component analysis (PCA) to evaluate the correlation between the parameters and classification of the plants. Folin–cioceltaeu assay, aluminum chloride assay and DPPH scavenging assay were used to evaluate of total phenolics content and free radical scavenging activity of the ethanolic extract of galactopoietic herbs.Results: All of the galactagogue herbs, viz. Plectranthus amboinicus (Lour.) (PA), Sauropus androgynus (SA), Moringa oleifera (MO), and Carica papaya leaves (CP) exhibited good results of TPC, TFC and antioxidant activity. MO contained the highest average of total phenolics content (53.89 mg GAE/g). SA with a ratio of total flavonoids to total phenolics (TF/TP) which is 1.59 contained the highest average of total flavonoids and antioxidant activity (IC50), 43.63 mg QE/g and 126.13 μg/ml, respectively, followed by MO>CA>PA. Based on PLS and PCA analysis, antioxidant activity correlated with fat, protein, phenolics content, flavonoids content and pH, but only the total flavonoids content correlated strongly with antioxidant activity.Conclusion: This provides useful data about the studied relationships and chemical patterns in some galactopoietic herbs used and provides a helpful tool for understanding much more about the interpretation of results from the experiments and the mechanism of biosynthesis in plants.

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POLYPHENOL CONTENT AND ANTIOXIDANT POTENTIAL OF Allophylus edulis (A. St.-Hil. et al.) Hieron. ex Niederl. AND Cupania vernalis Cambess. (SAPINDACEAE)

Revista Árvore ◽

10.1590/1806-908820210000007 ◽

2021 ◽

Vol 45 ◽

Author(s):

Andréa Michel Sobottka ◽

Elisandra Tessaro ◽

Suelen Maier da Silva ◽

Marina Pedron ◽

Lara Tortini Seffrin

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Skin Diseases ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Ethyl Acetate Fraction ◽

Antioxidant Potential ◽

Total Phenolic ◽

Butanol Fraction ◽

Degraded Ecosystems

ABSTRACT Allophylus edulis and Cupania vernalis (Sapindaceae) are Brazilian native trees used as medicinal plants for the treatment of respiratory, digestive, circulatory, and skin diseases. Ubiquitously distributed in the Brazilian territory, these species are indicated for mixed plantations aimed at the recovery of degraded ecosystems. In this study, the total phenolic content (TPC) and total flavonoid content (TFC), and the antioxidant activity of extracts and fractions obtained from A. edulis and C. vernalis leaves were assessed. The TPC and TFC was determined spectrophotometrically. Antioxidant activity was evaluated through radical scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH). The extracts were obtained by two methods: maceration (method 1) and Soxhlet (method 2). Solvents of increasing polarity (hexane, dichloromethane, ethyl acetate, and n-butanol) were used to obtained the fractions. The results showed that the ethyl acetate fraction from A. edulis, obtained from the maceration method, had the highest TPC (442.0 ± 18.2 mg GAE g-1) and TFC (58.1 ± 0.4 mg RUE g-1), and antioxidant activity (EC50 = 43.6 ± 2.6 µg mL-1). By C. vernalis, superior results were obtained with the n-butanol fraction (TPC = 126.1 ± 5.8 mg GAE g-1, TFC = 37.7 ± 0.6 mg RUE g-1). The highest antioxidant potential was found in the crude hydroalcoholic extract (EC50 = 816.1 ± 50.9 µg mL-1) and butanol fraction (1,156.4 ± 3.8 µg mL-1). The results of this study show that the fractions obtained by maceration and liquid-liquid partition with more polar solvents (ethyl acetate and n-butanol) are the richest in TPC and TFC, and presented the greater antioxidant activity. Comparing the two plants, A. edulis showed the best results, with a high content of TPC, TFC, and antioxidant potential, and therefore may be used to treat diseases related to oxidative stress.

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Mycochemical composition and antioxidant potential of Macrolepiota africana (Heim) Heinem. (Agaricaceae), an edible mushroom from Gabon

Open Access Research Journal of Life Sciences ◽

10.53022/oarjls.2021.2.1.0134 ◽

2021 ◽

Vol 2 (1) ◽

pp. 028-033

Author(s):

Hugues Calixte Eyi Ndong ◽

André Ledoux Njouonkou

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Activity Index ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Edible Mushroom ◽

Mushroom Species ◽

Chemical Screening ◽

Ethanolic Extracts ◽

Pharmacological Potential

Macrolepiota africana is a fungus used as food in Gabon for its culinary properties. However, its nutritional value, mycochemical contents, antioxidant properties and health potential still unknown. The present study investigated the phytochemicals and antioxidant properties of this mushroom species. This chemical screening was followed by a study of the antioxidant activity and a prediction of additional pharmacological activities of M. africana. Using standard methodology, the mycochemical analyses were carried out on aqueous, hydro-ethanolic and ethanolic fungi extracts. The antioxidant activity of the mushroom extracts was determined using DPPH radical scavenging assay. Apart from Digitoxigenine, flavonol and gitoxigenine that were not found in any of the extracts, all other tested mycochemical were found in atleast one of the extracts. Alkaloids, flavonoids, polyphenols, oses and holosides, proanthocyanidins and coumarins were found in all extracts at different intensity. Saponosids, sterols and triterpenes, tannins gallics, reducing sugar, anthracenosides and digitoxine were found in two extracts while tannins catechics, flavone and gitoxine were detected only in one extract. The dosage of phenolic compounds confirmed the richness of this fungus in total polyphenols, its moderate richness in proanthocyanidins, the lack of flavonoids in the aqueous extract and the moderate richness of the hydro-ethanolic and ethanolic extracts in flavonoids. Regarding the antioxidant activities, the results obtained for the DPPH trapping test showed that the different extracts had low to moderate antioxidant activity with antioxidant activity index (IAA) ranged 0.29 to 0.97 respectively in aqueous and ethanolic extracts. The presence of these mycochemical compounds along with the identified antioxidant activities shows that this M. africana have some pharmacological potential.

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PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF INDIGOFERA TINCTORIA L.

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i6.20060 ◽

2018 ◽

Vol 11 (6) ◽

pp. 136

Author(s):

Chandrasekaran Swaminathan

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Diffusion Method ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Root Extracts ◽

Wide Range ◽

Indigofera Tinctoria

Objective: The present study was designed to evaluate the phytochemical composition and antibacterial and antioxidant potential of methanolic leaf and root extracts of Indigofera tinctoria L.Methods: Phytochemical analysis was done using standard methods. The methanolic leaf and root extracts of the plant were tested against Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, Enterobacter aerogenes, and Salmonella paratyphi B by cup-plate agar diffusion method. The free radical scavenging activities of the methanol extracts of leaves and roots were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay.Results: Phytochemical screening revealed the presence of carbohydrates, reducing sugars, alkaloids, saponins, phenolic compounds, and flavonoids in methanolic leaf and root extracts. Methanolic leaf extracts of the plant exhibited antibacterial activity against a wide range of bacteria, but the root extracts failed to inhibit the tested bacterial pathogens. The antioxidant activity determination revealed that at 100 μg/ml, methanolic root extracts had the highest antioxidant activity (89.10%) on DPPH free radicals followed by methanolic leaf extracts (46.74%).Conclusion: The results of the present study conclude that the studied plant possesses broad-spectrum antibacterial and antioxidant properties and may act as a potent antioxidant for biological systems susceptible to free radical-mediated reactions.

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Modification of Hydroxypropyltrimethyl Ammonium Chitosan with Organic Acid: Synthesis, Characterization, and Antioxidant Activity

Polymers ◽

10.3390/polym12112460 ◽

2020 ◽

Vol 12 (11) ◽

pp. 2460

Author(s):

Yingqi Mi ◽

Wenqiang Tan ◽

Jingjing Zhang ◽

Zhanyong Guo

Keyword(s):

Antioxidant Activity ◽

Organic Acids ◽

Organic Acid ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Scavenging Activity ◽

Chitosan Derivatives ◽

13C Nuclear Magnetic Resonance ◽

Wide Range

A novel and green method for the preparation of chitosan derivatives bearing organic acids was reported in this paper. In order to improve the antioxidant activity of chitosan, eight different hydroxypropyltrimethyl ammonium chitosan derivatives were successfully designed and synthesized via introducing of organic acids onto chitosan by mild and non-toxic ion exchange. The data of Fourier Transform Infrared (FTIR), 13C Nuclear Magnetic Resonance (NMR), 1H NMR, and elemental analysis for chitosan derivatives indicated the successful conjugation of organic acid salt with hydroxypropyltrimethyl ammonium chloride chitosan (HACC). Meanwhile, the antioxidant activity of the chitosan derivatives was evaluated in vitro. The results indicated that the chitosan derivatives possessed dramatic enhancements in DPPH-radical scavenging activity, superoxide-radical scavenging activity, hydroxyl radical scavenging ability, and reducing power. Furthermore, the cytotoxicity of the synthesized compounds was investigated in vitro on L929 cells and showed low cytotoxicity. Thus, the enhanced antioxidant property of all novel chitosan products might be a great advantage, while applied in a wide range of applications in the form of antioxidant in biomedical, food, and cosmetic industry.

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Antibacterial, Antioxidant Activity of Ethanolic Plant Extracts of Some Convolvulus Species and Their DART-ToF-MS Profiling

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2017/5694305 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9 ◽

Cited By ~ 9

Author(s):

Asma’a Al-Rifai ◽

Ahmad Aqel ◽

Tarfah Al-Warhi ◽

Saikh M. Wabaidur ◽

Zeid A. Al-Othman ◽

...

Keyword(s):

Skin Diseases ◽

Antioxidant Activities ◽

Biological Activities ◽

Folk Medicine ◽

Colorimetric Method ◽

Radical Scavenging ◽

Direct Analysis ◽

Analytical Technique ◽

Flight Mass Spectrometry

Convolvulus austroaegyptiacus Abdallah & Sa’ad (CA) and Convolvulus pilosellifolius Desr. (CP) are commonly used in the Saudi Arabia folk medicine. They are potent in treating the ulcers and skin diseases. The lack of information about their biological activities led us to investigate the possible biological activities by determination of antibacterial and antioxidant activities of total ethanolic extracts and various fractions. Total flavonoid contents of the plants were determined by colorimetric method while total phenols were determined by using Folin-Ciocalteu method. In vitro antibacterial activity was studied against E. coli, P. aeruginosa, and B. subtilis, and the total antioxidant capacity was evaluated by radical scavenging method. IC50 were found to be 21.81, 17.62, and 3.31 μg/mL for CA, CP, and vitamin C, respectively, while the lowest MIC value of 0.25 mg/mL was recorded with CP extract against B. subtilis. Around 21 compounds are tentatively elucidated from both plants using rapid, simple, and high-resolution analytical technique for chemical profiling of natural compounds by direct analysis in real-time of flight-mass spectrometry, of which 17 were not isolated or reported previously.

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