scholarly journals UHPLC-ESI-QTOF-MS/MS Metabolite Profiling of the Antioxidant and Antidiabetic Activities of Red Cabbage and Broccoli Seeds and Sprouts

Antioxidants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 852
Author(s):  
Simon-Okomo Aloo ◽  
Fred-Kwame Ofosu ◽  
Eric-Banan-Mwine Daliri ◽  
Deog-Hwan Oh
Keyword(s):  
Metabolite Profiling ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Activity Levels ◽  
Red Cabbage ◽  
Saponin Content ◽  
Potent Inhibition ◽  
Ethanol Extracts ◽  
Age Inhibition

The antioxidant and antidiabetic properties and metabolite profiling of ethanol extracts of red cabbage (RC) and broccoli (BR) seeds and sprouts were investigated in this study. The total phenolic, flavonoid, and saponin contents were in the ranges of 385.4–480.4 mg FAE/100 g, 206.9–215.6 mg CE/100 g, and 17.8–27.0 mg soysaponin BE/100 g, respectively. BR seed had the highest total phenolic (480.4 mg FAE/100 g) and flavonoid (216.9 mg CE/100 g) contents, whereas BR sprout had the highest saponin content (27.0 soysaponin BE/100g). RC sprout demonstrated the highest antioxidant capacity, with DPPH and ABTS radical scavenging activity levels of 71.5% and 88.5%, respectively. Furthermore, BR and RC sprouts showed the most potent inhibition against α-glucosidase (91.32% and 93.11%, respectively) and pancreatic lipase (60.19% and 61.66%, respectively). BR seed (60.37%) demonstrated the lowest AGE inhibition. A total of 24 metabolites, predominantly amino acids and phenolic compounds, were characterized using UHPLC-QTOF-MS/MS. Germination not only improved the levels of metabolites but also resulted in the synthesis of new compounds. Therefore, these findings show that germination effectively enhanced the functional properties and metabolite profiles of broccoli and red cabbage seeds, making their sprouts more applicable as functional ingredients.

scholarly journals Antioxidant, Cytotoxic Activities and Total Phenolic Content of Four Indonesian Medicinal Plants

2012 ◽  
Vol 2 (4) ◽  
Author(s):  
Waras Nurcholis ◽  
Bambang Pontjo Priosoeryanto ◽  
Edy Djauhari Purwakusumah ◽  
Takeshi Katayama ◽  
Toshisada Suzuki
Keyword(s):  
Free Radical ◽  
Medicinal Plants ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Ic50 Values ◽  
Ethanol Extracts

The crude ethanol extracts of four Indonesian medicinal plants namely Curcuma xanthorrhiza Roxb.,Phyllanthus niruri Linn., Andrographis paniculata Ness., and Curcuma aeruginosa Roxb. wereexamined for their antioxidant (radical scavenging) activity using 2, 2-diphenyl-2-picrylhydrazyl(DPPH) free radical and cytotoxicity using brine shrimp lethality test (BSLT). The total phenoliccontent was used the Folin-Ciocalteu method. IC50 values for DPPH radical scavenging activityranged from 14.5 to 178.5 μg/ml, with P. niruri having the lowest value and therefore the mostpotent, and C. aeruginosa having the highest value. LC50 values for BSLT ranged from 210.3 to593.2 μg/ml, with C. xanthorrhiza and A. paniculata having the lowest and highest values,respectively. The total phenolic content of the Indonesian plants ranged from 133.0 ±3.7 to863.3±54.7 mg tannic acid equivalent per 1 g extract, with C. aeruginosa and P. niruri having thelowest and highest values, respectively. A positive correlation between free radical scavengingactivity and the content of phenolic compounds was found in the four of Indonesian medicinal plants.

scholarly journals Keeping quality of beef sausage using ethanolic extract of gamma-irradiated pomegranate peel powder

2016 ◽  
pp. 29-50 ◽  
Author(s):  
Ibrahim Mohammed ◽  
Fawzy Hassanien ◽  
Sahy El ◽  
Mohammed Afify ◽  
Abdeldaiem Mohammed
Keyword(s):  
Antibacterial Activity ◽  
Shelf Life ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Punica Granatum ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Ethanol Extracts ◽  
Gamma Irradiated

The present study aims to evaluate the antioxidant and antibacterial activity of ethanol extracts from gamma-irradiated pomegranate (Punica granatum) peel powder (PE) at the dose levels of 0, 3, 6, and 9 kGy. The ?ntioxidant activity of the extracts was estimated using the radical scavenging activity against 2,2?-diphenyl-1-picrylhydrazyl (DPPH?), ?-carotene/linoleic acid bleaching system, and ferric reducing antioxidant power (FRAP). Antibacterial activity of the extracts was assessed against Bacillus cereus, Staphylococcus aureus, Escherichia coli, Klebsilla penumoneae, Pseudomonas aeruginosa, and Salmonella typhumurium. The results showed that PE treated with 6 kGy had a higher content of total phenolic compounds (TPC), total flavonoid compounds (TFC), and antioxidant activity. Hence, the PE of 6 kGy-irradiated peels were selected to be added to beef sausage. Different concentrations of 6 KGy-irradiated PE were applied to improve beef sausage hygienic quality and extend the shelf life during cold storage (4?C). The results indicated that when 12 mL of the extract were added to one kg of beef sausage, the shelf-life of the sausage was extended from 15 days to 50 days (at 4?C), compared with the control, without changes of the microbiological, chemical, and sensory attributes.

scholarly journals IN VITRO ANTI-DIABETIC AND ANTI-OXIDANT ACTIVITIES OF ETHANOL EXTRACT OF TINOSPORA SINENSIS

2017 ◽  
Vol 9 (2) ◽  
pp. 42 ◽  
Author(s):  
Anindita Banerjee ◽  
Bithin Maji ◽  
Sandip Mukherjee ◽  
Kausik Chaudhuri ◽  
Tapan Seal
Keyword(s):  
Plant Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Inhibitory Activities ◽  
Ethanol Extracts

Objective: The aim of the present study was to evaluate the alpha (α)-amylase and alpha (α)-glucosidase inhibitory activities and in vitro antioxidant activities of the 80 % aqueous ethanol extracts of Tinosporasinensis Lour (Merr.).Methods: The 80% aq. ethanol extract of the plant was prepared. The plant extract was examined for its antioxidant activity by using free radical 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging method, ABTS radical scavenging ability, reducing power capacity, estimation of total phenolic content, flavonoid content and flavonol content. Different concentrations (2, 4, 8,10and 15 μg/ml) of the extract was subjected to α-amylase inhibitory and α-glucosidase inhibitory activities and IC50were calculated.Results: The study revealed that the different concentrations of the plant extract possessed a very good amount of total phenolics, flavonoid and flavonol and exhibited potent radical scavenging activity using DPPH and ABTS as a substrate. The ethanol extracts exhibited significant α-amylase and α-glucosidase inhibitory activities with an IC50 value1.093µg and 1.04µg dry extract respectively and well compared with standard acarbose drug.Conclusion: Thus, it could be concluded that due to the presence of antioxidant components the plant extracts could be used for the treatment of hyperglycemia, diabetes and the related condition of oxidative stress. This knowledge will be useful in finding more potent components from the natural resources for the clinical development of antidiabetic therapeutics.

scholarly journals Enhancement of Minor Ginsenosides Contents and Antioxidant Capacity of American and Canadian Ginsengs (Panax quinquefolius) by Puffing

Antioxidants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 527 ◽  
Author(s):  
Min-Soo Kim ◽  
Sung-Joon Jeon ◽  
So Jung Youn ◽  
Hyungjae Lee ◽  
Young-Joon Park ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Value Added ◽  
Panax Quinquefolius ◽  
Total Phenolic ◽  
Maillard Reaction Products ◽  
Scavenging Activity ◽  
Reaction Products ◽  
Saponin Content

The effects of puffing on ginsenosides content and antioxidant activities of American and Canadian ginsengs, Panax quinquefolius, were investigated. American and Canadian ginsengs puffed at different pressures were extracted using 70% ethanol. Puffing formed a porous structure, inducing the efficient elution of internal compounds that resulted in significant increases in extraction yields and crude saponin content. The content of minor ginsenosides (Rg2, Rg3, compound K) increased with increasing puffing pressure, whereas that of major ginsenosides (Rg1, Re, Rf, Rb1, Rc, Rd) decreased, possibly due to their deglycosylation and pyrolysis. Furthermore, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging activity, total phenolic content, total flavonoid content, amount of Maillard reaction products, and acidic polysaccharides content increased with increasing puffing pressure, but 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity did not. There was no substantial difference in the results between puffed American and Canadian ginsengs. Consequently, these results suggest that puffing can be a promising novel technology for processing P. quinquefolius to achieve higher levels of minor ginsenosides and obtain value-added products.

scholarly journals Multi-experimental characterisation of grape skin extracts

2009 ◽  
Vol 26 (Special Issue) ◽  
pp. S43-S48 ◽  
Author(s):  
M. Šťavíková L Polovka ◽  
B. Hohnová ◽  
J. Zemanová
Keyword(s):  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Cation Radical ◽  
Principal Component ◽  
Total Phenolic ◽  
Grape Skin ◽  
Grape Skins ◽  
Ethanol Extracts ◽  
The Individual

Grape skins contain a plenty of different flavonoids, most of them revealing significant antioxidant properties. In this contribution, a complex study is presented of grape skin ethanol extracts, prepared from grape skins of two vine grape varieties, Svatovavřinecké (St. Laurent) and Alibernet. Extracts were prepared from two different amounts of lyophilised grape skin powders using the pressurised fluid extraction (PFE). The antioxidant activity of the extracts was tested by EPR spectroscopy in Fenton system generating reactive radicals (•OH, O<sub<sup>–</sup>•, •R) followed by spin trapping technique. In addition, radical scavenging activity of the extracts was assessed applying 2,2-diphenyl-1-picrylhydrazyl (•DPPH) free radical and 2,2’-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) cation radical (ABTS•><sup>+></sup>) assays. Total phenolic content (TPC) of the individual extracts and their tristimulus colour values (CIE Lab) were evaluated, using an UV-VIS spectrophotometer. All the data obtained were subsequently correlated and discriminated, using the multivariate statistics, involving the canonical discriminant analysis, principal component analysis, and canonical correlation analysis. Results obtained indicated that PFE is a suitable extraction technique, only slightly influencing antioxidant ability as well as composition of the so-prepared extracts. The influence of extraction conditions on the entire monitored characteristics was insignificant.

scholarly journals Evaluation of phytochemicals and antioxidant activity of gamma irradiated quinoa (Chenopodium quinoa)

2020 ◽  
Author(s):  
M. A. Abdelaleem ◽  
K. R. A. Elbassiony
Keyword(s):  
Antioxidant Activity ◽  
Gamma Irradiation ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Antioxidant Power ◽  
Saponin Content ◽  
Irradiation Treatment

Abstract Phytochemical and antioxidant activity of quinoa flour was evaluated after subjected to gamma irradiation processes at dose 3 and 6 kGy. Both non-irradiated and irradiated quinoa samples were subjected to successive extractions in ethanol solvent. The antioxidant activity after gamma irradiation treatment was investigated via Ferric reducing antioxidant power (FRAP) and radical-scavenging activity (RSA) using 2,2-diphenyl-1-picrylhydrazyl (DPPH).Total phenolic and flavonoid content were analyzed using Folin–Ciocalteu micro-method, aluminium chloride (AlCl3) method and High Performance Liquid Chromatography (HPLC). As well as, effect of irradiation treatment on saponin was also evaluated. Irradiation treatment showed slight differences in the saponin content after exposure to 3 and 6 kGy. Irradiation process enhanced both total phenolic content (TPC) and Total flavonoid content (TFC), TPC were 34.52 and 30.92 mg Gallic Acid Equivalent (GAE)/100g compared to 26.25 mg GAE/100g in non-irradiated quinoa. TFC were 67.44 and 62.89 mg Quercetin Equivalents (QE)/100g compared to 53.15 mg QE/100g. Irradiation dose 3 kGy significantly (p> 0.05) decreased the IC50 as DPPH-RSA and increased the FRAP.

scholarly journals Phytochemical and Antioxidant Activities of Cucumis africanus L.f.:: A Wild Vegetable of South Africa

2019 ◽  
Vol 24 ◽  
pp. 2515690X1983639 ◽  
Author(s):  
Taiwo Oluwafunmilola Abifarin ◽  
Anthony Jide Afolayan ◽  
Gloria Aderonke Otunola
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Acetone Extract ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Phytochemical Content ◽  
Spectrophotometric Methods ◽  
Saponin Content ◽  
Radical Scavenging Ability

Objective. To evaluate the phytochemical content and antioxidant potential of the acetone, aqueous, and methanol extracts of the fruit, leaf, and root of Cucumis africanus L.f. Methods. Total phenolic, flavonoid, and proanthocyanidin contents were evaluated using spectrophotometric methods. The free radical scavenging activity of the acetone, methanol, and aqueous extracts of the fruit, leaf, and root were evaluated against DPPH+, ABTS+, and phosphomolybdenum. Alkaloid and saponin contents were also determined. Results. The acetone extract of the fruit revealed the highest polyphenol content when compared with the other solvent extracts analyzed. Total phenol content of the parts tested ranged from 3.66 ± 0.17 to 44.98 ± 3.41, flavonoid content ranged from 4.63 ± 3.33 to 401.33 ± 7.89, and proanthocyanidin content ranged from 8.84 ± 2.65 to 504 ± 36.6. Significant amount of alkaloids present was observed in the fruits, leaf, and root (10.68 ± 0.68, 14.12 ± 1.67, and 12.15 ± 4.74), respectively, while saponin content was 33.33 ± 11.55, 26.67 ± 11.55, and 20.00 ± 0.00 for the fruit, leaf, and root, respectively. Solvent extracts showed significant antioxidant activity, with acetone showing highest antioxidant ability in correlation with the polyphenol contents. Based on the IC50 values, acetone extract of the root revealed the best DPPH radical scavenging ability, the leaf aqueous extract had the highest IC50 value for ABTS, and the methanol extract of the leaf was best for phosphomolybdenum assays. Conclusion. This study suggests that fruit, leaf, and root of Cucumis africanus could be a potential source of natural antioxidant and justifies its use in ethnomedicine.

Antioxidant, anti-urease and anti-elastase activities of Usnea longissima Ach.

2018 ◽  
Vol 47 (3) ◽  
pp. 429-435
Author(s):  
Sinem Aydin ◽  
Kadir Kinalioğlu ◽  
Bahar Bilgin Sökmen
Keyword(s):  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Therapeutic Effects ◽  
Scavenging Activity ◽  
Total Antioxidant ◽  
Usnea Longissima ◽  
Ethanol Extracts

Lichens are complex associations composed of mycobiont and one or more algae or cyanobacteria which are living in symbiosis. Lichens are thought to possess therapeutic effects on many illnesses in worldwide. This study was performed to investigate antioxidant, anti-elastase and anti-urease activities of Usnea longissima Ach lichen. U. longissima was extracted with ethanol and ethyl acetate solvents. The antioxidant activities of the extracts of U. longissima were determined with methods such as 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity, copper reducing antioxidant capacity (CUPRAC), total antioxidant capacity, determination of total phenolic content and total flavonoid contents. The binding action of the DPPH and ABTS radicals, CUPRAC activity increases with concentration of the extracts. Ethanol extracts exhibited higher anti-urease and anti-elastase activity. Highest inhibition was found as 49.86% for elastase and 18.38% for urease. Results of the present study suggest that U. longissima extracts can be an alternative to synthetic antioxidant, anti-elastase and anti-urease agents.

scholarly journals CHARACTERIZATION OF ANTIOXIDANT ACTIVITY AND TOTAL PHENOLIC COMPOUND CONTENT OF BIRCH OUTER BARK EXTRACTS USING MICRO PLATE ASSAY

2017 ◽  
Vol 1 ◽  
pp. 197 ◽  
Author(s):  
Ilva Nakurte ◽  
Kristaps Stankus ◽  
Inguss Virsis ◽  
Aigars Paze ◽  
Janis Rizhikovs
Keyword(s):  
Betula Pendula ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Outer Bark ◽  
Dry Extract ◽  
Plate Assay ◽  
Ethanol Extracts

In modern plants, 2.7 to 2.8 m3 of solid volume veneer blocks are consumed to produce 1 m3 of plywood. After the hydrothermal treatment and debarking of blocks, waste bark is obtained, which makes up 12.5% of the wood mass, while 16-20% of bark is composed of birch outer bark (BOB). Recalculating, BOB makes up 2.0-3.4% of the veneer log mass. Bark is currently burned in boiler houses that is not rational. BOB contains large amount of valuable extractives (up to 34% from o.d. BOB) consisting of various secondary metabolites such as terpenes, flavonoids, hydrocarbons, polyphenols, tannins etc. BOB extractives exhibit antioxidant properties as well as wound-healing and anti-inflammatory activity. The objective of this paper was to compare amount of total phenolic content (TPC) and antiradical activity (ARA) in ethanol extracts from silver birch (Betula pendula Roth.) outer bark using micro plate assay. Among 11 fractions, fraction of average BOB had the highest DPPH free radical scavenging activity with IC50 of 39.28 μg/mL and the highest TPC 7.42 ± 0.52 g GAE/100 g of dry extract.

scholarly journals Phenolic Combination and Comparison of Antioxidant Activity in Three Different Alcoholic Extracts of Dracocephalum moldavica L.

2017 ◽  
Vol 5 (3) ◽  
pp. 199 ◽  
Author(s):  
Behnaz Aslanipour ◽  
Reza Heidari ◽  
Neda Farnad
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Reducing Ability ◽  
Ethanol Extracts ◽  
Phenolic And Flavonoid

Dracocephalum moldavica L. has been used as both treatment of coronary heart disorders and food supplements. In the present study, determination of total phenolic and flavonoid content in methanol, ethanol and methanol/ethanol extracts of leaf was studied in Iranian Moldavian balm for the first time. Antioxidant activity of the studied plant was also measured. The methanol extract had the highest phenolic and flavonoid content, anthocyanin, DPPH and H2O2 radical scavenging activity. The ethanol extract showed the least amount of all. The methanol/ethanol extract showed the highest amount in two oxides including nitric and superoxide radical scavenging activities; it also showed the highest Ferric Reducing Ability Power. The obtained chromatograms of the plant using High Performance Liquid Chromatography showed that the highest and the lowest found phenolic compounds were caffeic acid and vanilic acid, respectively. The results show that this plant is a suitable natural antioxidant to reduce the oxidative stress in human being.

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