scholarly journals A Green Ultrasound-Assisted Extraction Optimization of the Natural Antioxidant and Anti-Aging Flavonolignans from Milk Thistle Silybum marianum (L.) Gaertn. Fruits for Cosmetic Applications

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 304 ◽  
Author(s):  
Samantha Drouet ◽  
Emilie A. Leclerc ◽  
Laurine Garros ◽  
Duangjai Tungmunnithum ◽  
Atul Kabra ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Dry Weight ◽  
International Standards ◽  
In Vitro Assays ◽  
Milk Thistle ◽  
Silybum Marianum ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Silybum marianum (L.) Gaertn. (aka milk thistle) constitutes the source of silymarin (SILM), a mixture of different flavonolignans and represents a unique model for their extraction. Here we report on the development and validation of an ultrasound-assisted extraction (UAE) method of S. marianum flavonolignans follow by their quantification using LC system. The optimal conditions of this UAE method were: aqueous EtOH 54.5% (v/v) as extraction solvent, with application of an ultrasound (US) frequency of 36.6 kHz during 60 min at 45 °C with a liquid to solid ratio of 25:1 mL/g dry weight (DW). Following its optimization using a full factorial design, the extraction method was validated according to international standards of the association of analytical communities (AOAC) to ensure precision and accuracy in the quantitation of each component of the SILM mixture. The efficiency of this UAE was compared with maceration protocol. Here, the optimized and validated conditions of the UAE allowed the highest extraction yields of SILM and its constituents in comparison to maceration. During UAE, the antioxidant capacity of the extracts was retained, as confirmed by the in vitro assays CUPRAC (cupric ion reducing antioxidant capacity) and inhibition of AGEs (advanced glycation end products). The skin anti-aging potential of the extract obtained by UAE was also confirmed by the strong in vitro cell-free inhibition capacity of both collagenase and elastase. To summarize, the UAE procedure presented here is a green and efficient method for the extraction and quantification of SILM and its constituents from the fruits of S. marianum, making it possible to generate extracts with attractive antioxidant and anti-aging activities for future cosmetic applications.

scholarly journals A Green Ultrasound-Assisted Extraction Optimization of the Natural Antioxidant and Anti-aging Flavonolignans from Milk Thistle Silybum marianum (L.) Gaertn. Fruits for Cosmetic Applications

2019 ◽  
Author(s):  
Samantha Drouet ◽  
Emilie Leclerc ◽  
Laurine Garros ◽  
Duangjai Tungmunnithum ◽  
Atul Kabra ◽  
...  
Keyword(s):  
International Standards ◽  
Milk Thistle ◽  
Silybum Marianum ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Ultrasound Frequency ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
The Individual

Silybum marianum (L.) Gaertn. (aka milk thistle) constitute the almost exclusive source of silymarin, a mixture of different flavonolignans, and is thus considered as a unique model for their extraction. The present research deals with ultrasound-assisted extraction (UAE) of S. marianum flavonolignans and their quantification using LC system. The optimal conditions for UAE were: aqueous EtOH 54.5% (v/v) as solvent, applying an ultrasound frequency of 36.6 kHz during an extraction time of 60 min at 45°C with a liquid to solid ratio of 25:1 ml/g DW. Following optimization, the extraction method was validated according to international standards of the association of analytical communities (AOAC) in order to ensure its precision and accuracy for the quantitation of the individual silymarin components. The efficiency of UAE was compared with maceration protocol of the same duration. The optimized and validated conditions allowed highest extraction yields of flavonolignans in comparison to maceration. The antioxidant capacity of the extracts was confirmed by the CUPRAC assays and inhibition of advanced glycation end products. The skin anti-aging action was also confirmed toward the strong in vitro inhibition capacity of the obtained extract against collagenase and elastase enzymes. The procedure presented here allows a green efficient extraction and quantification of the main flavonolignans from the fruits of S. marianum with attractive antioxidant and anti-aging activities for future cosmetic applications.

scholarly journals Optimization of ultrasound-assisted extraction of procyanidins from perilla seed hull and their antioxidant activities in vitro

2019 ◽  
Vol 39 (2) ◽  
pp. 378-387 ◽  
Author(s):  
Hui-Zhen LI ◽  
Yong-Lan TAN ◽  
Zhi-Jun ZHANG ◽  
Yao-Yao XIA ◽  
Xiao-Jun LI ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Seed Hull ◽  
Ultrasound Assisted ◽  
Perilla Seed

scholarly journals Ultrasound-assisted extraction of Ca, K and Mg from in vitro citrus culture

2003 ◽  
Vol 14 (3) ◽  
pp. 470-474 ◽  
Author(s):  
Sandra C. C. Arruda ◽  
Adriana P. M. Rodriguez ◽  
Marco A. Z. Arruda
Keyword(s):  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

scholarly journals Antioxidant Capacity and Antigenotoxic Effect of Hibiscus sabdariffa L. Extracts Obtained with Ultrasound-Assisted Extraction Process

2020 ◽  
Vol 10 (2) ◽  
pp. 560
Author(s):  
Gregorio Iván Peredo Pozos ◽  
Mario Alberto Ruiz-López ◽  
Juan Francisco Zamora Nátera ◽  
Carlos Álvarez Moya ◽  
Lucia Barrientos Ramírez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Human Lymphocytes ◽  
Dry Weight ◽  
Extraction Process ◽  
Hibiscus Sabdariffa ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Antigenotoxic Effect

Hibiscus sabdariffa (Roselle) is in high demand worldwide due to its beneficial health properties owing to the polyphenols content, mainly in the flower calyx. The objective of this study was to find the best conditions (time and liquid: solid ratio) to extract polyphenols from Roselle using Ultrasound-Assisted Extraction (UAE) (40 kHz, 180 W), with ethanol how solvent; as well as determine the yield of phenols, anthocyanin, flavonoids, tannins, antioxidant activity (DPPH) and antigenotoxic effect (comet assay). A traditional solid-liquid extraction was applied as a reference. Extraction times of 40 and 60 min resulted in the highest polyphenols (13.019 mg GAE/g dry weight (dw)), flavonoids (4.981 CE/g dw), anthocyanins (1.855 mg Cya3GE/g dw), and tannins (0.745 CE/g dw) recoveries and an antioxidant activity (DPPH) of 74.58%. Extracts from white calyces contained similar amounts of phenols and flavonoids, but very little condensed tannins (0.049 CE/g dw) and practically no anthocyanins. Extracts from red and white calyces, showed antigenotoxic activity and repaired capacity of damage caused by mutagens in human lymphocytes.

Impact of ultrasound-assisted extraction and solvent composition on bioactive compounds and in vitro biological activities of thyme and rosemary

2020 ◽  
Vol 134 ◽  
pp. 109242 ◽  
Author(s):  
Paulo E.S. Munekata ◽  
Cristina Alcántara ◽  
Tihana Žugčić ◽  
Radhia Abdelkebir ◽  
María Carmen Collado ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Biological Activities ◽  
Solvent Composition ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

scholarly journals In vitro and in vivo antioxidant properties of bioactive compounds from green propolis obtained by ultrasound-assisted extraction

2019 ◽  
Vol 4 ◽  
pp. 100054 ◽  
Author(s):  
Renata Iara Cavalaro ◽  
Richtier Gonçalves da Cruz ◽  
Sebastien Dupont ◽  
Juliana Maria Leite Nóbrega de Moura Bell ◽  
Thais Maria Ferreira de Souza Vieira
Keyword(s):  
Bioactive Compounds ◽  
Antioxidant Properties ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

scholarly journals Green solvents and Ultrasound-Assisted Extraction of bioactive orange (Citrus sinensis) peel compounds

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Abigail Montero-Calderon ◽  
Clara Cortes ◽  
Ana Zulueta ◽  
Ana Frigola ◽  
Maria J. Esteve
Keyword(s):  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Orange Peel ◽  
Green Solvents ◽  
Ultrasound Assisted Extraction ◽  
Total Carotenoids ◽  
Carotenoid Concentration ◽  
Assisted Extraction ◽  
Physicochemical Evaluation ◽  
Ultrasound Assisted

Abstract Byproducts such as orange peel have potential uses because of their bioactive compounds, which are important for their potential to reduce the risk factors of diseases caused by aging. The lack of effective techniques and the high levels of pollution produced by the conventional extraction of bioactive compounds using organic solvents have highlighted the need to enhance the ‘green chemistry’ trend. This study evaluates the use of ultrasound to extract bioactive compounds from orange peel. The antioxidant capacity, phenolic content, ascorbic acid, total carotenoids, and HPLC profile of phenolic compounds from orange peel extracts were obtained by a physicochemical evaluation. The results demonstrate that the optimal conditions for the ultrasound-assisted extraction of bioactive orange peel compounds were a power of 400 W, a time of 30 min, and 50% ethanol in water. These conditions were used to obtain a total carotenoid concentration of 0.63 mg ß-carotene/100 g, vitamin C concentration of 53.78 mg AA/100 g, phenolic concentration of 105.96 mg GAE/100 g, and antioxidant capacity of ORAC = 27.08 mM TE and TEAC = 3.97 mM TE. The major phenolic compound identified in all orange peel extracts was hesperidin, with a maximum concentration of 113.03 ± 0.08 mg/100 g.

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